JPH04237463A - Theanderose-containing candy - Google Patents

Abstract

PURPOSE:To prepare a non- or low-cariogenic candy by including theanderose into a candy containing various carbohydrates as a sweetener. CONSTITUTION:A cariostatic candy is prepared by producing theanderose from glucan and sucrose using a glucosyl transferase originated from a microorganism of genus Mucor and adding the theanderose to a candy containing a carbohydrate as a sweetener.

Description

[Detailed description of the invention]

0001

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an anti-caries candy containing theanderose.

0002

BACKGROUND OF THE INVENTION In recent years, from the standpoint of oral hygiene, there has been a growing tendency to dislike giving candies containing large amounts of sucrose, which causes caries (tooth decay), to infants and children. Accordingly, interest in low-cariogenic sweeteners that can replace sucrose is increasing. Synthetic sweeteners are also available as this type of sweetener, but they have safety issues, and even if there is no problem, consumers have a strong tendency to dislike them psychologically, so glycyrrhizin Naturally derived advanced sweeteners such as stevioside and stevioside are attracting attention.

However, these sweeteners are inferior to sugar in terms of taste quality and the like. For example, glycyrrhizin has a sweetness level 50 to 100 times higher than that of sucrose, and 250 times higher in pure form, but has the disadvantage that its sweetness is slow-acting and leaves an unpleasant aftertaste. In addition, candies using carbohydrate-based low-cariogenic sweeteners such as maltitol and palatinose, which are relatively good in taste, have been developed.

[0004] However, although maltitol and palatinose are said to have low cariogenicity, they not only do not have the effect of actively inhibiting caries, but also have a cariogenicity comparable to or higher than that of starch. Candies made with this cannot be said to be cariogenic in the true sense.

[0005]

SUMMARY OF THE INVENTION An object of the present invention is to provide a cariogenic candy. The theanderose referred to in the present invention is a trisaccharide having the structure shown below.

[0006]

[Chemical formula 1]

[0007]

[Means for Solving the Problems] As a result of extensive research into solving the above-mentioned problems, the present inventors found that the addition of theanderose, which itself has a sweet taste and has the effect of suppressing dental caries. The present invention was completed based on the knowledge that a cariogenic-free candy can be obtained. That is, the present invention is an anti-caries candy containing various carbohydrates as a sweetener, which is characterized by containing theanderose produced from glucan and sucrose using glucosyltransferase derived from Mucor bacteria.

[0008] The various carbohydrates used in the present invention constitute all or part of the sweetener. Examples include sucrose, glucose, fructose, isomerized sugar, other sugars, sugar alcohols such as sorbitol and xylitol, and other sugars such as starch syrup. Natural or artificial sweeteners such as , stevioside, and aspartame can be used; however, in the production of candy for the purpose of preventing caries, it is desirable to use sweeteners that are as carious-free as possible.

[0009] Theanderose in the present invention constitutes part or all of the food additive added for the purpose of preventing dental caries. When used in combination with other food additives, any combination is possible as long as they do not interfere with each other's effects. Further, the mixing ratio with various carbohydrate sweeteners may be appropriately set depending on the shape of the product, its purpose, storage conditions, and the presence of other additives. In addition, as for its production method, it is produced from glucan and sucrose using glucosyltransferase derived from Mucor genus bacteria.
avanicus can be efficiently generated.

[0010] In addition to the above ingredients, colorants, fragrances,
It is of course possible to add acidulants, seasonings, and other additives within the scope of the purpose of the present invention.

[0011]

【Example】

0012

[Reference example 1] This reference example is Mucor javanic
Using the cell extract of US IFO 4570,
Transfers glucosyl groups from soluble starch to sucrose,
This is an example of obtaining a sweetener containing theanderose using membrane separation technology. (1) Mucor javanicus IFO
Preparation of cell extract from 4570 Mucor javanicus IFO 45
70 in 100 ml of natural medium [4 g of soluble starch,
Corn steep liquor (pH 5.5-5.8) 3g, N
aNO3 0.5g, KH2 PO4 0.1g, Mg
Shaking culture was carried out at 30° C. for 2 days in a 500 ml flask containing 0.05 g of SO4 .7H2 O and 0.05 g of KCl dissolved in distilled water to make 1 liter. Same culture solution 5m
1 was transplanted into a 500 ml flask containing 100 ml of the above natural medium, and cultured with shaking at 30° C. for 2 days. Bacteria were collected by filtration from 300 flasks cultured using the same method,
After washing with deionized water, it was stored at -20°C. The same bacterial cells were suspended in 5.1 liters of 1M acetate buffer (pH 5.3) containing 4M urea and extracted at 30°C for 48 hours. After removing bacterial cell residue from the same extract by filtration, the filtrate was heated to 0°C.
It was cooled to Next, 50% of acetone cooled to -20°C was added to the same filtrate.
% (v/v) while stirring. After removing the precipitate from the same solution by centrifugation, 1M CaCl
2 1.45 ml of aqueous solution was added. After adding polyethylene glycol 6000 to 20% (v/w) to the same extract, it was left at 4°C for 1 hour, and the resulting precipitate was centrifuged and dissolved in 50 ml of 0.05 M acetate buffer (pH 5.3). The same solution was used as a cell extract for subsequent operations. (2) Preparation of theanderose 0 containing 3% (w/v) soluble starch and 17% sucrose
．． Add Muco to 3 liters of 05M acetate buffer (pH 5)
0.3 ml of cell extract derived from r. After heating the obtained reaction solution at 100°C for 15 minutes, 3 g of activated carbon (Shirasagi A, manufactured by Takeda Pharmaceutical Company Limited) was added, and 5
Decolorization was performed by heating at 0° C. for 1 hour. After removing the activated carbon, it was treated with Amberlite IR-120 and Amberlite IRA-400 ion exchange resins. Next, concentration and dilution with water were repeated using an ultrafiltration membrane with a molecular weight fraction of 500 (Ultrafilter, manufactured by Advantech Toyo Co., Ltd.), resulting in 36% theanderose and 36% sucrose.
%, glucose 2%, soluble starch 27% after preparing a non-cariogenic or hypocariogenic mixture with a sugar composition of 7%.
Concentration to 0% (w/w) concentration yielded 214 g of non-cariogenic or less cariogenic solid.

Furthermore, the following glucose transfer reaction solution [acetate buffer containing 3% soluble starch and 17% sucrose (pH 5.0)] was prepared using the solution that had passed through the ultrafiltration membrane.
By repeating the same operation, it was possible to increase the utilization efficiency of sucrose in this method to 70% or more. In addition, 67 g of theanderose could be obtained by dissolving the above-mentioned non-cariogenic or low-cariogenic solid in distilled water and fractionating the solution in several batches using an activated carbon column (50 x 900 mm).

[0014]

[Example 1] Sucrose
67g Theanderose
67g starch syrup
66g of the above components were heated and melted to be sufficiently uniform. After cooling and solidifying, the powder was finely ground to prepare a feed containing this powder at a weight ratio of 56% as the sole carbohydrate source. This feed was used for S. mutans PS-
The 14 strains were given to 10 rats that were orally infected and kept for 58 days. As a result, the average caries score for rats was 3.1.
It was ±1.8.

[0015]

[Comparative Example 1] Sucrose
134g starch syrup
66g of the above components were heated and melted to be sufficiently uniform. After cooling and solidifying, the powder was finely ground to prepare a feed containing this powder at a weight ratio of 56% as the sole carbohydrate source. This feed was used for S. mutans PS-
The 14 strains were given to 10 rats that were orally infected and kept for 58 days. As a result, the average caries score of rats was 83.
It was 2±5.6.

[0016]

[Comparative Example 2] Starch
134g starch syrup
66g of the above ingredients were heated and mixed until thoroughly homogeneous. After cooling and solidifying, the powder was finely ground to prepare a feed containing this powder at a weight ratio of 56% as the sole carbohydrate source. This feed was used for S. mutans P
S-14 strain was given to 10 rats orally infected, and 58
It was kept for days. As a result, the average caries score for rats was 8.
．． It was 5±3.1.

[0017]

[Comparative Example 3] Sucrose
67g maltitol
67g starch syrup
Heat and melt 66g of the above ingredients,
It melted sufficiently uniformly. After cooling and solidifying, the powder was finely ground to prepare a feed containing this powder at a weight ratio of 56% as the sole carbohydrate source. This feed was used for S. mutans PS
-14 strain was given to 10 rats orally infected and kept for 58 days. As a result, the average caries score for rats was 13.
．． It was 5±2.8.

These results show that the candy containing theanderose has anti-caries properties. In addition, when the candies of the present invention before being crushed and the candies prepared in Comparative Examples 1 to 3 were subjected to a sensory test by 12 panelists regarding the refreshing taste, it was found that the candies of the present invention were better than those of the comparative examples. also obtained good results. The results are shown in Table 1.

[0019]

[Table 1]

[0020]

Effects of the Invention According to the present invention, by including theanderose as a component of a candy containing various carbohydrates as a main sweetener, a refreshing candy with a non-cariogenic taste can be obtained.

Claims (1)

[Claims] 1. An anti-caries candy containing various carbohydrates as a sweetener, characterized in that it contains theanderose produced from glucan and sucrose using glucosyltransferase derived from Mucor bacteria.