JPH0432079Y2 - - Google Patents

Description

[Detailed description of the invention] The present invention is a reagent applicator for microbial testing, and more specifically, a reagent used in microbial identification tests and minimum inhibitory concentration measurement tests for antibacterial agents, which can be used to simultaneously apply a number of reagents to a large number of culture media. This invention relates to a reagent needle for microbial testing that can be pierced.

In general, the identification of bacteria is complex and diverse, and the range of bacteria that can be identified continues to expand, especially as opportunistic infections are increasing. However, there are limits to the manual identification work performed one by one in a laboratory. Therefore, various types of simple identification kits have been frequently used in recent years to improve efficiency.

However, most of these seed kits require a considerable amount of skill and skill, as most of them require special forms and reaction methods that are different from conventional testing methods that examine the growth status of bacteria on live media. It has the disadvantage that it can easily cause confusion when determining results if one is not familiar with it.

In addition, when measuring the inhibitory concentration of antibacterial agents, it is essential to accurately and quickly measure the inhibitory concentration in order to take early clinical action. However, the dilution method is avoided because of its complicated procedure, and the simple disk method is currently often used in actual testing sites.

Therefore, the actual situation is that identification work and antibacterial testing, which should normally be carried out simultaneously in the laboratory under the same conditions, are carried out in different formats: the simple identification kit and the disk method.

Therefore, the inventor of the present invention has conducted various studies in order to eliminate such drawbacks of the conventional methods, and as a result, the original procedure for bacterial testing is to inoculate bacteria onto a medium, and after culturing, react with a reagent to identify and identify antibacterial agents. The present invention has been proposed, which can be applied to the method of measuring the minimum inhibitory concentration, allows identification work and the measurement of the minimum inhibitory concentration to be performed under the same conditions, and enables the measurement of the minimum inhibitory concentration by a simple and quick dilution method. This is what was put out.

That is, in the present invention, a plurality of downwardly-opening compartments are formed inside a container body consisting of a top plate and a side wall using a partition plate whose lower end is on the same plane as the lower end of the side wall. A reagent abutment device for microbial testing is characterized in that a downwardly projecting reagent abutment protrusion is provided on the same plane as or above the lower end of the side wall and the lower end of the partition plate.

The present invention will be further explained below with reference to drawings showing embodiments.

1 is a container body consisting of a top plate 1a and a side wall 1b, in which a plurality of compartments are formed that are open at the bottom with a partition plate 2 whose lower end is on the same plane as the lower end of the side wall 1b. . The shape of this vessel 1 is not limited to a rectangular shape, but may be circular, oval, etc., and therefore, the shape of each compartment is not necessarily limited to a rectangular shape.

Reference numeral 3 denotes a protrusion for sticking the reagent into the culture medium, which is installed so as to protrude downward into each compartment, and its lower end is on the same plane as or above the lower end of the vessel side wall 1b and the lower end of the partition plate 2. It is located in The specific attachment point of this projection 3 does not matter whether it is the top plate 1a or the partition plate 1b as long as it is in the compartment, but it can be attached to the top plate 1a as a straight projection, and it is easy to form and use. It is more convenient.

Further, the shape of the protrusion 3 may be any shape as long as it can attach the reagent and make contact with the culture medium, but for example, as shown in FIG. 5, a recess may be formed at the contact end. , those with spiral grooves or horizontal grooves formed on the outer peripheral wall, those with small holes facing inward from the spliced end, those with multiple cuts above the spliced end, and the spliced ends The length of the part may be a strip, or a combination of these as appropriate, and the length should be set upward from the lower end of the side wall 1b of the container and the lower end of the partition plate 2, in other words, to prevent mixing of reagents. It is more preferable to have a shorter dimension than the vessel side wall 1b and the partition plate 2.

Various biochemical reaction reagents and antibacterial agents can be attached to the projections 3 by various means, but for example, as shown in FIG. It is efficient to insert and attach each protrusion 3 into each container 6, since it is possible to attach a large number of reagents at the same time.

Reference numeral 4 denotes small holes provided in an appropriate number on the top plate 1a of the vessel, which facilitate the injection of secondary reaction reagents and liquid paraffin. Note that 5 is a plug for the small hole 4.

In the present invention, the material of the container body 1 and the protrusion 3 may be metal, glass, synthetic resin, etc., but
Synthetic resins are advantageous in terms of moldability and handling.

As the present invention is constructed as described above, if the present invention is used, the stinger protrusion 3 can be removed by simply covering the culture medium inoculated with bacteria according to the conventional general bacterial testing procedure. Since a large number of reagents can be applied at the same time, and the culture medium can be fractionated for each reagent site by the partition plate 2, extremely simple and accurate microbial testing can be performed without being inhibited by other reactions. It is possible.

In addition, by using the reagent capacitor of the present invention, various identification reagents and antibacterial agents of various concentrations can be attached to the needle protrusion, allowing identification work and minimum inhibitory concentration measurement using the dilution method to be performed under the same conditions and extremely efficiently. It is something that can be done.

In addition, in the reagent pricking device of the present invention, various reagents are applied to the pricking protrusion 3 in advance and then dried. When in use, microorganisms can be tested more efficiently by filling a container 9 with a culture medium 10 or a fractionated culture medium 11 in advance to form a kit, which has a shape suitable for covering the cap of the present invention's needle. Therefore, it is advantageous. Alternatively, in use, various reagents may be attached to the contact protrusion 3 and then the container 9 containing the culture medium may be immediately covered.

[Brief explanation of the drawing]

The drawings show an embodiment of the present invention; FIG. 1 is a perspective view, FIG. 2 is a cross-sectional view taken along line A-A, FIG. 3 and 4 are perspective views of other embodiments, and FIG. Front explanatory diagrams of various embodiments of the contact protrusion (however, only figure 7b is
FIG. 6 is an explanatory front view showing an example of a method of attaching a reagent to the stinging projection, and FIG. 7 is an explanatory perspective view of an example of a culture medium filling container. 1... Vessel body, 1a... Top plate, 1b... Side wall, 2
...Partition plate, 3...Application protrusion, 3a...Recess,
3b...Spiral groove, 3c...Horizontal groove, 3d...Conical small hole, 3e...Slit, 3f...Strip portion, 4...
Small hole, 9...container, 10...medium.

Claims (1)

[Scope of utility model registration request] A plurality of downward-opening compartments are formed inside the container body consisting of a top plate and a side wall by a partition plate whose lower end is on the same plane as the lower end of the side wall, and inside each compartment, the lower end is located on the same plane as the lower end of the side wall and the partition. A reagent pricker for microbial testing, characterized in that it is provided with a downwardly protruding reagent pricking protrusion that is on the same plane as or above the bottom end of the plate.