JPH051279Y2 - - Google Patents

Description

[Detailed Description of the Invention] The present invention relates to a microbial testing tool, and more particularly to a microbial testing tool that can simultaneously add a large number of test reagents to a culture medium.

In general, fixation of bacteria is complicated, and there are limits to the manual identification work performed one by one in a laboratory, so various types of simple identification kits have been frequently used in recent years to improve efficiency.

However, all of these types of simple identification kits require considerable skill, and inexperienced users have the disadvantage of easily causing confusion when determining the results.

In addition, for early clinical treatment, it is essential to accurately and quickly measure the inhibitory concentration of antibacterial agents (drug susceptibility testing). is avoided due to its complicated procedure, and the simple disk method for measuring the inhibition circle is currently frequently used in actual inspection sites.

It is preferable to perform bacterial identification and drug susceptibility testing at the same time under the same conditions in a laboratory. However, the above-mentioned known simple identification kits are only capable of identifying bacteria, and cannot simultaneously perform identification tests and drug susceptibility tests easily and quickly under the same conditions.

Therefore, the inventor of the present invention has conducted various studies in order to overcome these conventional drawbacks. As a result, the inventors have identified the bacteria by inoculating the bacteria onto a medium, reacting with a reagent after culturing, and have determined the minimum inhibitory concentration of the antibacterial agent. They devised the present invention, which can be applied to a method of measuring . By using the microbial testing device of the present invention, it is possible to simultaneously identify bacteria and measure the minimum inhibitory concentration using the dilution method under the same conditions, simply and quickly.

That is, the present invention provides a microorganism characterized in that a plurality of compartments are formed using partition plates inside a container consisting of a top plate and a side wall, and a reagent member is attached to the side wall or top plate in each compartment. It is an inspection tool.

The present invention will be further explained below with reference to drawings showing embodiments.

The container body 1 consists of a top plate 3 and a side wall 4, and a plurality of compartments are formed inside thereof by partition plates 2. The shape of this vessel 1 is not only rectangular, but also circular,
The shape of each compartment is not necessarily limited to a rectangular shape, regardless of whether it is an ellipse or the like.

Note that the partition plate 2 may be formed so as to form an air space between it and the side wall 4 as shown in FIG. 1A, or without forming an air space between it and the side wall as shown in FIG. 1B. good. In the case shown in FIG. 1A, a medium container 13 smaller than the container 1 is used so that the side wall of this container is accommodated in the air space, and in the case shown in FIG. 1B, the medium container 13 is larger than the container 1. Preferably, a culture medium container 13 is used. This vessel is used by being set as a lid on a culture medium container during culture.

A reagent member 6 is attached to each compartment. The reagent member 6 may be attached to the partition plate 2 as shown in FIG. 2 or the top plate 3 as shown in FIG. When attached,
It is preferable to use a reagent member that is dissolved and dripped by appropriate heat or the like.

The specific structure of this reagent member 6 includes, for example, a layered structure with an appropriate substrate 5 as shown in FIG. 2A and FIG. 3 F, G, or H, and a layered structure with an appropriate substrate 5 as shown in FIG. A parallel structure, a mixture 7 of a substrate and a reagent as shown in Figure 2C, or a support 8 such as paper impregnated with an appropriate substrate and reagent as shown in Figure 2D. Examples include things. In addition, when attaching the reagent member 6 to the top plate surface of the container, it is effective to attach it via a deposit 9 that is immediately heat-soluble at 35° C. or higher, as shown in FIG. 3 F and G, for example. It is true.

Figures 2C and 3H show a hole in a part of the top plate and a stopper 10 attached to the hole, which is used to block air during culture when anaerobic bacteria are to be tested. This is effective when injecting liquid paraffin or the like into the upper layer of the culture, or when it is necessary to add a new reagent after culturing to identify some bacteria. Further, FIG. 2E shows an example in which a thermofusible material 11, which replaces the role of the liquid paraffin described above, is used instead of providing a stopper to the top plate.

4A to 4E show structural examples in which the partition plate 2 formed inside the container body and the bottom of the container 13 are fitted together. The testing tool of the present invention is used by setting the lid on the culture medium container 13 immediately after inoculating the culture medium 12 with a specimen bacterial sample. On this occasion,
By employing the above-mentioned fitting structure, the plurality of compartments are independently separated from each other, making it possible to prevent mutual contamination of reagents during culture.

Further, f to i in FIG. 5 indicate partition plates 2 which are advantageous for attaching reagent members to the partition plate 2.
This figure shows an example of the shape.

Although the material of the vessel 1 in the present invention is not particularly limited, synthetic resin is advantageous in terms of moldability, handling, and inspection.

In addition, when using the microbial testing tool of the present invention, the container 13 is filled with the medium 12 in advance to form a kit, or a liquid medium or a mixture of a liquid medium and a sample bacterial solution prepared separately in an empty container at the time of testing is used. It can be used by pouring it in. Note that the container 13 can also be used for fractionation, and different specimens and culture media can be used for different fractions.

Since the present invention is constructed as described above, if the present invention testing tool is used, it is only necessary to cover the culture medium inoculated with bacteria according to the conventional general bacterial testing procedure, and the reagent member 6 can simultaneously A large number of reagents can be applied, and since the culture medium can be fractionated for each reagent site by the partition plate 2, it is not inhibited by other reactions and can perform extremely simple and accurate microbial tests. It is.

In addition, by using the test tool of the present invention, the reagent components are composed of various identification reagents and antibacterial agents of various concentrations, so that identification work and minimum inhibitory concentration measurement using the dilution method can be performed simultaneously and extremely efficiently. can be done.

[Brief explanation of the drawing]

The drawings show an embodiment of the present invention, and FIG.
and B are perspective views of the vessel body, Figures 2A to D are cross-sectional explanatory views showing examples of attaching reagent members to the partition plate, and Figure 3F
~H are cross-sectional explanatory views showing examples of attaching reagent members to the top plate, Figures 4 a-e are cross-sectional views showing examples of the fitting structure between the container bottom and the partition plate, and Figures f-i are the partitions. It is a cross-sectional explanatory view showing an example of the shape of a plate. 1... Vessel body, 2... Partition plate, 3... Top plate, 4
... side wall, 6 ... reagent member, 12 ... culture medium.

Claims (1)

[Scope of utility model registration request] A microbial testing tool characterized in that a plurality of compartments are formed by partition plates inside a container consisting of a top plate and a side wall, and a reagent member is attached to the side wall or top plate in each compartment.