JPH043365B2 - - Google Patents

Description

[Detailed description of the invention]

[Industrial Application Field] The present invention relates to pharmaceutical agents, particularly cardiovascular and metabolic agents, which contain as an active ingredient a physiologically active substance contained in Japanese ginseng. [Prior Art] Conventional medicines containing plant ingredients as active ingredients have long been known as Chinese herbal medicines. [Problems to be solved by the invention] Many conventional Chinese herbal medicines each have unique active ingredients, and are used for the treatment and prevention of specific diseases, but the range of indications and uses are limited. There are various drawbacks, such as the amount and combination with other drugs. Furthermore, many of the medicinal plants used as raw materials are difficult to obtain due to the cultivation environment. [Means for Solving the Problems] The present invention provides that the ingredients contained in the roots and stems of Japanese mountain ginseng, which is a new crop belonging to the cultivation lineage of Inutoki, a perennial plant of the Umbelliferae family, have been shown to be effective against human circulatory and metabolic systems. They discovered that this Japanese ginseng is a plant that can be cultivated in Japan, and completed the present invention. The present invention is a pharmaceutical composition for improving liver function and for antihyperlipidemia, which contains powder or extract of Japanese mountain ginseng. The Japanese mountain ginseng used in the present invention is from the Umbelliferae family (Umbellaceae).
It is a cultivated plant of Angelica shikokiana Makino, which is a perennial plant of the Japanese ferae, and the length of the main stem extension at full maturity is approximately 120 cm.
cm to 130 cm, with one inflorescence growing epiphytically at the apex of the main stem and lateral branches, making it a compound umbel. The diameter of the inflorescence on the main stem is about 20 cm, and it blooms around June. In the present invention, the above-mentioned Japanese ginseng is mainly used as an active ingredient by drying the stem or root thereof and turning it into powder, or by extracting the stem or root part with an organic solvent such as ethyl acetate. The pharmaceutical agent of the present invention is provided as a medicine or a health food by drying the stem and root part of the Japanese mountain ginseng and making it into a tablet, pill, capsule, or other formulation. When making tablets, pills, and capsules, excipients such as starch, sugar, mannitrate, etc., cellulose derivatives such as carboxymethyl cellulose, alginate, A preparation is prepared using a conventional method by adding binders such as gelatin and polyvinylpyrrolidone, and lubricants such as glycerin as appropriate. In addition, when making it into a health food, in addition to the main ingredients, garlic, egg yolk oil, various vitamins, etc. used in other health foods are added as appropriate, and the above excipients, binders, lubricants, etc. are added, and tablets, pills, etc. are added. and capsules. The pharmaceutical composition of the present invention can be prepared into tablets, pills, capsules, etc. by adsorbing the Japanese ginseng extract to the excipient or adsorption carrier such as kaolin or bentonite. The ingredients of Japanese ginseng, which is the active ingredient of the present invention, are bergapten, psoralen, 3'-acetoxy-4'-
angeloxy-3',4'-dihydroceserine and
3'-acetoxy-4'-senesioxy-3',4'-dihydroxyseserine. [Example] The stems and roots of Japanese mountain ginseng are dried and powdered. Fill capsules with 410 mg of this powder, garlic powder and 90 mg of egg yolk oil. The capsules are usually administered orally by adults in 3 to 6 capsules per day. [Effects of the Invention] Japanese ginseng, which is the active ingredient of the present invention, has the effect of suppressing the effects of noradrenaline and catecholamines, and further has the effect of preventing fat accumulation in the liver and lipid peroxide accumulation in the body. It is. Next, we will describe a test that shows its effectiveness. Test Example 1 Effect of the drug of the present invention on liver damage and hyperlipidemia A Experimental materials and methods A Experimental materials 1 Drug used: Japanese mountain ginseng 2 Materials used: Wistar male rats (weight 200-220 g) Entered from Charles River, room temperature 23℃±1℃, humidity 55%±5
The experiments were conducted using rats that were preliminarily bred for one week at 100% of the rats and were healthy and had good coats. 3 Lipid peroxide: When corn oil is heated at 180° to 200°C for 1 hour while injecting oxygen, lipid peroxide increases tenfold. An experiment was conducted using the peroxidized corn oil thus prepared. B. Experimental method 1. Effect of Japanese ginseng on rats administered with lipid peroxide Male Wistar rats (body weight
340~360g) peroxidized corn oil per time between 9am and 10am.
It was orally administered for 11 days. Animals were sacrificed 1.5 hours after the final administration, and blood and liver were collected immediately. The experimental groups were as follows. Normal group: Solid feed manufactured by Oriental Yeast Co., Ltd. and water were freely available. Control group: The animals were given free access to solid feed manufactured by Oriental Yeast Co., Ltd. and water, and peroxidized corn oil was administered at 3 ml/animal once a day. Test group 1: The animals were given solid feed manufactured by Oriental Yeast Co., Ltd. and water ad libitum, and Japanese mountain ginseng was suspended in peroxidized corn oil at a concentration of 200 g/Kg and forcibly administered once a day. . Test group 2: The animals were given solid feed manufactured by Oriental Yeast Co., Ltd. and water ad libitum, and Japanese mountain ginseng was suspended in peroxidized corn oil at a concentration of 100 mg/Kg, and the animals were forcibly administered orally once a day. did. 2. Plasma enzymes, lipids, and liver lipids were measured using the following reagents. Serum transamylase (GOT,
GPT) S, TA test (Wako) Neutral fat (T, G) Mixed solvent extraction method Total cholesterol (T, C) Cholesterol B test (Wako) Lipid peroxide (Lipoperoxide) TBA method HDL-Cholesterol (HDL-Ch ₀ ) HDL-C Set (Daiichi Kagaku Yakuhin) 3. Experiments and Experimental Results Rats were divided into 4 groups, and the following experiments were conducted for each group. One group of five rats was given 11 days without administration of peroxidized corn oil or Japanese ginseng.
The group kept for one day was defined as the normal group. A group of 4 rats in each group was forcibly orally administered 3 ml of peroxidized corn oil once a day for 11 days as a control group. A test was conducted on a group of 4 rats in which 200 mg/Kg of Japanese mountain ginseng was suspended in peroxidized corn oil and 3 ml/rat was forcibly administered orally once a day between 9 and 10 a.m. for 11 days. Group 1 was selected. 1 group of 4 rats were given 100 mg/Kg of Japanese mountain ginseng suspended in peroxidized corn oil.
Test group 2 was a group to which 3 ml/mouse was forcibly administered orally once a day between 9 and 10 a.m. for 11 days. Serum GOT, GPT, total cholesterol, neutral fat, lipid peroxide, and HDL cholesterol of rats in each of the above groups were measured. The results were as shown in Table 1.

[Table] As is clear from the above results, serum GOT was significantly reduced in the test group administered with Japanese ginseng compared to the control group administered only with corn oil. The results of measuring liver lipids are shown in Table 2.

[Table] As is clear from the above results, neutral fat (T.
G) showed a marked decreasing trend in test group 1, total cholesterol (TC) significantly decreased in the test groups, especially test 1 group, and lipid peroxide showed a decreasing trend in the test group. As described above, the pharmaceutical composition of the present invention is effective in improving mild liver damage and fat accumulation in the liver caused by administration of lipid peroxide. In addition, it is one of the ingredients contained in Japanese mountain ginseng.
3'-acetoxy-4'-angeloxy-3',4'-dihydroceserine and 3'-acetoxy-4'-senecyoxy-3',4'-dihydroceserine are responsible for lipolysis by adrenaline, which is a counterhormone to insulin. The fact that it inhibits only adrenaline, which is the sympathetic system, and has no effect on the action of insulin, which is connected to the parasympathetic system, means that it has no effect on insulin-induced fat synthesis.
The physiological effect of adrenaline is that constriction of blood vessels causes high blood pressure, and it improves various symptoms such as hyperglycemia caused by increased adrenaline secretion due to stress.

Claims (1)

[Claims] 1. A pharmaceutical composition for improving liver function and for antihyperlipidemia, characterized by containing Japanese ginseng as an active ingredient.