JPH0436433Y2 - - Google Patents

Description

[Detailed explanation of the invention] A. Purpose of the invention (industrial application field) This invention involves centrifuging the solids contained in a liquid and smearing them onto a glass slide in order to make a microscopic specimen of the solids contained in the liquid. This invention can be used in a holder used to support a centrifugal smear cell used in a centrifugal separator.

(Prior art) For pathological research and diagnosis, for example, when removing red blood cells from the blood of a subject, the sixth method is used to separate solids contained in a liquid and smear it on a slide glass. A centrifugal smear cell as shown in the figure was used.

This centrifugal smear cell (hereinafter simply referred to as cell) 1 has a container tube 3 with an upwardly bent injection port coupled to a substrate 2, and a hole 4 of the container tube 3 is passed through the substrate 2 to the opposite side. It is left open. 5 and 6 are reinforcing ribs.

To perform centrifugal smearing, as shown in FIG. 9), which is then attached to a centrifuge as described below. The packing plate 7 is provided with holes 10 corresponding to the holes 4 of the cells 1.

To attach a cell, etc. to the holder 9, as shown in FIG. While pressing against the plate 11, the spring 12 is engaged with a hook (not shown) provided on the holder, so that the cell 1 and the like are connected to the holder 9 so as not to be separated.

The holder 9 with the cells 1 etc. connected in this way is attached to the rotor 14 of the centrifugal separator 13, as schematically shown in FIG. When the is rotated at high speed, the solid content centrifuged from the liquid to be treated in the holes 4 of the cell 1 adheres to the slide glass surface, and the liquid passes through the liquid-permeable packing plate 7 and is pushed out of the cell. Because it will be done,
Thereafter, the rotation of the roller 14 is stopped, the holder 9 is taken out from the centrifuge, the restraining spring 12 is removed, the slide glass 8 is taken out, and this is transferred to a microscope specimen preparation process.

Conventionally, the holder 9 was made of a metal plate.
In many cases, the cell 1 was attached using a restraining spring 12, but the inventor devised a method of molding the cell and holder with synthetic resin, as shown in Fig. 9 (1980).
Utility Model Registration Application No. 174240).

In FIG. 9, the cell 15 has a container tube 18 that communicates with a passage tube 17 that opens on the lower surface of the substrate 16, has two locking protrusions 19 formed at the left end of the substrate 16, and has two locking protrusions 19 formed at the right end of the substrate 16. A locking protrusion 20 and a handle 21 are formed on the holder. Substrate 16, passage tube 1
7. The container cylinder 18 is connected by reinforcing ribs 5 and 6. 22 is a lid provided with a small gap through which air passes. This cell 15 has not only moldability but also
It is made of synthetic resin such as nylon 66 in order to give the cell 6 strength and elasticity and to function as the restraining spring 12 of a conventional cell.

The holder 23 has a rectangular plate 24 with raised edges 25 on three sides.
A recess 26 is formed at the lower end (left end in FIG. 9) when mounted on a centrifuge, and two inclined grooves 27 connect this to the opposite right end. A cell locking protrusion 1 is provided on the folded edge 25 at the left end.
A hole 28 is bored into which the hole 9 is inserted. A hook piece 30 is connected to the right end of the rectangular plate 24, which has no folded edges, via two flexible thin arms 29. A hook 31 is formed at the upper end of the hook piece 30.

To use this cell 15 and holder 23, place the slide glass 8 and packing plate 7 on the rectangular plate 24 of the holder 23 as shown in FIG.
5, the locking protrusion 19 at the left end in FIG. 9 is inserted into the hole 28 of the holder.
When the locking protrusion 20 at the right end in FIG. 9 is pushed to the right while pushing the packing plate 7 with the board 16, the lock piece 30 is slightly tilted and passed through the position of the key 31. is restored due to the elasticity of the thin arm 29, and the locking protrusion 20 engages with the key 31, and the cell 15 and the holder 23 are attached to the slide glass 8.
etc. are retained and combined. So this holder 23
is attached to the centrifuge 13 (with the recess 26 facing down) as shown in FIG. 8, and centrifuged.

The liquid that flows into the holder through the liquid-permeable packing plate 7 during centrifugation enters the inclined groove 27 and enters the recess 26 along the inclined groove 27 due to the component of centrifugal force and gravity. It accumulates here.

Conventionally, in order to collect this liquid, a liquid absorbent such as blotting paper or a sponge is placed in the recess 26 to absorb the liquid, and after removing the slide glass from the holder,
This liquid absorption was discarded.

(Problem to be solved by the invention) If liquid absorption is put into the recess 26 of the holder 23 and the liquid separated from the material is absorbed, the separated liquid can be collected well, but the preparation for liquid absorption is , insertion into the holder, removal, and disposal are troublesome, and a lot of time is wasted.

B. Structure of the invention (Means for solving problems) This invention consists of a collection of fine voids in the recess 26 of the holder 23 molded with synthetic resin, where the separated liquid collects. A cell for centrifugal smearing is obtained by forming a projecting piece a that forms a liquid space integrally with a holder.

(Function) The liquid that is pushed out into the holder during centrifugation and tries to flow into the recess 26 enters the liquid suction space and is captured in this space due to surface tension, and the holder is removed from the centrifuged liquid and placed on the slide glass. It is possible to sufficiently prevent liquid from flowing out of the holder during operations such as removing it from the holder.

After use, by washing the holder with water, the liquid that has entered the liquid suction space can be easily washed away.

Since the liquid suction space is created by a protrusion formed integrally with the holder, the liquid suction space can always be handled together with the holder, which eliminates the hassle of using a conventional holder and separate liquid absorbent. There's nothing unpleasant about it.

(Example) Figures 1 and 2 show one example of the present invention,
FIG. 1 is a plan view of the holder 23, and FIG. 2 is a sectional view taken along line AA in FIG.

A liquid absorption space 32 is provided in the recess 26 following the rectangular plate 24.
A checkerboard-shaped projecting wall 33, which is one form of the projecting piece a forming the holder 23, is formed integrally with the holder 23. The height of the projecting wall 33 is made lower than the depth of the recess 26, as shown in FIG. The structure of the other parts of the holder 23 is similar to that shown in FIG.

A slide glass and a packing plate are attached to this holder as shown in FIG. 7, and when it is attached to a centrifuge 13 and centrifuged, the liquid flowing into the holder through the liquid-permeable packing plate 8 flows through the inclined groove 27. The liquid passes through and enters the recess 26, and is collected in the liquid suction space 32 formed by the protruding wall 33 in a grid pattern. This liquid absorption space 32
The volume of should be large enough to accommodate the liquid to be separated.

The separated liquid that has entered this liquid absorption space adheres to the projecting wall 33 due to surface tension and becomes difficult to flow out from the recess 26. Therefore, after centrifugation is completed, the holder is removed from the centrifuge, the cell 15 is removed, and the slide glass 8 There is no need to worry about the separated liquid flowing out during the extraction operation.

After removing the slide glass, the cell 15 and holder 23 can be washed with water, and the separation liquid accumulated in the recess 26 is discharged at this time.

Instead of forming a projecting wall 33 as a projecting piece a integrally with the holder 23 to form a liquid suction space as shown in FIG. 1, a number of short rods 36 are used as a projecting piece a as shown in FIG. A liquid absorbing space may be formed between the short rods 36 by planting them in close proximity to each other in the recess 26, or as shown in FIGS. They can be formed in the recess 26 in close proximity to form a liquid suction space. In this case, the portion between the small recesses 37 becomes a protrusion a for creating a liquid suction space.

C. Effects of the invention (1) Since the liquid suction space for collecting the separated liquid is formed in the recess 26 by the protrusion a, the separated liquid can be prevented from flowing out during the operation of removing the slide glass.

(2) The protrusion a that forms the liquid absorption space can be molded at the same time as the holder is molded from synthetic resin.

(3) There is no need to prepare a liquid absorbent such as a sponge or blotting paper, insert it into the holder, and then take it out and dispose of it after centrifugation, as in the conventional method.

(4) The separated liquid can be easily disposed of and cleaned by washing with water.

[Brief explanation of the drawing]

1 to 5 show embodiments of the present invention, FIG. 1 is a plan view of the first embodiment, FIG. 2 is a sectional view taken along line A-A in FIG. Fig. 4 is a plan view of the main part of the third embodiment, Fig. 5 is B of Fig. 4.
-B cross-sectional view, Figure 6 and subsequent figures show the conventional technology, Figure 6 is a perspective view of the cell, Figure 7 is a vertical cross-sectional view showing the state where the cell is attached to the holder, and Figure 8 shows the state of centrifugation. The schematic longitudinal sectional view and FIG. 9 are perspective views of a synthetic resin cell and a holder. 1... Cell, 2... Substrate, 3... Container cylinder, 4...
... Hole, 5, 6 ... Reinforcement rib, 7 ... Packing plate, 8 ... Slide glass, 9 ... Holder, 10
...hole, 11 ... rectangular plate, 12 ... restraining spring, 1
3... Centrifugal separator, 14... Rotor, 15... Cell, 16... Substrate, 17... Passage tube, 18... Container tube, 19, 20... Locking protrusion, 21... Handle,
22... Lid, 23... Holder, 24... Rectangular plate,
25... Folded edge, 26... Recess, 27... Inclined groove, 28... Hole, 29... Thin arm, 30... Key piece, 31... Key, 32... Liquid absorption space, 33...
Projection wall, 34...passage, 35...step, 36...short rod, 37...small recess, a...protrusion.

Claims (1)

[Scope of utility model registration request] A centrifugal smear cell holder for stacking a slide glass 8, a liquid-permeable packing plate 7 with holes 10, and a cell substrate 16 and attaching it to a centrifuge, and a rectangular plate 24 on which a slide glass is placed;
Adjacent to this, there is a recess 26 for accommodating the separated liquid, and within the recess 26, a projecting piece a is integrally formed, which forms a liquid absorption space that is made up of a collection of fine voids and that adsorbs and collects the separated liquid. Cell holder for centrifugal smearing.