JPH0610281Y2 - Centrifugal smear filter paper - Google Patents
Centrifugal smear filter paperInfo
- Publication number
- JPH0610281Y2 JPH0610281Y2 JP1985193538U JP19353885U JPH0610281Y2 JP H0610281 Y2 JPH0610281 Y2 JP H0610281Y2 JP 1985193538 U JP1985193538 U JP 1985193538U JP 19353885 U JP19353885 U JP 19353885U JP H0610281 Y2 JPH0610281 Y2 JP H0610281Y2
- Authority
- JP
- Japan
- Prior art keywords
- centrifugal
- cell
- filter paper
- liquid
- substrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000007788 liquid Substances 0.000 claims description 34
- 239000011521 glass Substances 0.000 claims description 23
- 239000000758 substrate Substances 0.000 claims description 22
- 230000002093 peripheral effect Effects 0.000 claims description 4
- 239000005871 repellent Substances 0.000 claims description 4
- 210000004027 cell Anatomy 0.000 description 33
- 239000007787 solid Substances 0.000 description 16
- 238000012856 packing Methods 0.000 description 11
- 238000000926 separation method Methods 0.000 description 6
- 238000005119 centrifugation Methods 0.000 description 5
- 239000012466 permeate Substances 0.000 description 3
- 230000007423 decrease Effects 0.000 description 2
- 230000003014 reinforcing effect Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000004809 Teflon Substances 0.000 description 1
- 229920006362 Teflon® Polymers 0.000 description 1
- 238000005452 bending Methods 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000002542 deteriorative effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000002940 repellent Effects 0.000 description 1
- 229920002545 silicone oil Polymers 0.000 description 1
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Description
【考案の詳細な説明】 ａ．考案の目的 （産業上の利用分野） この考案に係る遠心塗抹用濾紙は、液体中に含まれる固
形分の顕微鏡標本を作る為に、固形分を遠心分離してス
ライドガラス上に塗抹する場合に、遠心塗抹用セルと上
記スライドガラスとの間に挟持して使用する。Detailed Description of the Invention a. Purpose of the Invention (Industrial field of application) The centrifugal smear filter paper according to the present invention is used for centrifuging a solid content and smearing it on a slide glass in order to make a microscopic specimen of the solid content contained in a liquid. , It is used by sandwiching it between the cell for centrifugal smearing and the slide glass.
（従来の技術） 病理研究や診断の為、被検者の血液から赤血球を取出す
場合の様に、液体中に含まれる固形分を分離してスライ
ドガラス上に塗抹するのに、遠心塗抹用セルを使用し
て、上記液体中の固形分をスライドガラス上に塗抹する
事が行われている。(Prior art) Centrifugal smear cell is used for separating solids contained in liquid and smearing them on a slide glass for removing red blood cells from the blood of a subject for pathological research and diagnosis. The solid content of the above liquid is smeared on a slide glass by using.
遠心塗抹用セル（以下、単にセルと称する）１は第２図
に示すように、平板状の基板２に、上方に開口する注入
口を有する容器筒３を結合し、この容器筒３の内側に連
通する孔４を基板２の反対側に開口させたものである。
５、６は容器筒３と基板２との連結部に設けた補強リブ
である。As shown in FIG. 2, a centrifugal smearing cell (hereinafter, simply referred to as a cell) 1 is formed by connecting a plate-shaped substrate 2 with a container cylinder 3 having an inlet opening upward, and inside the container cylinder 3. The hole 4 communicating with the substrate 2 is opened on the opposite side of the substrate 2.
Reference numerals 5 and 6 are reinforcing ribs provided at the connecting portion between the container tube 3 and the substrate 2.
遠心塗抹を行なうには、第３図に示した様に、先ずこの
セル１の基板２を、ゴム板等のパッキング板７を介して
スライドガラス８に重ね、このスライドガラス８と共に
ホルダ９に取付ける。パッキング板には、セル１の孔４
に対応する孔１０を明けてある。In order to perform centrifugal smearing, as shown in FIG. 3, the substrate 2 of the cell 1 is first placed on a slide glass 8 via a packing plate 7 such as a rubber plate, and is attached to a holder 9 together with the slide glass 8. . The packing plate has holes 4 in the cell 1
The hole 10 corresponding to is opened.
ホルダ９は、第４図に示すように、三方に折立縁１１を
形成した矩形の金属板の一端部に針金状の抑えばね１２
の基端部を枢着する事で構成されており、上記抑えばね
１２の中間部を折立縁１１に形成したフック１３に係止
する事でセル１の基板２とこの基板にパッキング板７を
介して重ね合わされたスライドガラス８とを固定出来る
様に構成している。As shown in FIG. 4, the holder 9 has a wire-shaped holding spring 12 at one end of a rectangular metal plate having three raised edges 11.
It is constructed by pivotally connecting the base end of the cell 1, and by locking the intermediate part of the pressing spring 12 to the hook 13 formed on the folded edge 11, the substrate 2 of the cell 1 and the packing plate 7 are attached to this substrate. It is configured so that the slide glass 8 that is overlapped with it can be fixed.
抑えばね１２によってセル１等を結合したホルダ９は、
第５図に略示するように、遠心分離機１４に取付け、各
セル１の容器筒３内に適量の被処理液を注入し、回転軸
１５を高速回転（約３０００rpm）させる事で遠心塗抹
処理を行なう。セル１をこの様に高速回転させると、セ
ル１の容器筒３の中の被処理液から遠心分離された固形
分がスライドガラス面に付着する。この為、所定時間回
転軸１５を回転させた後、この軸１５の回転を止め、遠
心分離機からホルダ９を取出してセル１の容器筒３内に
残留する液分を流し出し、抑えばね１２を外してスライ
ドガラス８を取出せば、このスライドガラスの表面に顕
微鏡により観察すべき固形分が残留する。The holder 9 in which the cells 1 and the like are connected by the pressing spring 12 is
As schematically shown in FIG. 5, it is attached to a centrifuge 14, an appropriate amount of liquid to be treated is injected into the container cylinder 3 of each cell 1, and the rotary shaft 15 is rotated at high speed (about 3000 rpm) to perform centrifugal smearing. Perform processing. When the cell 1 is rotated at such a high speed as described above, the solid content centrifugally separated from the liquid to be treated in the container cylinder 3 of the cell 1 adheres to the surface of the slide glass. For this reason, after rotating the rotating shaft 15 for a predetermined time, the rotation of the rotating shaft 15 is stopped, the holder 9 is taken out from the centrifuge, the liquid remaining in the container cylinder 3 of the cell 1 is poured out, and the holding spring 12 is held. When the slide glass 8 is taken out and the solid glass to be observed with a microscope remains on the surface of the slide glass.
セル１の基板２とスライドガラス８との間に介在させる
パッキング板７は、遠心分離時に固形分を含んだ被処理
液がスライドガラス８の外に流出しないようにするもの
で、前述の様に従来はゴム板が使用された。The packing plate 7 interposed between the substrate 2 of the cell 1 and the slide glass 8 prevents the liquid to be treated containing solids from flowing out of the slide glass 8 during centrifugation. Conventionally, a rubber plate has been used.
しかしながら、ゴム板であると液分も流出しないので、
スライドガラス８を取出すときに、予め液分は容器筒３
から流し出すけれども、残留する液分のため、スライド
ガラス面に塗抹された固形分の一部が流出して失なわれ
ることが多いので、たとえば濾紙をパッキング板として
使用することが多くある。濾紙を使用すると、遠心分離
中に被処理液の液分が濾紙を透過して押出されるので、
遠心分離を終ったときに残留する液分が少なくなり、固
形分が液分で流されて固形分の収率が悪くなることが防
げる。However, if it is a rubber plate, liquid will not flow out, so
When the slide glass 8 is taken out, the liquid content is previously stored
Although it is drained from the filter, a part of the solid matter smeared on the surface of the glass slide often flows out and is lost due to the remaining liquid content. For this reason, filter paper is often used as a packing plate. When a filter paper is used, the liquid content of the liquid to be treated permeates the filter paper and is extruded during centrifugation.
It is possible to prevent the residual liquid content from being reduced when the centrifugal separation is finished, and to prevent the solid content from being washed away by the liquid content and the yield of the solid content being deteriorated.
（考案が解決しようとする問題点） ところが、パッキング板７として濾紙を使用した場合、
次に述べる様な不都合を生じる。(Problems to be solved by the invention) However, when a filter paper is used as the packing plate 7,
The following inconvenience occurs.
即ち、被処理液をセル１に注入してすぐに回転軸１５を
駆動し、遠心分離作業を始めればよいが、関連作業の関
係や多数のセルを使用して遠心分離をする場合に、セル
１の容器筒３内への被処理液注入から遠心分離開始まで
に若干の時間がかかることがある。そうすると、被処理
液が固形分を伴なって濾紙に染み込んでしまい、遠心分
離を開始したときに分離捕集できる固形分が減ってしま
うという不都合が生じる。That is, it is sufficient to drive the rotary shaft 15 immediately after injecting the liquid to be treated into the cell 1 and start the centrifugal separation work. However, in the case of performing the centrifugal separation using the relationship of the related work or using a large number of cells, It may take some time from the injection of the liquid to be treated into the container cylinder 3 of No. 1 to the start of centrifugation. Then, the liquid to be treated permeates the filter paper together with the solid content, which causes a disadvantage that the solid content that can be separated and collected when the centrifugal separation is started decreases.
この様な不都合を防止する為に、本考案者は先に、第６
〜８図に示した様な遠心塗抹用セルを考案した（実願昭
６０−５２１２９号）。この先考案に係る遠心塗抹用セ
ルは、基板２の一側に容器筒３ａ（第７図の例）、３ｂ
（第８図の例）を結合し、この容器筒３ａ、３ｂの内側
に連続する孔４を基板２の他側に開口させると共に、容
器筒３ａ、３ｂの中間部分を、基板２の孔４の開口より
も下方に曲げて溜り部１６を形成し、この溜り部１６の
少なくとも基板側の下面を傾斜壁１７として成るもので
ある。In order to prevent such inconvenience, the present inventor first
A cell for centrifugal smearing as shown in FIGS. 8 to 8 was devised (Japanese Patent Application No. 60-52129). The centrifugal smearing cell according to the present invention has a container cylinder 3a (example of FIG. 7), 3b on one side of the substrate 2.
(Example of FIG. 8) is combined to open a hole 4 continuous to the inside of the container cylinders 3a and 3b to the other side of the substrate 2, and to connect the middle portion of the container cylinders 3a and 3b to the hole 4 of the substrate 2. The reservoir 16 is formed by bending it downward from the opening, and at least the lower surface of the reservoir 16 on the substrate side is formed as an inclined wall 17.
この様な先考案に係る遠心塗抹用セルを用いて被処理液
１８の遠心塗抹処理を行なおうとした場合、容器筒３に
注入された被処理液１８は、遠心塗抹装置の回転軸１５
が停止している間は、溜り部１６に溜ってスライドガラ
ス８に迄は到達せず、従って待機中にパッキング板であ
る濾紙に固形分が吸収されてしまうことがないが、回転
軸を駆動して遠心分離作業を開始すると、被処理液１８
は遠心力のため傾斜壁１７を乗越えて基板２側に進み、
スライドガラス面に到達して遠心分離が行なわれる。When an attempt is made to perform centrifugal smearing of the liquid to be treated 18 using the centrifugal smearing cell according to the above invention, the liquid to be treated 18 injected into the container cylinder 3 is the rotating shaft 15 of the centrifugal smearing device.
While it is stopped, it does not reach the slide glass 8 by accumulating in the reservoir 16 and therefore the filter paper, which is a packing plate, does not absorb solids during standby, but it drives the rotary shaft. Then, when the centrifugal separation work is started, the liquid to be treated 18
Moves over the inclined wall 17 to the substrate 2 side due to centrifugal force,
The slide glass surface is reached and centrifugation is performed.
しかしながら、上述の様な先考案に係る遠心塗抹用セル
は、溜り部１６を設ける分だけ第２図に示した従来のセ
ルに比べて大型になる為、従来のセルを回転させる為の
遠心塗抹機と同じ大きさの塗抹機を使用するならば、セ
ルの容量をその分小さくしなければならず、一度に処理
出来る処理液の量が少なくなってしまう。However, the centrifugal smearing cell according to the above-described prior art is larger in size than the conventional cell shown in FIG. 2 by the provision of the reservoir portion 16, and therefore the centrifugal smearing cell for rotating the conventional cell is used. If a smearing machine of the same size as the machine is used, the capacity of the cell must be reduced accordingly, and the amount of processing liquid that can be processed at one time will decrease.
本考案の遠心塗抹用濾紙は、第２図に示した様な従来か
らのセルを使用しても遠心塗抹開始前に被処理液が流失
しない様にするものである。The filter paper for centrifugal smearing of the present invention prevents the liquid to be treated from flowing off before the start of centrifugal smearing even if a conventional cell as shown in FIG. 2 is used.
ｂ．考案の構成 （問題点を解決するための手段） 本考案の遠心塗抹用濾紙は、従来の遠心塗抹用濾紙と同
様に、基板の一側に容器筒を結合しこの容器筒の内側に
連続する孔を基板の他側に開口させて成る遠心塗抹用セ
ルの基板と、スライドガラスとの間に挟持して使用する
もので、第１図に示す様に遠心塗抹用セルの基板２の孔
４（第３図参照）に整合する通孔１９を形成している。b. Configuration of the Invention (Means for Solving the Problems) The centrifugal smear filter paper of the present invention, like the conventional centrifugal smear filter paper, has a container cylinder connected to one side of the substrate and is continuous inside the container cylinder. It is used by sandwiching it between a slide glass and a substrate of a centrifugal smearing cell having holes formed on the other side of the substrate, and as shown in FIG. A through hole 19 is formed to match (see FIG. 3).
本考案の遠心塗抹用濾紙に於いては更に、上記遠心塗抹
用セルの基板に開口した孔に整合する位置に形成した通
孔の内周縁部にのみ、シリコンオイル或はテフロンの様
な撥水性の液体を染み込ませている。In the centrifugal smear filter paper of the present invention, the water repellent material such as silicone oil or Teflon is formed only on the inner peripheral edge of the through hole formed at a position aligned with the hole opened in the substrate of the centrifugal smear cell. Is soaked with liquid.
（作用） 上述の様に孔４の内周縁部に撥水性の液体を染み込ませ
た本考案の遠心塗抹用濾紙は、従来のパッキング板７と
同様第３図に示す様に、セル１の基板２とスライドガラ
ス８との間に挟持して使用されるが、容器筒３に注入さ
れ孔４を通じて濾紙の通孔１９の内側に迄達した被処理
液は、この通孔１９の内周縁部に染み込んだ撥水性の液
体と水との間に作用する強い表面張力の影響で弾かれ、
待機中にパッキング板である濾紙に固形分が吸収されて
しまうことがない。(Function) The centrifugal smear filter paper of the present invention in which the inner peripheral edge of the hole 4 is impregnated with the water-repellent liquid as described above is the same as the conventional packing plate 7 as shown in FIG. The liquid to be treated, which is sandwiched between the slide glass 8 and the slide glass 8 and has reached the inside of the through hole 19 of the filter paper through the hole 4 through the hole 4, is the inner peripheral edge of the through hole 19. It is repelled due to the strong surface tension that acts between water and the water-repellent liquid
The solid content will not be absorbed by the filter paper, which is the packing plate, during standby.
遠心分離を開始すると、被処理液中の液分は、遠心力に
伴って発生する大きい液圧（通常使用される遠心分離器
の場合で２〜３kg/cm2）の為、上記表面張力に打ち勝っ
て濾紙の内側に染み込み、容器筒３の外に排出される。
又、処理液中の固形分は遠心力の為、スライドガラスの
表面に付着する。When the centrifugal separation is started, the liquid content in the liquid to be treated has a large liquid pressure ( 2 to 3 kg / cm 2 in the case of a commonly used centrifuge) generated by centrifugal force. It overcomes and permeates the inside of the filter paper, and is discharged to the outside of the container tube 3.
Further, the solid content in the treatment liquid adheres to the surface of the slide glass due to centrifugal force.
ｃ．考案の効果 本考案の遠心塗抹用濾紙を使用すれば、遠心塗抹用セル
の容器筒３内に注入した被処理液が遠心分離の待機中に
パッキング板７として使用する濾紙に吸収されて固形分
捕捉の効率を悪くする不都合をなくすことが出来るだけ
でなく、従来からの容量の大きいセルを使用して、一度
に多量の被処理液を処理する事が出来る。c. Effect of the Invention When the centrifugal smearing filter paper of the present invention is used, the liquid to be treated injected into the container cylinder 3 of the centrifugal smearing cell is absorbed by the filter paper used as the packing plate 7 while waiting for centrifugation, and the solid content is Not only can the inconvenience of deteriorating the efficiency of capture be eliminated, but a large amount of liquid to be treated can be treated at once by using a conventional cell having a large capacity.
第１図は遠心塗抹用濾紙の斜視図、第２図は従来のセル
を示す斜視図、第３図はセルをホルダに取付ける状況を
示す縦断面図、第４図はホルダの斜視図、第５図は遠心
分離機の略縦断面図、第６図は先考案に係る遠心塗抹用
セルの第１例を示す斜視図、第７図は第６図のＡ−Ａ断
面図、第８図は先考案の第２例を示す第７図同様の断面
図である。 １……セル、２……基板、３、３ａ、３ｂ……容器筒、
４……孔、５、６……補強リブ、７……パッキング板、
８……スライドガラス、９……ホルダ、１０……孔、１
１……折立縁、１２……抑えばね、１３……フック、１
４……遠心分離機、１５……回転軸、１６……溜り部、
１７……傾斜壁、１８……被処理液、１９……通孔。FIG. 1 is a perspective view of a filter paper for centrifugal smearing, FIG. 2 is a perspective view showing a conventional cell, FIG. 3 is a vertical cross-sectional view showing a situation in which the cell is attached to a holder, and FIG. 4 is a perspective view of the holder. 5 is a schematic vertical sectional view of the centrifuge, FIG. 6 is a perspective view showing a first example of the centrifugal smearing cell according to the prior invention, and FIG. 7 is a sectional view taken along the line AA of FIG. 6, and FIG. FIG. 7 is a sectional view similar to FIG. 7 showing a second example of the prior invention. 1 ... cell, 2 ... substrate, 3, 3a, 3b ... container cylinder,
4 ... hole, 5,6 ... reinforcing rib, 7 ... packing plate,
8 ... Slide glass, 9 ... Holder, 10 ... Hole, 1
1 ... Folding edge, 12 ... Pressing spring, 13 ... Hook, 1
4 ... Centrifuge, 15 ... Rotating shaft, 16 ... Reservoir,
17: inclined wall, 18: liquid to be treated, 19: through hole.
Claims (1)
の内側に連続する孔を基板の他側に開口させて成る遠心
塗抹用セルの基板とスライドガラスとの間に挟持する遠
心塗抹用濾紙であって、上記遠心塗抹用セルの基板に開
口した孔に整合する位置に形成した通孔の内周縁部にの
み、撥水性の液体を染み込ませた事を特徴とする遠心塗
抹用濾紙。1. A centrifuge smear cell having a container cylinder connected to one side of a substrate and having a continuous hole inside the container cylinder opened on the other side of the substrate and sandwiched between the substrate and a slide glass. A centrifugal smear filter paper, characterized in that a water-repellent liquid is impregnated only into the inner peripheral edge portion of a through hole formed at a position aligned with a hole opened in the substrate of the centrifugal smear cell. Filter paper.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1985193538U JPH0610281Y2 (en) | 1985-12-18 | 1985-12-18 | Centrifugal smear filter paper |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1985193538U JPH0610281Y2 (en) | 1985-12-18 | 1985-12-18 | Centrifugal smear filter paper |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62102145U JPS62102145U (en) | 1987-06-29 |
| JPH0610281Y2 true JPH0610281Y2 (en) | 1994-03-16 |
Family
ID=31149651
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1985193538U Expired - Lifetime JPH0610281Y2 (en) | 1985-12-18 | 1985-12-18 | Centrifugal smear filter paper |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0610281Y2 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2019078691A (en) * | 2017-10-26 | 2019-05-23 | サクラ精機株式会社 | Centrifugal smear specimen container holder |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0047840B1 (en) * | 1980-09-15 | 1985-03-06 | Shandon Southern Products Limited | Cytocentrifuge |
-
1985
- 1985-12-18 JP JP1985193538U patent/JPH0610281Y2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPS62102145U (en) | 1987-06-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP3060239B2 (en) | Cell centrifuge instrument, device and method | |
| CA1295817C (en) | Centrifugable pipette tip and pipette therefor | |
| US7758816B2 (en) | Large area cytocentrifuge sample chamber | |
| DE3334655C2 (en) | Centrifuge rotor | |
| JPH0610281Y2 (en) | Centrifugal smear filter paper | |
| US20020139743A1 (en) | Biological filtration station | |
| US4607570A (en) | Compression extractor | |
| CN213956809U (en) | Material evidence sticking device | |
| JPH0516519Y2 (en) | ||
| CN219615807U (en) | Centrifugal machine convenient to ejection of compact | |
| JPH0521003Y2 (en) | ||
| JPH05843Y2 (en) | ||
| CN208146226U (en) | A kind of chemical reagent centrifugation apparatus | |
| JPH0641153Y2 (en) | Cell holder for centrifugal smear | |
| JPH0521002Y2 (en) | ||
| JPH0540432Y2 (en) | ||
| CN2740272Y (en) | Dialysis container | |
| JPH0521004Y2 (en) | ||
| JPH0630275Y2 (en) | Filter filter for centrifuge | |
| JPH0436433Y2 (en) | ||
| JPS62176560A (en) | Filter for centrifuge | |
| CN218609896U (en) | Centrifuge tube shaking device for material-liquid separation | |
| CN213792241U (en) | Centrifuge convenient to wash | |
| CN223491164U (en) | Directional discharging mechanism of centrifugal machine | |
| CN216678488U (en) | Traditional chinese medicine draws uses centrifuge |