JPH0448531Y2 - - Google Patents

Description

[Detailed Description of the Invention] (Industrial Application Field) The present invention relates to a high-performance liquid chromatography apparatus, and more particularly to a technique for degassing a carrier liquid.

(Prior Art) A high-performance liquid chromatography apparatus uses a high-pressure pump to flow a liquid such as a solvent into a column as a carrier liquid, and a detector detects the separated components flowing out of the column.

By the way, the fluid resistance of the column is extremely large, so the pressure of the carrier liquid gradually decreases as it approaches the outlet of the column, and if there is a gas component dissolved in the carrier liquid, this gas will elute and create bubbles. occurs. These bubbles have problems in that they adhere to the stationary phase of the column and reduce the resolution, or they flow into the detector and cause baseline fluctuations.

Conventionally, in order to solve this problem, a decompression chamber was installed between the carrier liquid tank and the high-pressure pump to forcefully elute the dissolved gas, or the carrier liquid tank was bubbled with helium gas, which has extremely low solubility. Measures such as discharging dissolved gas or applying pressure to the detector to prevent pressure drop in the carrier liquid and suppress the generation of bubbles have been taken, but the first method The second method uses expensive helium, which increases running costs, and the third method requires a detector with low pressure resistance. There were problems such as the device being unusable and the cost of the device increasing.

(Purpose) The object of the present invention is to provide a novel high-performance liquid chromatography device that can reliably and inexpensively solve all of these conventional problems at once.

(Summary of the invention) In other words, the present invention is characterized by: a base having an ultrasonic transducer fixed to the back surface and housing a rotating magnetic field generating mechanism for generating a rotating magnetic field on the front surface;
A pedestal capable of containing a liquid on the surface of the pedestal and having a built-in means for heating the liquid, the pedestal having a carrier liquid tank accommodating a magnetic stirrer therein and communicating with a pressure reducing means. The bottom of the carrier liquid tank and the suction port of the carrier liquid feeding pump are connected by a pipe, thereby making it possible to degas, homogenize, and keep the temperature of the exchanged carrier liquid.

(Example) Therefore, the details of the present invention will be explained below based on the illustrated example.

The figure shows an embodiment of the present invention, in which reference numeral 1 denotes a cylindrical body 1a surrounding which a heater 2 is housed.
The lower end of the board is liquid-tightly attached to the substrate 1b, and an ultrasonic transducer 4 that receives power from the high-frequency oscillator 3 is fixed to the back surface of the substrate 1b.
A magnet 6 rotated by an electric motor 5 is disposed to face the magnet 6, and the magnet 6 is housed in a base 7 with the substrate 1b facing upward. 8 is a carrier liquid tank containing a carrier liquid, which houses a magnetic stirrer 9 inside and has a sealing stopper 8 that can be opened and closed.
The upper space can be selectively connected to the atmosphere opening 10 and the diaphragm vacuum pump 11 via the switching valve 12 through the a, and the bottom space is connected to the inlet of the high pressure pump 14 of the high performance liquid chromatography device 13. A connecting carrier liquid delivery pipe 15 is extended. Note that numerals 16 and 17 in the figure indicate a sample injection port and an analytical column, respectively.

In this embodiment, after opening the seal plug 8a and injecting a predetermined carrier liquid C into the liquid tank 8 so as to leave a space above, the seal plug 8a is closed again and placed on the pedestal 1, and the pedestal 1 is filled with water. Inject liquid L such as oil or oil.

After completing such preparations, the switching valve 12 is switched to the vacuum pump 11 side to operate the vacuum pump 11, and at the same time, the heater 2, high frequency oscillator 3, and motor 5 are operated. As a result, the pressure in the upper space S of the tank 8 is reduced by the vacuum pump 11, and the temperature of the carrier liquid C increases as it receives heat from the heater 2, and the solubility of gases such as oxygen dissolved in the carrier liquid C increases. descend. At the same time, carrier fluid C is
Vigorously shaken by stirring by the stirrer 9 and receiving elastic vibration from the ultrasonic vibrator 4 via the liquid L,
Evacuation of dissolved gases is promoted.

As a result, the temperature, pressure, and vibration act synergistically on the gas dissolved in the carrier liquid, and the gas component is reliably removed from the carrier liquid C.

When the degassing of the carrier liquid C is completed, the operation of the apparatus is stopped and the switching valve 12 is connected to the atmosphere opening port 10. When the liquid sending pump 14 is operated to send the degassed carrier liquid C from the tank 8 to the column 17, the carrier liquid C undergoes a pressure drop at the column outlet side and the detector, but as mentioned above, the pressure decreases due to temperature, atmospheric pressure, and Since the chamber is sufficiently degassed by vibration, it does not generate bubbles and provides highly accurate analysis results.

In the above-mentioned embodiment, the heater is provided on the circumferential surface of the pedestal, but the same effect can be achieved even if the heater is provided on the substrate. It is clear that the same effect can be achieved.

(Effects) As explained above, in the present invention, an ultrasonic transducer is fixed to the back surface, and a base housing a rotating magnetic field generation mechanism that generates a rotating magnetic field on the surface, and a liquid on the surface of the base. and a pedestal having a built-in means for heating the liquid, the pedestal accommodating a magnetic stirrer therein and a carrier liquid tank communicated with the pressure reduction means, the bottom of the carrier liquid tank and the carrier liquid being connected to each other. Since the suction port of the liquid feed pump is connected with a pipe, not only can new carrier liquid be easily degassed by simply replacing the liquid tank containing the carrier liquid, but it can also be used with carrier liquids consisting of multiple components. This has the effect of making it possible to achieve homogenization, and furthermore, maintaining the temperature of the carrier liquid, which affects analysis accuracy, at a constant level.

[Brief explanation of the drawing]

The figure is a configuration diagram of an apparatus showing an embodiment of the present invention. 1... pedestal, 2... heater, 4... ultrasonic vibrator, 5... electric motor, 6... magnet, 7... base, 8
... Carrier liquid tank, 8a ... Seal plug, 9 ... Stirrer, 11 ... Vacuum pump.

Claims (1)

[Scope of utility model registration request] A base having an ultrasonic transducer fixed to the back surface and housing a rotating magnetic field generating mechanism for generating a rotating magnetic field on the surface thereof, a liquid capable of being accommodated on the surface of the base, and a built-in means for heating the liquid. a pedestal, the pedestal houses a carrier liquid tank that houses a magnetic stirrer therein and communicates with a pressure reducing means, and connects the bottom of the carrier liquid tank and the suction port of the carrier liquid feeding pump with a pipe. A high-performance liquid chromatography device connected to