JPH0452677Y2 - - Google Patents

Description

[Detailed Description of the Invention] (Industrial Application Field) The present invention relates to a sample liquid storage cell for analysis, particularly to a test liquid storage cell equipped with two or more sample liquid storage sections.

The test liquid storage cell according to the present invention is used to store specimens, etc. in clinical tests for analyzing biochemical substances in human body fluids.

(Prior Art) A clinical test that uses human body fluids such as serum, plasma, urine, etc., particularly serum or plasma, as a specimen and analyzes the specimen to measure the amounts of various substances including biochemical substances. is widely used to diagnose the degree and transition of the pathology of various diseases.

In this clinical testing method, when serum or plasma is used as a specimen, the specimen is collected in a test tube, a reagent according to the purpose of analysis is added, and the resulting solution is transferred to a measurement cell. The amount of the target substance is measured by measuring coloration, absorbance, turbidity, etc. Therefore, this type of test requires a test tube as a reaction container and a cell as a measurement container.

(Problems to be solved by the invention and purpose of the invention) When implementing the conventional method, the reaction is carried out in a test tube and the measurement is carried out in a cell, which is troublesome as the contents must be transferred. It was hot. In addition, reaction containers such as test tubes are made of quartz or glass and are used repeatedly, so there is a risk of them being damaged during cleaning, etc., and if they are contaminated, it will cause errors in the measured values, so they must be stored carefully. There was a problem with what was needed.

Therefore, the basic purpose of the present invention is to provide a disposable cell that serves as both a reaction container and a measurement container for a sample solution for analysis, thereby simplifying the operation, and The aim is to minimize the introduction of errors.

Furthermore, in the analysis of substances as a clinical test, the ketone body ratio (acetoacetic acid/3-hydroxybutyric acid)
There are cases where it is necessary to measure two or more types of substances, such as in the past, but if separate containers are used as reaction vessels and measurement vessels as in the past, especially when processing multiple samples, There was a possibility that errors could occur when taking the wrong sample or dispensing the reaction reagent.

Therefore, an additional object of the present invention is to have two or more types of sample liquid storage sections, each of which serves as a reaction container and a measurement container, which is useful in processing multiple samples. Another object of the present invention is to provide a sample liquid storage cell in which the possibility that a sample is mistakenly taken or a reagent other than a prescribed reagent is introduced when dispensing a reagent to a sample is reduced in detail.

(Means and effects for solving the problem and achieving the object) According to the present invention, the above problem can be solved by using an integral molded product made of transparent plastic and at least two test liquids arranged side by side. A sample liquid storage cell for analysis, comprising a storage section and one blank measurement section, and a mark for confirming the first sample liquid storage section on at least one of both side edges. The above objective is achieved.

The test liquid storage cell according to the present invention (hereinafter sometimes simply referred to as a "cell") is made of transparent plastic and has a relatively low cost, so it can be made into a so-called "disposable" cell.
The plastic material that is the constituent material of this cell is not attacked by specimens, reagents, and their reaction products.
It is necessary to select a material with good transmission characteristics in the ultraviolet region, and for example, acrylic resin materials, particularly polymethyl methacrylate resins, are preferred.

In the cell according to the present invention, the mark for confirming the first sample liquid storage area is provided because when measuring two or more substances, a sample such as serum is dispensed into each sample liquid storage area. In addition, predetermined and different reagents are dispensed into each sample liquid storage part, but in this case, the purpose is to ensure that the predetermined reagent is dispensed into the predetermined sample liquid storage part without error.
This mark may be a lug-shaped protrusion. The sample and reagent are reacted, and the absorbance, turbidity, etc. of this reaction solution are measured.In order to minimize scattering of the measurement light by the cell itself, the cross-sectional shape of the sample solution storage section is rectangular. is preferred. The dimensions of the sample liquid storage part are preferably relatively small so that measurements can be made with a small amount of specimen and reagent, for example, the internal dimensions are about 8 mm in width and 8 mm in depth, and the height is such that the light receiving part for photometry is as tall as the sample liquid storage part. However, from the viewpoint of handling the cell (for example, preventing contamination of the light receiving part for photometry by grasping the top of the cell) and cost, it should be around 40mm. is preferred. If the blank measurement section is configured in the same way as the sample liquid storage section, the air present inside will cause a lens effect and scatter the measurement light, so it is preferably configured as a space.

(Example) Next, an example of implementation of the cell according to the present invention will be described with reference to the drawings.

In the drawings, FIG. 1 is a perspective view of a sample liquid storage cell 10 for analysis according to the present invention, and FIG. 2 is a sectional view taken along the line -- in FIG.

This cell 10 is integrally molded from polymethyl methacrylate resin, which is an acrylic transparent resin material, and basically consists of two sample liquid storage parts 1.
2 and 14, and one blank measuring section 16. A lug-shaped protrusion 18 is formed on the outer surface of the first sample liquid storage section 12, so that it can be distinguished from the second sample liquid storage section 14 in appearance.

In the cell 10 according to this embodiment, the first sample liquid storage section 12 and the second sample liquid storage section 14 are separated by spacers 20a and 20b, and a space is formed below the spacer, and this space is used as a blank. It is made into the measurement part 16. When a sample such as serum is dispensed into the first and second sample liquid storage units 12 and 14, and then separate reagents are dispensed into each sample liquid storage unit (the order of dispensing serum and reagents is reversed). ), since the first sample liquid storage section 12 is formed with a lug-shaped protrusion 18 which is a mark for confirmation, and the first sample liquid storage section 12 and the second sample liquid storage section are 14 are separated by the spacers 20a and 20b, it is possible that an erroneous operation such as a reagent to be dispensed into one sample liquid storage section being dispensed into the other sample liquid storage section, or It is possible to prevent as much as possible the occurrence of an erroneous operation in which a reagent to be dispensed into the sample liquid storage section of the method is introduced into both sample liquid storage sections.
In addition, as long as the reagent can be reliably dispensed, it is not necessarily necessary that the first sample liquid storage section 12 and the second sample liquid storage section are arranged apart from each other; The sample liquid storage section, the second test liquid container section, and the blank measurement section are arranged in parallel in this order. Furthermore, since the blank measuring section is just a space as described above, the width of the lug-like protrusion 18, which is a confirmation mark of the first sample liquid storage section 12, is made slightly larger to fill the space below the protrusion 18. It can also be used as a blank measuring section.

In the cell 10 according to the present embodiment, the internal volumes of both sample liquid storage sections 12 and 14 are the same, and the internal dimensions are 8 x 8 x 40 mm.

(Example of Use) A case will be described below in which plasma is used as a specimen and the blood ketone body ratio is measured using the cell according to the present invention shown in the drawings and described in the Examples.

1 Preparation of specimen After adding heparin as an anticoagulant to the collected arterial blood, immediately centrifuge at 4°C and 3000 rpm for 10 minutes. Add the same amount of 6% perchloric acid solution to the plasma obtained.
After stirring for ~3 seconds, cool on ice for at least 10 minutes, and cool at 4℃.
Centrifuge at 3000 rpm for 10 minutes, collect the supernatant, and use it as a specimen (store until use; stable for 48 hours on ice, 3 days at -20°C, and 30 days at -70°C).

2 Reagent a Common oxygen test solution Prepared by mixing 1.7 ml of stock solution of 3-hydroxybutyrate dehydrogenase with 1.5 ml of purified water (if stored on ice,
(stable for days).

b Coenzyme test solution for measuring acetoacetic acid (A) Prepared by mixing 4 ml of triethanolamine with 1.2 mg of lyophilized β-NADH (stable for 10 days if stored on ice).

c Coenzyme test solution for measuring 3-hydroxybutyric acid (B) Prepared by mixing 40.4 mg of β-NAD ⁺ lyophilized product with 8 ml of Tris salt buffer (stable for 20 days if stored on ice).

3 Measuring operation a Dispense 0.25 ml of coenzyme test solution A into the first test solution storage section 12 of the cell 10, and also dispense 0.25 ml of coenzyme test solution A into the second test solution storage section 14.
Dispense 0.25 ml of coenzyme test solution B into the first and second
Place 0.5 of the sample in the sample liquid storage parts 12 and 14, respectively.
Dispense 1 ml, mix gently, and incubate at 37°C (5 minutes). Next, the first and second sample liquid storage sections 12, 14 and the blank measuring section 16 are photometered at a wavelength of 340 nm to measure the absorbance.

b Next, the first and second sample liquid storage sections 12 and 14
Dispense 0.05 ml of the enzyme test solution into each tube, mix gently, and incubate at 37℃ for 15 minutes. Thereafter, the absorbance is measured in the same manner as in section a above.

c The acetoacetic acid concentration, 3-hydroxybutyric acid concentration, and the ketone body ratio, which is the ratio thereof, can be determined by a well-known method based on the above-mentioned absorbance measurement values.

(Effects of the invention) The cell for storing a sample liquid for analysis according to the invention is made of plastic material and is relatively inexpensive, so it can be disposable. Since this cell is equipped with at least two sample liquid storage areas, two or more substances can be analyzed simultaneously by dispensing the same sample into these sample liquid storage areas and introducing separate reagents into each of these sample liquid storage areas. It can be used for.

For example, the so-called "ketone body ratio", that is, acetoacetic acid/3
-Hydroxybutyric acid ratio has traditionally been used as an index for clinical diagnosis of diabetes, and now it has become an important diagnostic index for failure of various organs including the liver.The cell according to the present invention is suitable for measurement in such cases. It is extremely suitable for use, and can effectively prevent incorrect collection of specimens and incorrect insertion of reagents.

[Brief explanation of the drawing]

FIG. 1 is a perspective view of a sample liquid storage cell according to the present invention for biochemical analysis, and FIG. 2 is a sectional view taken along the - line in FIG. DESCRIPTION OF SYMBOLS 10... Test liquid storage cell, 12, 14... Test solution storage section, 16... Blank measurement section, 18... Mark for checking the first test solution storage section 12 (lug-shaped protrusion), 20a, 20b...Spacer.

Claims (1)

[Claims for Utility Model Registration] (1) An integrally molded product made of transparent plastic, comprising at least two sample liquid storage sections and one blank measurement section arranged side by side,
A sample liquid storage cell for analysis, characterized in that a mark for confirming a first sample liquid storage section is provided on at least one of both side edges. (2) The sample liquid storage cell for analysis according to claim 1, wherein the mark for confirming the first sample liquid storage part is a lug-shaped protrusion. (3) The cross-sectional shape of the sample liquid storage section is rectangular and has the same dimensions, and there are two sample liquid storage sections separated by a spacer, and the lower part of both sample liquid storage sections is A cell for storing a sample liquid for analysis according to claim 1 or 2 of the utility model registration claim, characterized in that a space is formed between the cells and the space forms a blank measuring section. .