JPH0452732B2 - - Google Patents
Info
- Publication number
- JPH0452732B2 JPH0452732B2 JP1011380A JP1138089A JPH0452732B2 JP H0452732 B2 JPH0452732 B2 JP H0452732B2 JP 1011380 A JP1011380 A JP 1011380A JP 1138089 A JP1138089 A JP 1138089A JP H0452732 B2 JPH0452732 B2 JP H0452732B2
- Authority
- JP
- Japan
- Prior art keywords
- lighting
- days
- keito
- lux
- hours
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 241000196324 Embryophyta Species 0.000 claims description 21
- 239000003795 chemical substances by application Substances 0.000 claims description 13
- 238000012258 culturing Methods 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 241000758791 Juglandaceae Species 0.000 claims description 8
- 235000020234 walnut Nutrition 0.000 claims description 8
- HUTDUHSNJYTCAR-UHFFFAOYSA-N ancymidol Chemical compound C1=CC(OC)=CC=C1C(O)(C=1C=NC=NC=1)C1CC1 HUTDUHSNJYTCAR-UHFFFAOYSA-N 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 5
- 239000000049 pigment Substances 0.000 claims description 5
- 239000004615 ingredient Substances 0.000 claims description 4
- 238000005286 illumination Methods 0.000 description 7
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 7
- 239000002609 medium Substances 0.000 description 6
- 239000006870 ms-medium Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 239000000654 additive Substances 0.000 description 4
- 241000218922 Magnoliophyta Species 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 2
- 241000234435 Lilium Species 0.000 description 2
- 241000960400 Torenia Species 0.000 description 2
- 241000722921 Tulipa gesneriana Species 0.000 description 2
- 238000011109 contamination Methods 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 2
- 229960001669 kinetin Drugs 0.000 description 2
- 235000010746 mayonnaise Nutrition 0.000 description 2
- 239000008268 mayonnaise Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 244000015329 Aeginetia indica Species 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 241000219317 Amaranthaceae Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000201841 Celosia Species 0.000 description 1
- 240000004713 Pisum sativum Species 0.000 description 1
- 235000010582 Pisum sativum Nutrition 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 244000184734 Pyrus japonica Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 235000008954 quail grass Nutrition 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 230000003381 solubilizing effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000008400 supply water Substances 0.000 description 1
Landscapes
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、矮化けいとうの栽培方法に関するも
のである。更に詳述に述べれば、密閉容器中で矮
化けいとうを栽培して開花させ、そのまま長期間
にわたる観賞が可能な矮化けいとうの栽培方法に
関するものである。DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention relates to a method for cultivating dwarf walnuts. More specifically, the present invention relates to a method for cultivating dwarf walnuts that allows dwarf walnuts to be cultivated in a closed container, bloomed, and then be admired for a long period of time.
密閉容器中で矮化けいとうを栽培して開花さ
せ、そのまま観賞可能とする矮化けいとうの栽培
方法は未開発である。
A method for cultivating dwarf keito in which the dwarf keito is grown in an airtight container, bloomed, and can be admired as is has not yet been developed.
そもそも、密閉容器中で草花を栽培し、開花さ
せることは大変困難であり、矮化トレニアについ
て成功した報告（特開昭60−221020号公報）があ
るのみである。この矮化トレニアも、今日迄商品
化されるに至つていない。現在、密閉容器中で矮
化植物を育成し、観賞用に供されているものは、
花をつけない観葉植物ばかりである。 In the first place, it is very difficult to cultivate and flower flowers in a closed container, and there is only one successful report on dwarf Torenia (Japanese Patent Laid-Open No. 60-221020). This dwarf Torenia has also not been commercialized to date. Currently, dwarf plants are grown in airtight containers and used for ornamental purposes.
They are all ornamental plants that do not produce flowers.
本発明者も、多くの種類の草花について、密閉
容器中で矮化させ、しかも開花させる条件を矮化
剤の種類、矮化剤の濃度、照明等の栽培条件を中
心に研究してきたが、容易には開花に成功しなか
つた。しかしながら、今般、けいとうに関して、
密閉容器中で矮化させ、しかも開花させるための
新知見をようやく得、ここに本発明を完成した。
The present inventor has also studied the conditions for dwarfing and flowering many types of flowers in airtight containers, focusing on the type of dwarfing agent, the concentration of the dwarfing agent, and cultivation conditions such as lighting. It did not easily succeed in flowering. However, regarding Keito,
We have finally obtained new knowledge on how to dwarf and bloom in a closed container, and have now completed the present invention.
即ち、本発明は次の２発明である。 That is, the present invention consists of the following two inventions.
第１番目の発明は、透視可能な容器中に入れた
農林水産省登録第13980号に係る矮化剤（成分：
アンシミドール〔α−シクロプロピル−α（４−
メトキシフエニル）−５−ピリミジンメタノール〕
0.025％、水、色素等99.975％、性状：緑色可溶
化液体）２〜10ml／添加剤のムラシゲスクーグ
培地にけいとうの無菌幼植物体の全草を植えつ
け、しかる後、照度：2000〜3000ルツクス、照明
時間：８〜10時間／１日、照明期間：30〜50日間
の照明条件下で無菌的に密閉培養することを特徴
とする矮化けいとうの栽培方法である。 The first invention is a dwarfing agent (ingredients:
Ancimidol [α-cyclopropyl-α(4-
methoxyphenyl)-5-pyrimidine methanol]
0.025%, water, pigment, etc. 99.975%, properties: green solubilized liquid) 2 to 10 ml/additives of a sterile young plant of Kyoto was planted in Murashigeskoog medium, and after that, the illuminance: 2000 to 3000 lux. This is a method for cultivating dwarf walnuts, which is characterized by aseptically closed culture under lighting conditions of 8 to 10 hours/day of lighting and 30 to 50 days of lighting period.
第２番目の発明は、透視可能な容器内に入れた
農林水産省登録第13980号に係る矮化剤（成分：
アンシミドール〔α−シクロプロピル−α（４−
メトキシフエニル）−５−ピリミジンメタノール〕
0.025％、水、色素等99.975％、性状：緑色可溶
化液体）２〜10ml／添加済のムラシゲスクーグ
培地にけいとうの無菌幼植物体の全草を植えつけ
照度：2000〜3000ルツクス、照明時間：８〜10時
間／１日、照明期間：30〜50日間の照明条件下で
無菌的に密閉培養して花序を形成したけいとうを
得、次いで、前記花序を切断し、前記と同種の矮
化剤１〜10ml／添加のムラシゲスクーグ培地に
前記切断花序を植えつけ、しかる後、照度：2000
〜3000ルツクス、照明時間：８〜10時間／１日、
照明期間：７〜10日間の照明条件下で無菌的に密
閉培養することを特徴とする矮化けいとうの栽培
方法である。 The second invention is a dwarfing agent (ingredients:
Ancimidol [α-cyclopropyl-α(4-
methoxyphenyl)-5-pyrimidine methanol]
0.025%, water, pigment, etc. 99.975%, properties: green solubilized liquid) 2 to 10 ml/added whole plant of sterile seedlings of Kyoto was planted in Murashigeskoog medium, illuminance: 2000 to 3000 lux, lighting time: 8 to 10 hours/day, lighting period: 30 to 50 days, aseptically and closed culture to obtain inflorescence-forming inflorescences, and then cut the inflorescences to dwarf the same species as above. The cut inflorescences were planted in a Murashigeskoog medium containing 1 to 10 ml of the agent, and then the illumination intensity was set to 2000.
~3000 lux, lighting time: 8-10 hours/day,
Illumination period: This is a method for cultivating dwarf walnuts, which is characterized by aseptically closed culturing under illumination conditions for 7 to 10 days.
本発明における「けいとう」は、やりけいと
う、久留米けいとう、さきわけけいとう等のヒユ
科ケイトウ属植物の総称である。そして、けいと
うは、「茎は直立して条線があり、赤みを帯び、
高さ50〜90cmになる。葉は互生し、卵形あるいは
披針形で長さ５〜10cm、先がとがる。秋、茎頂に
赤、紅、黄、白などの小花を密集してつけ、その
上緑部が鶏冠状に帯化する。」（小学館発行：国語
大辞典）ものである。 In the present invention, "Keito" is a general term for plants of the genus Celosia in the Amaranthaceae family, such as Yarikeito, Kurume Keito, and Sakiwake Keito. And Keito says, ``The stem is upright and striated, reddish,
It grows to a height of 50-90cm. The leaves are alternate, ovate or lanceolate, 5-10 cm long, and have a pointed tip. In autumn, small red, crimson, yellow, and white flowers are densely produced at the top of the stem, and the green part forms a crest-shaped band on top. ” (published by Shogakukan: Japanese Dictionary).
本発明では、農林水産省登録第13980号に係る
矮化剤（成分：アンシミドール〔α−シクロプロ
ピル−α（４−メトキシフエニル）−５−ピリミジ
ンメタノール〕0.025％、水、色素等99.975％、
性状：緑色可溶化液体）を使用する。この矮化剤
は、「スリトーン」（イーライリリー社の登録商標
−以下同じ）の名称で市販（製造：武田薬品工業
株式会社、販売：武田園芸資材株式会社）されて
いる。スリトーンは、節間の伸長を抑制して植物
の草丈を低くする新しい矮化剤で、きく、ゆり、
チユーリツプ等の草花、球根及び花木類中でも鉢
物の草花・花木用矮化剤である。スリトーンの説
明書には、用いる植物の種類や品種によつて微妙
な効果の差があると記載されているが、密閉容器
中で上記のきく、ゆり、チユーリツプを矮化させ
且つ開花させることは本発明者の幅広い条件設定
による実験でも不可であり、唯一「けいとう」に
顕著な効果が認められた。 In the present invention, the dwarfing agent according to Ministry of Agriculture, Forestry and Fisheries Registration No. 13980 (components: ancymidol [α-cyclopropyl-α(4-methoxyphenyl)-5-pyrimidine methanol] 0.025%, water, pigment, etc. 99.975%) %,
Properties: Green solubilizing liquid) is used. This dwarfing agent is commercially available under the name "Sritone" (registered trademark of Eli Lilly and Co., same hereinafter) (manufactured by Takeda Pharmaceutical Co., Ltd., sold by Takeda Horticultural Materials Co., Ltd.). Suritone is a new dwarfing agent that reduces the height of plants by suppressing the elongation of internodes.
It is a dwarfing agent for flowering plants such as tulips, bulbs, and flowering trees, especially potted plants and flowering trees. The instructions for Slitone state that there are subtle differences in effectiveness depending on the type and variety of plants used, but it is not possible to dwarf and bloom the above-mentioned lilies, lilies, and tulips in an airtight container. Even in experiments conducted by the present inventor under a wide range of conditions, this was not possible, and only "Keito" was found to have a remarkable effect.
けいとうの全草を密閉培養するに当たつては、
スリトーンをムラシゲスクーグ培地（以下、
「MS培地」という）中に２〜10ml／添加して
用いる。スリトーンの添加量が、２ml／未満で
は矮化効果が不十分であり、10ml／を越えると
薬害により、けいとうが枯死する率が高くなる。 When culturing the entire Keito plant in a closed culture,
Suritone in Murashigeskoog medium (hereinafter referred to as
It is used by adding 2 to 10 ml/ml into ``MS medium'' (referred to as "MS medium"). If the amount of Sulitone added is less than 2 ml/ml, the dwarfing effect will be insufficient, and if it exceeds 10 ml/ml, the rate of death of Kyoto will increase due to chemical damage.
一方、けいとうの花序部分のみを切断し、この
花序部分を密閉培養するに当たつては、スリトー
ンをMS培地中に１〜10ml／添加して用いる。
スリトーンの添加量が、１ml／未満では矮化効
果が不十分であり、10ml／を越えると薬害によ
り、けいとうが枯死する率が高くなる。 On the other hand, when cutting only the inflorescence part of Kyoto and cultivating this inflorescence part in a sealed manner, 1 to 10 ml of Sulitone is added to the MS medium.
If the amount of Sulitone added is less than 1 ml/ml, the dwarfing effect will be insufficient, and if it exceeds 10 ml/ml, the rate of death of the Kyoto will increase due to chemical damage.
なお、30℃以上の高温条件下（夏季）でけいと
うを培養する場合には、スリトーンの添加量が５
ml／未満では生長が早く、観賞期間が短くなる
ので、５ml／以上とすべきである。 In addition, when culturing Keito under high temperature conditions of 30℃ or higher (summer), the amount of Sulitone added should be 5.
If the amount is less than 5 ml/ml, the growth will be rapid and the viewing period will be shortened, so the amount should be 5 ml/or more.
培養に当たつては光を照射するが、その照度
は、約2000〜3000ルツクスの範囲が好適である。
2000ルツクス未満では生長が十分に行われないの
で培養期間がいたずらに長くなり、3000ルツクス
を越える照明は過剰である。また、照明時間は、
１日（24時間）の内、約８〜10時間とするのが好
適である。８時間／１日未満では生長が十分に行
われないので培養期間がいたずらに長くなり10時
間／１日を越えると生長が促進される結果、けい
とうが徒長して観賞に耐えられなくなる。照明期
間（培養期間）は、けいとうの全草を密閉培養す
るに当たつては約30〜50日間の範囲、けいとうの
花序部分のみを切断し、この花序部分を密閉培養
するに当たつては約７〜10日間の範囲が好適であ
る。 During cultivation, light is irradiated, and the illumination intensity is preferably in the range of approximately 2000 to 3000 lux.
If the illumination is less than 2,000 lux, the cultivation period will be unnecessarily long as insufficient growth will occur, and if the illumination exceeds 3,000 lux, it will be excessive. In addition, the lighting time is
Approximately 8 to 10 hours per day (24 hours) is preferred. If the culturing period is less than 8 hours/day, sufficient growth will not occur, and if the culture period exceeds 10 hours/day, the growth will be accelerated, and as a result, the plants will become overgrown and unfit for ornamental viewing. The lighting period (cultivation period) is approximately 30 to 50 days when culturing the whole plant of Keito in a sealed manner, or approximately 30 to 50 days when only the inflorescence part of the Keito is cut and this inflorescence is cultured in a sealed manner. A range of about 7 to 10 days is suitable.
前記各照明期間よりも短いと植物体が十分に生
長せず、通常の室内に放置するときに枯死する確
率が高くなる。一方、前記照明期間よりも長い
と、いたずらに培養期間を長びかせるだけで不経
済であるとともに、培養中に花序が開花してしま
うので、出荷後の観賞期間が短くなる。 If the lighting period is shorter than each of the above-mentioned lighting periods, the plant will not grow sufficiently and will have a high probability of dying when left indoors. On the other hand, if it is longer than the above-mentioned illumination period, the cultivation period will be unnecessarily prolonged, which is uneconomical, and the inflorescences will bloom during cultivation, resulting in a shortened viewing period after shipment.
本発明に係る矮化けいとうの栽培方法は、透視
可能な密閉容器中で行われる。透視可能な容器と
しては、三角フラスコ、試験管、瓶等を挙げるこ
とができ、これから任意に選択使用すればよい。 The method for cultivating dwarf potato according to the present invention is carried out in a see-through sealed container. Examples of containers that can be seen through include Erlenmeyer flasks, test tubes, and bottles, and any one of them may be used.
また、本発明に係る矮化けいとうの栽培方法
は、無菌的培養でなければならない。さもなけれ
ば、植物体はカビ、ウイルス、細菌に侵されて枯
死する。 Furthermore, the method for cultivating dwarf peas according to the present invention must be aseptic culturing. Otherwise, the plant will be attacked by mold, viruses, and bacteria and die.
以下に本発明の代表的な実施例を示し、更に本
発明の詳細を説明する。
Below, typical examples of the present invention will be shown and further details of the present invention will be explained.
実施例 １
（やりけいとうの全草を利用する場合）
やりけいとうの種子２ｇを200ml三角フラスコ
に入れ、ツイーン（Tween）20を0.1％添加した
滅菌水を注ぎ、アンチホルミンを10ml加えて撹拌
後放置した。10分後に種子を滅菌水で２回洗浄
し、水を切つた後70％エタノールを200ml入れて
放置した。10分後に添加物を含まない滅菌水で２
回洗浄し、無菌種子とした。Example 1 (When using the whole plant of Yarikeito) Place 2 g of Yarikeito seeds in a 200ml Erlenmeyer flask, pour in sterile water containing 0.1% Tween 20, add 10ml of antiformin, and stir. I left it behind. After 10 minutes, the seeds were washed twice with sterilized water, drained, and left in 200 ml of 70% ethanol. After 10 minutes, rinse with sterile water without additives.
The seeds were washed twice and made into sterile seeds.
上記の無菌種子を、寒天0.9％のMS培地を入れ
た試験管に10粒ずつ置床し、25℃暗条件で放置し
た。１週間後に汚染率及び発芽率を調査した結
果、汚染率は試験管の本数レベルで30％、発芽率
は個体レベルで45％であつた。汚染されていない
発芽分の試験管を25℃の室内において、照度：
3000ルツクス、照明時間：10時間／１日、照明期
間：２週間の照明条件下で培養し、無菌幼植物体
を得た。 Ten of the above sterile seeds were placed in test tubes containing MS medium containing 0.9% agar and left in the dark at 25°C. As a result of investigating the contamination rate and germination rate one week later, the contamination rate was 30% at the number of test tubes level, and the germination rate was 45% at the individual level. Place the uncontaminated germinated test tubes in a room at 25°C and adjust the illuminance:
The plants were cultured under lighting conditions of 3000 lux, lighting time: 10 hours/day, lighting period: 2 weeks, and sterile seedlings were obtained.
このようにして得た無菌幼植物体の中から茎直
径0.5mmのもの30本を選び出し、200mlの規格瓶
（通称マヨネーズ瓶）10個に入れられたスリトー
ン６ml／添加剤のMS培地に前記無菌幼植物体
の全草３本ずつを植え付け、耐熱性の透明プラス
チツク製の蓋をして、25℃の室内において、照
度：3000ルツクス、照明期間：10時間／１日、照
明期間：50日間の照明条件下で培養した。植え付
け後30日間で全ての個体に花序が形成され、50日
間で観賞に耐える花序が形成された。植付け時
に、平均して、茎直径0.5mm、茎高さ２cm、花序
直径0.2mm、花序長さ0.5cmであつたものが、60日
経過後では、平均して、茎直径は1.0mm、茎高さ
５cm、花序直径１cm、花序長さ２cmであつた。 From the sterile seedlings thus obtained, 30 stems with a diameter of 0.5 mm were selected and placed in MS medium containing 6 ml of Slitone/additives in 10 200 ml standard bottles (commonly known as mayonnaise bottles). Plant three whole seedlings each, cover with a heat-resistant transparent plastic lid, and store in a room at 25°C, illuminance: 3000 lux, lighting period: 10 hours/day, lighting period: 50 days. Cultured under light conditions. Inflorescences were formed in all individuals 30 days after planting, and ornamental inflorescences were formed in 50 days. At the time of planting, the average stem diameter was 0.5 mm, the stem height was 2 cm, the inflorescence diameter was 0.2 mm, and the inflorescence length was 0.5 cm, but after 60 days, the average stem diameter was 1.0 mm, and the stem height was 1.0 mm. The height was 5 cm, the inflorescence diameter was 1 cm, and the inflorescence length was 2 cm.
規格瓶10個のうち５個については引続き同一照
明条件下で培養し、残りの５個については直射日
光の当らない窓際に放置して培養した結果、共に
５ケ月以上に亘つて花序を形成し続け、開花した
植物体を生体のまま維持させることができた。な
お、この間水分及び栄養分の供給は１回も行なわ
なかつた。 Five of the 10 standard bottles were continued to be cultured under the same lighting conditions, and the remaining five were left to grow by a window out of direct sunlight. As a result, both of them formed inflorescences for over 5 months. As a result, they were able to maintain the flowering plant as a living organism. Note that during this period, water and nutrients were not supplied even once.
なお、別の実験において、MS培地に0.1〜1.0
％のカイネチン（ホルモン）を添加しておくと、
花芽の形成数が多くなり、さらに観賞価値が高く
なることを確認した。 In addition, in another experiment, 0.1 to 1.0
If you add % of kinetin (hormone),
It was confirmed that the number of flower buds formed increased and the ornamental value was further increased.
実施例 ２
（やりけいとうの花序部分を利用する場合）
実施例１と同様にして作出したやりけいとうの
無菌幼植物体の中から茎直径約0.5mmもの30本を
選び出し、450mlの規格瓶（通称マヨネーズ瓶）
10個に入れられたスリトーン６ml／を添加した
MS培地に前記無菌幼植物体の全草３本ずつを植
え付け、耐熱性の透明プラスチツク製の蓋をし
て、25℃の室内において、照度：3000ルツクス、
照明時間：10時間／１日、照明期間：40日間の照
明条件下で培養して、花序が形成されたやりけい
とうを得た。Example 2 (When using the inflorescence part of A. japonica) 30 stems with a diameter of approximately 0.5 mm were selected from the sterile seedlings of a. (commonly known as mayonnaise bottle)
Added 6 ml of Slitone in 10 pieces
Three whole plants of the above-mentioned sterile seedlings were planted on MS medium, covered with a heat-resistant transparent plastic lid, and placed in a room at 25°C under illuminance: 3000 lux.
Cultivation was carried out under lighting conditions of lighting time: 10 hours/day and lighting period: 40 days to obtain buds with inflorescences formed.
次いで、このやりけいとうの花序部分のみを切
断（茎の長さは約１cm）した。200ml／の規格
瓶10個に入れられたスリトーン６ml／添加剤の
MS培地に、切断した花序部分３個ずつを植え付
け、耐熱性透明プラスチツク製の蓋をして、25℃
の室内において、照度：3000ルツクス、照明時
間：10時間／１日、照明期間：１週間間の照明条
件下で培養を続けた。１週間で花序は培地に挿入
している茎の切断部分から発根し、地上部分は
次々と花序を形成し続け、葉がない状態で生長を
続け、60日間では平均して花序直径３cm、花序高
さ1.5cmとなつた。 Next, only the inflorescence part of this japonica was cut (the length of the stem was about 1 cm). 6ml/additives of Slitone in 10 200ml/standard bottles.
Plant 3 cut inflorescence parts each in MS medium, cover with heat-resistant transparent plastic lid, and hold at 25°C.
Cultivation was continued in a room under the following lighting conditions: illuminance: 3000 lux, lighting time: 10 hours/day, lighting period: 1 week. In one week, the inflorescences will root from the cut part of the stem inserted into the medium, and the above-ground part will continue to form inflorescences one after another, continuing to grow without leaves, and in 60 days, the inflorescences will have an average diameter of 3 cm. Inflorescence height is 1.5cm.
規格瓶10個のうち５個については引続き同一照
明条件下で培養し、残りの５個については直射日
光の当らない窓際に放置して培養した結果、共に
４ケ月以上に亘つて花序を形成し続け、開花した
植物体を生体のまま維持した。 Five of the 10 standard bottles were continued to be cultured under the same lighting conditions, and the remaining five were cultured by leaving them near a window out of direct sunlight. As a result, both of them formed inflorescences for over 4 months. The flowering plants were then maintained as living organisms.
なお、別の実験において、MS培地に0.1〜1.0
％のカイネチン（ホルモン）を添加しておくと花
序の分岐及び側芽の形成数が多くなり、さらに観
賞価値が高くなることを確認した。 In addition, in another experiment, 0.1 to 1.0
% of kinetin (hormone) increases the number of branching of inflorescences and the formation of lateral buds, further increasing the ornamental value.
更に補足説明をすると、久留米けいとう、さき
わけけいとうも上記実施例１、２と同一条件で栽
培できる。 As a supplementary explanation, Kurume keito and Sakiwake keito can also be cultivated under the same conditions as in Examples 1 and 2 above.
本発明は、密閉容器中に特定の矮化剤を入れ、
照度：2000〜3000ルツクス、照明時間：８〜10時
間／１日、照明期間：けいとうの全草を密閉培養
する場合には30〜50日間、けいとうの花序部分の
みを切断し、この花序部分を密閉培養する場合に
は７〜10日間という特定の証明条件下でけいとう
を栽培するので、けいとうを徒長させることなく
開花させ、そのままの状態を長期間にわたつて保
持することができる。
The present invention involves placing a specific dwarfing agent in a sealed container,
Illuminance: 2,000-3,000 lux, lighting time: 8-10 hours/day, lighting period: When culturing the whole plant in a closed culture, cut only the inflorescence part of the plant and grow it for 30-50 days. When culturing the parts in a closed culture, the keito is cultivated under specific conditions for 7 to 10 days, so the keito can bloom without elongating and can maintain that state for a long period of time. .
本発明は以上説明した通りの矮化けいとうの栽
培方法である。この栽培方法によつて初めて得ら
れた密閉容器中の開花した矮化けいとうは、４ケ
月以上に亘つて開花状態を維持し続け、その間、
水分及び栄養分の供給は全く必要なく、容器が小
さいことからテーブル、棚等容器を置くスペース
さえあればいかなる場所にも飾ることができ、矮
化けいとうが開花しているというめずらしさが加
わり、商品化の十分可能な産業利用価値の高いも
のである。
The present invention is a method for cultivating dwarf walnuts as explained above. The flowering dwarf keito in a sealed container, which was obtained for the first time using this cultivation method, maintained its flowering state for more than four months, and during that time,
There is no need to supply water or nutrients at all, and since the container is small, it can be displayed on a table, shelf, or anywhere else as long as there is space for the container.It also adds the rarity of a flowering dwarf keito, making it a great product. It is highly valuable for industrial use as it can be fully developed.
Claims (1)
13980号に係る矮化剤（成分：アンシミドール
〔α−シクロプロピル−α（４−メトキシフエニ
ル）−５−ピリミジンメタノール〕0.025％、水、
色素等99.975％、性状：緑色可溶化液体）２〜10
ml／添加済のムラシゲスクーグ培地にけいとう
の無菌幼植物体の全草を植えつけ、しかる後、照
度：2000〜3000ルツクス、照明時間：８〜10時
間／１日、照明期間：30〜50日間の照明条件下で
無菌的に密閉培養することを特徴とする矮化けい
とうの栽培方法。 ２ 透視可能な容器内に入れた農林水産省登録第
13980号に係る矮化剤（成分：アンシミドール
〔α−シクロプロピル−α（４−メトキシフエニ
ル）−５−ピリミジンメタノール〕0.025％、水、
色素等99.975％、性状：緑色可溶化液体）２〜10
ml／添加済のムラシゲスクーグ培地にけいとう
の無菌幼植物体の全草を植えつけ、照度：2000〜
3000ルツクス、照明時間：８〜10時間／１日、照
明期間：30〜50日間の照明条件下で密閉培養して
花序を形成したけいとうを得、次いで、前記花序
を切断し、前記と同種の矮化剤１〜10ml／添加
のムラシゲスクーグ培地に前記切断花序を植えつ
け、しかる後、照度：2000〜3000ルツクス、照明
時間：８〜10時間／１日、照明期間：７〜10日間
の照明条件下で無菌的に密閉培養することを特徴
とする矮化けいとうの栽培方法。[Scope of Claims] 1. The Ministry of Agriculture, Forestry and Fisheries registered No.
Dwarfing agent according to No. 13980 (Ingredients: Ancimidol [α-cyclopropyl-α(4-methoxyphenyl)-5-pyrimidinemethanol] 0.025%, water,
99.975% pigment, properties: green solubilized liquid) 2-10
mL/ml/added Murashigeskoog medium is planted with sterile seedlings of Keito, and after that, illuminance: 2000 to 3000 lux, lighting time: 8 to 10 hours/day, lighting period: 30 to 50 days. A method for cultivating dwarf walnuts, which is characterized by aseptically closed culturing under lighting conditions. 2 Ministry of Agriculture, Forestry and Fisheries registration number placed in a see-through container
Dwarfing agent according to No. 13980 (Ingredients: Ancimidol [α-cyclopropyl-α(4-methoxyphenyl)-5-pyrimidinemethanol] 0.025%, water,
99.975% pigment, properties: green solubilized liquid) 2-10
ml/ml/added Murashigeskoog medium with whole plants of sterile seedlings of Keito, illuminance: 2000~
3000 lux, lighting time: 8 to 10 hours/day, lighting period: 30 to 50 days to obtain the inflorescence-forming inflorescences. The cut inflorescences were planted in Murashigeskoog medium supplemented with 1 to 10 ml of a dwarfing agent, and then exposed to illuminance: 2000 to 3000 lux, lighting time: 8 to 10 hours/day, lighting period: 7 to 10 days. A method for cultivating dwarf walnuts characterized by culturing them under aseptic closed conditions.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1011380A JPH02190113A (en) | 1989-01-19 | 1989-01-19 | Method for cultivating dwarfed feather cockscomb |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1011380A JPH02190113A (en) | 1989-01-19 | 1989-01-19 | Method for cultivating dwarfed feather cockscomb |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH02190113A JPH02190113A (en) | 1990-07-26 |
| JPH0452732B2 true JPH0452732B2 (en) | 1992-08-24 |
Family
ID=11776407
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1011380A Granted JPH02190113A (en) | 1989-01-19 | 1989-01-19 | Method for cultivating dwarfed feather cockscomb |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH02190113A (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103535278B (en) * | 2013-09-27 | 2015-01-14 | 华中农业大学 | Production method of cockscomb tube flower |
| CN104303794A (en) * | 2014-10-29 | 2015-01-28 | 陈卫香 | Cultivation method for cockscomb |
| CN104823834B (en) * | 2015-04-23 | 2018-01-26 | 湖北大学 | The Method of Breeding New Cockscomb Varieties Using Epigenetic Principles and Distant Hybridization |
| CN105794628B (en) * | 2016-03-18 | 2017-11-17 | 湖北大学 | It is a kind of to utilize chimera cockscomb and the method for feather cockscomb selection cross feather cockscomb kind |
| CN106688843A (en) * | 2016-11-22 | 2017-05-24 | 蚌埠市涂山绿园蔬菜科研专业合作社 | Soilless planting method of cockscomb |
-
1989
- 1989-01-19 JP JP1011380A patent/JPH02190113A/en active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPH02190113A (en) | 1990-07-26 |
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