JPH0454101A - Preservation of kidney for implantation and preserving device of same kidney - Google Patents

Abstract

PURPOSE:To preserve kidney for implantation in an aqueous solution by making a preserving device comprising a water tank receiving an aqueous solution of divalent or trivalent iron salt and an automatic filter circulating said solution in the tank and filtering, connecting renal artery, etc., of extracted kidney to the filter and washing inside of the kidney. CONSTITUTION:In a preserving device composed of a water tank 1, an automatic filter 2 and an electromagnetically vibrating diaphragm pump 3, a suction port 4 of the pump is connected to renal artery 6 of an extracted kidney 10 through a hose 5 and renal veins 7 and ureter 8 are opened in a solution 11. The pump is driven and residual material in the extracted kidney 10 is sucked out, then the space after removed off the residual material is filled with the aqueous solution 11. The solution containing the residual material is risen in a way expressed by arrows and filtered in a filtering chamber 12, then flowed down into the water tank 1. The aqueous solution contains divalent or trivalent iron salt. Said divalent or trivalent iron salt is inorganic salt or organic salt of iron exhibiting a property intermediate between divalent iron and trivalent iron. Thereby, the extracted kidney is not decomposed because of retardation of ionic reaction and continues a hibernation state with keeping bionic functions.

Description

DETAILED DESCRIPTION OF THE INVENTION <Industrial Application Field> The present invention relates to a method and apparatus for preserving a kidney for transplantation.

(Prior Art) It is thought that more than 90% of dialysis patients who are forced to undergo artificial dialysis due to kidney disease desire kidney transplantation.

However, the kidney bank does not have a single kidney stored in it, making it presumptuous to call it a bank, and one can imagine that it is not very effective. The biggest obstacle is that organs cannot be preserved for a long time. When it comes to kidneys, it is said that cadaveric kidneys can be used, but it goes without saying that it is still preferable to transplant the kidney within a few hours after death.On the other hand, there are several conditions for transplantation, such as blood type, age, size, presence of disease, etc. Considering that there are few people willing to donate their kidneys, it must be said that there is little hope for patients.

Kidney transplants in Europe and America are relatively often done with cadaveric kidneys, but if the kidney is removed within 30 minutes after breathing and circulation stop, washed with cooled perfusate, and kept at a low temperature, it can be stored for 1 to 2 days. It is said that it is now possible to transplant the system. It seems that 1 to 2 days is the limit of the shelf life.

<Problems to be Solved by the Invention> If the storage period of cadaveric kidneys becomes sufficiently long, kidneys of various blood types can be preserved in kidney punctures due to the goodwill of donors, and can be transplanted to suitable patients at any time.

The inventor explored this possibility.

Once a biological tissue is separated from a biological solid, biological components such as proteins and carbohydrates immediately begin to decompose because the biological system is destroyed and tissue functions are lost. This means that the biological system was maintained by some substance supplied from the biological solid. Therefore, if some of these substances can be removed from the human body, the kidneys (as well as other organs) should be able to maintain their biological functions for a long period of time after being removed from the human body. In particular, the kidney has a structure that allows substances to be easily supplied to the entire tissue from the outside.

In this way, there must be some kind of substance that maintains the biological functions of the living body, and this is the key to preserving the removed kidney.

I formulated this hypothesis and aimed to realize it.

〈Means for solving the problem〉 Even though science has advanced, human research on biological systems is still in its infancy, and I did not think that any substance that maintains biological functions had already been elucidated. Anyway, I researched the literature extensively. To their surprise, the substance had already been discovered. The substances are divalent and trivalent iron salts, which will be described later.

The method for preserving a kidney for transplantation of the present invention thus developed is characterized by washing the inside of the removed kidney using an aqueous solution of a divalent or trivalent iron salt as a perfusate, and preserving the kidney by immersing it in the aqueous solution.

The apparatus for preserving a kidney for transplantation of the present invention comprises a water tank containing an aqueous solution of a divalent or trivalent iron salt, and an automatic filtration device that circulates and filters the liquid in the tank. Then, the whole removed kidney is soaked and dripped into the bath liquid from the filtering device, and one of the renal vein, ureter and renal artery of the kidney is connected to the suction or discharge port, and the other is soaked in the bath liquid. It is characterized by having an opening inside so that the liquid can continue to circulate.

<Action> Divalent and trivalent iron salts control various ionic reactions. It functions to maintain biological functions.

If the inside of the removed kidney is washed with an aqueous solution of divalent or trivalent iron salts as a perfusate, divalent or trivalent iron salts will be present in the renal arteries, veins, renal corpuscles at the tips of the ureters, and even the renal tubules. It permeates through the body and prevents the decomposition of biological components through its ionic reaction inhibiting action, preserving biological functions. According to the preservation method of the present invention, in which the kidney is immersed in an aqueous solution of the same divalent or trivalent iron salt in this state, the removed kidney can be preserved in a hibernating state for a long period of time.

Further, the storage device of the present invention serves both as a device for washing the inside of a removed kidney and as a storage device for the washed removed kidney. For washing, submerge the removed kidney in a bath containing an aqueous solution of divalent or trivalent iron salts, connect one side of its renal vein, ureter, and renal artery to the pump side, and submerge the other side in the solution in the bath. When the tank is opened and placed in the circulation path of the filtration device, the axilla in the tank goes around every corner of the inside of the excised kidney.
After being filtered in the filtration chamber, it returns to the original tank. If blood, urine, etc. still remain inside the removed kidney, the filtered fluid may be discarded outside the tank and new fluid may be replenished into the tank.

When using this as a storage device, leave the filtration device turned off.
Alternatively, you can keep your kidneys in hibernation by simply circulating the fluid periodically. Replacement and cleaning of the material in the filtration chamber is only necessary at first.

Examples) The divalent and trivalent iron salts used in this invention and their production method are disclosed in JP-A-59-190226, and are the result of 30 years of research by Professor Shoji Yamashita of the Faculty of Agriculture, Nagoya University. It is.

The divalent and trivalent iron salts include inorganic salts such as iron hydrochloride, sulfate, phosphate, and nitrate, which have intermediate properties between divalent iron and trivalent iron, as well as formate, acetate, and propionate. Organic salts such as trivalent iron salts are added to a large amount of strong alkali aqueous solution such as caustic soda, potassium hydroxide, lithium hydroxide, calcium hydroxide, etc. to convert the valence of trivalent iron to divalent iron. It is obtained as a transition form when a salt of divalent iron is introduced into a large amount of an aqueous solution of a strong acid such as hydrochloric acid or sulfuric acid to cause valence conversion to trivalent iron.

The specifications of the published publication include 1. Corrosion prevention of metals, 2. Removal of salt damage, 3. Improvement of soil with continuous cropping damage, 4. tissue preservation,
5. Regeneration of plant tissues, 6. Abiotic synthesis of biological components, 7.
Specific examples of use such as antiseptic, antifungal action, 8. antiviral action, 9. anticancer action, etc. are shown.
4. From the requirements of ``Biological Tissue Preservation,'' as mentioned above, I learned that this is a substance that preserves the biological functions of living organisms.

In this example, immediately after killing a white rat, the muscle tissue was placed in a bottle, a treatment solution was added thereto, the bottle was sealed tightly and left at room temperature, leaving a partial room air layer. At the same time, as a control, distilled water was added to the muscle tissue and the tissue was sealed and left standing.

As a result, the tissue in the control plot collapsed after one week, microorganisms multiplied, and water rapidly dried up. However, the tissue of the samples from the treated area remained intact, microorganisms did not propagate, and the liquid remained clear for more than 12 years.

The above treatment solution is a 10-6M solution of iron chloride (■, ■) with an IQm
(L contains a-tocopherol and Ubiquin
One (Co-enzyme Q7) 0.1 g of each mixture was added and suspended, the above lipid portion was collected with ethanal, and Tween-20,
Add 0.1g and disperse in water, then dilute with distilled water sequentially.
The adjusted solution had a lipid concentration of 2 x 10-12 M/L.

This invention does not simply preserve a removed kidney, but also removes the kidney from the human body, suspends all its various activities within the body, and during that time, removes and cleans the inside to remove divalent and trivalent substances. Since iron salts permeate through the body and suppress ionic reactions, the aforementioned properties are exerted, so it is quite conceivable that this is not just a period of hibernation, but a period of time for the improvement of one tissue and the establishment of a system for suppressing bacteria, viruses, and cancer cells. .

Once it became possible to safely hibernate a kidney that had been removed in this way, one kidney from a patient could be removed and hibernated in order to treat kidney disease, and therapeutic drugs depending on the disease could be added to an aqueous solution of divalent or trivalent iron salts. In addition, it will also be possible to directly increase the therapeutic effect and then return it to the patient's body.

Next, an embodiment of the storage device of the present invention will be described.

The figure is a schematic diagram of an embodiment in which an aquarium fish tank with an automatic filtration device is modified into a storage device for a kidney for transplantation. In this example, an automatic filtration device 2 is placed on top of the water tank 1. In this example, the suction port 4 of the electromagnetic oscillating diaphragm pump 3 is lowered to near the bottom of the tank, and is connected to the renal artery 6 of the excised kidney 10 via a hose 5. The cut end of the renal vein 7 and the ureter 8 of the excised kidney 10 open into the fluid 11.

The liquid 11 is an aqueous solution of divalent or trivalent iron salt, and it is preferable to add a thrombolytic agent initially to ensure complete internal cleaning.

When the pump motor insert 3a is connected to a power source and started, the suction port 4 becomes negative pressure, and the residue inside the excised kidney 10 is sucked out from the renal vein 7 and ureter 8.

After that, the aqueous solution 11 flowing in from the renal artery 6
The liquid 11 containing the suctioned residue, remaining liquid, etc. is filled and sequentially heads toward the suction port 4, rising as shown by the arrow and being filtered while descending through the filtering chamber 12 of the filtration device 2.

The liquid flowing out from the hole in the bottom of the filtration chamber 12 flows down from the liquid reservoir 13 to the water tank l through the outlet pipe 14 as shown by the arrow. Alternatively, a hose may be attached to the lower end of the outlet pipe 14 to discharge the water out of the tank.

In the above embodiment, the overchamber 12 is simple, probably because a ready-made product is used, but it is planned to be made larger and installed outside the tank, and research on furnace materials will be conducted in the future.

Other ideas include adding an ultrasonic vibration device to apply ultrasonic waves only while cleaning the inside of the removed kidney, occasionally reversing the flow, changing the speed, and changing the temperature of the liquid.

This device also allows the divalent and trivalent iron salt aqueous solution inside the excised stomach to be replaced with physiological saline.

<Effects of the Invention> This invention greatly improves the delay in kidney preservation techniques, which required kidney transplantation to be performed within 1 to 2 days at the latest after removal.

As a doctor of engineering with kidney disease, the present inventor is acutely aware of the need for techniques for preserving kidneys that are currently ineffective. He hypothesized that there must be some kind of substance, and that if the substance was supplied and covered, the removed kidney could be preserved without losing its function. With this invention, we have been able to prove its validity.

That is, through research and investigation, the present inventor recognized that the divalent and trivalent iron salt aqueous solution developed by Professor Sanno of Nagoya University corresponds to the above hypothetical substance, and developed this invention in which this is used as an internal cleaning solution and soaking solution for a removed kidney. We were able to provide basic knowledge on kidney removal techniques.

The removed kidney, which has been internally cleaned and soaked in an aqueous solution of divalent and trivalent iron salts, can decompose because the ionic reaction is suppressed.
It continues to hibernate while maintaining its biological functions. Therefore, when it is returned to the human body, it receives oxygen and energy from the influx of blood, wakes up, and begins its original function. During its hibernation, or storage, it was possible to prevent decomposition by suppressing ionic reactions.

In addition, the transplant kidney preservation device of the present invention automatically continues soaking the excised kidney in a divalent or trivalent iron salt aqueous solution, permeating and circulating the aqueous solution into the kidney, and filtering it. Immediately after the kidney is removed, it is placed in this storage device to automatically clean the inside of the kidney, and after that, the kidney can be soaked in the tank for long-term storage, or drugs can be added to the solution in the tank to improve the therapeutic effect. You can also give

This invention made it possible to store a large number of removed kidneys of various types in cases of kidney failure, put the data into a computer, and put them on standby, and opened a new way to temporarily remove and treat diseased kidneys. It brings much good news to fellow believers.

[Brief explanation of drawings]

The figure is an explanatory view of one embodiment of the kidney transplantation preservation device of the present invention, in which 1 is a water tank and 2 is an automatic filtration device.

Claims (2)

[Claims] (1) A method for preserving a kidney for transplantation, which comprises washing the inside of the removed kidney using an aqueous solution of a divalent or trivalent iron salt as a perfusate, and preserving the kidney by immersing it in the aqueous solution. (2) It consists of a water tank filled with an aqueous solution of divalent or trivalent iron salts and an automatic filtration device that circulates and filters the solution in the tank, and soaks the whole excised kidney in the solution in the tank, One of the renal veins, ureters and renal arteries of the kidney is connected to the suction or discharge port hanging down from the filtration device into the tank liquid, and the other is opened into the tank liquid to circulate the liquid. A storage device for a kidney for transplantation, characterized in that the storage device continues to carry out the following steps.