JPH049313A - Cosmetic - Google Patents

Abstract

PURPOSE:To provide a safe cosmetic having stable whitening activity, antiinflammatory activity and water-retainability by compounding the water-soluble extract of the fruit of Lagenaria leucantha Rusby ver. ciauat Makino. CONSTITUTION:A cosmetic contains the water-soluble extract of the fruit of Lagenaria leucantha Rusby ver. ciauat Makino. For example, the fruit may be extracted with one or a mixture of solvents comprising alcohols such as water, methanol, ethanol and 1,3-butylene glycol, acetone, etc., by a heating extraction method or an ordinary temperature extraction method. The extract is compounded in a solid amount of 0.01-10wt.%, preferably 0.1-5.0wt.%, based on the total amount of the cosmetic.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention relates to a novel cosmetic. More specifically, the present invention relates to a cosmetic product that contains a water-soluble extract of the fruit of Pigeon as an active ingredient and has a whitening effect, an anti-inflammatory effect, and a water-retaining ability.

[Conventional technology] Skin problems Although there are some unknowns about the mechanism by which freckles occur, in general, melanin pigments are formed due to hormonal abnormalities or stimulation by ultraviolet rays from sunlight. It is thought that it is abnormally deposited in To treat such dark spots and freckles, substances that inhibit tyrosinase activity in the skin and suppress melanin production, such as large doses of vitamin C, or glutathione in ointments, creams, and lotions, etc. Methods such as making it into a form and applying it locally are used. Additionally, hydroquinone preparations are used as pharmaceuticals in Europe and America.

In addition, various skin irritations, such as the action of ultraviolet rays, cause skin inflammation, and anti-inflammatory agents suppress these inflammations.As ingredients for cosmetics, aloe vera,
Plant extracts such as loofah, glycyrrhizin, zinc oxide, etc. are used.

In addition, when moisture is reduced from the stratum corneum of the skin, it causes rough skin. In order to provide an appropriate moisture content to the stratum corneum, humectants such as glycerin, 1,3-boo-IF-L/glycol, propylene glycol, and hyaluronic acid are used.

Note 5: The common usage of Yugao is mainly in the production of Kanpyo in the food field, but there is no record of its use as a cosmetic.

[Problems to be Solved by the Invention] Vitamin C has poor stability against light over time and causes discoloration and odor, especially in systems containing water. On the other hand, the use of hydroquinone is restricted due to safety issues such as skin irritation and allergy. In addition, it is susceptible to air oxidation, which poses a problem in terms of stability. Thiol compounds such as glutathione and cysteine have a strong odor, are easily oxidized, and have slow effects. Also, 2-
Mercaptoethylamine salt, N-(2-mercaptoethyl)dimethylamine salt, and the like are known to bleach the skin of black guinea pigs, but they are not generally used because vitiligo tends to occur after bleaching.

On the other hand, there are various ingredients that have whitening, anti-inflammatory, and moisturizing effects (as mentioned above), but in order to produce cosmetics that have all of these effects, it is necessary to add each of these ingredients. However, there are many points that must be kept in mind, such as product stability.

[Means for trying to solve the problem] The present inventors
In view of this situation, as a result of extensive research, it was discovered that a cosmetic containing all the active ingredients of the water-soluble extract of Yugao fruit exhibits good whitening effect, anti-inflammatory effect, and water retention ability, and the present invention has been developed. It was completed.

The present invention is a cosmetic that contains a water-soluble extract of the fruit of Pigeon as an active ingredient.

The eucalyptus used in the present invention is a member of the Cucurbitaceae family, Lagenaria euca, and its scientific name is Lagenaria 1euca.
n-1ha Ru5by ver, ciauat M
The cited document called akino is the primary color Makino plant encyclopedia:
For example, cultivated products can be used.

The fruit of the plant of the present invention is extracted with a water-soluble solvent, and examples thereof include solvents such as water, alcohols (methacrylate, ethanol, 13-butylene gelicol, propylene glycol, etc.), and acetone. It may be extracted using one type or a mixed solvent of two or more of these water-soluble solvents. Further, it may be extracted by heating or extracted at room temperature. If necessary, it can be concentrated or diluted and used as a cosmetic raw material.

The cosmetics of the present invention may contain oils and fats, waxy hydrocarbons, fatty acids, alcohols, esters, metallic soaps, within the range that does not impair the effects of the water-soluble extract of the fruit of the lily.
Surfactants and the like can be blended as raw materials.

Cosmetics manufactured using these base materials as raw materials include:
Examples include cosmetic creams, milky lotions, shampoo foundations, lip balms, and lipsticks.

The amount of the water-soluble extract of Yugao fruit used in the cosmetic composition of the present invention is 0.01 to 10% by weight based on the total cosmetic composition, preferably 0.01 to 10% by weight as the solid content obtained by distilling off the solvent.
It is appropriate to add 01 to 5.0% by weight. 0,0
If it is less than 1% by weight, no sufficient effect can be expected, and if it exceeds 10% by weight, the effect will not be enhanced and it is uneconomical.

Further, the method of addition may be either added in advance or added during production, and may be appropriately selected in consideration of workability.

The present invention will be described in more detail with reference to Examples below, but the present invention is not limited thereto. show.

[Example Example 1 500g of Yugao fruit was shredded and mixed with 500ml of water.
1 g of extract (containing 99% or more solids) was obtained by heating and extracting twice at different times and further concentrating by vacuum freeze-drying.

Example 2 After thoroughly washing 100 g of Kanbyo (commercial product) with water,
The mixture was heated and extracted twice with 1000 ml of water for 2 hours each time. After the residue was filtered off, the filtrate was concentrated under reduced pressure and further freeze-dried in vacuo to obtain 6 g of extract (containing 99% or more solids).

Example 3 60 g of dried Yugao fruit was crushed, heated and extracted with 600 ml of a water-ethanol mixture (1:1) for 5 hours, and further concentrated to obtain 2 g of extract (containing 50% solids). Tokuasahi Example 4 60 g of dried Yugao fruit was crushed, 300 ml of ethanol was added, and the mixture was left at room temperature for 1 month.

By further concentrating, 1 g of extract (containing more than 99% solids) was obtained.Asahi Example 5 60 g of dried Psyllium fruit was crushed and heat extracted twice with 600 ml of propatool for 2 hours each. 1 g of extract (contains 70% solids) by further concentration
I got it.

Example 6 Toner ■Hot water extract of Yugao fruit (99% as solids) 5.0 parts■Glycerin 2.0■Ethyl alcohol 7.0■Methyl roseoxybenzoate 0.05■Polyoxyethylene ( 20) Lauryl ether 0.5 ■ Citric acid 0. OI■Sodium citrate 0.1■Purified ice
85.34 [Production method] Mix and dissolve ingredients ① to ②. Separately, mix and dissolve ingredients ① to ②. The two are then mixed and filtered through Tetron cloth (300 mesh) to obtain a product.

Example 7 Toner ■Hot water extract of Citrus chinensis (99% as solid) ■Glycerin ■Ethyl alcohol ■Polyoxyethylene (20) lauryl ether ■Methyl paraoxybenzoate 2.0 parts 7.0 0.5 0 .05 ■Citric acid 0.01■
Sodium citrate 0.1■ Purified water 115.34 [Production method] Mix and dissolve ingredients (1) to (2). Separately, mix and dissolve ingredients ① to ②. Next, mix the two and use Tetron cloth (30
0 mesh) to obtain the product.

Example 8 Cream ■Hot water extract of Yugao fruit (99% as solids) ■Squalane ■Olive oil ■Stearic acid ■Beeswax ■Octyldodecyl myristate ■Polyoxyethylene (20) Cetyl ether ■Behenyl alcohol ■Glycerin monostearate 5.5 3.0 2.0 3.5 1.5 parts [phase] 1,3-butylene glycol 8.
5 ■ Methyl paraoxybenzoate 0.20 Ethyl paraoxybenzoate 0.050 Flavor
o, i@purified water
66.15 [Manufacturing method] Ingredients (1) to (2) are heated and dissolved, mixed, and kept at 70°C to form an oil phase. component[
[Phase] - @ is heated and dissolved in component 0, mixed, and kept at 75°C to form an aqueous phase. The aqueous phase is added to the oil phase to emulsify it, and the ingredients are added and cooled to 30°C while stirring to form a product.

Example 9 Emulsion ■Ethanol extract of Yugao fruit (99% as solid content) ■Squalane ■Olive oil ■Jojoba oil ■Cetyl alcohol ■Glycerin monostearate ■Polyoxyethylene (20) 1.0 5.0 5.0 5.0 1.5 2.0 Cetyl ether 3,0 ■Polyoxyethylene (20) Sorbitan monooleate 2.0 ■Dipropylene glycol i.

[Phase] Glycerin 2.00
Fragrance 0.10 Methyl paraoxybenzoate 0.20 Purified water
71.12 [Manufacturing method] Ingredients (1) to (2) are heated and dissolved, mixed, and kept at 70°C to form an oil phase. Ingredients■
, [Phase], @ are heated and dissolved in component @, mixed and kept at 75°C to form an aqueous phase. Add the aqueous phase to the oil phase to emulsify and disperse, add component (1), and cool to 30°C while stirring.

Example 1 O back ■ Propylene glycol extract of Yugao fruit (20% as solids) ■ Glycerin ■ 1,3-butylene glycol ■ Polyoxyethylene (40) Coal 3.0 parts 11.5 2.5 Hydrogenated castor oil 1,0 ■ Citric acid
O1 ■ Sodium citrate
0.3 ■ Methyl paraoxybenzoate
0.2 ■Fragrance
0.05 ■ Purified water 708
5 [Manufacturing method] Mix ingredients ① to ① uniformly and dissolve them to form a product.

[Effects of the Invention] The cosmetic composition of the present invention containing the water-soluble extract of Prunus nigra fruit as an active ingredient has a highly stable whitening effect, anti-inflammatory effect, and water-retaining ability, and is also preferable in terms of safety. It is.

Hereinafter, the present invention will be explained in detail by giving experimental examples.

[Experimental example] 11 (Soft) 1. In order to investigate the whitening effect and tyrosinase activity inhibition effect, 0.1 of the sample was
The tyrosinase activity inhibitory power of the 5% aqueous solution was measured before and after incubation at 37°C for 2 weeks.

For comparison, ascorbic acid, loofah water, and a hot water extract of loofah fruit, which have been conventionally used as cosmetics, were similarly tested. Also, hot water extract of Chima (for comparison)
As a preparation method, dry product iog is extracted with hot water (95%
'C, 3 hours, 300 ml), the filtrate was vacuum freeze-dried and the tyrosinase activity inhibitory effect was measured; In a test tube, 1 ml of L-tyrosine solution (0.3 m/+cl) was added to Max Payne's buffer ( 1 ml of 0.15% aqueous solution of the sample (0.9 cod) was added, and the mixture was incubated in a thermostatic water bath at 37° C. for 10 minutes. To this was added 0.1+nl of tyrosinase aqueous solution (1 mH/ml), stirred well, and incubated at 37'C for 12 minutes, then set in a spectrophotometer and measured absorbance at 475 nm.

On the other hand, similar absorbance measurements were performed using distilled water instead of the sample as a blank, and the tyrosinase activity inhibition rate of each sample was calculated using the following formula. In addition, A in the formula means the absorbance when each sample is added, and B means the absorbance of the blank.

Inhibition rate (%) = (1-A/B)X100 The results of these tests are shown in Table 1. From this table, it can be seen that the water-soluble extract of Yugao fruit obtained in Example 1 has a more remarkable ability to inhibit tyrosinase activity than the loofah water and the hot water extract of loofah. Good stability, 37℃, 2
After being left for a week, it was found to have a stronger tyrosinase activity inhibiting ability than vitamin C. In addition, these stability tests revealed that the water-soluble extract of the fruit of Yugao showed no odor or discoloration. Furthermore, when the water-soluble extracts of Yugao fruits obtained in Examples 2 to 5 were similarly tested, they were found to exhibit equally good tyrosinase activity inhibition ability.

A histamine release inhibition test was conducted using an aqueous solution F with a concentration of 0.01, 01.10% for each sample. For comparison, loofah water and loofah hot water extract, which have been conventionally used in cosmetics, were similarly tested. The water-soluble extract of lilypodium, the loofah water, and the hot water extract of loofah are the same as those used in Experimental Example 1.

Histamine release inhibition test; report by Hirai et al. (Herbal medicine miscellaneous!
37. 374.1983. ) according to male 5p
raque-D Ley rat (200-450K
) was measured for its inhibitory effect on histamine release on superior sulcus cells collected from the abdominal cavity. That is, the effect of suppressing histamine release by 4 ppm of compound 48/80 was determined as release inhibition rate (%). The results are shown in Table 2. From these results, it was found that the hot water extract of the fruit of Aloe albopictus obtained in Example 1 did not have a remarkable effect of suppressing histamine release and had an excellent anti-inflammatory effect, compared to the hot water extract of Aloe aloe. I found it.

Furthermore, when the water-soluble extracts of Yugao fruit obtained in Examples 2 to 5 were similarly tested, they were found to exhibit good anti-inflammatory effects.

The test was conducted in the same manner using distilled water as a liquid sample. The hot water extract obtained in Example 1 was used as the sample. Asahi method for measuring moisture evaporation inhibition rate; 2 ml of each sample solution was placed in a measuring bottle at a temperature of 25".
C. Leave at relative humidity of 50%.

After standing still for 4 days, the amount of water evaporation of each sample solution was measured, and the water evaporation rate was calculated using the following formula. In addition, A in the formula means the amount of water evaporation of each sample, and B means the amount of water evaporation of the blank (distilled water).

Inhibition rate (%) = (1-A/B)xlo.

The test results are summarized in Table 3. As is clear from the results, it was found that the hot water extract obtained in Example 1 of the present invention had a moisture evaporation inhibition rate comparable to that of glycerin and was excellent in water retention capacity.

Table 1. Tyrosinase activity inhibition effect 111 Suhu 1 Yu Moisturizing effect The moisture evaporation inhibition rate was measured for the 0.5% and 7.0% aqueous solutions of the samples. For comparison, glycerin, which has traditionally been used as a moisturizing agent in cosmetics, was compared with Bravitamin CO,
159526 Luffa water 0.15 Luffa 0.15 Hot water extract Table 2. Histamine release inhibitory effect Example 1 1.0 0.1 0, Ol Luffa water 1.0 0.1 Kidachi aloe 1.0 Hot water extract 0.1 0.01 Table 3. Moisture evaporation inhibition rate Example 1 0.5 5.0? ,0
31.7 Glycerin 0.5 5.0? ,0
32.8 1 Blood Determination X Person 11 Usage Test Usage testing was conducted using 30 healthy subjects.

As samples, the cosmetics of Examples 6 and 7 were used, and the weight percentages of the water-soluble extracts of P. elegans and P. elegans were varied. A 20TIl square site on the inner side of the subject's forearm was irradiated with ultraviolet light at an intensity of 3 mW/Cm2 for 1 minute using a tlV-B lamp (Toshiba PL-203E). After applying each sample twice a day in the morning and evening for three days on each site, we conducted a questionnaire survey to evaluate the inflammation suppressing effect.We also conducted a questionnaire survey regarding the pigmentation suppressing effect and skin moisturizing effect after one month of use. A survey was conducted and an evaluation was conducted.One site that was irradiated with ultraviolet rays was used as a control without any application.9 Evaluations were made by comparing with the control based on the criteria for the questionnaire.

(Judgment criteria) Effective ◎ Somewhat effective ○ Mostly ineffective Δ Ineffective It can be seen that the material has an effect of suppressing inflammation and pigmentation after a significant sunburn, and also shows a good effect on the moist feeling of the skin.

The results confirmed that the water-soluble extract of Yugao has extremely low primary irritation and is highly safe for the skin. In addition, the water-soluble extracts of Yugao fruit obtained in Examples 2 to 5 were similarly tested and found to be similarly highly safe for the skin.

Patent applicant Nonoyo Shoji Ltd. 1 Summer iX Member Emperor Safety Test

Claims (1)

[Claims] A cosmetic product characterized by containing a water-soluble extract of the fruit of Pigeon.