JPH0511059U - Flow path cleaning device for auto sampler - Google Patents
Flow path cleaning device for auto samplerInfo
- Publication number
- JPH0511059U JPH0511059U JP6654991U JP6654991U JPH0511059U JP H0511059 U JPH0511059 U JP H0511059U JP 6654991 U JP6654991 U JP 6654991U JP 6654991 U JP6654991 U JP 6654991U JP H0511059 U JPH0511059 U JP H0511059U
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- Prior art keywords
- cleaning
- cleaning liquid
- sample
- way valve
- port
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Links
- 238000004140 cleaning Methods 0.000 title claims abstract description 135
- 239000007788 liquid Substances 0.000 claims abstract description 134
- 238000003860 storage Methods 0.000 claims abstract description 22
- 239000000523 sample Substances 0.000 abstract description 45
- 239000012488 sample solution Substances 0.000 abstract description 20
- 238000005259 measurement Methods 0.000 abstract description 15
- 238000005406 washing Methods 0.000 abstract description 10
- 239000003960 organic solvent Substances 0.000 abstract description 8
- 239000002253 acid Substances 0.000 abstract description 3
- 239000003513 alkali Substances 0.000 abstract description 2
- 238000000034 method Methods 0.000 description 10
- 239000000243 solution Substances 0.000 description 7
- 238000004891 communication Methods 0.000 description 6
- 239000003480 eluent Substances 0.000 description 6
- 230000002378 acidificating effect Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 210000002966 serum Anatomy 0.000 description 5
- 210000002700 urine Anatomy 0.000 description 5
- 239000000203 mixture Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 239000004809 Teflon Substances 0.000 description 2
- 229920006362 Teflon® Polymers 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 239000003929 acidic solution Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 239000012670 alkaline solution Substances 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
Landscapes
- Automatic Analysis And Handling Materials Therefor (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
(57)【要約】
【目的】 試料液が流れる流路内を確実に洗浄すること
により、正確な測定が行えるオートサンプラーの流路洗
浄装置を提供すこと。
【構成】 洗浄液貯槽を複数個用意し、それら第１〜第
３の洗浄液貯槽３０ａ〜３０ｃ内には、それぞれ異なる
種類の第１〜第３の洗浄液３１ａ〜３１ｃが充填されて
いる。そしてそれら各洗浄液はそれぞれアルカリ，酸，
有機溶媒から選ばれた一種としている。第１〜第３の洗
浄液貯槽内には、それぞれ洗浄液吸入管３３の下端吸入
口３３ａが挿入配置され、その洗浄液吸入管３３の他方
端部を五方弁３４の第１〜第３のポートａ〜ｃに連結す
る。この五方弁の第４のポートｄは、計量シリンジ３５
に連繋されており、また、五方弁の第５のポートｅは、
試料液吸入管２３に接続されている。五方弁の流路切り
替えと計量シリンジの吸引・吐出を制御することによ
り、各洗浄液が試料液吸入管へ供給される。
(57) [Summary] [Purpose] To provide a flow channel cleaning device for an auto sampler that can perform accurate measurement by reliably cleaning the flow channel of a sample solution. [Structure] A plurality of cleaning liquid storage tanks are prepared, and the first to third cleaning liquid storage tanks 30a to 30c are filled with different kinds of first to third cleaning liquids 31a to 31c. And each of these cleaning liquids is alkali, acid,
It is a kind selected from organic solvents. The lower end suction port 33a of the cleaning liquid suction pipe 33 is inserted and arranged in each of the first to third cleaning liquid storage tanks, and the other end portion of the cleaning liquid suction pipe 33 is connected to the first to third ports a of the five-way valve 34. Connect to ~ c. The fourth port d of this five-way valve is
And the fifth port e of the five-way valve is
It is connected to the sample liquid suction tube 23. Each washing solution is supplied to the sample solution suction tube by controlling the flow path switching of the five-way valve and controlling the suction / discharge of the measuring syringe.
Description
【０００１】[0001]
本考案はＨＰＬＣ用のオートサンプラーに用いられ、試料液が流通する流路を 洗浄する洗浄装置の改良に関する。 The present invention relates to an improvement of a cleaning device used in an auto sampler for HPLC, which cleans a flow path through which a sample liquid flows.
【０００２】[0002]
図３に従来のオートサンプラーの測定装置の構成例を一例を示している。同図 に基づいてその動作原理を簡単に説明する。まず、６個のポートＡ〜Ｆを備えた 六方弁１は、試料導入ルートと試料導出ルートとを切り替えるためのものであり 、実線で示す流路と破線で示す流路のいずれか一方が選択的に導通される。この 実線で示す流路を導通させた状態で、試料液吸入管２を下降移動させて、その先 端に設けられたニードル３を試料瓶４内に充填された試料液５内に挿入する。次 いで、計量シリンジ６のピストンをひいて、ニードル３から試料液５を所定量吸 引する。 FIG. 3 shows an example of the configuration of a conventional measuring device for an autosampler. The operating principle will be briefly described with reference to FIG. First, the six-way valve 1 provided with six ports A to F is for switching between the sample introduction route and the sample derivation route, and one of the flow paths shown by the solid line and the broken line is selected. Be electrically conducted. The sample liquid suction pipe 2 is moved downward while the flow path indicated by the solid line is in conduction, and the needle 3 provided at the tip thereof is inserted into the sample liquid 5 filled in the sample bottle 4. Next, the piston of the measuring syringe 6 is pulled and the sample liquid 5 is sucked from the needle 3 by a predetermined amount.
【０００３】 次いで、六方弁１の流路を切り替えて破線に示す状態とする。この状態で計量 シリンジ６のピストンを押して所定量だけサンプルループ７内に試料液を注入す る。Next, the flow path of the six-way valve 1 is switched to the state shown by the broken line. In this state, the piston of the measuring syringe 6 is pushed to inject the sample solution into the sample loop 7 by a predetermined amount.
【０００４】 その後、再び六方弁１の流路を切り替えて、元の実線に示す状態にする。そし て、ポンプ８によって貯槽９内の溶離液１０がサンプルループ内の試料をカラム １１へ送出する。試料はカラム１１内で各成分に分離され検出器１２で検知され る。After that, the flow path of the six-way valve 1 is switched again to the state shown by the original solid line. Then, the eluent 10 in the storage tank 9 delivers the sample in the sample loop to the column 11 by the pump 8. The sample is separated into each component in the column 11 and detected by the detector 12.
【０００５】 そして、一回の測定が終了したなら、次回の測定に備えて各流路内の洗浄を行 う。ここでまず上記の試料液５の押し出しの際に溶離液１０が通過した流路内は その溶離液１０で洗浄される。しかし、溶離液１０の通過しない経路、すなわち 、ニードル３並びに試料液吸入管２等は溶離液１０では洗浄されない。そこで従 来は、三方弁１３の流路を切り替えて破線に示す状態とし、その状態で計量シリ ンジ６のピストンを引いて計量シリンジ６内に洗浄液貯槽１４内に充填された洗 浄液１５を吸入させた後、三方弁１３の流路を切り替えて元の実線の状態に戻す 。そして、ピストンを押すことにより計量シリンジ６内の洗浄液１５を試料液吸 入管２を介してニードル３側へ供給し、そのニードル３より外部へ吐出させる。 すなわち、上記の試料液の吸入と逆方向に洗浄液１５を流すことになる。これに より試料液注入管２並びにニードル３等の内部が洗浄される。Then, when one measurement is completed, the inside of each flow path is cleaned in preparation for the next measurement. Here, first, the inside of the channel through which the eluent 10 has passed when the sample solution 5 is extruded is washed with the eluent 10. However, the path through which the eluent 10 does not pass, that is, the needle 3 and the sample liquid suction pipe 2 are not washed with the eluent 10. Therefore, conventionally, the flow path of the three-way valve 13 is switched to the state shown by the broken line, and in this state, the piston of the measuring syringe 6 is pulled to remove the cleaning liquid 15 filled in the cleaning liquid storage tank 14 into the measuring syringe 6. After inhaling, the flow path of the three-way valve 13 is switched to restore the original solid line state. Then, by pushing the piston, the cleaning liquid 15 in the measuring syringe 6 is supplied to the needle 3 side through the sample liquid suction tube 2 and discharged from the needle 3 to the outside. That is, the cleaning liquid 15 is caused to flow in the direction opposite to the above-described suction of the sample liquid. As a result, the inside of the sample liquid injection tube 2, the needle 3 and the like are cleaned.
【０００６】[0006]
ところで、上記試料液５が生体サンプル、特に血清及び尿等の場合には、その 試料液５中の蛋白質及び脂質が試料液吸入管２等の各流路を形成するテフロン（ 登録商標）に吸着する。そして、それら吸着した蛋白質等を除去するためには、 アルカリ，酸，有機溶媒各種による洗浄が必要となる。しかし、上記した従来の 装置では、単一の洗浄液貯槽１４しか有していないため、洗浄時にニードル３側 へ供給できる洗浄液１５は一種類となる。したがって、測定する試料液５の種類 に応じて、上記３種類の洗浄液のうち最も洗浄効果のあがる一種を選択し使用し ていたため、充分な洗浄効果が発揮し得ないおそれがある。すなわち、仮にアル カリ性の洗浄液を用いた場合には、酸性や有機溶媒の洗浄液で落ちる物質が除去 できずに流路内壁面に付着したままになるおそれがある。 By the way, when the sample solution 5 is a biological sample, especially serum and urine, the proteins and lipids in the sample solution 5 are adsorbed by Teflon (registered trademark) forming each flow path of the sample solution suction tube 2 and the like. To do. Then, in order to remove the adsorbed proteins and the like, washing with various kinds of alkalis, acids and organic solvents is required. However, since the conventional apparatus described above has only the single cleaning liquid storage tank 14, the cleaning liquid 15 that can be supplied to the needle 3 side during cleaning is one type. Therefore, depending on the type of the sample liquid 5 to be measured, one of the three types of cleaning liquid having the highest cleaning effect was selected and used, and there is a possibility that a sufficient cleaning effect may not be exhibited. That is, if an alkaline cleaning liquid is used, the substances that fall off with an acidic or organic solvent cleaning liquid may not be removed and may remain attached to the inner wall surface of the channel.
【０００７】 さらに、上記選択されるべき洗浄液は試料液の成分等により異なるため、試料 毎に最適な洗浄液を用いるためには、測定の都度に洗浄液１５すなわち洗浄液貯 槽１４の交換作業を行わなければならず、その作業が繁雑で作業効率がおちる。 しかも、複数の試料液に対してその測定から洗浄まで自動的に行うオートサンプ ラの場合には、試料液ごとの洗浄液の交換は困難であり、その結果ある試料液に 対しては最適ではない洗浄液を用いて測定後の洗浄を行うことになり、洗浄効果 が低下すると言う問題も有する。Further, since the cleaning liquid to be selected differs depending on the components of the sample liquid and the like, in order to use the optimum cleaning liquid for each sample, the cleaning liquid 15, that is, the cleaning liquid storage tank 14 must be replaced every time measurement is performed. However, the work is complicated and the work efficiency is reduced. Moreover, in the case of an autosampler that automatically performs measurement to cleaning for multiple sample solutions, it is difficult to change the cleaning solution for each sample solution, and as a result, it is not optimal for a given sample solution. Since the cleaning is performed after the measurement using the cleaning liquid, there is also a problem that the cleaning effect is reduced.
【０００８】 本考案は上記した背景に鑑みてなされたもので、その目的とするところは、試 料液の種類に関係なく流路を確実に洗浄することにより、正確な測定を行うこと ができるオートサンプラーの流路洗浄装置を提供することにある。The present invention has been made in view of the above background, and an object thereof is to perform accurate measurement by surely cleaning the flow path regardless of the type of the sample liquid. An object is to provide a flow path cleaning device for an auto sampler.
【０００９】[0009]
上記した目的を達成するために、本考案に係るオートサンプラーの流路洗浄装 置では、異なる洗浄液が充填された複数の洗浄液貯槽と、前記洗浄液を吸入する と共に、その吸入した洗浄液をオートサンプラー用の試料液吸入管に供給するシ リンジと、前記複数の洗浄液貯槽と前記シリンジとを結ぶ流路の間に挿入配置さ れ、所望の洗浄液を前記シリンジに連通可能にする切り替え手段とを設けた。 In order to achieve the above-mentioned object, in the flow path cleaning device of the auto sampler according to the present invention, a plurality of cleaning liquid storage tanks filled with different cleaning liquids and the cleaning liquids are sucked, and the sucked cleaning liquids are used for the auto sampler. And a switching means that is inserted and arranged between the plurality of washing liquid storage tanks and the syringe to connect the desired washing liquid to the syringe. ..
【００１０】[0010]
切り替え手段を適宜切り替えることにより、複数の洗浄液貯槽に充填された洗 浄液のうち、所望のものを一旦シリンジ内に吸入し、それを試料液吸入管側へ供 給する。したがって、切り替え手段を順次切り替えながら洗浄処理をすることに より、複数の洗浄液を用いて流路内の洗浄を行うことができる。その結果、例え ば血清や尿等の複数種の洗浄液を用いなければ完全に洗浄を行うことができない 試料液の測定後であっても確実に洗浄される。 By appropriately switching the switching means, a desired cleaning liquid among the cleaning liquids filled in the plurality of cleaning liquid storage tanks is temporarily sucked into the syringe and then supplied to the sample liquid suction tube side. Therefore, by performing the cleaning process while sequentially switching the switching means, it is possible to clean the inside of the flow path using a plurality of cleaning liquids. As a result, it is possible to completely wash the sample liquid without using multiple types of washing liquid such as serum and urine.
【００１１】[0011]
以下本考案に係るオートサンプラーの流路洗浄装置の好適な実施例を添付図面 を参照にして詳述する。図１は本考案の第１実施例を適用したオートサンプラー の測定装置の一例を示している。同図に示すように、測定系の基本構成は上記し た従来のものと略同一構成を採っている。すなわち、六方弁２０を適宜切替える ことにより、試料瓶２１内の試料液２２をニードル２３ａから吸い込むと共に試 料液吸入管２３を介して一旦サンプルループ２４内に貯留させた後、ポンプ２５ によって貯槽２６内の溶離液２７をサンプルループ２４側に供給することにより 、上記一旦貯留されていた試料液２２をカラム２８ひいては検出器２９に供給す るようにしている。 Hereinafter, a preferred embodiment of a flow path cleaning device for an auto sampler according to the present invention will be described in detail with reference to the accompanying drawings. FIG. 1 shows an example of a measuring device of an auto sampler to which the first embodiment of the present invention is applied. As shown in the figure, the basic configuration of the measurement system is approximately the same as the conventional configuration described above. That is, by appropriately switching the six-way valve 20, the sample solution 22 in the sample bottle 21 is sucked from the needle 23a and temporarily stored in the sample loop 24 via the sample solution suction tube 23, and then the pump 25 is used to store the sample solution in the storage tank 26. By supplying the eluent 27 in the inside to the sample loop 24 side, the once stored sample solution 22 is supplied to the column 28 and further to the detector 29.
【００１２】 ここで本考案では、洗浄液貯槽を複数個（本例では３個）用意し、それら第１ 〜第３の洗浄液貯槽３０ａ〜３０ｃ内には、それぞれ異なる種類の第１〜第３の 洗浄液３１ａ〜３１ｃが充填されている。そしてそれら各洗浄液３１ａ〜３１ｃ はそれぞれアルカリ，酸，有機溶媒から選ばれた一種とするのが好ましい。Here, in the present invention, a plurality (three in this example) of cleaning liquid storage tanks are prepared, and the first to third cleaning liquid storage tanks 30a to 30c respectively have different types of first to third cleaning liquid storage tanks. The cleaning liquids 31a to 31c are filled. Each of the cleaning liquids 31a to 31c is preferably one selected from alkali, acid and organic solvents.
【００１３】 第１〜第３の洗浄液貯槽３０ａ〜３０ｃ内には、それぞれ洗浄液吸入管３３の 下端吸入口３３ａが挿入配置され、その洗浄液吸入管３３の他方端部を切り替え 手段たる五方弁３４の第１〜第３のポートａ〜ｃに連結する。一方、この五方弁 ３４の第４のポートｄは、計量シリンジ３５に連繋されており、また、五方弁３ ４の第５のポートｅは、試料液吸入管２３を介して六方弁２０のポートＦに接続 されている。A lower end suction port 33a of a cleaning liquid suction pipe 33 is inserted and arranged in each of the first to third cleaning liquid storage tanks 30a to 30c, and a five-way valve 34 as a means for switching the other end of the cleaning liquid suction pipe 33. To the first to third ports a to c. On the other hand, the fourth port d of the five-way valve 34 is connected to the measuring syringe 35, and the fifth port e of the five-way valve 34 is connected to the six-way valve 20 via the sample liquid suction pipe 23. It is connected to port F of.
【００１４】 この五方弁３４は、切り替え操作により計量シリンジ３５が接続された第４の ポートｄと、第１〜第３，第５のポートａ〜ｃ，ｅから選ばれた１のポートとが 連通状態となる。そして、その五方弁３４の切り替え操作と計量シリンジ３５の ピストン３６の押し引き操作を適宜に行うことにより、試料液２２の吸引や、試 料液吸入管２３並びに試料液吸入管２３の先端に装着されたニードル２３ａの主 として内側流路の洗浄が行われる。尚、図中符合３９は、洗浄ポートである。The five-way valve 34 has a fourth port d to which the measuring syringe 35 is connected by a switching operation, and one port selected from the first to third and fifth ports a to c and e. Are connected. By appropriately performing the switching operation of the five-way valve 34 and the pushing / pulling operation of the piston 36 of the measuring syringe 35, the sample liquid 22 is sucked, the sample liquid suction pipe 23 and the tip of the sample liquid suction pipe 23 are sucked. The inner flow passage is mainly washed by the attached needle 23a. Incidentally, reference numeral 39 in the figure is a cleaning port.
【００１５】 上記構成の装置の使用方法の一例について説明する。今、通常の試料液２２の 測定が終了したとする。すると、ニードル２３ａを所定方向に移動させて、図示 する如くそのニードル２３ａの先端を洗浄ポート３９内に挿入配置させる。そし て、五方弁３４を切り替えて第１のポートａと第４のポートｄとが連通する状態 にした後、計量シリンジ３５のピストン３６を所定量引くことにより計量シリン ジ３５内に第１の洗浄液３０ａを所定量吸入する。An example of a method of using the device having the above configuration will be described. Now, it is assumed that the normal measurement of the sample liquid 22 is completed. Then, the needle 23a is moved in a predetermined direction, and the tip of the needle 23a is inserted and arranged in the cleaning port 39 as shown in the drawing. Then, after switching the five-way valve 34 so that the first port a and the fourth port d are in communication with each other, the piston 36 of the measuring syringe 35 is pulled by a predetermined amount, so that the first syringe is placed in the measuring syringe 35. A predetermined amount of the cleaning liquid 30a is sucked.
【００１６】 次いで、五方弁３４の流路を切り替え、図中に実線で示すように第４のポート ｄと第５のポートｅとが連通する状態にする。この状態で、計量シリンジ３５の ピストン３６を押すことにより、計量シリンジ３５内に吸入された第１の洗浄液 （例えばアルカリ性）３０ａは、試料液吸入管２３，六方弁２０のポートＡ，Ｆ 間並びにニードル２３ａを通ってニードル２３ａの下端より洗浄ポート３９内に 向けて吐出される。これにより各内側流路の洗浄が行われる。この作業により流 路内に付着している物質のうち、アルカリ性の洗浄液により溶解されるものが除 去され、外部に排出されることになる。尚、上記の工程を必要に応じて複数回繰 り返し行うようにしても良い。Next, the flow path of the five-way valve 34 is switched so that the fourth port d and the fifth port e communicate with each other as shown by the solid line in the figure. In this state, by pushing the piston 36 of the measuring syringe 35, the first cleaning liquid (for example, alkaline) 30a sucked into the measuring syringe 35 is discharged between the sample liquid suction pipe 23, the ports A and F of the six-way valve 20, and the like. It is discharged from the lower end of the needle 23a through the needle 23a toward the inside of the cleaning port 39. As a result, each inner channel is washed. By this work, the substances adhering to the inside of the channel will be removed and those that will be dissolved by the alkaline cleaning solution will be discharged to the outside. The above steps may be repeated a plurality of times as necessary.
【００１７】 そして、アルカリ性の第１の洗浄液３０ａによる洗浄処理が終了したなら、次 ぎに第２の洗浄液３０ｂ（例えば酸性）による洗浄処理に移る。すなわち、五方 弁３４の流路を切り替え、第４のポートｄと第２のポートｂとが連通する状態に し、この状態で計量シリンジ３５のピストン３６を引くことにより計量シリンジ ３５内に所定量の第２の洗浄液３０ｂが吸入される。この吸入量は、上記第１の 洗浄液３０ａの吸入量と必ずしも同量にする必要はなく、洗浄対象となる測定試 料液の組成により適宜選定される。When the cleaning treatment with the alkaline first cleaning liquid 30a is completed, the cleaning treatment with the second cleaning liquid 30b (for example, acidic) is performed next. That is, the flow path of the five-way valve 34 is switched so that the fourth port d and the second port b are in communication with each other, and in this state, the piston 36 of the measuring syringe 35 is pulled to locate the inside of the measuring syringe 35. A fixed amount of the second cleaning liquid 30b is inhaled. This inhalation amount does not necessarily have to be the same as the inhalation amount of the first cleaning liquid 30a, and is appropriately selected according to the composition of the measurement sample liquid to be cleaned.
【００１８】 以後、上記第１の洗浄液３０ａの洗浄処理と同様に五方弁を切り替えると共に ピストン３６を押し込むことにより、第２の洗浄液３０ｂを試料液吸入管２３側 へ供給し、その流通に伴い流路内に付着している物質のうち、酸性の洗浄液によ り溶解されるものが除去され、その洗浄液３０ｂと共に外部に排出されることに なる。尚、この第２の洗浄液３０ｂの洗浄工程も必要に応じて複数回繰り返し行 うようにしても良い。Thereafter, as in the case of the cleaning process of the first cleaning liquid 30a, the five-way valve is switched and the piston 36 is pushed in to supply the second cleaning liquid 30b to the sample liquid suction pipe 23 side, and along with its circulation. Of the substances adhering to the inside of the flow path, those that are dissolved by the acidic cleaning liquid are removed and are discharged to the outside together with the cleaning liquid 30b. The cleaning process of the second cleaning liquid 30b may be repeated a plurality of times as necessary.
【００１９】 次いで、最終の第３の洗浄液３０ｃ（例えば、有機溶媒）による洗浄処理に移 る。すなわち、五方弁３４の流路を切り替え、第４のポートｄと第３のポートｃ とが連通する状態にし、この状態で計量シリンジ３５のピストン３６を引くこと により計量シリンジ３５内に所定量の第３の洗浄液３０ｃが吸入される。この吸 入量は、上記第１の洗浄液３０ａの吸入量と必ずしも同量にする必要はなく、洗 浄対象となる測定試料液の組成により適宜選定される。Next, the final cleaning process with the third cleaning liquid 30c (for example, an organic solvent) is performed. That is, the flow path of the five-way valve 34 is switched so that the fourth port d and the third port c are in communication with each other, and in this state, the piston 36 of the measuring syringe 35 is pulled to bring the predetermined amount into the measuring syringe 35. The third cleaning liquid 30c is sucked. This suction amount does not necessarily have to be the same as the suction amount of the first cleaning liquid 30a, and is appropriately selected according to the composition of the measurement sample liquid to be cleaned.
【００２０】 以後、上記両洗浄液３０ａ，ｂの洗浄処理と同様の操作により、第３の洗浄液 ３０ｃの流通に伴い流路内に付着している物質のうち、有機溶媒の洗浄液により 溶解されるものが除去され、その洗浄液３０ｃと共に外部に排出されることにな る。尚、この第３の洗浄液３０ｂの洗浄工程も必要に応じて複数回繰り返し行う ようにしても良い。Thereafter, by the same operation as the cleaning treatment of both the cleaning liquids 30a and 30b, among the substances adhering to the inside of the flow path as the third cleaning liquid 30c flows, those which are dissolved by the cleaning liquid of the organic solvent. Are removed and are discharged to the outside together with the cleaning liquid 30c. The cleaning process of the third cleaning liquid 30b may be repeated a plurality of times as necessary.
【００２１】 この実施例によれば、３種類の洗浄液を試料液吸入管２３内に流すことができ るため、その試料液がたとえ血清や尿等の場合であっても係る３つの洗浄液によ り確実に洗浄することができる。尚、上記した使用例では、３つの洗浄液のすべ てを使用する場合について説明したが、本考案の適用例はこれに限ることなく、 試料液の組成に応じて１または２種の洗浄液を使用するようにしても良い。すな わち、例えばオートサンプラにおいて酸性溶液に溶解しやすい試料液とアルカリ 性溶液に溶解しやすい試料液とが順次送られてきた場合には、最初の試料液の測 定を行った後の洗浄処理は酸性の第２の洗浄液３０ｂのみを使用して行い、次ぎ の試料液の測定を行った後の洗浄処理は第１の洗浄液３０ａのみを使用して行え ば良いのである。According to this embodiment, since three kinds of cleaning liquids can be flown into the sample liquid suction pipe 23, even if the sample liquid is serum or urine, the three cleaning liquids can be used. It can be surely cleaned. In the above-mentioned usage example, the case where all three cleaning solutions are used has been described, but the application example of the present invention is not limited to this, and one or two cleaning solutions are used depending on the composition of the sample solution. It may be done. That is, for example, in the autosampler, when a sample solution that is easily dissolved in an acidic solution and a sample solution that is easily dissolved in an alkaline solution are sent in sequence, after the first sample solution is measured, The cleaning treatment may be performed using only the acidic second cleaning liquid 30b, and the subsequent cleaning treatment after the measurement of the sample liquid may be performed using only the first cleaning liquid 30a.
【００２２】 さらにまた、上記した実施例では、使用する３つの洗浄液３０ａ〜３０ｃとし て、アルカリ性，酸性，有機溶媒の３種類の中からそれぞれ１個づつを選択する ようにしたが、例えば測定対象の試料液の組成に応じて同一種類で異なる洗浄液 例えば有機溶媒の中から異なる３つの洗浄液を選択し、充填するようにしても良 い。Furthermore, in the above-mentioned embodiment, the three cleaning liquids 30a to 30c to be used are each selected from the three types of alkaline, acidic, and organic solvents. It is also possible to select different cleaning solutions of the same type according to the composition of the sample solution, for example, three different cleaning solutions from organic solvents and fill them.
【００２３】 また、かかる３回の洗浄の際、その都度計量シリンジ３５内に各洗浄液を一旦 吸入するようにしたが、計量シリンジ３５の容量等の関係によるが、一回の吸引 時に、時分割的に五方弁３４の流路を適宜切り替えることにより、計量シリンジ ３５内に複数種の洗浄液を同時に吸入し、それを一度に放出することにより洗浄 を行うようにしても良い。かかる構成にすることにより、洗浄液の計量シリンジ ３５から試料液吸入管２３内への供給工程並びに五方弁３４の切り替え操作数を 減少できる。Further, although each cleaning liquid is sucked into the measuring syringe 35 once for each of the three washings, it depends on the capacity of the measuring syringe 35, etc. By appropriately switching the flow path of the five-way valve 34, a plurality of types of cleaning liquid may be simultaneously sucked into the measuring syringe 35 and discharged at one time for cleaning. With such a configuration, it is possible to reduce the number of steps of supplying the washing liquid from the measuring syringe 35 into the sample liquid suction pipe 23 and the number of switching operations of the five-way valve 34.
【００２４】 図２は本考案の第２実施例に係る装置の一例を示している。同図に示す様に、 本例では電気的な電磁弁を用いて洗浄液の切り替えを行っている。すなわち、そ れぞれ性質の異なる第１〜第３の洗浄液４０ａ〜４０ｃの充填された第１〜第３ の洗浄液貯槽４１ａ〜４１ｃを配設する。そして、それら第１〜第３の洗浄液貯 槽４１ａ〜４１ｃ内には、それぞれ洗浄液吸入管４３の下端吸入口４３ａが挿入 配置され、その洗浄液吸入管４３の他方端部を切り替え手段たる電磁弁装置４４ の各弁部４４ａ〜４４ｃの入力側に接続されている。この電磁弁装置４４は、各 弁部４４ａ〜４４ｃを独立して開閉させることができるようになっており、任意 の一つの弁部のみを開いたり、或いは複数の弁を同時に開いたりすることができ るようになっている。FIG. 2 shows an example of an apparatus according to the second embodiment of the present invention. As shown in the figure, in this example, the cleaning liquid is switched using an electric solenoid valve. That is, the first to third cleaning liquid storage tanks 41a to 41c filled with the first to third cleaning liquids 40a to 40c having different properties are arranged. The lower end suction port 43a of the cleaning liquid suction pipe 43 is inserted and arranged in each of the first to third cleaning liquid storage tanks 41a to 41c, and the other end of the cleaning liquid suction pipe 43 is a solenoid valve device serving as switching means. 44 is connected to the input side of each valve portion 44a to 44c. The solenoid valve device 44 can open and close each of the valve portions 44a to 44c independently, and can open only one arbitrary valve portion or a plurality of valves simultaneously. You can do it.
【００２５】 さらに、これら各弁部４４ａ〜４４ｃの出力側は、一方の洗浄液供給管４５に 連通され、この洗浄液供給管４５の端部が、三方弁４６の第１のポートａに接続 されている。また、この三方弁４６の第２のポートｂには、計量シリンジ４７が 連繋されるとともに、第３のポートｃに試料液吸入管４８が接続されている。Further, the output side of each of the valve portions 44 a to 44 c is connected to one cleaning liquid supply pipe 45, and the end portion of the cleaning liquid supply pipe 45 is connected to the first port a of the three-way valve 46. There is. A metering syringe 47 is connected to the second port b of the three-way valve 46, and a sample liquid suction pipe 48 is connected to the third port c.
【００２６】 次ぎに、本実施例の使用方法の一例について説明する。上記した第１実施例と 同様の処理を行うには、まず三方弁４６の流路を図中破線で示すように第１のポ ートａと第２のポートｂとを連通状態とする。そして、電磁弁装置４４の第１の 弁部４４ａのみを開状態とする。これにより、計量シリンジ４７と第１の洗浄液 ４０ａとが連通状態となる。したがって、ピストン４９を引くことにより計量シ リンジ４７内に第１の洗浄液４０ａを所定量注入することができ、その後三方弁 ４６の流路を切り替えて第２のポートｂと第３のポートｃとを連通した状態でピ ストン４９を押すことにより計量シリンジ４７内の第１の洗浄液４０ａを試料液 吸入管４８側に供給することができる。以後、順次電磁弁装置４４を第２の弁部 ４４ｂのみ、第３の弁部４４ｃのみをそれぞれ開状態にすると共に、三方弁４６ の切り替え並びに計量シリンジ４７のピストン４９の押し引き操作を行うことに より、第２の洗浄液４０ｂによる洗浄処理、並びに第３の洗浄液４０ｃによる洗 浄処理を順次行うことができる。Next, an example of a method of using this embodiment will be described. In order to perform the same processing as in the above-described first embodiment, first, the flow path of the three-way valve 46 is brought into communication with the first port a and the second port b as shown by the broken line in the figure. Then, only the first valve portion 44a of the solenoid valve device 44 is opened. As a result, the measuring syringe 47 and the first cleaning liquid 40a are in communication with each other. Therefore, by pulling the piston 49, a predetermined amount of the first cleaning liquid 40a can be injected into the metering cylinder 47, and then the flow path of the three-way valve 46 is switched to switch between the second port b and the third port c. By pressing the piston 49 in a state in which the first washing liquid 40a is in communication with the first washing liquid 40a, the first washing liquid 40a in the measuring syringe 47 can be supplied to the sample liquid suction pipe 48 side. Thereafter, the solenoid valve device 44 is sequentially opened only for the second valve portion 44b and only for the third valve portion 44c, and the three-way valve 46 is switched and the piston 49 of the measuring syringe 47 is pushed and pulled. Thus, the cleaning process with the second cleaning liquid 40b and the cleaning process with the third cleaning liquid 40c can be sequentially performed.
【００２７】 また、かかる複数の弁部４４ａ〜４４ｃの開閉の制御を時分割的に行い各弁部 ４４ａ〜４４ｃの開状態の時間を異ならせることにより、各洗浄液を所望の比率 で計量シリンジ４７内に一旦吸入することができる。すなわち、洗浄する試料液 に対する最適の混合比の洗浄液を容易に作成・供給することができる。尚、その 他の構成並びに作用効果は上記した第１実施例と同様であるため、その詳細な説 明を省略する。Further, by controlling the opening and closing of the plurality of valve portions 44a to 44c in a time-division manner and making the open time of each valve portion 44a to 44c different, each cleaning liquid is measured at a desired ratio. It can be inhaled once. That is, it is possible to easily prepare and supply the cleaning liquid having the optimum mixing ratio with respect to the sample liquid to be cleaned. Since the other constructions and effects are the same as those of the first embodiment, detailed description thereof will be omitted.
【００２８】 尚、上記した各実施例では、３つの洗浄液を用いる例について説明したが、本 考案はこれに限ることなく、２個或いは４個以上の任意の数にすることができる のはもちろんである。In each of the above-described embodiments, an example in which three cleaning liquids are used has been described, but the present invention is not limited to this, and it is needless to say that the number can be two or four or more. Is.
【００２９】[0029]
以上のように、本考案に係るオートサンプラーの流路洗浄装置では、複数の洗 浄液を用いて流路内の洗浄を行うことができるため、より確実に洗浄することが でき、例えば血清や尿等の複数種の洗浄液を用いなければ完全に洗浄を行うこと ができない試料液の測定後であっても確実に洗浄処理を行うことができる。その 結果、係る血清及び尿等の中の蛋白質及び脂質が試料液吸入管等の各流路を形成 するテフロン（登録商標）に吸着することがないため、流路内の容積（断面積） は常に一定に保たれるとともに、測定対象の試料液と前回行った試料液とが混在 したりすることもなく、正確な測定を行うことができる。さらに、異なる洗浄液 の必要な試料液に対しても連続して測定を行うことができ、作業効率が向上する 。 As described above, in the flow channel cleaning device for the auto sampler according to the present invention, since the flow channel can be cleaned using a plurality of cleaning liquids, more reliable cleaning can be performed, such as serum and It is possible to perform the cleaning process reliably even after the measurement of the sample solution, which cannot be completely cleaned without using plural kinds of cleaning solutions such as urine. As a result, the proteins and lipids in the serum and urine are not adsorbed on the Teflon (registered trademark) forming each flow path such as the sample liquid suction tube, so that the volume (cross-sectional area) in the flow path is It is always kept constant, and accurate measurement can be performed without the sample liquid to be measured and the sample liquid performed last time being mixed. Furthermore, it is possible to perform continuous measurements on sample liquids that require different cleaning liquids, thus improving work efficiency.
【図１】本考案に係るオートサンプラーの流路洗浄装置
の第１実施例を示す構成図である。FIG. 1 is a configuration diagram showing a first embodiment of a flow path cleaning device for an auto sampler according to the present invention.
【図２】本考案に係るオートサンプラーの流路洗浄装置
の第２実施例を示す要部構成図である。FIG. 2 is a main part configuration diagram showing a second embodiment of the flow path cleaning apparatus of the autosampler according to the present invention.
【図３】従来例を示す構成図である。FIG. 3 is a configuration diagram showing a conventional example.
３０ａ，４１ａ 第１の洗浄液貯槽 ３０ｂ，４１ｂ 第２の洗浄液貯槽 ３０ｃ，４１ｃ 第３の洗浄液貯槽 ３１ａ，４０ａ 第１の洗浄液 ３１ｂ，４０ｂ 第２の洗浄液 ３１ｃ，４０ｃ 第３の洗浄液 ３５，４１ 計量シリンジ ３４ 五方弁（切り替え手段） ４４ 電磁弁装置（切り替え弁） 30a, 41a First cleaning liquid storage tank 30b, 41b Second cleaning liquid storage tank 30c, 41c Third cleaning liquid storage tank 31a, 40a First cleaning liquid 31b, 40b Second cleaning liquid 31c, 40c Third cleaning liquid 35, 41 Measuring syringe 34 Five-way valve (switching means) 44 Electromagnetic valve device (switching valve)
Claims (1)
貯槽と、 前記洗浄液を吸入すると共に、その吸入した洗浄液をオ
ートサンプラー用の試料液吸入管に供給するシリンジ
と、 前記複数の洗浄液貯槽と前記シリンジとを結ぶ流路の間
に挿入配置され、所望の洗浄液を前記シリンジに連通可
能にする切り替え手段とを備えたことを特徴とするオー
トサンプラーの流路洗浄装置。Claims for utility model registration: 1. A plurality of cleaning liquid storage tanks filled with different cleaning liquids, and a syringe for sucking the cleaning liquids and supplying the sucked cleaning liquids to a sample liquid suction pipe for an autosampler. A flow channel cleaning device for an auto sampler, which is disposed between the flow channels connecting the plurality of cleaning liquid storage tanks and the syringe, and is provided with a switching unit that allows a desired cleaning liquid to communicate with the syringe. ..
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6654991U JPH0511059U (en) | 1991-07-29 | 1991-07-29 | Flow path cleaning device for auto sampler |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP6654991U JPH0511059U (en) | 1991-07-29 | 1991-07-29 | Flow path cleaning device for auto sampler |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH0511059U true JPH0511059U (en) | 1993-02-12 |
Family
ID=13319104
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP6654991U Withdrawn JPH0511059U (en) | 1991-07-29 | 1991-07-29 | Flow path cleaning device for auto sampler |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0511059U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112858659A (en) * | 2019-11-28 | 2021-05-28 | 深圳市帝迈生物技术有限公司 | Sampling needle cleaning system, sample analyzer and immune luminescence analyzer |
| US20220184617A1 (en) * | 2019-04-12 | 2022-06-16 | Shimadzu Corporation | Microfluidic system |
| CN116893131A (en) * | 2023-09-11 | 2023-10-17 | 深圳市帝迈生物技术有限公司 | Flow type sample analyzer and flow chamber cleaning method |
-
1991
- 1991-07-29 JP JP6654991U patent/JPH0511059U/en not_active Withdrawn
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20220184617A1 (en) * | 2019-04-12 | 2022-06-16 | Shimadzu Corporation | Microfluidic system |
| US12285756B2 (en) * | 2019-04-12 | 2025-04-29 | Shimadzu Corporation | Microfluidic system |
| CN112858659A (en) * | 2019-11-28 | 2021-05-28 | 深圳市帝迈生物技术有限公司 | Sampling needle cleaning system, sample analyzer and immune luminescence analyzer |
| CN116893131A (en) * | 2023-09-11 | 2023-10-17 | 深圳市帝迈生物技术有限公司 | Flow type sample analyzer and flow chamber cleaning method |
| CN116893131B (en) * | 2023-09-11 | 2024-02-20 | 深圳市帝迈生物技术有限公司 | Flow type sample analyzer and flow chamber cleaning method |
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