JPH05163132A - Skin cosmetic - Google Patents

Abstract

PURPOSE:To obtain a skin cosmetic comprising a collagenase production promoting substance and a fibroblast activating soluble shell membrane, improving skin action, having excellently inhibitory action on aging of skin. CONSTITUTION:A collagenase production promoting substance such as partial hydrolyzate of silk, serines or ethanolamine is combined with a soluble shell membrane, a substance to activate fibroblast to give a skin cosmetic. The combined two substances of this skin cosmetic work without obstructing mutual actions and raise metabolism rotation of collagen. Consequently, the skin cosmetic has improved skin function and excellently inhibitory action on aging of skin such as softening effects on skin, bettering effects on tension on of skin and ameliorating effects on wrinkles.

Description

Detailed Description of the Invention

[0001]

FIELD OF THE INVENTION The present invention enhances skin function,
The present invention relates to a skin cosmetic having an excellent anti-aging effect on the skin (effect of softening the skin, effect of improving skin elasticity, effect of improving skin wrinkles, etc.)

[0002]

BACKGROUND OF THE INVENTION Aged skin has a dry and rough skin-like state, which is thought to be due to a decrease in the water retention function of the stratum corneum, a decrease in the barrier function, and a decrease in the amount of sebum secretion. Has been. In addition, a decrease in the number of cells in both the epidermis and dermis causes a decrease in metabolic function, and further, the redox-related enzyme activity of the epidermis and the oxygen partial pressure of the skin decrease, and the turnover rate of the stratum corneum decreases. It has been known.

On the other hand, collagen and elastin are components that form most of the structure of the skin, and it is said that these influence the elasticity and flexibility of the skin. And the soluble fraction of these components decreases with aging,
It is believed that a crosslinked structure is formed, which reduces elasticity and flexibility. Furthermore, with age, the metabolism of collagen is reduced, and hyaluronic acid, which is an intercellular substance of the skin, is significantly reduced, which leads to a decrease in the water content of the skin. As a result, the aged skin becomes atrophied and thinned, loses its flexibility, elasticity and smoothness and becomes rough skin.

As an agent for improving such aged skin,
Many cosmetics containing collagen or hyaluronic acid have been proposed, but these merely improve the moisturizing effect on the skin surface and do not essentially improve aged skin. In addition, vitamins and crude drugs are used as skin cell activating agents, but the current situation is that they have not yet reached the treatment of aged skin.

Under these circumstances, in order to prevent the decrease in collagen turnover (generally, the turnover of collagen is determined by the degradation rate and synthesis rate of collagen) with aging, collagenase, which is the rate-limiting enzyme for collagen degradation, is held. As a substance that already promotes the production of collagenase by the present inventors, for example, an ethanolamine derivative (Japanese Patent Application No. 2-97071).
No. 2, Japanese Patent Application No. 2-127390, and Japanese Patent Application No. 2-212.
931), serine derivatives (Japanese Patent Application No. 2-99579 and Japanese Patent Application No. 2-186763), and partially hydrolyzed silk (Japanese Patent Application No. 3-59752).

On the other hand, cosmetics containing a soluble egg shell membrane for the purpose of activating fibroblasts have been proposed (for example, JP-A-48-40943 and JP-A-3-190808). Etc.), it is hard to say that the effect is still sufficient.

[0007] Further, for the purpose of further increasing collagen turnover, a substance that promotes collagenase synthesis at the same time as a substance that promotes collagenase production, T
When GF-β or estrogen is combined, collagenase secretion may be suppressed depending on the blending ratio.

[0008] That is, without inhibiting the action of the collagenase production-promoting substance that is added for the purpose of promoting the metabolism of collagen, it further acts on fibroblasts in some form to activate the activity. There is no cosmetics based on the idea.

[0009]

SUMMARY OF THE INVENTION Therefore, the object of the present invention is that a substance that promotes the production of collagenase and a substance that activates fibroblasts work without interfering with each other's action, thereby promoting collagen turnover. By increasing it, the skin function is enhanced, and the effect of preventing skin aging (skin softening effect,
(EN) It is intended to provide a skin cosmetic excellent in the effect of improving skin elasticity and the effect of improving skin wrinkles.

[0010]

[Means for Solving the Problems] That is, the present invention is a skin cosmetic comprising a collagenase production promoting substance and a soluble eggshell membrane.

As the collagenase production-promoting substance used in the present invention, a procollagenase-producing substance (collagenase is secreted from cells as a precursor procollagenase, and then activated in vivo by a proteolytic enzyme. Generally considered to be)), for example, silk partial hydrolyzate, serine and its derivatives, ethanolamine and its derivatives, and the like.

The silk partial hydrolyzate, especially the water-soluble silk peptide, is a known substance used in skin cosmetics and the like. For example, as its production method, JP-B-58-17763, JP-B-59-31520, and JP-B-59-31520 are disclosed. The method described in JP-B-60-41043 is known. Among the partially hydrolyzed silk, sulfuric acid hydrolyzate of silk fiber having a molecular weight of 500 or less can be mentioned as a particularly preferable one.

Examples of serine and its derivatives include L
-Serine, DL-serine, N-methyl-L-serine, N
-Methyl-DL-serine, N, N-dimethyl-L-serine, N, N-dimethyl-DL-serine and the like can be mentioned.

Examples of ethanolamine and its derivatives include monoethanolamine, N-methylethanolamine, N, N-dimethylethanolamine, 2-amino-1-butanol, 2-amino-1-propanol and N-methyl-amine. 2-amino-1-butanol, N-methyl-2-amino-1-propanol and the like can be mentioned. Ethanolamine and its derivatives are used in the form of free amines or amine salts. Examples of the amine salt include salts of mineral acids such as hydrochlorides, sulfates, nitrates and phosphates, acetates, lactates, citrates, malates, tartrates, fumarates, maleates and lower fatty acids. Examples thereof include salts of organic acids such as salts and higher fatty acid salts.

The content of the collagenase production-promoting substance used in the present invention varies depending on the dosage form, but when a sulfuric acid hydrolyzate of silk fiber is used, the content of the silk fiber is 0.5-4.
%, The content of this substance is preferably 0.1 to 10% based on the total weight of the skin cosmetic of the present invention. When serine and its derivative or ethanolamine and its derivative are used, the content thereof is preferably 0.001 to 10% with respect to the total weight of the skin cosmetic of the present invention. If the content is less than the lower limit of the above range, the effect is not sufficient, and if it exceeds the upper limit, the effect commensurate with the increased amount cannot be expected.

As the soluble eggshell membrane used in the present invention, a membrane (eggshell membrane) attached to the inside of the eggshell of an avian egg such as a chicken egg or a quail egg (egg shell membrane) is treated with an acid agent, an alkaline agent, an organic solvent, oxidation
Those generally known as those solubilized with a reducing agent and the like, for example, those obtained by the method described in JP-A-48-40943, JP-A-1-275512 and the like can be mentioned.

The content of the soluble eggshell membrane used in the present invention is preferably 0.01 to 10% by weight (hereinafter, simply expressed as%) based on the total weight of the skin cosmetic of the present invention in terms of dry matter. is there. If the content is less than 0.01%, the effect is not sufficient, and if it exceeds 10%, the effect commensurate with the increased amount cannot be expected.

The skin cosmetic of the present invention comprises lotions, emulsions, creams, ointments, packs,
It is possible to make a dosage form such as powders. Further, the skin cosmetic of the present invention includes a surfactant, a moisturizer, a pH adjuster, a thickener, a bactericide, an antiseptic, a keratolytic agent, an antioxidant,
Fragrances, dyes, ultraviolet absorbers, pigments and the like can be appropriately added within a range that achieves the object of the present invention.

[0019]

The skin cosmetic of the present invention contains a collagenase production-promoting substance and a soluble eggshell membrane having an action of activating fibroblasts, both of which work without disturbing each other's action and collagen turnover. Based on the action of increasing the skin's function, it enhances the skin function and gives an antiaging effect on the skin (skin softening effect, skin ameliorating effect, skin wrinkle improving effect, etc.).

[0020]

The present invention will be described in more detail with reference to the following examples. The blending amount (%) described below means% by weight. The skin viscoelasticity test method and skin beautifying effect test method described in Examples are as follows.

(1) Skin viscoelasticity test method Wistar hairless rats (6 weeks old, male, 1 group 5)
The back of each animal was shaved, and 0.1 g of the sample was applied daily to the 2 × 2 cm site on the right shoulder. 45 days after the start of the test, the skin viscoelasticity value was measured using the surface viscoelasticity measuring device described in Japanese Patent Application No. 2-278505, and the average value of the skin viscoelasticity value of each group was obtained. In addition, the skin viscoelasticity value (arbitrary unit) displayed by the same measuring device shows a value as the skin becomes harder.

(2) Skin beautification test method Female subjects (35-5) who complain of rough skin, fine wrinkles, dry skin, etc.
20 people (5 years old) were allowed to take the sample twice a day (morning and evening) for 2 consecutive months, and then evaluated for improvement of skin flexibility, suppleness, and wrinkles. The results are shown by the number of respondents who responded that "the skin flexibility was improved,""the skin acuity was improved," and "skin wrinkles were improved."

Example 1, Comparative Examples 1 to 3 [Skin Cream] Skin creams of the present invention and for comparison having the compositions shown in Table 1 were prepared, the above tests were carried out, and the results are shown in Table 2.

(1) Composition

[Table 1]

(2) Preparation method The component (C) is uniformly mixed and dissolved at about 80 ° C., and the mixture is uniformly mixed and dissolved at about 80 ° C. to the component (A) which has been uniformly mixed and dissolved. Component (B) that was uniformly mixed and dissolved in
Was added and cooled to about 30 ° C. to prepare.

(3) Characteristics

[Table 2] * 10 g of silk bleached noil is soaked in 50 ml of 40% by volume sulfuric acid and heated at 60 ° C for 12 hours, then 200 ml of cold water is added and left overnight at room temperature, and then 10N sodium hydroxide solution is gradually added to neutralize And filtered to obtain 330 ml of supernatant (containing 3% of fibroin). ** To 100 g of dried egg shell membrane dried in the sun, 1200 ml of 2N sodium hydroxide solution and 800 ml of absolute ethanol were added, and the solution obtained by treating at 40 ° C for 5 hours with stirring was filtered with a cloth filter to neutralize. , Desalted and lyophilized. *** It is shown as a relative value with the skin viscoelasticity value of Comparative Example 3 as a reference (1.0).

As can be seen from this table, the skin cream of the present invention of Example 1 showed good results over all the tests as compared with the skin creams of Comparative Examples 1-3.

Example 2 [Essence] The essence of the present invention for preparation at the time of use consisting of lotion and powder having the composition shown in Tables 3 and 4 was prepared.

(1) Composition and preparation method

[Table 3]

[0030]

[Table 4]

(2) Characteristics The skin beautifying effect test described above was divided into two groups (20 people in each group), and one group (Group A) was prepared by mixing 1 and 2 agents in appropriate amounts when using the essence of Example 2. The other group (group B) was allowed to use only one lotion. As a result, in each item, a better evaluation was obtained in the group A than in the group B.

【The invention's effect】

As described above, the skin cosmetic of the present invention is
It is obvious that it has an excellent skin aging preventing effect (skin softening effect, skin ameliorating effect, skin wrinkle improving effect, etc.).

Claims (3)

[Claims] 1. A skin cosmetic comprising a collagenase production promoting substance and a soluble eggshell membrane. 2. The collagenase production-promoting substance has a molecular weight of 5.
The skin cosmetic according to claim 1, which is a sulfuric acid hydrolyzate of silk fiber of 00 or less. 3. The skin cosmetic according to claim 1, wherein the collagenase production promoting substance is one or more selected from the group consisting of serine and its derivatives or ethanolamine and its derivatives.