JPH05201821A - Insecticidal, antimicrobial substance - Google Patents

Abstract

PURPOSE:To obtain an insecticidal, antimicrobial substance having excellent insecticidal action, antimicrobial action, prolonged effects and high safety to human body. CONSTITUTION:A purified oil of Aipinia speciosak. Schum is dispersed into a solvent with a dispersant and covered with a coating agent so that the purified oil can be durably released to give an insecticidal, antimicrobial substance. An inclusion agent such as cyclodextrin may be selected as the coating agent. A film-forming agent to make microcapsule may be selected.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an insect-repellent antibacterial substance composed of essential oil of moon peach.

[Prior Art]

The origin is India, and the moon peach (scientific name: AlPINIA SPE) often found in the Nansei Islands in Japan.
CIOSAK. SCHUM) has long been known to have a fragrance and insect repellent effect. In Okinawa, mochi is wrapped in the leaves of the moon peach, or the leaves of the moon peach are used to repel insects on the body and clothes. Therefore, research and development of products that use moon peach have been made for a long time, focusing on such characteristics of moon peach, but the moon peach essential oil extracted from moon peach is an oil. Since it is a solid substance, it does not dissolve well in a solvent such as water or ether, and its volatility is high, so that it is difficult to expect the sustainability of its effects, which is a major problem in commercialization.

[0003]

Therefore, the present invention has excellent insect repellent properties, antibacterial properties, and slow-acting properties by dispersing ginseng essential oil in a solvent with a dispersant and then coating with a coating agent. The object of the present invention is to provide an insect-repellent antibacterial substance which is highly safe for the human body.

[0004]

In order to achieve the above object, the insect-repellent antibacterial substance according to the present invention is prepared by dispersing ginseng essential oil in a solvent with a dispersant and then applying a coating treatment to enable sustained release. It will be done. Then, as the coating agent, an inclusion agent such as cyclodextrin may be selected, or a film forming agent that forms microcapsules may be selected. The main components contained in the above peach essential oil are as follows. Ingredient name% by weight ALPHA-PINENE 2.2974 CAMPHENE 17.1589 d-CANPHOR 22.9719 TERPINEOL 2.3282 p-CYMENE 1.1538 BORNEOL 1.3549 ALDEHYDE 2.1848 CRYPTONE (t) 2.333INd-α. 1.2206 N-HEXYL-ACETATE 3.4125 DIHYDROAPTOTAXENE 1.7836 PHENOL 9.0391 ALPHA-HUMUULENE 11.5217 L-CARVEOL 1.7221 LIMONE 2.3947 LINALOLOL (MXR) 1.4857. 3.7436 METTL-CINNAME 7.93 9 1, 8-CINEOL 5.0513 TERPINEN-4-01 1.7548 CARREPHYLLENE 1.2249 BETA-PHELLANDRENE 10.8871 AMYL ALCOHOL 1.2201 GAMMA-TERPNEOL 1.4733 CINEOL-ACID 1.1253L CARVY .1322 Among the above components, d-camphor, p-cymene, pinene,
Terpinene and the like have insect repellent and insecticidal activity, and also phenols, αβγ-terpinene, tinene, C-1 to C-
8 Alcohol compounds and the like are remarkably recognized as having antibacterial activity against fungi and bacteria. Here, the reason why the moon peach essential oil is dispersed is that most of the components of the moon peach essential oil are terpene oil compounds, and if such an oily substance is used as it is, it is difficult to adjust the concentration and This is because there is a major drawback in that the activity of the components is weakened due to variations in each component. Therefore, using the above-mentioned surfactant,
By dispersing the essential oil of the peach oil in the solvent, each component becomes uniform, the physiological activity of the essential oil dispersion of the peach oil becomes extremely strong, and the action peculiar to natural organic compounds (alkaloids) is strongly exerted. This is because it can be obtained.
In addition, the reason for coating the peach essential oil dispersion with a coating agent is that the peach essential oil is highly volatile, and even if it is kneaded into resin or paper as it is, the effect required for commercialization is not sustained. In order to solve the problem that cannot be expected, and as the above-mentioned coating agent, an inclusion agent such as cyclodextrin may be used, or a film-forming agent that forms microcapsules may be used. good.
In this case, as the clathrate, a crown compound, a cyclic oligosaccharide, cellulose, xanthotin, etc.,
The film-forming agent may be selected from cellulose acetate, CMC (carboxymethyl cellulose), nylon, solute carbon, gelatin, rosin, gum arabic, shellac, sodium alginate, polyester, polyamide, wax and the like. Or a mixture of two or more may be selected. The microencapsulation method using the film-forming agent may be carried out by a phase separation method, an interfacial precipitation method, or other methods.As the mechanism by which the moon peach essential oil passes through the membrane, a pore mechanism, a dissolution A diffusion mechanism or other mechanism can be selected. And the insect repellent antibacterial substance,
It is applicable not only to resins, resin molded products, paints, waxes, turfgrass regulators, agricultural chemicals, pharmaceuticals, etc., but also to all fields expected to have insect control and antibacterial properties. A method can be selected, for example, it can be mixed into the entire application or can be coated on the surface of the application. In the latter case, the insect-repellent antibacterial substance is applied to the application using a resin film. Lamine on the surface
It may be processed.

[0005]

EXAMPLES Hereinafter, a method for producing an insect-repellent antibacterial substance according to the present invention will be described. First, the peach essential oil is dispersed in water with a surfactant. For example, in the case of producing a dispersion of ginseng essential oil, purified water is added to ginseng essential oil, and a surfactant such as polyoxyethylene hydrogenated castor oil is added little by little to this with a magnetic stirrer. When uniformly mixed, the color gradually changes to a milky white color and becomes an emulsion state close to emulsification, but when the above surfactant is added and mixed and stirred, the color tone gradually changes to a transparent state. Again, purified water is added little by little, and when the color tone changes again to milky white, the surfactant is further added to adjust the final amount. It should be noted that the greater the amount of moon peach essential oil, the greater the amount of surfactant used. Therefore, if necessary, a magnetic stirrer, a high-speed mixer, or the like may be used for mixing and stirring. Further, the reason why the surfactant is used as the dispersant is that the surfactant has excellent dispersibility in natural organic compounds such as moonflower essential oil and has safety and other preferable conditions. In this example, polyoxyethylene hydrogenated castor oil is selected and used as the above-mentioned surfactant. This is because this polyoxyethylene hydrogenated castor oil is a non-ionic surfactant with low toxicity. There is also a polyoxyethylene group as it is structurally a triglyceride structure, and since it has a large molecular weight,
This is because the viscosity is high and the dispersibility is excellent. Next, in the case of obtaining an insect-repellent antibacterial substance that has been subjected to inclusion treatment with cyclodextrin in the dispersion, the appropriate amount of cyclodextrin and purified water is added to the dispersion of ginseng essential oil, and the substance characteristics are shown. Therefore, the mixing is performed smoothly and the essential oil of moon peach is uniformly included.
Further, in order to obtain an insect-repellent antibacterial substance obtained by microencapsulating the above-mentioned moon peach essential oil with a film-forming agent, for example, an aqueous gelatin solution is heated to about 40 ° C., and the aqueous gelatin solution is mixed with ethano, which is a coacervate inducer. Is added and stirred to form a so-called coacervate droplet in which gelatin as a film-forming agent is separated, and then, to this aqueous solution, ethanol is added to the above essential oil of peach, and fine particles are added. The addition of a slurry dispersed in this will result in the ginseng essential oil being coated with gelatin coacervate. Experimental Example 1 Insect repellent activity test (1) Test method for adult cockroaches A container 1 of polypropylene resin having a length and width of 25 cm × 18 cm and a height of 8 cm (see FIG. 1. A lid 2 is provided and a side surface is 4 cm in order to improve ventilation. (Put out 4 x 10 cm, and put the Saran net 3 on it), and next to (10) pellets 4 kneaded with an insect-repellent antibacterial substance in which clasped essential oil is dispersed and included.
A box 5 containing the individual pieces (see FIG. 2) and a box 6 not containing the pieces were placed. The box hole 7 (roaches entrance) was placed in the opposite direction (see FIG. 3). Further, a Petri dish 8 containing absorbent cotton soaked with water was placed on the bottom surface of the container. Then, after applying butter to the inner side surface of the container to prevent the cockroaches from escaping, the adult cockroach 9 (provided by the Japan Environmental Health Center of Watada) was released into the container, and the lid 2 (polyethylene resin 14 cm x Cut off 12 cm and put a Saran net on it, and put the container at room temperature (15-20 ° C)
After 24 hours in the dark, the number of adult cockroaches in each box was examined. (2) Test result

[Table 1] Experimental Example 2 Antibacterial activity test (1) Alternaria-sp and Aspergil
Testing method for lus-niger Alternaria-sp and Aspergillus
-Niger is diluted 10-fold with purified water, then applied over the entire surface of the medium of a square petri dish, the dispersion liquid of essential oil of ginseng is included in the filter paper, and after air-drying, it is brought into close contact with the medium as a 1 cm x 2 cm sample, The cells were continuously cultured in an incubator at 25 ° C for 5 days, and the antibacterial activity was evaluated based on the size of the inhibition zone. (JIS Z29
11 or Hello test. (2) Test Results Regarding the criteria for the efficacy test, the efficacy is considered to be so great that fungi do not develop around the specimen adhered to the medium.

[Table 2] The above antibacterial properties were exhibited. Experimental Example 3 Insecticidal activity test (1) Test method for adult mosquito mites, 0.33 cc (100 g / m ² ) of a 5% and 3% diluted solution (alcohol diluted) of moon peach essential oil on a filter paper 10 having a diameter of 5.8 cm.
12.5cc / m at 5% concentration when converted to undiluted solution
² , 6.25 cc / m ² at 3% concentration and 0.98 cc (3
When converted to a stock solution at a rate of 00 g / m ² , it is 3 at 5% concentration.
7.5cc / m ^2, 3% concentration was sprayed 18.75cc / m ^2), a glass petri dish 1 having a diameter air dried 5.8cm
1 was placed on the bottom surface of the filter paper 10, and about 50 live Agaricus communis 12 were put on the filter paper 10 and brought into contact with each other. After covering the petri dish with the Japanese paper 13, the humidity was 75% R. H. To 2
It was left for 4 hours and examined for mortality (see FIG. 4). Also,
As a control, the same examination as above was performed using a filter paper to which the essential oil of moon peach was not sprayed. (2) Test Results As shown in Table 3, the mortality of mites after leaving the peach essential oil for 24 hours and standing was 100% in both the 5% diluted solution and the 3% diluted solution.

[Table 3] Experimental Example 4 Insecticidal activity test (1) Test method for adult spider mites Wet sponge 15 placed in a 9 cm petri dish 14
On top of this, filter paper 16 is overlaid, and green beans 17
Was placed with the back side facing up, and then the back side of this leaf was inoculated with 20 budworms. Also, a 5.5 cm filter paper 19 was attached to the lid 18 side of the petri dish, and 1 ml of a liquid obtained by diluting the essential oil of moon peach coated with gelatin coacervate to a predetermined concentration with a spreading agent was soaked into the petri dish, and the lid was immediately closed. .. Then, the lid was not in a sealed state, but in a state in which a certain amount of air could flow in. (See Fig. 5) (2) Test results

[Table 4] Experimental Example 5 Insecticidal activity test (1) Test method for diamondback moth larvae In a pudding cup having a diameter of 7 cm, 5 cm x 5 cm pieces of cabbage leaf were placed, 6 larvae of diamondback moth were inoculated per cup, and then 1 cm x 1 cm filter paper was placed. One piece was placed on a cabbage leaf, and 60 μl of a solution prepared by diluting the essential oil of moon peach coated with gelatin coacervate to a predetermined concentration with a spreading agent was soaked into the cabbage leaf. Immediately, the lid was put on and the mortality rate after 24 hours and 48 hours was measured. Examined. (See FIG. 6) In FIG. 6, 20 is a pudding cup, 21 is a filter paper, and 22 is a cabbage leaf. (2) Test result

[Table 5] Experimental Example 6 Insecticidal activity test (1) Test method for green leafhopper larvae and brown planthopper larvae 5.5 cm of a pudding cup was impregnated with 1 ml of a dispersion liquid of the essential oil of moon peach essential oil, and the insects entered the cup. Cover with tissue paper to prevent
It was placed in a small insect breeding container of × 17 × 10 cm. Next, a rice seedling pot densely planted as a bait was also placed in the rearing container, and 20 larvae were inoculated per cage for 24 hr,
The mortality rate was examined after 48 hours. (See FIG. 7) In the figure, 23 is a pudding cup, 24 is a filter paper, and 25 is a rice seedling. (2) Test result

[Table 6] Experimental Example 6 Antibacterial activity test (1) Trichophyton was inoculated into an enrichment medium and cultured at 25 ° C. for 10 days, and then spores were added to 0.005% sodium dioctyl sulfosuccinate solution at about 10 ⁶ / ml. It was made to float and this was made into the bacterial solution. Then, this bacterial solution was added and mixed at a ratio of 1 ml to 150 ml of a measurement medium, and the mixture was dispensed in a petri dish in an amount of 15 ml to solidify to obtain a test medium. Pellets 1 microencapsulated with dextrin
One piece, three pieces, and five pieces were placed on the center of the test medium, sealed, and cultured at 25 ° C. for 14 days, and then the presence or absence of a growth inhibition zone around the pellet was visually determined. (2) Test result

[Table 7]

[0006]

As described above, since the insect-repellent antibacterial substance according to the present invention is prepared by dispersing the essential oil of moon peach in a solvent with a dispersant and then coating it with a coating agent so that it can be released continuously. Due to the enhanced physiological activity of peach (excellent insecticidal and antibacterial action by more than 100 natural compounds contained), it has antibacterial effect against microorganisms such as bacteria, fungi, actinomycetes, and diamondback moth larvae, The insecticidal effect against ticks, termites, etc., and the repellent effect against cockroaches, which have a strong vitality, are remarkably recognized, and as described above, since the peach essential oil is microencapsulated, long-term The sustainability of the effect can be realized,
It is possible to obtain extremely important operational effects in commercializing such products. In addition, the synergistic effect of 100 or more natural compounds contained in the above-mentioned moon peach essential oil extracted from plants has the characteristic that resistance to microorganisms and insects is less likely to occur. In addition to being possible, it is significantly safer than the currently widely used chemical synthetic fungicides and pesticides. Therefore, resins, resin moldings, paints, waxes, turfgrass regulators, pesticides and pharmaceuticals. It can be applied to various uses.

[Brief description of drawings]

FIG. 1 is an explanatory view showing an insecticidal activity test for adult cockroaches.

FIG. 2 is an explanatory view showing an insecticidal activity test for adult cockroaches.

FIG. 3 is an explanatory view showing an insecticidal action test for adult cockroaches.

FIG. 4 is an explanatory view showing an insecticidal activity test for adults of the mite mite.

FIG. 5 is an explanatory view showing an insecticidal activity test for adults of Tickworm.

FIG. 6 is an explanatory view showing an insecticidal activity test for diamondback moth larvae.

FIG. 7 is an explanatory diagram showing an insecticidal activity test for green leafhopper larvae.

[Explanation of symbols]

 1 Container 2 Lid 3 Saran Net 4 Pellets 5 Boxes 6 Boxes 7 Box Holes 8 Charles 9 Cockroaches 10 Petri dishes 11 Petri dishes 12 Kinaga Kodani 13 Japanese paper 14 Charles 15 Sponge 16 Filter paper 17 Tsurunashi green bean leaf 18 Filter 19 20 Pudding cup 21 Filter paper 22 Cabbage leaf 23 Pudding cup 24 Filter paper 25 Rice seedling

Claims (3)

[Claims] 1. An insect-repellent antibacterial substance obtained by dispersing ginseng essential oil in a solvent with a dispersant, and then coating the coating with a coating agent so that it can be released continuously. 2. The insect-repellent antibacterial substance according to claim 1, wherein the coating agent is an inclusion agent such as cyclodextrin. 3. The insect-repellent antibacterial substance according to claim 1, wherein the coating agent is a film-forming agent that forms microcapsules.