JPH05281189A - Slab-type electrical cataphoresis device - Google Patents

Abstract

PURPOSE:To end cataphoresis automatically under optimum conditions by providing a means for identifying end point for detecting the end point of a migration marker optically and a means for stopping current supply to a gel migration means based on information from the means for identifying the end point. CONSTITUTION:A buffer liquid 27 for upper migration and an anode electrode 25 are laid out within an upper buffer liquid bath 23. Also, a buffer liquid 28 for lower migration, a light source 23, and a color identification sensor 33 which receives light which is irradiated from the light source 23 are laid out within a lower buffer liquid bath 24. After a storage liquid of sol for separation which is mixed in a specified ratio is deaerated by an aspirator, it is poured into a gel storage part. After water is eliminated, a condensed gel liquid solution is poured onto the gel for separation, a gear-lock tool of the gel storage body 1 is loosened, the bottom plate is removed, and then the gel storage body 1 is mounted to a migration bath 22. When bromophenolblue (BPB) of the marker after migration reaches the lower edge of the gel for separation, wavelength from a light source 32 changes according to the marker BPB. The change is received by a sensor 33 and the current change automatically stops current supply.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a vertical slab type electrophoretic device, and more particularly, to a slab type electrophoretic device capable of automatically and accurately performing accurate fractionation in sample electrophoresis. Regarding the device.

[0002]

2. Description of the Related Art As is well known, the electrophoresis method is
The protein fractionation can be performed with excellent resolution even with a small amount of sample. Among them, the slab-type electrophoresis method (which refers to an electrophoresis method in which a gel is vertically or vertically run, the same applies hereinafter) is a disc type. Compared to the electrophoresis method, it is superior in operability and resolution, is particularly excellent in the large-scale fractionation of proteins, enzymes, and peptites, and is suitable for the separation of some nucleic acids and physiologically active substances that are difficult to heat-treat. It is also widely used in various fields of research.

By the way, in such an electrophoretic method, the electrophoretic time varies greatly depending on the type and amount of the sample to be electrophoresed or the electrophoretic conditions. Supply and stop must be done, and such work is very complicated,
If the electrophoretic marker runs off the sample, the amount of protein transfer in the sample cannot be calculated, so the work of supplying and stopping the current is extremely complicated and requires skill. Was there.

As a means for solving such a problem,
It is conceivable to connect a timer to the migration power supply and stop the current supply by this timer, but such a solution is effective when a known sample is migrated many times under the same conditions. However, there is a problem that this method cannot be used when the migration conditions are changed even a little such as when the sample is first migrated or when the gel composition is changed.

In addition, among the above-mentioned migration conditions, the temperature condition of the buffer solution in the migration tank is an important condition for determining migration conditions that do not inactivate the enzyme function.
When the temperature of the buffer solution is measured by a metal temperature sensor made of platinum, the platinum is likely to be electrolyzed during migration and easily dissolved in the buffer solution, so that accurate temperature measurement cannot be continuously performed. I also had the problem.

Further, in the above-mentioned conventional slab type electrophoresis method, a gel having a thickness of more than 1 cm is likely to drop due to its own weight, and in order to prevent this, the lower end portion of the gel is
For example, it may be supported by a net or the like, but when a protein or nucleic acid having a molecular weight of several hundred thousand units is electrophoresed, an appropriate gel concentration is, for example, 100% gel preparation solution 100.
The total number of grams of simple substance (acrylamide and Bis acrylamide) contained in ml (hereinafter simply referred to as T%) is
It is 5% or less by electrophoresis of polyacrylamide and 0.4% or less by agarose electrophoresis.

However, in the case of such a soft gel having a low gel strength and a slippery gel containing a surfactant such as SDS (sodium dodecylsulfate), the gel slides down before the completion of the electrophoresis to form a shape. It cannot be kept constant, or the electrophoretic band is slackened and the electrophoretic band smiles, or the gel peels off from the top and the sample leaks and electrophoresis cannot be performed. It had a problem that it was difficult to collect.

The present inventors have conducted various experiments to solve the above-mentioned various problems, and as a result, when a protein such as an enzyme is electrophoresed using a polyacrylamide gel as a gel for electrophoresis, a preceding marker for electrophoresis is obtained. Bromophenol Blue (hereinafter referred to simply as BPB
Say. ) Is used, it has been discovered that the movement of the BPB of the preceding marker can be easily detected by a color identification sensor.

The present invention was devised on the basis of such knowledge, and its purpose is to detect the migration state of a sample automatically without setting the migration conditions in advance and to optimize the optimum conditions. To provide a slab type electrophoretic device capable of automatically terminating the electrophoresis with, and continuously measuring the temperature of a buffer, which is important for determining the electrophoresis conditions that do not inactivate the enzyme function. To do.

Further, according to the present invention, even if the above-mentioned soft gel or slippery gel is used, the shape can be kept constant and the slack of the gel can be surely prevented. Another object of the present invention is to provide a slab type electrophoretic device using a gel holder capable of extremely easily purifying and collecting a target substance.

[0011]

In order to achieve the above object, according to the present invention, an end point identifying means for optically detecting an end point of an electrophoretic marker is provided in a slab type electrophoretic device, and this end point identifying means is provided. And means for stopping the current supply to the gel migration means based on the above information.

Further, in the present invention, since the temperature of the buffer solution is continuously measured, the temperature of the buffer solution in the migration tank in which the gel holder is immersed in the slab type electrophoresis apparatus is detected, It is characterized in that a means for automatically recording the detected temperature transition is provided. In the present invention, the temperature sensor for detecting the above-mentioned temperature is soaked in the buffer solution on the upper part of the gel in consideration of the highest temperature of the upper part of the gel and the easy operation. It is desirable to arrange the

Further, in the present invention, the gel is
From the viewpoint of preventing the self-weight drop of the vertically arranged gel,
The object is achieved by disposing a protrusion in the lower part of a gel storage part formed between plate bodies that sandwich the gel and storing the gel in the gel storage part. An electrophoretic device provided with such a gel protrusion is, for example, Japanese Patent Application No. 2-26 filed previously by the present applicant.
Details are given in No. 7598.

Further, according to the present invention, a plurality of small holes are formed in the lower portion of the gel container so that the shape of the soft gel or the slippery gel can be kept constant. Alternatively, it is characterized in that a stopper plate body for preventing gel drop having a plurality of slits is detachably mounted.

[0015]

BEST MODE FOR CARRYING OUT THE INVENTION The present invention will be described in detail below based on an embodiment shown in the accompanying drawings.

As shown in FIG. 1, a slab type electrophoretic device 20 according to this embodiment comprises a gel support 1, a stopper 21 for fixedly supporting the gel holder 1, a migration tank 22, and a migration tank 22. An upper buffer solution tank 23 formed on the upper part, a lower buffer solution tank 24 formed on the lower part of the migration tank 22,
Anode electrode 25 disposed in the upper buffer tank 23
A cathode electrode 26 disposed in the lower buffer solution tank 24, an upper migration buffer solution 27 accommodated in the upper buffer solution tank 23, and a lower electrode accommodated in the lower buffer solution tank 24. A side migration buffer solution 28, a migration power supply 29, a temperature sensor 30 arranged in the upper buffer solution tank 23, and a recorder 31 for recording the transition of the temperature detected by the temperature sensor 30. Light source 3 disposed in the lower buffer tank 24
2, a color identification sensor 33 that receives the light emitted from the light source 32, and a switch that recognizes a change in the light amount conversion voltage or a specific wavelength received by the color identification sensor 33 and stops the supply of current ( (Not shown). In the figure, reference numeral 34 is a seal packing, 35 is an electrophoretic gel, and 36 is a control device for supplying / stopping an electric current and controlling the temperature. In the present invention, the light source 32 does not necessarily have to be provided in a room where normal illuminance can be secured, but it is provided because it cannot perform stable detection in a dark room or a place where the illuminance is likely to change. Is desirable.

As shown in FIG. 2, the gel holder 1 has a transparent acrylic resin plate 2 having a side of about 19 cm and a thickness of about 1.0 cm, and the same acrylic resin plate 2. And a transparent acrylic resin plate 3 having a U-shaped notch 4 at its upper end and having side plates 6 and 7 protruding from both sides of the acrylic resin plate 2. A width dimension of about 2.0 cm is formed between the acrylic resin plates 2 and 3. The acrylic resin plate 2,
As shown in FIG. 2, screw 3 is screwed into screw holes 6a and 7a formed in side plates 6 and 7 of acrylic resin plate 2 and is integrated by tightening them.

The lower end portion 8 of the gel storage portion 10 formed between the acrylic resin plates 2 and 3 of the gel holder 1 is a removable acrylic bottom plate 5 having a packing on one side surface. It is configured so that it can be sealed.

As shown in FIGS. 2 and 3, on the bottom plate 5, there are provided two upper and lower locking members 11 made up of known corner catch clips and the like, respectively. 11 is obtained by engaging the ring 11a with the hooks 2a and 3a protruding from the surface side of the acrylic resin plates 2 and 3,
The locked state is configured to be locked by rotating the actuating body 11b connected to the ring 11a. The hooks 2a and 3a and the screw B are either ceramic-coated or arranged at a position where they are not immersed in the lower migration buffer solution 28.

Further, gel stoppers 15, 16, 17, 18 which are projections for locking and holding the gel are formed below the insides of the both side portions 6, 7.

Each of these gel stoppers 15 to 18 is
Although it is shown in FIG. 1 as having a substantially triangular cross section, the present invention is not limited to this, and a shape capable of preventing the gel from falling downward, for example, a U-shaped cross section or a cross section. A semicircular shape, a continuous wavy shape, a mesh shape, or any other appropriate shape can be applied. Further, it is desirable that the overhanging dimensions of each of the gel stoppers 15 to 18 be appropriately set and changed according to the total amount of the separating gel determined by the width dimension of the bottom plate 5. At this time, the number of gel stoppers can also be set to an appropriate number according to the total amount of the separating gel.

As shown in FIG. 2, according to the gel holder 1, 1.5 cm (a maximum of 5 cm or more is possible, but in the case of 2 cm or more, a plurality of gel holders are stacked to form a unit. It is also possible to prepare a gel), and the composition of the buffer solution or gel solution used is to be prepared according to the so-called Davis method.

The temperature sensor 30 is made of a material that is not electrolyzed even when immersed in a buffer solution during migration. For example, it is desirable to cover the temperature detecting portion with glass or the like.

The temperature detected by the temperature sensor 30 is automatically recorded in the recorder 31 in the form of a numerical value or a line graph. Since the recorder 31 has the same structure as a known recorder, its detailed description is omitted here.

The light source 32 and the color identification sensor 33 are attached to the opposing wall surfaces of the lower buffer tank 24 made of a light-transmissive material, and are placed opposite to each other at a position where light is not blocked in the middle.

As the color identification sensor 33, a known light receiving element corresponding to the material used as the electrophoretic marker is used.

The control unit 36 is composed of a well-known microprocessor unit (MPU), recognizes a change in the light quantity conversion voltage received by the color identification sensor 33 or a specific wavelength, and presets a measurement value. It is configured to stop the current supply from the power supply 29 by detecting the timing when the voltage value or the wavelength value is reached. Further, the control device 36 may be configured to automatically control heat generation of a heating unit (not shown) based on the temperature information measured by the temperature sensor 30 so that the migration is performed at a preset temperature with time. In this case, the electrophoresis is carried out at a low temperature of 0 ° C to 4 ° C in order to prevent the inactivation of the enzyme.

The details and operation of each of the components other than those described above are the same as those of the corresponding components constituting the known slab type electrophoretic device, unless otherwise specified. The detailed description is omitted here.

Next, in the case of separating and purifying dehydrogenase using the gel holder 1 having the above-described structure, first, the gel holder 1 is set on the gel manufacturing table. At this time, the preservation solution of the separating gel is mixed at a predetermined ratio.

After degassing this mixed solution with an aspirator,
It is poured into the gel storage unit 10 so that no bubbles are generated. At this time, the height of the gel is about 13 cm. Water is gently layered (height: about 2 mm), and then immersed in warm water at 30 ° C. for about 60 minutes to cause gelation.

Then, after removing the water, the concentrated gel solution is poured onto the separating gel to completely gel the gel, and then the locking tool 11 at the lower part of the gel holder 1 is loosened to remove the bottom plate 5. ,
As shown in FIG. 1, it is attached to the migration tank 22 via a stopper 21.

Next, the migration buffer solutions 27 and 28 are filled in the buffer solution tanks 23 and 24 at the upper and lower portions. About 10% in enzyme sample solution
Glycerin, a concentrated gel buffer, and a few drops of BPB, which is an electrophoretic marker, are added in appropriate amounts and mixed. Of course, in the present invention, when electrophoresis is performed in the acidic region, a known marker such as methyl green can be appropriately used as a migration marker. When the electrophoresis is performed in the acidic region, the anode electrode 25 and the cathode electrode 26 are reversed.

The sample solution thus obtained is gently overlaid on the concentrating gel using a pipette or an arterial pump. The sample that can be added at one time is 30 to 120 ml, and the height of the sample liquid tank is about 3 cm. When the amount of the sample is large, it is possible to perform electrophoresis for a certain period of time, and when the sample enters the concentrated gel to some extent, 20 to 50 ml of the sample solution can be repeatedly added.

The marker BPB is electrophoresed at a current of about 50 mA until it enters the separation gel, and then at a current of 60 to 100 mA for several hours.

After the electrophoresis, when the marker BPB reaches the position of 30 mm from the lower end of the separating gel, the wavelength from the light source 32 is changed by the marker BPB, and the color discrimination sensor 33.
The changed light is received by. The voltage change at this time is
Since the voltage is about 500 mV, the control device 36 can clearly discriminate the changed state and automatically stop the current supply from the power source 29 to the anode electrode 25 and the cathode electrode 26, so that the migration is optimal. It is automatically stopped in the state. Of course, in the present invention, the means for stopping the current supply from the power supply 29 is not to identify the quantitative change of the light quantity conversion voltage, but to supply the current by the wavelength balance changed by the marker BPB, for example. Even if it is configured to stop, the same effect can be obtained. It should be noted that when the supply of the current is automatically stopped, for example, a lamp may be blinked or a buzzer may be sounded.

On the other hand, in the slab type electrophoretic device 20 according to the present embodiment, the temperature sensor 30 measures the migration temperature over time, and the heating means (not shown) is used based on the temperature information measured by the temperature sensor 30. It is also possible to automatically control the heat generation.

After that, the gel holder 1 is quickly removed from the slab type electrophoresis apparatus 20 according to this embodiment, and then the acrylic resin plate 3 on one side of the electrophoresis plate is removed, and one end of the separating gel is about 5 mm. Cut at the width of. Then, this gel fragment was placed in a glass tray,
Tris-HCl buffer (pH 8.0), 0.3 mM NAD
(Nicotinamido Adenin Dinucleotide), 0.8 mM
Substrate, 0.2 mM phenazine methosulfate (Phen
azine methosulfate), 0.8 mM MTT (Dimethyt
Soak in 50 ml of reaction solution containing hiazol tetrazolium) for 3
Perform activity staining for several minutes at 7 ° C to 50 ° C. When a red-purple active band appears, take out the gel piece, return it to the original gel position, and horizontally cut out the gel at the active band position slightly wider than the band width. The other part is removed, leaving only the part of the linear active band that appeared.

Next, the active dyed gel was finely cut (a rectangular shape with a width of about 5 mm), and a potter type Teflon homogenizer (volume: 50 m) was used together with a buffer solution (pH 7.2).
Grind with 1) while cooling with ice. Then, the gel grinding liquid is centrifuged (20,000 × g, 10 minutes, 4 ° C.) to obtain an enzyme extract in the supernatant. In addition, a small amount of buffer solution is added to the precipitated gel to suspend it, and then it is centrifuged again to obtain a second enzyme extract. According to such a preparation method, not only dehydrogenase but also many other enzymes capable of activity staining such as NAD and NADP can be purified. In addition, the Rf value can be similarly obtained and applied to purification without activity staining. In particular, it is a good separation means for the purification of an enzyme having a high thermostability such as an enzyme of a thermophilic bacterium.

FIGS. 4 and 5 show another example of the gel holder 1 used in the slab type electrophoretic device according to the above embodiment. In the structure of the gel holder 1A according to this example, the same components as those of the gel holder 1 shown in FIGS. 2 and 3 are designated by the same reference numerals as those used in FIGS. 2 and 3. Therefore, the detailed description thereof is omitted here.

That is, the gel holder 1A according to this embodiment
Have a thickness of 1. as in the gel holder 1 shown in FIGS.
A transparent acrylic resin plate 2 of about 0 cm and a transparent acrylic resin plate 3 having a U-shaped notch 4 at the upper end thereof.
And the side plates 6 and 7 are provided on both sides of the acrylic resin plate 2.
To project about 2.0 between the acrylic resin plates 2 and 3.
A width dimension of cm is formed, and the lower end portion 8 of the gel storage portion 10 formed between the acrylic resin plates 2 and 3 is detachably attached with packing on one side surface. It is constructed so that it can be hermetically sealed with an acrylic bottom plate 5, and a stopper plate body 19 having a plurality of small holes 19a penetrating is provided above the bottom plate 5 with screws 1.
It is detachably attached via 9b. Of course, the means for fixing the stopper plate body 19 is not limited to the case of using the screw 19b, but the side plates 6, 7
The slits may be opened at required intervals in the facing surface portion of and the stopper plate body 19 may be inserted into and fixed to the slits.

The stopper plate 19 is made of, for example, acrylic, Teflon, Duracon, or the like. Since a space must be provided in the bottom plate 5 in order to pass an electric current through the gel, the stopper plate 19 is formed. The area of the body 19 is preferably 10 to 95% or less of the opening area closed by the bottom plate 5. Note that a metal material that can be electrolyzed cannot be used for the stopper plate body 19.

The number of the small holes 19a is not limited to the six shown in the figure, but an appropriate number can be provided, and the shape thereof is not limited to the circular shape, and the gel thickness is not limited. In correspondence with, for example, as shown in FIG.
A plurality of slits 19c may be used, or notches may be formed at both ends of the stopper plate body 19.

The stopper plate body 19 thus constructed
Is preferably set between the lower end of the gel holder 1A and the intermediate height position.

In the above embodiment, the case where the distance between the gel storage portions 10 is 2.0 cm or more has been described as an example.
In the present invention, sufficient effects can be obtained even if the space between the gel storage portions 10 is set to 5 mm or more.

Next, the following experiments were conducted using the gel holders 1 and 1A having the above-mentioned structure.

[0046]

Experimental Example 1 As a sample, Catalase (Micrococcus Iysodeikticus) manufactured by Nagase Seikagaku Co., Ltd. was used.
A 60% glycerol solution containing 0.1% BPB was added to the sample enzyme solution, and the mixture was subjected to slab type polyacrylamide gel electrophoresis according to a conventional method. A gel having a thickness of 20 mm, which is not subjected to gel drop prevention treatment (hereinafter referred to as method A), a gel held by the gel holder 1 shown in FIGS. 2 and 3 (hereinafter referred to as method B), and a gel. What is held by the holding body 1A (hereinafter, referred to as C method) and what is held by the gel using a tacho thread net having a mesh size of 1 cm instead of the gel holding body 1A (hereinafter, referred to as D method). Street experiments were conducted in parallel. The gel concentration at this time is
Separation gel T% = 4.8%, C% which is the ratio of the number of grams of Bis acrylamide to the total number of grams of the used simple substance
Is 2.7%, the concentrated gel is T% = 3.1% and C% = 20.
It was set to 0%. Electrophoresis was carried out in a refrigerating room at room temperature of 5 degrees Celsius with a current of 3
0 mA for 2 hours, current 35 mA for 12 hours, current 50 m
A total of 39 hours of 25 hours. After the electrophoresis was completed, a part of the gel was cut out, and 0.1 M phosphate buffer (pH 7.
0) and the gel was removed by suction filtration. About the obtained extract, using 30% hydrogen peroxide as a substrate, OD
Enzyme activity was measured by measuring the change in hydrogen peroxide at (wavelength) 240 nm. The “flowing state of gel during electrophoresis” thus obtained is shown in Table 1 and
The “enzyme recovery rate by the stopper plate” is shown in Table 2.

[Table 1]

[Table 2] As is clear from the above results, the separation pattern of electrophoresis by the above-mentioned method C was comparable to the separation pattern of the analytical electrophoresis apparatus.

[0047]

[Experimental Example 2] As a sample, Al produced by Oriental Yeast Co., Ltd.
Cohol Dehydrogenase was used. Prepare the sample enzyme solution,
Slab-type N-methylol acrylamide gel electrophoresis was performed according to a conventional method. A gel having a thickness of 20 mm, which is not subjected to gel drop prevention treatment (hereinafter referred to as E method), and a gel which is held by the gel holder 1 shown in FIGS.
Called a method. ), A gel held by a gel holder 1A (hereinafter referred to as G method) and a gel held by a nylon net having a mesh size of 1 cm in place of the gel holder 1A (hereinafter referred to as H method). 4 experiments were conducted in parallel. The gel concentration at this time is T
% = 4.5%, Bi with respect to the total number of single grams used
C%, which is the gram ratio of s-acrylamide, is 2.7%,
The concentrated gel had T% = 3.1% and C% = 20.0%.
The separating gel consisted of 135 ml of A liquid, 81 ml of C liquid, 306 ml of water, 0.54 g of polyethylene glycol, 18.0 ml of E liquid as a polymerizing agent, N, N,
N ', N'-tetramethylethyleneamine (hereinafter, TEMED
Abbreviated) was added to 0.27 ml.

[Table 3] Further, the concentrated gel was prepared by adding 5.0 ml of solution B and D shown in Table 3 above.
Liquid 10.0 ml, water 24.0 ml, polyethylene glycol 0.04 g, liquid E 1.0 ml, and TEMED
It was produced by adding 0.007 ml. Electrophoresis was carried out in a refrigerator at room temperature of 5 degrees Celsius at a current of 50 mA for 1.3 hours and a current of 20.
It was carried out for a total of 20.8 hours at a current of 30 mA for 4.7 hours and a current of 30 mA for 14.8 hours. From a part of the gel after electrophoresis,
The target enzyme was extracted with 0.1 M phosphate buffer (pH 7.0), and the NADH production rate was measured at OD (wavelength) 340 nm to measure the enzyme activity. The "gel flow-down state during electrophoresis" thus obtained is shown in Table 4, and the "enzyme recovery rate by the stopper plate" is shown in Table 5.

[Table 4]

[Table 5] As is clear from the above results, the separation pattern of electrophoresis by the G method was comparable to the separation pattern of the analytical electrophoresis apparatus.

Therefore, the gel holder 1A according to this embodiment is
In the case of using SDS, the protein is denatured to make the charge uniform, the ease degree is determined only by the size of the molecular weight of the protein, and the molecular weight is estimated from the relationship between the weight degree and the SDS. Use a slippery gel containing the surfactant of
Alternatively, even if the soft gel is used, the shape of the gel can be kept constant, so
Collection can be easily performed.

Further, according to the present embodiment, it is not necessary to increase the current to carry out the electrophoresis in order to finish the electrophoresis within a short time before the gel falls off. Therefore, the gel heats up and the target substance is denatured. There is no fear of this, and the effect that it is suitable for fractionation of the target substance is also obtained.

[0050]

As described above, according to the slab type electrophoresis apparatus according to the present invention, the migration state of the sample is automatically detected and the migration is performed under the optimum conditions without setting the measurement conditions in advance. Can be automatically terminated, and the temperature of the buffer, which is important in determining electrophoretic conditions that do not inactivate the enzyme function, can be continuously measured. A large amount of physiologically active substances can be collected and purified at one time.

By attaching a stopper plate to the gel holder, the shape of the gel can be kept constant even if it is a soft gel or a slippery gel, so that the target substance can be securely treated without denaturation. It has a number of excellent effects such as the ability to purify and separate.

[Brief description of drawings]

FIG. 1 is a sectional view schematically showing a configuration of a slab type electrophoretic device according to an embodiment of the present invention.

FIG. 2 is an exploded perspective view showing a configuration of a gel holder.

FIG. 3 is a side sectional view of the gel holder.

FIG. 4 is an exploded perspective view showing another configuration example of the gel holder.

FIG. 5 is a side sectional view of the gel holder.

FIG. 6 is a perspective view showing another configuration example of a stopper plate body.

[Explanation of symbols]

 1, 1A Gel holder 19 Stopper plate 19a Small hole 20 Slab type electrophoretic device 30 Temperature sensor 31 Recorder 32 Light source 33 Color discrimination sensor 36 Control device

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Tomohiko Fukuda 7-90 Aoyama-cho, Handa-shi, Aichi Prefecture Aoyama Corp. E (72) Inventor Masahiro Fujimori 2-17, Horizaki-cho, Handa-shi, Aichi Corp Nomura Hanada 2-304 Issue (72) Inventor Yoshiya Kawamura 132 Furutawa, Kochino-cho, Konan-shi, Aichi

Claims (4)

[Claims] 1. An end point identifying means for optically detecting an end point of an electrophoretic marker, and a means for stopping current supply to the gel electrophoretic means based on information from the end point identifying means. Slab-type electrophoresis device. 2. A slab-type electrophoresis apparatus configured to detect the temperature of a buffer solution in a migration tank in which the gel holder is immersed and automatically record the detected temperature transition. 3. The gel is stored in a gel storage portion formed between plate bodies sandwiching the gel, and a protrusion for preventing the gel from falling is formed in a lower portion of the gel storage portion. The slab type electrophoretic device according to claim 1 or 2, wherein 4. The gel is stored in a gel storage part formed between plates holding the gel, and a plurality of small holes or a plurality of slits are formed in the lower part of the gel storage part. The slab type electrophoretic device according to any one of claims 1 to 3, wherein the gel drop preventing stopper plate is detachably attached.