JPH0535328Y2 - - Google Patents

Description

[Detailed Description of the Invention] [Industrial Application Field] This invention relates to an electrophoresis device used in electrophoresis.

[Conventional technology]

Electrodynamics involves connecting two containers containing electrolyte solutions with a separation gel, introducing a sample for analysis (various proteins, etc.) into one container, and inserting electrodes into the two electrolyte solutions. In this method, a direct current voltage is applied to charge and migrate sample particles, and the components of these particles are analyzed.

The conventional electrophoresis apparatus used in the above electrophoresis has a structure as shown in FIG. That is, a container A containing an alkaline solution (e.g., sodium hydroxide solution) is placed on one side, and a container B containing an acidic solution (e.g., a phosphoric acid solution) is placed on the other side.
These containers are connected by a separation gel C, and the electrolyte is in contact with the gel C via a porous filter D. Then, sample E is added to container A containing the alkaline solution using a microsyringe or the like.
is introduced, a DC voltage is applied, the charged sample E is oozed into a separation gel C, electrophoresed, and analyzed.

[Problem that the invention attempts to solve]

In the conventional electrophoresis apparatus as described above, the separation gel and the sample in the container are always in contact with each other via a filter. Therefore, since the separation gel and the sample are in contact for the entire electrophoresis time, there are problems in that tailing often occurs in the analysis results and that efficient separation cannot be performed in a short time. Furthermore, the above-mentioned apparatus currently in use has a problem in that once sampling is performed, the gel and sample cannot be completely separated.

This invention was made in view of the above problems, and its purpose is to solve the above problems by making it possible to separate the separation gel and the sample at any time.

[Means for solving problems]

That is, in order to solve the above-mentioned problems, this invention consists of two containers, one containing an alkaline electrolytic solution and the other containing an acidic electrolytic solution, a separation gel connecting these containers, and a separation gel that connects these containers. In an electrophoresis apparatus consisting of a DC power supply with a cathode and an anode placed in electrolytic solutions in two containers, one of the containers is separated into a plurality of chambers by a partition wall, one of which is used as a sample chamber, and the plurality of It is characterized by having switchable electrodes within the same electrode placed in each separate room.

[Effect]

When a direct current voltage is applied to the electrodes in the chamber containing the sample, the sample becomes electrically charged and oozes into the separation gel. Next, at an appropriate time, the electrodes are switched to a room that does not contain a sample, and the sample is placed outside the current of electricity and the sample in the container no longer enters the separation gel. Therefore, subsequent electrophoresis within the separation gel can be performed separately from the sample in the container.

〔Example〕

Hereinafter, specific embodiments of this invention will be described with reference to the drawings.

FIG. 1 is a longitudinal sectional side view of the electrophoresis apparatus according to this invention on the side of a container into which a sample is introduced. In this diagram 1
2 is a container containing an electrolyte solution, and 2 is an electrolyte solution placed in this container 1, which contains, for example, an alkaline solution of sodium hydroxide. 3 is an electrode (-pole),
Reference numeral 3' is also an electrode (-pole), which can be arbitrarily switched to either the terminal 31 of the electrode 3 or the terminal 32 of the electrode 3' by means of a rotary contactor 30. Reference numeral 4 denotes a partition wall for partitioning the container 1 into two chambers 12 and 13. As shown in this figure, the chamber 13 is shaped like a crank or a labyrinth, and the chamber 12 and the filter 5 are separated from each other at the bottom of the partition wall 4.
connected to. 6 is a separation gel, and this gel 6 is made of polyacrylamide, for example.
7 is the crank-shaped or labyrinth-shaped room 1
This is an analysis sample introduced into the lower part of 3 with a microsyringe 14. This analysis sample includes, for example, a mixture of various proteins.

FIG. 2 is an overall schematic diagram of the electrophoresis apparatus according to this invention. In this figure, 8 is a container containing an acidic electrolytic solution 9, and the acidic solution 9 is, for example, a phosphoric acid solution. Further, an electrode (+ electrode) 10 is placed in the solution in the container 8 and connected to the electrode 3 or 3' of the container 1 via a power source 11. Further, the container 8 is connected to the container 1 via a separation gel 6. That is, the separation gel 6
One is an alkaline solution and the other is an acidic solution, and a DC voltage is applied as a driving source when the sample is electrophoresed on the separation gel 6. 15 is a cooling plate installed under the separation gel 6.

As shown in FIGS. 1 and 2, the electrolytic solution 2 is in contact with the gel 6 through the porous filter 5 as in the conventional case, but the container 1 containing the electrolytic solution 2 is The room is divided into two, 12 and 13, and the sample is also introduced into room 13. When a sample is introduced into the separating gel 6, the rotary contactor 30 is brought into contact with the terminal 32 of the electrode 3' placed in the chamber 13 on the sample side, and a DC voltage is applied. When a direct current is applied in this manner, the sample becomes electrically charged and exits from the filter 5 and migrates within the separation gel 6, where it is separated into components unique to each protein. Furthermore, when the rotating contactor 30 is switched from electrode 3' to electrode 3 at an appropriate time, the current flows as shown by the broken line, and the sample 7 in the container is placed outside the flow of electricity and comes out of the chamber 13. I can't do anything. Therefore, in electrophoresis after switching from electrode 3' to electrode 3, electrophoresis can be performed completely separately from the sample.

Note that the chambers 12 and 1 of the container 1 are separated by a partition wall 4.
It is appropriate that the capacity of room 12 is about 20 ml to 50 ml, and the capacity of room 13 is about 5 ml to 10 ml.

[Effect of idea]

Since the electrophoresis apparatus according to this invention has the configuration described in detail above, it is possible to perform electrophoresis completely separated from the sample after a certain point in electrophoresis. As a result, the sample can be efficiently introduced into the gel in a short time, and tailing can be prevented and accurate analysis results can be obtained.

[Brief explanation of the drawing]

Fig. 1 is a longitudinal sectional view of the sampling port portion of the electrophoresis device according to this invention, Fig. 2 is an overall schematic diagram of the electrophoresis device according to this invention, and Fig. 3 is a schematic diagram of a conventional electrophoresis device. be. 1...Container for electrolyte solution, 2...Electrolyte solution (alkaline), 3,3'...electrode (-electrode), 4...
Partition wall, 5...Porous filter, 6...Separation gel, 7...Sample, 8...Container for electrolyte solution, 9...
... Electrolyte solution (acidic), 10 ... Electrode (+ electrode), 1
1... DC power supply, 12, 13... Partition room, 3
0... Rotating contact, 31, 32... Terminal.

Claims (1)

[Scope of utility model registration request] Two containers containing an alkaline electrolytic solution in one and an acidic electrolytic solution in the other, a separation gel connecting these containers, and a direct current containing a cathode and an anode in the electrolytic solutions in the two containers, respectively. In an electrophoresis apparatus comprising a power source, one of the containers is separated into a plurality of chambers by a partition wall, one of which is used as a sample chamber, and each of the plurality of separated chambers is provided with an electrode that can be switched within the same electrode. An electrophoresis device characterized by containing.