JPH0540085A - Bio particle measuring device - Google Patents

Abstract

(57) [Summary] [Purpose] Measuring many kinds of test items such as red blood cell count, white blood cell count, white blood cell classification and reticulocyte of particle suspension with high throughput, and at that time, a small amount of necessary samples and reagents are measured. It is possible. [Structure] A quantitative sampling valve 101 for quantifying a sample, syringes 104 to 110 for aspirating and discharging various reagents and samples, sample ports 115 to 118 for supplying various reagents and samples to a pipettor, and a chamber for stirring and staining Pipettes 201-204,
Syringes 205 to 208 for driving the pipettor, fluorescence detecting flow cell 301, blood cell counting flow cell 30
2, 303 are the main constituent elements.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a particle measuring device,
In particular, the present invention relates to a bioparticle measuring apparatus suitable for analyzing particles such as blood or increasing the measurement efficiency of inorganic fine particles in a fluid and increasing versatility.

[0002]

2. Description of the Related Art As a conventional biological particle measuring apparatus, there is an "automatic blood analyzer" disclosed in Japanese Patent Publication No. 59-16667. Most of automatic blood analyzers capable of measuring a plurality of items, as represented by this prior art, have a fluid circuit including a pipe, a container for measurement and agitation, and a solenoid valve for controlling the flow therein. It is composed by. This device is a device for counting blood cells, a system for performing two-step dilution for counting red blood cells, a system for performing hemolysis and dilution for counting white blood cells, and a system for performing diluted hemolysis for measuring hemoglobin. And are provided. Therefore, many tubes are complicatedly arranged for the dilution stirring chamber and sample reagent transportation.

[0003]

In the prior art, since the sample and the sample are sequentially moved from container to container through the tube, one sample occupies the flow path system, and the throughput (per unit time) is increased. However, there was a drawback that the number of samples processed was poor. Further, the above-mentioned conventional technique requires a large amount of the sample and the reagent because the previous sample and the sample attached to many containers and tubes are washed together with the subsequent sample. In particular, the sample blood could be wasted.
Further, the functions of the five classifications of white blood cells are very different from each other in the test method, and thus it is difficult to install the functions in the conventional blood test machine as they are.

An object of the present invention is to measure many kinds of test items with high throughput in particle measurement such as blood cell measurement, and at the same time, a small amount of sample and reagent is required for the biological particle measuring apparatus. To provide.

[0005]

To achieve the above object, a biological particle measuring apparatus according to the present invention is a biological particle measuring apparatus equipped with a particle measuring section, which is a quantitative sampling apparatus for quantitatively collecting a particle suspension. And at least one transportation means for transporting the quantitatively collected particle suspension, at least one connecting portion for connecting the transported particle suspension, and the particle suspension from each of the connecting portions. A configuration is provided that includes at least one pipettor that aspirates and agitates the particle suspension, and a particle measurement unit that has at least one flow cell that contracts the particle suspension discharged from each pipettor.

In a biological particle measuring device equipped with a particle measuring unit, a quantitative sampling device for quantitatively sampling a particle suspension, and a reagent for dilution and reaction are supplied and transported to the quantitatively sampled particle suspension. At least one reagent supplying means, at least one connecting portion for connecting the transported particle suspension, and at least stirring the particle suspension by sucking the particle suspension from each connecting portion. A configuration including one pipettor and a particle measuring unit having at least one flow cell that contracts the particle suspension discharged from each pipetter may be used.

Further, at least one flow cell may be a sheath flow cell having a constriction part for constricting the particle suspension and a thin tube part in which particles of the particle suspension are aligned and flow. ..

Further, at least one flow cell has a constriction part for constricting the particle suspension and pores in which the particles of the particle suspension are aligned and flow, and have a potential difference between front and rear. It may be a configuration of an electric resistance type cell in which particles are passed through pores and the properties of the particles are measured.

The quantitative sampling device is equipped with a quantitative sampling valve for quantitatively quantifying a part of the first flow path filled with the collected particle suspension by switching to a part of the second flow path. You may have a structure.

Further, each pipettor may be provided with a reaction vessel for mixing the particle suspension with a reagent and reacting them.

Further, a plurality of quantitative sampling devices and a plurality of particle measuring units may be provided to simultaneously measure a plurality of particle suspensions.

A plurality of quantitative sampling devices and a plurality of particle measuring units are provided, and at least one distributing / supplying unit for distributing and supplying the same particle suspension to each particle measuring unit is provided. A configuration for measuring properties may be used.

Further, in the biological particle measuring device having the particle measuring unit, the particle suspension is sucked into the first flow path so that a plurality of parts of the first flow path become a part of the other plurality of flow paths. A plurality of quantitative sampling valves that perform a large number of quantitative determinations at once by switching each, and a plurality of reagent supply means that supplies a plurality of reagents to each of a plurality of flow paths and transports together with the quantitatively sampled particle suspension. A plurality of connection parts for connecting the transported particle suspension, a plurality of pipettors for agitating the particle suspension by aspirating the particle suspension from each connection part, and a plurality of pipettes for discharging the particle suspension. Alternatively, a configuration including a plurality of particle measuring units having a plurality of flow cells that contract the particle suspension may be used.

Further, the particle suspension once quantified is transported together with the reagent to a predetermined container, and after mixing and reacting, it is also equipped with a two-step reaction means for sucking again into the quantitative sampling valve and quantifying again. Good.

[0015]

According to the present invention, in the quantitative sampling valve, a part of the first flow path filled with the collected suspension is switched to a part of the second flow path to carry out the quantitative measurement.
The quantified particle suspension is transported to a predetermined connection portion through a second flow path that is a reagent supply means for supplying a reagent for dilution or reaction.

Next, a pipettor is connected to the connecting portion to suck the particle suspension. The stirring reaction of the particle suspension is carried out in the reaction tank inside the pipettor.

Next, the pipette is connected to the suspension supply port of the flow cell of the particle measuring unit to discharge the particle suspension to the particle measuring unit to form a contracted flow and measure the particle suspension.

Further, in the quantitative sampling valve,
The particle suspension is sucked into the first flow path, and a plurality of portions of the first flow path are switched to a part of the other plurality of flow paths to perform a large number of quantitative determinations at a time and to obtain a plurality of flow paths. Multiple reagents are separately supplied to the channels, the quantified particle suspension is transported together with the reagents to multiple connections, and the quantitative suspension and reagents are mixed and reacted by multiple pipettes, and then the particles are measured in the particle measurement section. A large number of measurement items are simultaneously measured for one type of sample by transporting and measuring them in a plurality of measuring sections.

[0019]

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS An embodiment of the present invention will be described with reference to FIGS.

1 to 3 are basic configuration diagrams of the apparatus of this embodiment. This apparatus includes a reagent supply unit 100 and a stirring dyeing unit 2
00 and the particle measuring unit 300.

The reagent supply section 100 includes a quantitative sampling valve 101 which constitutes a quantitative sampling device for quantitatively measuring a particle suspension (sample), a sample sampling device 102 which sucks a sample from a sampling tube, and a suction and discharge of a leukocyte staining reagent. Leukocyte Staining Reagent Syringe 104 that is a Transportation Means
And a reticulocyte staining reagent syringe 105 that sucks and discharges a reticulocyte staining reagent, and a one-step diluent sucking syringe 106 that sucks a one-step diluent into a sampling valve.
And a two-stage dilution liquid syringe 107 for sucking and discharging the two-stage dilution liquid, and a suction and discharging for the one-stage dilution liquid 1
A serial diluent syringe 108, a leukocyte counting reagent syringe 109 that sucks and discharges a leukocyte counting reagent, a sample suction syringe 110 that sucks a sample into a sampling valve, a leukocyte staining reagent 111, and a reticulocyte staining reagent 112. , Diluent 113, white blood cell counting reagent 114
And a leukocyte staining sample port (connection part) 115 for supplying the leukocyte staining sample to the pipettor, a reticulocyte staining sample port 116 for supplying the reticulocyte staining sample to the pipettor, and a diluted sample for supplying the diluted sample to the pipettor Sample port 117, a white blood cell counting sample port 118 for supplying a white blood cell counting sample to the pipettor, a staining pipettor washing tank 119 for washing the staining pipettor, and a red blood cell counting pipetter for washing the red blood cell counting pipetter. A washing tank 120, a leukocyte counting pipette washing tank 121 for washing the white blood cell counting pipettor, a first-stage dilution stirring chamber 122 for stirring the first-stage diluted solution, and leukocyte staining reagent transport tubes 128, 135, reticulated Red blood cell staining reagent transport tubes 129, 136, Stage dilution liquid transport tube 132,13
8, 140, 130, two-stage dilution liquid transport tube 131,
137, leukocyte counting reagent transport tubes 133 and 139
And sample transport tubes 134 and 146.

The quantitative sampling valve 101 includes a leukocyte staining sample collecting section 123, a one-step dilution sample collecting section 124, a leukocyte counting sample collecting section 125, a two-step dilution sample collecting section 126, and a reticulocyte staining sample collecting section. 127 and sample tube washing parts 141, 14
2, 143, 144, 145.

The stirring / staining unit 200 operates a white blood cell staining pipetter 201, a reticulocyte red blood cell staining pipettor 202, a red blood cell counting pipettor 203, a white blood cell counting pipettor 204, and a working fluid 211 which are provided with a reaction tank (chamber) for stirring. , A leukocyte staining syringe 205 that drives the pipettor, a reticulocyte staining syringe 206, a red blood cell counting syringe 207, a leukocyte counting syringe 208, a staining pipettor fixing disk 209, and an agitating pipettor fixing disk 210. ..

The particle measuring unit 300 comprises a flow cell 301 for measuring white blood cells and reticulocytes, and a flow cell 3 for counting red blood cells.
02, a white blood cell counting flow cell 303, a laser light source 304 for irradiating a sample with a laser beam, a fluorescence detection device 305 for detecting fluorescence from the sample, an insulating chamber 306,
It is composed of a counting signal processing device 307, an electrode 308, and a sample passage hole 309.

Further, the present apparatus includes a compressor 310 for supplying a cleaning liquid to each part, a cleaning liquid bottle 311 which receives the pressure of the compressor 310 and sends the cleaning liquid to each part,
There is a waste liquid suction bottle 312 for sucking the waste liquid.

Next, a pipettor equipped with a reaction tank (hereinafter referred to as a direct mixing pipetter) will be described with reference to FIG. FIG. 4 shows an example of the structure of a direct mixing pipettor.

A direct mixing pipettor, for example, a leukocyte staining pipettor 201, has a cross-sectional area that is tapered and expanded from the top to the center, and has a cylindrical shape from the center to the bottom, and the part connecting the bottom and the pipe is The pipette pipe 401 is provided with a microchamber (reaction tank) 402 which is curved so as to be smoothly connected. Normally, the direct mixing pipettor is filled with the hydraulic fluid 211. The sample sucked through the pipettor pipe is swirled by the action of the hydraulic fluid to generate a vortex flow at the sudden expansion and contraction portions in the microchamber and is fluidly stirred. Since a very small amount of liquid can be stirred in the microchamber, it is possible to reduce the amount of sample and reagent.

Next, an example of the apparatus configuration of the dyeing section using the direct mixing pipettor will be described with reference to FIG. Figure 5
Is an example of a device configuration of a stirring dyeing unit.

The present apparatus has five pipettes 201 for staining leukocytes, five pipettes for staining reticulocytes 202, and these direct mixing pipettors for vertically supporting the direct mixing pipettors circumferentially at equal intervals. A dyeing pipetter fixing disk 209, a rotary elevating and lowering drive mechanism 403 that repeats the operation of supporting the center of the dyeing pipetter fixing disk 209 and lowering when it rotates 36 degrees and rising again after a predetermined time has elapsed and rotating 36 degrees. Passage holes are provided on the same circumference at intervals of 36 degrees, one of which is a dye pipette fixing disk 2
And a flow passage switching valve 404 that switches the flow passages every 36 degrees with rotation of the pipette fixing disc 209 while the other is fixed to the main body.
Dyeing pipette washing tank 1 for washing dyeing pipettors
19, a white blood cell staining sample port 115, a reticulocyte staining sample port 116, a white blood cell and reticulocyte counting flow cell 301, a laser light source 304,
And a forward scatter detector 305.

Next, an example of the apparatus configuration of the stirring section using the direct mixing pipettor will be described. This device has four pipettes 203 for counting red blood cells, four pipettes for counting white blood cells 204, and a stirring pipette fixed for supporting the direct mixing pipettes vertically downward at equal intervals on the circumference thereof. And a rotary elevating drive mechanism 403 for repeating the operation of supporting the center of the disk 210 for stirring and the disk 210 for fixing the agitating pipette, descending when rotated by 45 degrees, and rising again after a lapse of a predetermined time and rotating by 45 degrees; Holes are provided on the same circumference at every 45 degrees, one is fixed to the pipette fixing disk 210 and the other is fixed to the main body, and the flow path is made every 45 degrees as the fixing disk 210 rotates. The flow path switching valve 405 for sequentially switching the flow rate, the red blood cell counting pipette cleaning tank 120, the white blood cell counting pipette cleaning tank 121, and the diluted sample sun. And Rupoto 117, white blood cell count for the sample port 11
8, a red blood cell counting flow cell 302, a white blood cell counting flow cell 303, an insulating chamber 306, and a counting signal processing device 307.

Next, the sample port will be described in detail. FIG. 6 shows an example of the configuration of the sample port (connection unit) 115. The sample port 115 is formed of, for example, chlorofluorocarbon resin, a sample liquid feeding tube 407 is connected to the lower portion, and a cleaning suction pipe 406 is provided on the upper portion. When the direct mixing pipettor 401 is inserted from above, the sample port 115 is connected to the sample port 115 while maintaining airtightness, and the sample sent to the sample port 115 is directly supplied to the direct mixing pipetter 201.
Can be sent to.

Next, an example of the structure of the fluorescence detecting section will be described. FIG. 7 shows an example of the configuration of the fluorescence detecting section. The fluorescence detection unit includes a laser light source 304 and a flow cell 30 that contracts the sample.
1, forward scatter detector 305, and side scatter detector 306
And a signal processing circuit 514 and a data processing circuit 506.

Next, the structure of the sample counting chamber is shown in FIG.
Will be explained. FIG. 8 shows an example of the structure of the sample counting chamber. The sample counting chamber is the chamber body 3
02, and sample passage pores 30 through which sample particles pass
9, a waste liquid tube 408, an electrode 308 for measuring resistance before and after the pore, an insulating chamber 306 for insulating the liquid before and after the pore, and an upper sheath liquid for flowing sample particles into the pore 309. And an upper sheath liquid supply tube 518 for supplying a lower sheath liquid that flows to the waste liquid tube 408 without returning the sample that has passed through the pores to the fine pores again, and an overflowed sheath liquid. And an overflow suction pipe 517 for sucking the air.

Next, the structure of the control device of this device will be described with reference to FIG. FIG. 9 is a configuration example of the control device. The control device has a central control circuit 501 that controls the entire device.
A keyboard 502 for an operator to input to the central control circuit, and a display 503 for displaying measurement data and the like.
A recording device 504 for printing measurement data and the like, a system control circuit 505 for controlling the drive system control circuit 507 controlled by a central control circuit, and a motor, valve, etc. driven by the drive system control circuit 507. Device 508, fluorescence detector 510, red blood cell detector 511
, White blood cell detector 512, and hemoglobin detector 513
A detector control circuit 509 that is controlled by the system control circuit 505 to control the detector; a signal processing circuit 514 that converts the output from the detector into a signal; and a signal processing circuit 51.
The data processing circuit 506 that transmits the signal from the No. 4 as data to the central control circuit 510, the operation monitoring sensor 515 that checks the operation, and the signal from the operation monitoring sensor 515 are detected and transmitted to the central control circuit 501. And a detection circuit 516.

The operation of this embodiment will be described. First, the operation of the reagent supply unit will be described. (1) The sample collecting device 102 is connected to the sample test tube 103.
Set to. (2) With the quantitative sampling valve 101 in the state shown in FIG. 1, the piston of the sample suction syringe 110 is driven downward by the motor to suck the sample in the sample test tube 103. At this time, the sample is sucked up to the sample transport tube 146, the white blood cell staining sample collecting section 123, the one-step dilution sample collecting section 124, the white blood cell counting sample collecting section 125, and a part of the sample transport tube 134.

(3) The quantitative sampling valve is rotated to the position of 147, the flow path 123 in the sampling valve,
Quantify the sample using 124, 125.

(4) The sample collecting device 102 is removed from the sample test tube 103. (5) The three-way valve 148 is opened, and the washing liquid is supplied with the sample transport tube 134 and the sample tube washing parts 141, 14
2, 145 and the sample transport tube 146 are passed through, and the sample collecting device 102 is discharged to wash these flow paths. Further, the valve 149 is opened, and the sample collecting device 1
The outside of No. 02 is washed. (6) The piston of the leukocyte staining syringe 104 is driven upward by a motor, and a fixed amount of the leukocyte staining reagent 111 is passed through the leukocyte staining reagent transport tube 128, together with the sample collected by the leukocyte staining sample collecting section 123, together with the leukocytes. It is sent to the leukocyte staining sample port 115 via the staining reagent transport tube 135.

At this time, the leukocyte staining pipette 201 is
The staining pipettor fixing disc 209 is connected to the leukocyte staining sample port 115 by moving to a predetermined position. Next, by moving the piston of the leukocyte staining syringe 205 upward and driving the hydraulic fluid 211, the sample is transported from the leukocyte staining sample port 115, and at the same time, all of the leukocyte staining pipette 201 is not leaked. Aspirate to. The hydraulic fluid and sample are separated by segmented air and do not mix. After the suction of the sample is completed, the leukocyte staining pipette 201 is removed from the leukocyte staining sample port 115 by moving the staining pipette fixing disk 209 to a predetermined position.

Thereafter, the agitation described later is performed inside the white blood cell staining pipette 201. Since the leukocyte staining pipetter 201 can stir a small amount of sample, it is possible to reduce the required sample amount and reagent amount. In addition, since the white blood cell staining sample port 115 is much smaller than the conventionally used stirring chamber, the amount of reagent for washing can be significantly reduced. (7) The piston of the leukocyte-staining syringe 104 is driven upward to feed the leukocyte-staining reagent 104 to wash the leukocyte-staining channel system (6). At the white blood cell staining sample port 115, the valve 150 is opened and the washing reagent is discharged.

(8) Similar to (6) and (7), after moving the staining pipettor fixing disk 209 to a predetermined position,
The white blood cell counting syringe 109 is driven to supply a fixed amount of white blood cell counting reagent 114 to the white blood cell counting sample port 118.
The liquid is sucked into the white blood cell counting pipette 204 via the. (9) The first-stage dilution syringe 108 is driven, a fixed amount of the diluent 113 is passed through the first-stage diluent transport tube 132, and the first-stage diluent transport tube 138 together with the sample collected by the leukocyte-staining sample collector 123. Then, the solution is sent to the stirring chamber 122 for one-step dilution. The stirring chamber 122 for first-stage dilution stirs the sample and the diluent 113. (10) The quantitative sampling valve 101 is rotated to return to the state shown in FIG. (11) The three-way valve 148 is opened, and the flow path of the sampling valve through which the next sample is sucked is washed.

(12) The piston of the suction syringe for the 1st-step dilution liquid is driven downward to bring the 1st-step dilution liquid that has been agitated in (9) downward, and the 1st-step dilution solution feed tube 140 and the 2-step dilution sample are collected. The solution is sent to the section 126 and the reticulocyte red blood cell staining sample collecting section 127. (13) Rotate the quantitative sampling valve to the position of 147. (14) Similar to (6) and (7), after moving the stirring pipettor fixing disk 210 to a predetermined position, the reticulocyte staining syringe 105 is driven and the fixed amount of reticulocyte staining reagent 112 is reticulated. Sample port 11 for red blood cell staining
Pipette 20 with chamber for reticulocyte staining via 6
Aspirate to 2.

(15) Similar to (6) and (7), after moving the stirring pipettor fixing disk 210 to a predetermined position, the two-step dilution syringe 107 is driven and the fixed amount of the diluted liquid 1
13 through the sample port 117 for diluted sample,
Aspirate to the pipettor 203 with the chamber for counting red blood cells. (16) The three-way valve 151 is opened and the washing liquid is transferred to the first-stage diluting liquid transport tube 130 and the sample tube washing section 14.
4,143, through the 1-stage dilution liquid transport tube 140,
It is sent to the stir chamber 122 for serial dilution to wash them. The waste liquid is discharged by opening the valve 152.

Next, the operation of the stirring dyeing section will be described (the operations of (1) and (2) are also duplicated in (6) of the operation of the reagent supply section). (1) The direct mixing pipetter 201 is connected to the sample port 115 by moving the staining pipettor fixing disk 209 to a predetermined position. (2) With the operation of the reagent supply unit, the piston of the leukocyte staining syringe 205 is driven upward at the same time when the reagent and the sample are fed. As a result, the sample and the reagent are sucked into the pipetter 201 without leaking from the sample port 115. After that, the staining pipettor fixing disc 209 is moved to a predetermined position, so that the direct mixing pipettor 2 is moved from the sample port 115.
Release 01. (3) The reagent and the sample are stirred and stained in the staining chamber of the direct mixing pipetter 201. By repeatedly driving the piston of the white blood cell staining syringe 205 up and down with a motor, the hydraulic fluid 211 is driven, and a swirl flow is generated in the sample in the chamber at the rapid expansion and contraction portions of the flow path. The sample and reagent are fluidly agitated.

In this method, since it is possible to stir in a minute chamber, it is possible to stir a minute amount of sample and reagent. Further, since all the samples and reagents are taken into the chamber from the sample reagent supply unit, the time for which the sample stays in the sample reagent supply unit can be shortened, and high-speed processing can be performed.

(4) After the stirring dyeing operation is completed, the direct mixing pipetter 201 is moved to the predetermined position by moving the dyeing pipettor fixing disk 209 to a predetermined position.
It is connected to the white blood cell measurement flow cell 301. (5) The sample is ejected from the direct mixing pipetter 201 by driving the white blood cell staining syringe 205 downward. (6) The direct mixing pipetter 201 is set in the staining pipettor washing tank 119 by moving the staining pipettor fixing disc 209 to a predetermined position, and the washing liquid is caused to flow through the leukocyte staining syringe 205 to remove the pipette 201. Perform cleaning. (7) The pipette 20 is operated in the same manner as (1) to (6).
After stirring or dyeing each of No. 2, 203, and 204, each measurement and washing are performed.

Next, the operation of the particle measuring section will be described. (1) In a flow cell (sheath flow cell) 301 for measuring white blood cells and reticulocytes, a sheath flow is used to contract the sample, and the sample particles flowing in the thin tube portion are
Align one by one. Laser light is irradiated to each sample particle from the laser light source 304 to the converging portion, whereby the particles generate fluorescence or scattered light, which are detected by the fluorescence detection device 305. (2) In the erythrocyte count flow cell 302, the sample is contracted by a sheath flow (enclosing flow), and the sample particles pass through the pores 309 one by one.
At this time, the variation in resistance generated in the electrode 308 is measured, and the number of red blood cells in the sample per unit volume is measured by the electric resistance method. Insulation chamber 306 between sheath liquid and waste liquid
Is insulated with. (3) The white blood cell count flow cell 303 measures the white blood cell count in the same manner as (2).

Next, the timing of the stirring dyeing operation will be described with reference to FIG. FIG. 10 is a time chart of the stirring dyeing operation operation. (1) As shown in FIG.
The same operation is repeated every 0 seconds. (2) Among the stirring and staining operations, the operations of the white blood cell staining chamber and the reticulocyte staining-like chamber are as shown in FIG.
The same operation is repeated in a cycle of 00 seconds. The staining chamber is
As shown in FIG. 5, ten specimens are arranged on the circumference, and the specimens are treated in a cycle of 20 seconds by rotating this and performing treatment in each chamber. (3) Among the agitation staining operations, the operations of the red blood cell counting chamber and the white blood cell counting chamber repeat similar operations at a cycle of 80 seconds as shown in FIG. The stirring chambers are arranged on the circumference of eight circles as shown in FIG. 3, and the stirring chambers are rotated to perform the processing in each chamber, thereby processing the sample in a cycle of 20 seconds.

(4) As for the operation of the measuring section, as shown in FIG. 10, the same operation is repeated every 20 seconds. The operations (1) to (4) can be performed in an overlapping manner.
That is, one sample is processed in 20 seconds at the timing shown in FIG. Therefore, in the case of continuously inspecting the sample with this device, it is possible to perform the inspection of four items for up to 180 samples in one hour. This is because the processing is performed using a plurality of pipettes with chambers. That is, the transportation of the sample and reagent in the tube, which hinders high throughput, is minimized.

[0049]

According to the present invention, many types of test items such as red blood cell count, white blood cell count, white blood cell classification, and reticulocyte count of a particle suspension can be measured with high throughput, and necessary at that time. It is possible to provide a biological particle measuring device in which a small amount of various samples and reagents are used.

[Brief description of drawings]

FIG. 1 is a configuration diagram showing an embodiment of the present invention.

FIG. 2 is an enlarged view of a reagent supply unit and a particle measurement unit shown in FIG.

FIG. 3 is an enlarged view of a stirring and dyeing section shown in FIG.

FIG. 4 is an enlarged cross-sectional view of the pipettor shown in FIG.

5 is a perspective view of the stirring dyeing unit shown in FIG. 1. FIG.

FIG. 6 is an enlarged cross-sectional view of a connection portion shown in FIG.

FIG. 7 is a configuration diagram of a fluorescence detection unit shown in FIG.

8 is a block diagram of the sample counting chamber shown in FIG. 1. FIG.

FIG. 9 is a block diagram showing a configuration of a control device.

FIG. 10 is a diagram showing a time chart of the invention.

[Explanation of symbols]

100 Reagent Supply Unit 200 Stirred Staining Unit 300 Measuring Unit 101 Quantitative Sampling Valve 102 Sample Collection Device 103 Whole Blood Sample 104 Leukocyte Staining Reagent Syringe 105 Reticulocyte Staining Reagent Syringe 106 1-Level Diluting Syringe 107 107 2-Level Diluting Syringe 108 1-step dilution syringe 109 Leukocyte counting reagent syringe 110 Sample suction syringe 111 Leukocyte staining reagent 112 Reticulocyte staining reagent 113 Diluting solution 114 Leukocyte counting reagent 115 Leukocyte staining sample port (connection) 116 Reticulocyte staining sample Port 117 Sample port for diluted sample 118 White blood cell counting sample port 119 Staining pipette washing tank 120 Red blood cell counting pipette washing tank 121 White blood cell counting pipette washing tank 122 1 stage Stirring chamber for dilution 123 Leukocyte staining sample collection section 124 1-step dilution sample collection section 125 Leukocyte counting sample collection section 126 Two-step dilution sample collection section 127 Reticulocyte staining sample collection section 128 Leukocyte staining reagent transport tube 129 Reticulocyte staining reagent transport tube 130 1-step dilution solution transport tube 131 2-step dilution solution transport tube 132 1-step dilution solution transport tube 133 Leukocyte counting reagent transport tube 134 Sample transport tube 135 Leukocyte staining reagent transport tube 136 Reticulocyte staining Reagent transport tube 137 Two-step dilution solution transport tube 138 One-step dilution solution transport tube 139 Leukocyte counting reagent transport tube 140 One-step dilution solution transport tube 141, 142, 143, 144, 145 Sample tube washing Part 146 Sample transport tube 147 Sampling valve rotation position 148 to 152 Electromagnetic valve 201 Leukocyte staining pipette 202 Reticulocyte staining pipette 203 Red blood cell counting pipette 204 Leukocyte counting pipette 205 Leukocyte staining syringe 206 Reticulocyte staining syringe 207 Red blood cell counting Syringe 208 Leukocyte counting syringe 209 Staining pipette fixing disk 210 Stirring pipette fixing disk 211 Working fluid 301 Flow cell for measuring leukocytes and reticulocytes 302 Flow cell for counting red blood cell 303 Flow cell for counting white blood cell 304 Laser light source 305 Fluorescence detection device 306 Insulation chamber 307 Count signal processing device 308 Electrode 309 Sample passing pore (pore) 310 Compressor 311 Cleaning liquid bottle 312 Waste liquid Draw bottle 401 Pipette pipe 402 Micro chamber (reaction tank) 403 Rotation lifting drive mechanism 404 Flow path switching valve 405 Flow path switching valve 406 Suction tube for washing 407 Sample liquid feeding tube 408 Waste liquid tube 409 Upper sheath liquid supply tube 410 Lower sheath liquid supply Tube 501 Central control circuit 502 Keyboard 503 Display device 504 Recording device 505 System control circuit 506 Data processing circuit 507 Drive system control circuit 508 Drive device 509 Detector control circuit 510 Fluorescence detector 511 Red blood cell detector 512 White blood cell detector 513 Reticulocyte detection 514 Signal processing circuit 515 Operation monitoring sensor 516 Abnormality detection circuit

 ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Inventor Isao Yamazaki 502 Jinritsu-cho, Tsuchiura-shi, Ibaraki Machinery Research Institute, Hiritsu Manufacturing Co., Ltd. (72) Hideyuki Horiuchi 882, Ige, Katsuta-shi, Ibaraki Hitachi Ltd. Naka Inside the factory (72) Inventor Ryohei Yanabe 882, Ige, Katsuta-shi, Ibaraki Hitachi Ltd. Naka factory

Claims (10)

[Claims] 1. A biological particle measuring device having a particle measuring unit, and a quantitative sampling device for quantitatively sampling a particle suspension, and at least one transportation means for transporting the quantitatively sampled particle suspension. At least one connecting portion for connecting the transported particle suspension, and at least one pipettor for sucking the particle suspension from each connecting portion to stir the particle suspension, respectively. And a particle measuring unit having at least one flow cell that contracts the particle suspension discharged from the pipettor. 2. A biological particle measuring device equipped with a particle measuring unit, wherein a quantitative sampling device for quantitatively sampling a particle suspension and a reagent for dilution and reaction are supplied to the quantitatively sampled particle suspension. And at least one reagent supplying means for transporting, at least one connecting part for connecting the transported particle suspension, and suctioning the particle suspension from each connecting part A biological particle measuring apparatus comprising: at least one pipettor for stirring and at least one particle measuring unit having at least one flow cell for contracting the particle suspension discharged from each pipettor. 3. At least one flow cell is a sheath flow cell having a constriction part for constricting a particle suspension and a thin tube part in which particles of the particle suspension are aligned and flow. The biological particle measuring device according to claim 1 or 2. 4. At least one flow cell has a constriction part for constricting a particle suspension and pores in which the particles of the particle suspension are aligned and flow, and a potential difference between front and rear is provided. The biological particle measuring device according to claim 1 or 2, which is an electric resistance type cell for measuring the properties of the particles by allowing the particles to pass through the pores. 5. The quantitative sampling device comprises a quantitative sampling valve for performing quantitative measurement by switching a part of the first flow path filled with the collected particle suspension to a part of the second flow path. The biological particle measuring apparatus according to claim 1 or 2, further comprising: 6. The biological particle measuring device according to claim 1, wherein each pipettor is provided with a reaction tank in which the particle suspension is mixed with a reagent and reacted. 7. The biological particle measuring device according to claim 1, further comprising a plurality of said quantitative sampling devices and a plurality of said particle measuring parts, wherein a plurality of particle suspensions are simultaneously measured. 8. A plurality of said quantitative sampling devices and said particle measuring units are provided, and at least one distribution / supply means for distributing and supplying the same particle suspension to each particle measuring unit,
The biological particle measuring device according to claim 1 or 2, wherein the properties of a plurality of types of particle suspensions are measured at the same time. 9. A biological particle measuring apparatus having a particle measuring unit, wherein a particle suspension is sucked into a first flow path thereof, and a plurality of parts of the first flow path are one of a plurality of other flow paths. A plurality of quantitative sampling valves for performing a large number of quantitative determinations at once by switching to different parts, and a plurality of reagents for supplying a plurality of reagents to each of the plurality of flow paths and for transporting together with the quantitatively sampled particle suspension. A supply means, a plurality of connecting portions for connecting the transported particle suspension, and a plurality of pipettors for agitating the particle suspension by sucking the particle suspension from the respective connecting portions, A biological particle measuring device, comprising: a plurality of particle measuring units having a plurality of flow cells that contract the particle suspension discharged from each pipettor. 10. A two-step reaction means for transporting a once-quantified particle suspension together with a reagent to a predetermined container for mixing and reacting, and then sucking again from the container to a quantitative sampling valve to perform another quantitative determination. The biological particle measuring device according to claim 9, wherein