JPH05501052A - 核酸ハイブリッド形成アッセイを使用する細菌の定量 - Google Patents
核酸ハイブリッド形成アッセイを使用する細菌の定量Info
- Publication number
- JPH05501052A JPH05501052A JP2510746A JP51074690A JPH05501052A JP H05501052 A JPH05501052 A JP H05501052A JP 2510746 A JP2510746 A JP 2510746A JP 51074690 A JP51074690 A JP 51074690A JP H05501052 A JPH05501052 A JP H05501052A
- Authority
- JP
- Japan
- Prior art keywords
- probe
- bacteria
- sample
- nucleic acid
- hybridization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
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- PZYDAVFRVJXFHS-UHFFFAOYSA-N n-cyclohexyl-2-pyrrolidone Chemical compound O=C1CCCN1C1CCCCC1 PZYDAVFRVJXFHS-UHFFFAOYSA-N 0.000 description 1
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- 230000007935 neutral effect Effects 0.000 description 1
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- 150000007524 organic acids Chemical class 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
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- 201000001245 periodontitis Diseases 0.000 description 1
- 125000000864 peroxy group Chemical group O(O*)* 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-M phenolate Chemical compound [O-]C1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-M 0.000 description 1
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- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Small-Scale Networks (AREA)
- Communication Control (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US37835589A | 1989-07-11 | 1989-07-11 | |
| US378,355 | 1989-07-11 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH05501052A true JPH05501052A (ja) | 1993-03-04 |
Family
ID=23492807
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2510746A Pending JPH05501052A (ja) | 1989-07-11 | 1990-07-10 | 核酸ハイブリッド形成アッセイを使用する細菌の定量 |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP0484385A4 (fr) |
| JP (1) | JPH05501052A (fr) |
| AU (1) | AU6075490A (fr) |
| WO (1) | WO1991000926A1 (fr) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08297A (ja) * | 1994-06-17 | 1996-01-09 | Becton Dickinson & Co | バクテリアを検出するためのオリゴヌクレオチドプライマーおよびプローブ |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6060237A (en) | 1985-02-26 | 2000-05-09 | Biostar, Inc. | Devices and methods for optical detection of nucleic acid hybridization |
| EP0502271A1 (fr) * | 1989-04-17 | 1992-09-09 | The Standard Oil Company | Sondes d'oligonucléotides de 16s rDNA pour l'identification de bactéries sulfato-réductrices |
| US5656427A (en) * | 1994-08-29 | 1997-08-12 | Gen-Probe Incorporated | Nucleic acid hybridization assay probes, helper probes and amplification oligonucleotides targeted to Mycoplasma pneumoniae nucleic acid |
| FR2733754B1 (fr) * | 1995-05-05 | 1997-05-30 | Univ Angers | Fragment de l'adn genomique de clavibacter michiganensis, sonde d'hybridation, amorce d'amplification, reactif et procede de detection de clavibacter michiganensis |
| US5925518A (en) * | 1995-05-19 | 1999-07-20 | Akzo Nobel N.V. | Nucleic acid primers for amplification of a mycobacteria RNA template |
| DE19709881A1 (de) * | 1997-03-11 | 1998-09-17 | Huels Chemische Werke Ag | Verfahren zur Quantifizierung von Bakterien |
| FR2769323B1 (fr) | 1997-10-08 | 2001-07-13 | Suez Lyonnaise Des Eaux | Moyens pour l'analyse qualitative et quantitative des populations microbiennes eventuellement presentes dans un echantillon |
| JP2002509731A (ja) * | 1998-03-27 | 2002-04-02 | セイジーン・コーポレーション | 混合培養中の生きた微生物の存在を決定する選択的アッセイ法 |
| US7465540B2 (en) | 2000-09-21 | 2008-12-16 | Luminex Corporation | Multiple reporter read-out for bioassays |
| DE10215238C1 (de) * | 2002-04-06 | 2003-08-14 | Cytonet Gmbh & Co Kg | Nachweis von Mykobakterien in klinischem Material |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB8311905D0 (en) * | 1983-04-29 | 1983-06-02 | Cox R A | Isolation and detection of nucleotide sequence |
| FI72146C (fi) * | 1985-01-02 | 1987-04-13 | Orion Yhtymae Oy | Foerfarande foer identifiering av nukleinsyror. |
| CA1301606C (fr) * | 1986-05-02 | 1992-05-26 | David H. Gillespie | Methode chaotropique pour l'evaluation d'acides nucleiques dans un echantillon biologique |
| WO1988003957A1 (fr) * | 1986-11-24 | 1988-06-02 | Gen-Probe Incorporated | Sondes d'acide nucleique pour la detection et/ou la quantification d'organismes non viraux |
| FI903485A7 (fi) * | 1988-01-11 | 1990-07-10 | Microprobe Corp | Oligonukleotidikoettimia periodontaalisten patogeenien toteamiseen |
-
1990
- 1990-07-10 AU AU60754/90A patent/AU6075490A/en not_active Abandoned
- 1990-07-10 WO PCT/US1990/003880 patent/WO1991000926A1/fr not_active Ceased
- 1990-07-10 JP JP2510746A patent/JPH05501052A/ja active Pending
- 1990-07-10 EP EP19900911234 patent/EP0484385A4/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08297A (ja) * | 1994-06-17 | 1996-01-09 | Becton Dickinson & Co | バクテリアを検出するためのオリゴヌクレオチドプライマーおよびプローブ |
Also Published As
| Publication number | Publication date |
|---|---|
| EP0484385A4 (en) | 1993-05-26 |
| AU6075490A (en) | 1991-02-06 |
| EP0484385A1 (fr) | 1992-05-13 |
| WO1991000926A1 (fr) | 1991-01-24 |
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