JPH0550268B2 - - Google Patents

Info

Publication number: JPH0550268B2
Authority: JP; Japan
Prior art keywords: acid; culture; lipase; pseudomonas; growth
Prior art date: 1986-03-12
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

JP5450886A

Other languages

English (en)

Japanese (ja)

Other versions

JPS62210981A (ja

Inventor

Shigeo Inoe

Yoshiharu Kimura

Akira Takei

Kazuaki Fushimi

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

SEITAI KINO RYO KAGAKUHIN

SEITAI KINO RYO KAGAKUHIN SHINSEIZO GIJUTSU KENKYU KUMIAI

Original Assignee

SEITAI KINO RYO KAGAKUHIN

SEITAI KINO RYO KAGAKUHIN SHINSEIZO GIJUTSU KENKYU KUMIAI

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1986-03-12

Filing date

1986-03-12

Publication date

1993-07-28

1986-03-12 Application filed by SEITAI KINO RYO KAGAKUHIN, SEITAI KINO RYO KAGAKUHIN SHINSEIZO GIJUTSU KENKYU KUMIAI filed Critical SEITAI KINO RYO KAGAKUHIN

1986-03-12 Priority to JP5450886A priority Critical patent/JPS62210981A/ja

1987-09-17 Publication of JPS62210981A publication Critical patent/JPS62210981A/ja

1993-07-28 Publication of JPH0550268B2 publication Critical patent/JPH0550268B2/ja

Status Granted legal-status Critical Current

Links

229920001817 Agar Polymers 0.000 claims description 12
239000008272 agar Substances 0.000 claims description 12
230000007062 hydrolysis Effects 0.000 claims description 12
238000006460 hydrolysis reaction Methods 0.000 claims description 12
238000006243 chemical reaction Methods 0.000 claims description 11
IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 10
241000589774 Pseudomonas sp. Species 0.000 claims description 9
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 8
241000589516 Pseudomonas Species 0.000 claims description 7
235000000346 sugar Nutrition 0.000 claims description 7
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 6
-1 L-rhamnose - levulinic acid - D-tartaric acid Chemical compound 0.000 claims description 6
230000015572 biosynthetic process Effects 0.000 claims description 6
KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 6
238000004519 manufacturing process Methods 0.000 claims description 6
239000012528 membrane Substances 0.000 claims description 5
229910052757 nitrogen Inorganic materials 0.000 claims description 5
150000008163 sugars Chemical class 0.000 claims description 5
WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 claims description 4
SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 claims description 4
229920002472 Starch Polymers 0.000 claims description 4
239000002253 acid Substances 0.000 claims description 4
PYMYPHUHKUWMLA-LMVFSUKVSA-N aldehydo-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 claims description 4
SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 4
239000007789 gas Substances 0.000 claims description 4
239000008107 starch Substances 0.000 claims description 4
235000019698 starch Nutrition 0.000 claims description 4
KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims description 3
235000011187 glycerol Nutrition 0.000 claims description 3
238000009630 liquid culture Methods 0.000 claims description 3
235000013372 meat Nutrition 0.000 claims description 3
HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims description 3
235000013336 milk Nutrition 0.000 claims description 3
239000008267 milk Substances 0.000 claims description 3
210000004080 milk Anatomy 0.000 claims description 3
230000001766 physiological effect Effects 0.000 claims description 3
239000000049 pigment Substances 0.000 claims description 3
HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 2
CDVZCUKHEYPEQS-SNQKNWKTSA-N (2r,3s,4r)-2,3,4,5-tetrahydroxypentanal;(2r,3s,4s)-2,3,4,5-tetrahydroxypentanal Chemical compound OC[C@H](O)[C@H](O)[C@@H](O)C=O.OC[C@@H](O)[C@H](O)[C@@H](O)C=O CDVZCUKHEYPEQS-SNQKNWKTSA-N 0.000 claims description 2
OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 claims description 2
239000004475 Arginine Substances 0.000 claims description 2
102000016938 Catalase Human genes 0.000 claims description 2
108010053835 Catalase Proteins 0.000 claims description 2
RFSUNEUAIZKAJO-VRPWFDPXSA-N D-Fructose Natural products OC[C@H]1OC(O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-VRPWFDPXSA-N 0.000 claims description 2
UNXHWFMMPAWVPI-QWWZWVQMSA-N D-Threitol Natural products OC[C@@H](O)[C@H](O)CO UNXHWFMMPAWVPI-QWWZWVQMSA-N 0.000 claims description 2
FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 claims description 2
WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 claims description 2
SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims description 2
RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 claims description 2
UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 claims description 2
RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 claims description 2
108010010803 Gelatin Proteins 0.000 claims description 2
238000003794 Gram staining Methods 0.000 claims description 2
KZSNJWFQEVHDMF-BYPYZUCNSA-N L-Valine Natural products CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 claims description 2
GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 2
GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 claims description 2
229910002651 NO3 Inorganic materials 0.000 claims description 2
NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims description 2
102000004316 Oxidoreductases Human genes 0.000 claims description 2
108090000854 Oxidoreductases Proteins 0.000 claims description 2
MUPFEKGTMRGPLJ-RMMQSMQOSA-N Raffinose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 MUPFEKGTMRGPLJ-RMMQSMQOSA-N 0.000 claims description 2
NGFMICBWJRZIBI-JZRPKSSGSA-N Salicin Natural products O([C@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@H](CO)O1)c1c(CO)cccc1 NGFMICBWJRZIBI-JZRPKSSGSA-N 0.000 claims description 2
229930006000 Sucrose Natural products 0.000 claims description 2
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 2
FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims description 2
HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 2
MUPFEKGTMRGPLJ-UHFFFAOYSA-N UNPD196149 Natural products OC1C(O)C(CO)OC1(CO)OC1C(O)C(O)C(O)C(COC2C(C(O)C(O)C(CO)O2)O)O1 MUPFEKGTMRGPLJ-UHFFFAOYSA-N 0.000 claims description 2
108010046334 Urease Proteins 0.000 claims description 2
HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 2
NGFMICBWJRZIBI-UHFFFAOYSA-N alpha-salicin Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=CC=C1CO NGFMICBWJRZIBI-UHFFFAOYSA-N 0.000 claims description 2
PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 2
ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 2
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 2
229960003237 betaine Drugs 0.000 claims description 2
230000025938 carbohydrate utilization Effects 0.000 claims description 2
UNXHWFMMPAWVPI-ZXZARUISSA-N erythritol Chemical compound OC[C@H](O)[C@H](O)CO UNXHWFMMPAWVPI-ZXZARUISSA-N 0.000 claims description 2
235000011389 fruit/vegetable juice Nutrition 0.000 claims description 2
239000008273 gelatin Substances 0.000 claims description 2
229920000159 gelatin Polymers 0.000 claims description 2
235000019322 gelatine Nutrition 0.000 claims description 2
235000011852 gelatine desserts Nutrition 0.000 claims description 2
210000004209 hair Anatomy 0.000 claims description 2
229910000037 hydrogen sulfide Inorganic materials 0.000 claims description 2
PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 claims description 2
RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 claims description 2
239000008101 lactose Substances 0.000 claims description 2
230000001590 oxidative effect Effects 0.000 claims description 2
239000001301 oxygen Substances 0.000 claims description 2
229910052760 oxygen Inorganic materials 0.000 claims description 2
230000009467 reduction Effects 0.000 claims description 2
229940120668 salicin Drugs 0.000 claims description 2
239000005720 sucrose Substances 0.000 claims description 2
229960004295 valine Drugs 0.000 claims description 2
238000009631 Broth culture Methods 0.000 claims 1
KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 claims 1
102000004882 Lipase Human genes 0.000 description 30
108090001060 Lipase Proteins 0.000 description 30
239000004367 Lipase Substances 0.000 description 30
235000019421 lipase Nutrition 0.000 description 30
239000003921 oil Substances 0.000 description 20
230000000694 effects Effects 0.000 description 17
102000004190 Enzymes Human genes 0.000 description 16
108090000790 Enzymes Proteins 0.000 description 16
239000002609 medium Substances 0.000 description 15
235000019198 oils Nutrition 0.000 description 15
239000000243 solution Substances 0.000 description 13
235000019482 Palm oil Nutrition 0.000 description 11
239000002540 palm oil Substances 0.000 description 11
235000015278 beef Nutrition 0.000 description 10
239000003760 tallow Substances 0.000 description 10
238000000034 method Methods 0.000 description 9
UYXTWWCETRIEDR-UHFFFAOYSA-N Tributyrin Chemical compound CCCC(=O)OCC(OC(=O)CCC)COC(=O)CCC UYXTWWCETRIEDR-UHFFFAOYSA-N 0.000 description 8
BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 8
238000000354 decomposition reaction Methods 0.000 description 7
239000003925 fat Substances 0.000 description 7
235000019197 fats Nutrition 0.000 description 7
230000003301 hydrolyzing effect Effects 0.000 description 7
239000004006 olive oil Substances 0.000 description 7
235000008390 olive oil Nutrition 0.000 description 7
CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
241000894006 Bacteria Species 0.000 description 6
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
150000003839 salts Chemical class 0.000 description 6
239000003240 coconut oil Substances 0.000 description 5
235000019864 coconut oil Nutrition 0.000 description 5
238000012258 culturing Methods 0.000 description 5
235000014113 dietary fatty acids Nutrition 0.000 description 5
239000000194 fatty acid Substances 0.000 description 5
229930195729 fatty acid Natural products 0.000 description 5
239000007788 liquid Substances 0.000 description 5
244000005700 microbiome Species 0.000 description 5
239000000203 mixture Substances 0.000 description 5
235000014593 oils and fats Nutrition 0.000 description 5
239000000843 powder Substances 0.000 description 5
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 4
230000009471 action Effects 0.000 description 4
230000001580 bacterial effect Effects 0.000 description 4
150000004665 fatty acids Chemical class 0.000 description 4
239000008103 glucose Substances 0.000 description 4
229910052697 platinum Inorganic materials 0.000 description 4
WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 description 3
LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 description 3
QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 description 3
UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 3
239000005642 Oleic acid Substances 0.000 description 3
ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 description 3
239000001888 Peptone Substances 0.000 description 3
108010080698 Peptones Proteins 0.000 description 3
KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
239000001110 calcium chloride Substances 0.000 description 3
229910001628 calcium chloride Inorganic materials 0.000 description 3
239000012228 culture supernatant Substances 0.000 description 3
238000005516 engineering process Methods 0.000 description 3
238000010438 heat treatment Methods 0.000 description 3
QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 description 3
238000002844 melting Methods 0.000 description 3
229910052751 metal Inorganic materials 0.000 description 3
239000002184 metal Substances 0.000 description 3
ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 3
235000019319 peptone Nutrition 0.000 description 3
239000002689 soil Substances 0.000 description 3
239000006228 supernatant Substances 0.000 description 3
241000589513 Burkholderia cepacia Species 0.000 description 2
OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
229910052921 ammonium sulfate Inorganic materials 0.000 description 2
235000011130 ammonium sulphate Nutrition 0.000 description 2
229940041514 candida albicans extract Drugs 0.000 description 2
239000004202 carbamide Substances 0.000 description 2
229910052799 carbon Inorganic materials 0.000 description 2
238000005119 centrifugation Methods 0.000 description 2
230000009849 deactivation Effects 0.000 description 2
238000010586 diagram Methods 0.000 description 2
230000008014 freezing Effects 0.000 description 2
238000007710 freezing Methods 0.000 description 2
238000002523 gelfiltration Methods 0.000 description 2
230000002779 inactivation Effects 0.000 description 2
235000019626 lipase activity Nutrition 0.000 description 2
230000008018 melting Effects 0.000 description 2
OIPPWFOQEKKFEE-UHFFFAOYSA-N orcinol Chemical compound CC1=CC(O)=CC(O)=C1 OIPPWFOQEKKFEE-UHFFFAOYSA-N 0.000 description 2
239000002504 physiological saline solution Substances 0.000 description 2
235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
229920000053 polysorbate 80 Polymers 0.000 description 2
LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
238000002360 preparation method Methods 0.000 description 2
230000008569 process Effects 0.000 description 2
238000000926 separation method Methods 0.000 description 2
VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
239000000126 substance Substances 0.000 description 2
238000000108 ultra-filtration Methods 0.000 description 2
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
239000012138 yeast extract Substances 0.000 description 2
IJFXRHURBJZNAO-UHFFFAOYSA-N 3-hydroxybenzoic acid Chemical compound OC(=O)C1=CC=CC(O)=C1 IJFXRHURBJZNAO-UHFFFAOYSA-N 0.000 description 1
GUBGYTABKSRVRQ-WFVLMXAXSA-N DEAE-cellulose Chemical compound OC1C(O)C(O)C(CO)O[C@H]1O[C@@H]1C(CO)OC(O)C(O)C1O GUBGYTABKSRVRQ-WFVLMXAXSA-N 0.000 description 1
241000222175 Diutina rugosa Species 0.000 description 1
241000589540 Pseudomonas fluorescens Species 0.000 description 1
240000004808 Saccharomyces cerevisiae Species 0.000 description 1
229920005654 Sephadex Polymers 0.000 description 1
239000012507 Sephadex™ Substances 0.000 description 1
230000004913 activation Effects 0.000 description 1
230000001476 alcoholic effect Effects 0.000 description 1
238000012870 ammonium sulfate precipitation Methods 0.000 description 1
229940053200 antiepileptics fatty acid derivative Drugs 0.000 description 1
239000007864 aqueous solution Substances 0.000 description 1
239000003613 bile acid Substances 0.000 description 1
150000001720 carbohydrates Chemical class 0.000 description 1
235000014633 carbohydrates Nutrition 0.000 description 1
230000015556 catabolic process Effects 0.000 description 1
230000008859 change Effects 0.000 description 1
RCTYPNKXASFOBE-UHFFFAOYSA-M chloromercury Chemical compound [Hg]Cl RCTYPNKXASFOBE-UHFFFAOYSA-M 0.000 description 1
238000005345 coagulation Methods 0.000 description 1
230000015271 coagulation Effects 0.000 description 1
238000006731 degradation reaction Methods 0.000 description 1
ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
229910000396 dipotassium phosphate Inorganic materials 0.000 description 1
235000019797 dipotassium phosphate Nutrition 0.000 description 1
150000002148 esters Chemical class 0.000 description 1
238000012869 ethanol precipitation Methods 0.000 description 1
239000000284 extract Substances 0.000 description 1
238000000855 fermentation Methods 0.000 description 1
230000004151 fermentation Effects 0.000 description 1
238000001914 filtration Methods 0.000 description 1
125000005456 glyceride group Chemical group 0.000 description 1
239000001963 growth medium Substances 0.000 description 1
229910052739 hydrogen Inorganic materials 0.000 description 1
230000005764 inhibitory process Effects 0.000 description 1
150000002500 ions Chemical class 0.000 description 1
238000002955 isolation Methods 0.000 description 1
229910052943 magnesium sulfate Inorganic materials 0.000 description 1
235000019341 magnesium sulphate Nutrition 0.000 description 1
238000000691 measurement method Methods 0.000 description 1
239000013028 medium composition Substances 0.000 description 1
230000000813 microbial effect Effects 0.000 description 1
229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
235000019796 monopotassium phosphate Nutrition 0.000 description 1
238000006386 neutralization reaction Methods 0.000 description 1
125000001477 organic nitrogen group Chemical group 0.000 description 1
239000003960 organic solvent Substances 0.000 description 1
239000008363 phosphate buffer Substances 0.000 description 1
GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
238000001742 protein purification Methods 0.000 description 1
238000000746 purification Methods 0.000 description 1
239000002994 raw material Substances 0.000 description 1
238000011084 recovery Methods 0.000 description 1
HEBKCHPVOIAQTA-ZXFHETKHSA-N ribitol Chemical compound OC[C@H](O)[C@H](O)[C@H](O)CO HEBKCHPVOIAQTA-ZXFHETKHSA-N 0.000 description 1
238000005185 salting out Methods 0.000 description 1
235000021003 saturated fats Nutrition 0.000 description 1
150000004671 saturated fatty acids Chemical class 0.000 description 1
235000003441 saturated fatty acids Nutrition 0.000 description 1
239000000344 soap Substances 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
239000004317 sodium nitrate Substances 0.000 description 1
235000010344 sodium nitrate Nutrition 0.000 description 1
230000006641 stabilisation Effects 0.000 description 1
238000011105 stabilization Methods 0.000 description 1
230000001954 sterilising effect Effects 0.000 description 1
238000004659 sterilization and disinfection Methods 0.000 description 1
238000003756 stirring Methods 0.000 description 1
238000003860 storage Methods 0.000 description 1
239000000758 substrate Substances 0.000 description 1
239000000725 suspension Substances 0.000 description 1
238000010257 thawing Methods 0.000 description 1
238000004448 titration Methods 0.000 description 1

Landscapes

Enzymes And Modification Thereof (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

JP5450886A 1986-03-12 1986-03-12 新規微生物 Granted JPS62210981A (ja)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
JP5450886A JPS62210981A (ja)	1986-03-12	1986-03-12	新規微生物

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP5450886A JPS62210981A (ja)	1986-03-12	1986-03-12	新規微生物

Publications (2)

Publication Number	Publication Date
JPS62210981A JPS62210981A (ja)	1987-09-17
JPH0550268B2 true JPH0550268B2 (cs)	1993-07-28

Family

ID=12972579

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP5450886A Granted JPS62210981A (ja)	1986-03-12	1986-03-12	新規微生物

Country Status (1)

Country	Link
JP (1)	JPS62210981A (cs)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP2001017989A (ja) *	1999-07-07	2001-01-23	Ebara Corp	油脂含有排水あるいは油脂含有汚泥の嫌気性処理方法

1986
- 1986-03-12 JP JP5450886A patent/JPS62210981A/ja active Granted

Also Published As

Publication number	Publication date
JPS62210981A (ja)	1987-09-17

Publication	Publication Date	Title
JPH04278087A (ja)	1992-10-02	新規ヘパリチナーゼ、その製造法及びその生産菌
CA1285896C (en)	1991-07-09	.alpha.-1, 6-GLUCOSIDASE AND PROCESS FOR PRODUCING THE SAME
JPH0755147B2 (ja)	1995-06-14	新規リパ−ゼ
JPH0550268B2 (cs)	1993-07-28
JPH0755148B2 (ja)	1995-06-14	リパ−ゼの製造法
JP3514799B2 (ja)	2004-03-31	リパーゼ及びこれを産生する微生物
JP3026312B2 (ja)	2000-03-27	キチン分解物の製造法
JP3516104B2 (ja)	2004-04-05	ムタナーゼ産生微生物及びムタナーゼ
JPH0515387A (ja)	1993-01-26	細菌によるアルギン酸の分解法
KR960007741B1 (ko)	1996-06-11	신규 α-1, 6-글루코시다제 및 그의 제조방법
JP2623507B2 (ja)	1997-06-25	マルトオリゴ糖の製造法
JPH088859B2 (ja)	1996-01-31	シェードモナスｋｗｉ−５６菌株
JPH0339086A (ja)	1991-02-20	シュードモナスｋｗｉ―４菌株
JPH0838172A (ja)	1996-02-13	新規β−アガラーゼ及びその製造方法
JPH08275776A (ja)	1996-10-22	新規キチナーゼ及びその製造方法
JPH0466083A (ja)	1992-03-02	α―グルコシダーゼおよびその製造法
EP0187869A1 (en)	1986-07-23	Process for preparing lipase
Kambourova et al.	1987	Biosynthesis and properties of extracellular pullulanase from Bacillus stearothermophilus G-82
JPH0257183A (ja)	1990-02-26	新規ヘパラン硫酸分解酵素並びにそれを生産する微生物及び方法
JPH0425794B2 (cs)	1992-05-01
JPH0561911B2 (cs)	1993-09-07
JPS6363382A (ja)	1988-03-19	キトサナ−ゼの製造法
Kambourova et al.	1987	BAS, 1113 Sofia, Bulgaria
JPH0937773A (ja)	1997-02-10	ムタナーゼ産生微生物及びムタナーゼ
JPS62155085A (ja)	1987-07-10	Ｌ−フコ−ス・デヒドロゲナ−ゼの製造法