JPH0564733B2 - - Google Patents

Info

Publication number: JPH0564733B2
Authority: JP; Japan
Prior art keywords: enzyme; biosensor; electrode; sensitive surface; sensitive
Prior art date: 1984-11-16
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Lifetime

Application number

JP59242744A

Other languages

English (en)

Japanese (ja)

Other versions

JPS61120054A (ja

Inventor

Shotaro Oka

Osamu Tawara

Hiroyoshi Mizuguchi

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Shimadzu Corp

Original Assignee

Shimadzu Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1984-11-16

Filing date

1984-11-16

Publication date

1993-09-16

1984-11-16 Application filed by Shimadzu Corp filed Critical Shimadzu Corp

1984-11-16 Priority to JP59242744A priority Critical patent/JPS61120054A/ja

1986-06-07 Publication of JPS61120054A publication Critical patent/JPS61120054A/ja

1993-09-16 Publication of JPH0564733B2 publication Critical patent/JPH0564733B2/ja

Status Granted legal-status Critical Current

Links

108090000790 Enzymes Proteins 0.000 claims description 25
102000004190 Enzymes Human genes 0.000 claims description 25
239000012528 membrane Substances 0.000 claims description 23
108010025899 gelatin film Proteins 0.000 claims description 15
229910000000 metal hydroxide Inorganic materials 0.000 claims description 15
150000004692 metal hydroxides Chemical class 0.000 claims description 15
239000000126 substance Substances 0.000 claims description 15
108010093096 Immobilized Enzymes Proteins 0.000 claims description 14
150000001875 compounds Chemical class 0.000 claims description 7
229910003480 inorganic solid Inorganic materials 0.000 claims description 7
239000013522 chelant Substances 0.000 claims description 6
229910052751 metal Inorganic materials 0.000 claims description 6
239000002184 metal Substances 0.000 claims description 6
150000004703 alkoxides Chemical class 0.000 claims description 4
230000005669 field effect Effects 0.000 claims description 4
125000002524 organometallic group Chemical group 0.000 claims description 4
230000007062 hydrolysis Effects 0.000 claims description 3
238000006460 hydrolysis reaction Methods 0.000 claims description 3
239000003446 ligand Substances 0.000 claims description 3
229940088598 enzyme Drugs 0.000 description 22
239000000243 solution Substances 0.000 description 8
238000006911 enzymatic reaction Methods 0.000 description 6
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 5
239000004202 carbamide Substances 0.000 description 5
238000005259 measurement Methods 0.000 description 5
KRHYYFGTRYWZRS-UHFFFAOYSA-N Fluorane Chemical compound F KRHYYFGTRYWZRS-UHFFFAOYSA-N 0.000 description 4
YRKCREAYFQTBPV-UHFFFAOYSA-N acetylacetone Chemical compound CC(=O)CC(C)=O YRKCREAYFQTBPV-UHFFFAOYSA-N 0.000 description 4
230000003100 immobilizing effect Effects 0.000 description 4
150000002500 ions Chemical class 0.000 description 4
238000000034 method Methods 0.000 description 4
BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 4
239000000523 sample Substances 0.000 description 4
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
108010046334 Urease Proteins 0.000 description 3
239000002253 acid Substances 0.000 description 3
239000007864 aqueous solution Substances 0.000 description 3
239000011521 glass Substances 0.000 description 3
XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 3
239000004065 semiconductor Substances 0.000 description 3
239000002904 solvent Substances 0.000 description 3
WYTZZXDRDKSJID-UHFFFAOYSA-N (3-aminopropyl)triethoxysilane Chemical compound CCO[Si](OCC)(OCC)CCCN WYTZZXDRDKSJID-UHFFFAOYSA-N 0.000 description 2
SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 2
239000002262 Schiff base Substances 0.000 description 2
150000007513 acids Chemical class 0.000 description 2
239000007822 coupling agent Substances 0.000 description 2
238000001035 drying Methods 0.000 description 2
230000000694 effects Effects 0.000 description 2
125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
230000002452 interceptive effect Effects 0.000 description 2
239000000203 mixture Substances 0.000 description 2
229920000620 organic polymer Polymers 0.000 description 2
239000008363 phosphate buffer Substances 0.000 description 2
229910052697 platinum Inorganic materials 0.000 description 2
230000035945 sensitivity Effects 0.000 description 2
239000010936 titanium Substances 0.000 description 2
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
YRAJNWYBUCUFBD-UHFFFAOYSA-N 2,2,6,6-tetramethylheptane-3,5-dione Chemical compound CC(C)(C)C(=O)CC(=O)C(C)(C)C YRAJNWYBUCUFBD-UHFFFAOYSA-N 0.000 description 1
-1 3-phenyl- 2,4-pentanedione (3-phenylacetylacetone) Chemical compound 0.000 description 1
OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
108010015133 Galactose oxidase Proteins 0.000 description 1
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
108010015776 Glucose oxidase Proteins 0.000 description 1
239000004366 Glucose oxidase Substances 0.000 description 1
239000004367 Lipase Substances 0.000 description 1
102000004882 Lipase Human genes 0.000 description 1
108090001060 Lipase Proteins 0.000 description 1
229930182555 Penicillin Natural products 0.000 description 1
JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
108010087702 Penicillinase Proteins 0.000 description 1
108010020346 Polyglutamic Acid Proteins 0.000 description 1
ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
150000004753 Schiff bases Chemical class 0.000 description 1
229910052581 Si3N4 Inorganic materials 0.000 description 1
229910004298 SiO 2 Inorganic materials 0.000 description 1
239000006087 Silane Coupling Agent Substances 0.000 description 1
VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
BOTDANWDWHJENH-UHFFFAOYSA-N Tetraethyl orthosilicate Chemical compound CCO[Si](OCC)(OCC)OCC BOTDANWDWHJENH-UHFFFAOYSA-N 0.000 description 1
RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
PNNCWTXUWKENPE-UHFFFAOYSA-N [N].NC(N)=O Chemical compound [N].NC(N)=O PNNCWTXUWKENPE-UHFFFAOYSA-N 0.000 description 1
230000004913 activation Effects 0.000 description 1
230000002411 adverse Effects 0.000 description 1
125000003172 aldehyde group Chemical group 0.000 description 1
WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
229910052782 aluminium Inorganic materials 0.000 description 1
XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
238000004458 analytical method Methods 0.000 description 1
239000002585 base Substances 0.000 description 1
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
239000012490 blank solution Substances 0.000 description 1
239000007853 buffer solution Substances 0.000 description 1
239000011575 calcium Substances 0.000 description 1
229910052791 calcium Inorganic materials 0.000 description 1
239000000969 carrier Substances 0.000 description 1
229920002301 cellulose acetate Polymers 0.000 description 1
239000011248 coating agent Substances 0.000 description 1
238000000576 coating method Methods 0.000 description 1
230000008878 coupling Effects 0.000 description 1
238000010168 coupling process Methods 0.000 description 1
238000005859 coupling reaction Methods 0.000 description 1
ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
230000003247 decreasing effect Effects 0.000 description 1
238000001514 detection method Methods 0.000 description 1
238000010586 diagram Methods 0.000 description 1
230000007613 environmental effect Effects 0.000 description 1
229930182830 galactose Natural products 0.000 description 1
229920000370 gamma-poly(glutamate) polymer Polymers 0.000 description 1
239000008103 glucose Substances 0.000 description 1
229940116332 glucose oxidase Drugs 0.000 description 1
235000019420 glucose oxidase Nutrition 0.000 description 1
230000020169 heat generation Effects 0.000 description 1
238000010438 heat treatment Methods 0.000 description 1
NDOGLIPWGGRQCO-UHFFFAOYSA-N hexane-2,4-dione Chemical compound CCC(=O)CC(C)=O NDOGLIPWGGRQCO-UHFFFAOYSA-N 0.000 description 1
238000011065 in-situ storage Methods 0.000 description 1
229910052500 inorganic mineral Inorganic materials 0.000 description 1
239000003456 ion exchange resin Substances 0.000 description 1
229920003303 ion-exchange polymer Polymers 0.000 description 1
229910052742 iron Inorganic materials 0.000 description 1
229940040461 lipase Drugs 0.000 description 1
235000019421 lipase Nutrition 0.000 description 1
239000007788 liquid Substances 0.000 description 1
230000007774 longterm Effects 0.000 description 1
238000004020 luminiscence type Methods 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
239000011707 mineral Substances 0.000 description 1
230000007935 neutral effect Effects 0.000 description 1
TWNQGVIAIRXVLR-UHFFFAOYSA-N oxo(oxoalumanyloxy)alumane Chemical compound O=[Al]O[Al]=O TWNQGVIAIRXVLR-UHFFFAOYSA-N 0.000 description 1
239000002245 particle Substances 0.000 description 1
229940049954 penicillin Drugs 0.000 description 1
229950009506 penicillinase Drugs 0.000 description 1
229920000915 polyvinyl chloride Polymers 0.000 description 1
239000004800 polyvinyl chloride Substances 0.000 description 1
239000011591 potassium Substances 0.000 description 1
229910052700 potassium Inorganic materials 0.000 description 1
239000002994 raw material Substances 0.000 description 1
230000004043 responsiveness Effects 0.000 description 1
239000012488 sample solution Substances 0.000 description 1
HQVNEWCFYHHQES-UHFFFAOYSA-N silicon nitride Chemical compound N12[Si]34N5[Si]62N3[Si]51N64 HQVNEWCFYHHQES-UHFFFAOYSA-N 0.000 description 1
229910052814 silicon oxide Inorganic materials 0.000 description 1
239000007787 solid Substances 0.000 description 1
239000000758 substrate Substances 0.000 description 1
239000010409 thin film Substances 0.000 description 1
229910052719 titanium Inorganic materials 0.000 description 1
238000005406 washing Methods 0.000 description 1
229910052725 zinc Inorganic materials 0.000 description 1
239000011701 zinc Substances 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/403—Cells and electrode assemblies
- G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Molecular Biology (AREA)
Physics & Mathematics (AREA)
Analytical Chemistry (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
Zoology (AREA)
Immunology (AREA)
General Physics & Mathematics (AREA)
Biophysics (AREA)
Pathology (AREA)
Biotechnology (AREA)
Microbiology (AREA)
Electrochemistry (AREA)
Chemical Kinetics & Catalysis (AREA)
Microelectronics & Electronic Packaging (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

JP59242744A 1984-11-16 1984-11-16 バイオセンサ装置 Granted JPS61120054A (ja)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
JP59242744A JPS61120054A (ja)	1984-11-16	1984-11-16	バイオセンサ装置

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP59242744A JPS61120054A (ja)	1984-11-16	1984-11-16	バイオセンサ装置

Publications (2)

Publication Number	Publication Date
JPS61120054A JPS61120054A (ja)	1986-06-07
JPH0564733B2 true JPH0564733B2 (fr)	1993-09-16

Family

ID=17093609

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP59242744A Granted JPS61120054A (ja)	1984-11-16	1984-11-16	バイオセンサ装置

Country Status (1)

Country	Link
JP (1)	JPS61120054A (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP2011220803A (ja) *	2010-04-08	2011-11-04	Mitsumi Electric Co Ltd	電界効果トランジスタ素子を具備するバイオセンサ

1984
- 1984-11-16 JP JP59242744A patent/JPS61120054A/ja active Granted

Also Published As

Publication number	Publication date
JPS61120054A (ja)	1986-06-07

Publication	Publication Date	Title
Pijanowska et al.	1997	pH-ISFET based urea biosensor
Van der Schoot et al.	1987	ISFET based enzyme sensors
Shul'Ga et al.	1994	Thin-film conductometric biosensors for glucose and urea determination
Karbue et al.	1986	A microsensor for urea based on an ion-selective field effect transistor
Hendji et al.	1993	Sensitive detection of pesticides using a differential ISFET-based system with immobilized cholinesterases
Vianello et al.	1996	Potentiometric detection of formaldehyde in air by an aldehyde dehydrogenase FET
Gotoh et al.	1986	A microsensor for adenosine-5′-triphosphate pH-sensitive field effect transistors
Lee et al.	2000	Microfabricated conductometric urea biosensor based on sol‐gel immobilized urease
Shul'ga et al.	1992	Overall characterization of ISFET-based glucose biosensor
Beyer et al.	1994	Development and application of a new enzyme sensor type based on the EIS-capacitance structure for bioprocess control
JPS62232555A (ja)	1987-10-13	酵素センサ
EP1561100A1 (fr)	2005-08-10	Biocapteurs bases sur un transistor presentant des electrodes de grille revetues de molecules receptrices
Ltith et al.	2001	Penicillin detection by means of silicon-based field-effect structures
Mourzina et al.	2004	Immobilization of urease and cholinesterase on the surface of semiconductor transducer for the development of light-addressable potentiometric sensors
Bousse et al.	1990	Biosensors for detection of enzymes immobilized in microvolume reaction chambers
Korpan et al.	1997	A Novel Enzyme Biosensor Specific for Formaldehyde Based on pH‐Sensitive Field Effect Transistors
Vlasov et al.	1991	Enzyme semiconductor sensor based on butyrylcholinesterase
Vlasov et al.	1992	Analytical applications of pH-ISFETs
Battilotti et al.	1989	Characterization of biosensors based on membranes containing a conducting polymer
US20070001253A1 (en)	2007-01-04	Using extended gate field effect transistor to design and analyze the chinese medicine biosensor
JPH0564732B2 (fr)	1993-09-16
De Melo et al.	2003	Use of competitive inhibition for driving sensitivity and dynamic range of urea ENFETs
JPH0564733B2 (fr)	1993-09-16
Colapicchioni et al.	1992	Fabrication and characterization of ENFET devices for biomedical applications and environmental monitoring
JP2862940B2 (ja)	1999-03-03	固定化酵素およびそれを用いる測定装置