JPH0595732A - Method for grafting seedling - Google Patents

Abstract

PURPOSE:To shorten a grafting period, improve the rooting rate of grafted seedlings and massively obtain the healthy grafted seedlings by grafting a scion to the stock of the seedling and subsequently taking care of the grafted seedling until the grafted part is rooted while the temperature, humidity, illuminance, etc., of the device are controlled. CONSTITUTION:A scion 2 otherwise grown is grafted to the stock 1 of a seeding, and the grafted part is held with a holder 4 in the grafted state. The stock 1 in the grafted state is carried into a rooting-accelerating device whose temperature, humidity, illuminance, etc., are controlled, and subsequently taken care until the grafted part is rooted, while the temperature, humidity, illuminance, and CO2 content of the device are controlled preferably to 25-30 deg.C, 70-100%, 3000-10000lux and 600-1200ppm, respectively, thereby permitting to improve the operation rate of the rooting-accelerating device and extremely reduce the production cost of the seedling.

Description

Detailed Description of the Invention

[0001]

[Field of Industrial Application] The present invention is tomato, eggplant, pepper, watermelon, melon, cucumber, carnation, Kasumi grass,
Fruit vegetables such as chrysanthemums, or herbaceous plants such as flowers, and roses,
TECHNICAL FIELD The present invention relates to a method for grafting flowering trees such as lilacs, grapes and apples, or woody plants such as fruit trees, or viruses for tissue culture seedlings or sweet potatoes, strawberries, chrysanthemums etc. for viroid test.

[0002]

2. Description of the Related Art Generally, it is already known to produce seedlings by grafting for the purpose of disease control, imparting robustness, breeding of vegetative breeding plants that are difficult to cut, and in recent years,
Demand for grafting seedlings is increasing due to the expansion of scale, progress of professionalization, and aging of growers, but current grafting technology must meet the above demand both in terms of quantity and cost. It is difficult.

Recently, a lot of free seedlings such as viruses have been produced by tissue culture, but the grafting method may be used for assaying these viruses, and an efficient and good grafting method for this purpose. There is no such thing.
And, as the grafting of the present seedlings, call grafting, split grafting, cutting grafting, mating grafting, cutting grafting and the like are performed.

[0004]

DISCLOSURE OF INVENTION Problems to be Solved by the Invention The grafting method requires that the rootstock and the scion have to be engaged with each other, inserted, joined, etc., and that the stem is flexible, and so on. Until it becomes thick to some extent, for example, tomato, eggplant, etc., about 5 leaf stage, cucumber, watermelon, etc. until 1 leaf stage,
In addition, since woody plants such as roses and apples have to be cultivated until the roots of the rootstock become almost woody, it takes a long time before the grafting time and the operation is troublesome.
Moreover, in the herbaceous plants, since the spikes were rather heavy, the yield was poor, for example, the joints peeled off during the curing period and the spikes died. Furthermore, in the case of woody plants, it takes a lot of time to match the growth stages of a large amount of scion and rootstock, and when both are used as grafts that do not match the stage, the survival rate becomes low. There were many.

Furthermore, vegetatively propagating plants such as sweet potatoes (spikes are morning glory), strawberries (rootstocks are wild strawberries), chrysanthemums (spikes are mistletoe) are propagated by tissue culture for the purpose of making them virus- or viroid-free. In doing so, it becomes necessary to test whether or not the plant once cultivated in the field is contaminated with virus or viroid during the process of mass-proliferation of cultured seedlings. At this time, in some plants, the target plant is a rootstock (or rootstock), and the closely related test plants are rootstocks (or rootstocks).
As a result, the test plant is tested for whether or not any symptom indicating virus or viroid contamination occurs. Although this grafting is split grafting and mating grafting, it is difficult to systematically certify these grafts by these methods, so systematic verification was difficult.

[0006]

[Means for Solving the Problems] Separately grown spikes are joined to a rootstock of a grown seedling, the joint is held by a support, and then the seedling promotion device is controlled in temperature, humidity, light amount, and the like. The above-mentioned problems were solved by advancing the grafting time and improving the survival rate by carrying in and curing and carrying out growth until at least the above-mentioned joints have become active.

[0007]

[Action] Rootstock and scion of seedlings are trained. The rootstock of this seedling is cut with a thin and sharp cutter such as a razor blade, and the spikelets are also cut, and the spikelets are joined to the rootstock with their cut surfaces parallel to each other, and this joint is held. The joint state is kept constant by. Immediately after producing a large number of such grafted seedlings, the seedlings are immediately carried into a survival promoting device capable of controlling temperature, humidity, light amount, and the like, and preferably the temperature in the survival promoting device is 25 to 30 ° C. and the humidity is 70 to 70 ° C. 100%, light quantity 3000 to 10000 Lx, CO ₂ 600 to 120
Grow at 0 ppm until at least the joint is alive.

[0008]

【Example】

Embodiment (1) An embodiment of the present invention will be described with reference to the drawings of a tomato graft. Homarare 114, Momotaro,
House Momotaro, KNVF R-3, joint, BF Okitsu 101, disease-resistant Shinko No. 1 etc. are used as the rootstock, and the rootstock raising container is an appropriate tray 3, for example, a flat shape of 51 × 30.
cm with 51 holes and each hole has a surface area of 17c
m ^2, depth 5.5cm, used as volume 67 ml, rootstocks 1, the culture soil was inoculated and filled in each well, in this leaves about 2.5 leaf (average value in a greenhouse, seedlings Length 5.5 cm, number of leaves 2.
Three seedlings were grown to a stem diameter of 1.8 mm).

The scion 2 was flat-sown in a nursery box until the true leaves were about 2.5 sheets like the rootstock 1, or was transplanted and grown in a greenhouse at about the same time as the rootstock 1. .. When the rootstock 1 and the scion 2 are in the grafting time as described above, the rootstock 1 is placed as a group 3 seedlings on the workbench at once in the tray 3, and the upper part of the cotyledon of each seedling is cut by a safety razor blade. Cut diagonally at an angle of about 60 degrees.

Next, the elastic material tubular holder 4 made of rubber or synthetic resin is fitted to the cut end of the base stock 1. This holder 4 has a large number of grooves in the lengthwise direction of the outer periphery and one slit 5, and the upper and lower end faces may be perpendicular to the lengthwise direction but are tilted in the same direction. By matching and fitting the cut surface of the tree 1, the cut surface of the spike 2 is matched with the inclination direction of the upper end of the holder 4 and only inserted from the upper end of the holder 4 Becomes parallel to the cut surface of the rootstock 1, and both cut surfaces can be joined very easily and closely. In addition, the stem diameter of the rootstock 1 and the scion 2 is 1
Grafting is possible even on the order of mm. When the grafting of all the trays 3 is completed in this way, the trays 3 as they are are carried into the shelf in the survival promotion device 10.

Next, the survival promoting device 10 will be described with reference to FIG. 3. The heat insulating outer structure 11 having a doorway that can be hermetically sealed.
A box-shaped heat insulating inner structure 15 having opposite side walls of a blown perforated plate 12 and a sucked perforated plate 13 is provided inside the air passage 16 and the pressurizing chamber 17 so as to separate the air passage 16 from one side lower part. Is provided with a cooler 18, an electric heater 19 and a humidifier 20, and between the end of the air passage 16 and the upper portion of the pressurizing chamber 17, a plurality of blower fans 8 ... , Ventilation holes 21 and 22 are provided on the suction side and the discharge side of the heat insulating exterior 11,
The CO ₂ gas in the O ₂ cylinder 28a is mixed with the air whose temperature and humidity are controlled by being ejected from the suction side nozzle 28b of the blower fan 8 through the electromagnetic control valve 23.

The control panel 24 has a built-in microcomputer, and has a temperature controller 25, a humidity controller 26, a temperature control switch 27, and C.
The control panel is equipped with an O ₂ switch 28, a wind force controller 29, etc., and the temperature and humidity depend on the detected values of the temperature / humidity sensor 25a and the temperature upper limit thermostat 25b and the set values of the temperature controller 25 and the humidity controller 26. The output from 24 operates the cooler 18 or the electric heater 19 and the humidifier 20 to control the room temperature and humidity, and the CO ₂ concentration is measured by the CO ₂ monitor 3.
The electromagnetic control valve 2 is set according to the detected value of 0 and the set value of the control panel 24.
The wind speed is controlled by adjusting the rotation speed of the blower fan 8 by the wind force controller 29.

The temperature, humidity and wind speed are set according to the type of plant to be cured, the curing period, etc., but the temperature is 25 to 30 ° C., the humidity is 70 to 100%, and the CO _{2 is} 600 to 600.
Controlled in the range of 1200 ppm and wind speed of 30 to 80 cm / sec, the holes of the suction perforated plate 12 and the blowout perforated plate 13 have different diameters or arrangement intervals depending on the positions so that the wind speed of any part of the heat insulating inner structure 15 is substantially uniform. However, the area of the holes may be adjustable. A shelf frame 33 having a plurality of shelves 33a is housed in the heat insulating inner structure 15, and a large number of fluorescent lamps or other lighting fixtures 35 are provided on the lower surfaces of the second or more shelves 33a and the top plate 34.・ ・ Is attached, and the illuminance is 3000 to 1000 depending on the number of operating lighting fixtures 35 or voltage control.
Control in the range of 0Lx.

In curing the tomato grafted seedlings, the above-mentioned grafted seedlings are carried in together with the tray 3 and each shelf board 33a.
Arranged on ..., Temperature: 28 ° C, humidity: 85 to 95, illuminance: 5000Lx, CO ₂ concentration: 600～900Pp
When cured for 4 days while controlling m, wind speed: 50 to 70 cm / sec, on average, plant height: 11.6 cm, number of leaves: 4.9, stem diameter: 3.6 mm. Even when paying attention while carrying in the above-mentioned carry-in, the grafted seedling sways, but since the scion 2 is very young and light,
The joint surface with the rootstock 1 did not separate or shift. The following table shows the actual results comparing the curing under the above conditions with the conventional curing performed in the greenhouse.

[0015]

As is clear from the above experimental results, the survival rate, the plant height showing growth, the number of leaves, the fresh weight, and the cotyledon growth rate showing seedling growth are all significant compared with the conventional grafting method. The difference was obtained. Note that the holder 4 does not need to be removed because the slits open and the holder 4 naturally falls when the stem of the seedling becomes thick. Further, fruit vegetables other than tomatoes can be implemented by almost the same method.

Example (2) Carnation (rootstock is Fuji Nadesico, beauty Nadesico etc.), rose (rootstock is Neubara, R. odorata etc.),
Flowering trees such as lilac (rootstock is Ibota), and grapes (rootstock is pest-resistant grapes), apples (rootstock is Maruba Kaido), cherries (rootstock is Aobazakura), pears (rootstock is quince) , 20 to 20% of the stems or green branches of rootstock that are relatively easy to root when cutting, such as pears
Cut with a sharp cutter to a length of cm, cut each desired cultivar on the top with a sharp cutter to a length of 2 to 10 cm from the start of the bud, and join them as a scion.
The grafted seedlings are kept in a joined state by means of cutting, and the grafted seedlings are inserted into a tray filled with the soil as much as possible, and the tray is left as it is and carried into a rooting promotion device in which temperature, humidity, illuminance and wind speed are controlled. At the same time as rooting is promoted, the joint part is made alive.

Example (3) In the woody plant of the above Example (2), cherry blossoms (rootstock is Kaido), magnolia (rootstock is fist), flower dogwood (rootstock is flower dogwood / flower color and other characteristics) Ordinary ones and so on), plums (rootstocks are plums), peaches (rootstocks are peaches), bayberry (rootstocks are bayberry), roeby (rootstocks are roby), and tangerines (stands) Trees are citrus fruits such as Karachi, peach (rootstock is peach), persimmon (rootstock is mamegaki), plum (rootstock is plum), apricot (rootstock is apricot). For rootstocks that take days to root, once the green branches to be rootstock are 5 to 20 cm.
Cut the length of this green branch and put this green branch in a tray filled with cultivating soil as much as possible and darken (it is enough to cover with black vinyl) Days required for high humidity conditions (mostly 1 month or more) ), Prepare a well-rooted plant in advance, cut the upper part again with a sharp cutter at the time of grafting, and put each desired cultivar on the upper part 2-10 cm from the tip of the bud
Cut with a sharp cutter to the length of
The bonded state is held by the holder 4, and the grafted seedlings are carried in the tray as they are in a tray and cured in a survival promoting apparatus in which the temperature, humidity, light amount, etc. are controlled.

Example (4) For sweet potatoes, strawberries, and chrysanthemums, seedlings that had been budded or planted in a tray in advance were used as rootstocks, and the middle of the stem or petiole was cut with a sharp cutter, and the tip of each bud was cut off. 2-10 cm
Cut a petiole with a stem or leaf of a length of 1 by a sharp cutter, join this as a scion, and hold the joined state by the holder 4, keeping these grafted seedlings in the tray at temperature,
Carry it in and bring it to a survival promotion device that controls humidity, light intensity, wind speed, etc.

In the above-mentioned embodiment, the size of the rootstock, the period in the curing step, the illuminance, the humidity, the temperature, the wind speed, etc. are set according to the kind of seedling.

[0021]

INDUSTRIAL APPLICABILITY According to the present invention, a rooting 1 for seedlings is joined to a spike 2 which has been separately grown, and the joined portion is held in a joined state by a holder 4, and then the rooting is performed to control the temperature, humidity, light amount, etc. Since the curing is carried in into the promotion device 10 and the above control is performed until at least the joint portion is set alive, the grafting time can be advanced and the curing period can be shortened. The operation rate can be improved, the production cost of seedlings can be remarkably reduced, mass production can be performed by a small-scale facility, and long-distance transportation can be performed without damaging a large amount of seedlings.

[Brief description of drawings]

FIG. 1 is a front view of a grafted seedling.

FIG. 2 is an enlarged view of an adhesive portion of a graft.

FIG. 3 is a layout view of a survival promotion device.

FIG. 4 is a front view showing an example in which a grafted graft is grafted.

FIG. 5 is a perspective view showing conventional cutting and joining (spring) of apple seedlings.

FIG. 6 is a perspective view showing the above-mentioned green branch cutting joint. 1 rootstock 2 scion 3 tray 4 holder 8 blower fan 10 survival promotion device 12 perforated plate 13 perforated plate 16 air duct 33 shelves

─────────────────────────────────────────────────── ───

[Procedure amendment]

[Submission date] June 5, 1991

[Procedure Amendment 1]

[Document name to be amended] Statement

[Correction method] Change

[Correction content]

Since carnation has low heat resistance, in normal climate cultivation, which is the most common type of cultivation in Japan, planting is carried out in May to June avoiding the harvest in summer and from October to the end of May to June of the following year. Harvested until the beginning of the year. At present, cultivation by carnation grafting is not carried out, but by using dianthus with excellent heat resistance and robustness as rootstock and carnation grafting as scion, the scion also has heat resistance and robustness. It is possible to extend the harvest period from June to July, and it is industrially useful because a new cropping pattern can be established for the production during the summer shortage period when only a small amount of cut flowers produced in cool regions are circulated. .. Example (2) When cutting and grafting are performed at the same time, the rootstock has affinity with carnation in the same Dianthus genus,
Those that are heat resistant, strong, and have relatively thick stems, such as Visiona deco (also known as American Dianthus; Dia)
nthus barbatus L. ) And Fujina Desico (aka Hamanadeshiko; Dianthus japon)
icus Thunb. ) Or the like is preferably used.
About 9 cm long, the tip of the vigorous buds was cut about 10 cm long in the previous year, and the roots were set by rooting, and when rooted, they were planted in No. 5 plastic pots or planters and used as the rootstock for the rootstock. As a large stock, grow a large number of vibrant axillary buds. Approximately from February to April, the shoots that have grown to about 10 cm are picked up, and the stems at the positions where the internodes at the base have spread to about 2 cm are lengthened with a sharp blade (such as a razor blade made of stainless steel). Cut it so that its cross section is smooth by sharpening it so that it is about 30 degrees to the direction. The holder 4 is fitted and placed on the thus-cut tip. The scion may be any practical carnation variety to be cultivated, scraping axillary buds generated during the cultivation of virus-free (healthy) strains, and smoothing the cross section of the stem near the base with a sharp knife at an angle of 30 degrees. Cut carefully so that The cut cuttings are inserted into the holes of the holder 4 in the same direction as the cutting surface of the rootstock, and the cutting surfaces are sufficiently pressure-bonded. After joining, insert the rootstock into the tray 3 with sterilized vermiculite as a soil, temperature 25 ° C, humidity 95%, light intensity 500
The product is stored in the survival promotion device 10 controlled at 0 to 10000 LX and a wind speed of 30 cm / sec, and cured for 7 to 15 days. When it is lived, it is taken out and raised in a normal nursery room. If it is desired to shorten the number of storage days in the device to some extent, a rooting promoter such as auxin may be applied to the soil in which the rootstock is inserted. If you want to shorten the number of days required for storage, insert only the rootstock once and keep it under high humidity conditions in a normal greenhouse for about 10 days.
It is also possible to cut with a sharp blade at 0 °, carry out the grafting operation there as described above, and then store it in the survival promotion device 10.
With these, 95% or more of live seedlings can be obtained.

[Procedure Amendment 2]

[Document name to be amended] Statement

[Correction target item name] 0018

[Correction method] Change

[Correction content]

Example (3) Woody plants such as cherry blossoms (rootstock is Kaido), magnolias (rootstock is fist), flower dogwood (rootstock is flower dogwood, flower color and other characteristics are normal. Same), plum (rootstock is plum), peach (rootstock is peach), bayberry (rootstock is bayberry), roubais (rootstock is roby), and mandarin oranges (rootstock is citrus fruits such as karachi) ), Peach (rootstock is peach), persimmon (rootstock is mamegaki), plum (rootstock is plum), apricot (rootstock is apricot) and other fruit trees that take many days to root during cutting. In the case of trees, the green branches to be used as the rootstock are once cut into a length of 5 to 20 cm, and the green branches are cut into a tray filled with soil as much as possible and dark (covered with black vinyl is enough). Prepare the well-rooted plants in advance for the required number of days (mostly one month or more) for high humidity conditions, and re-create them when grafting. The upper part is cut with a sharp cutter, and each desired cultivar is cut on the upper part with a sharp cutter to a length of 2 to 10 cm from the tip of the sprout, joined as a spike, and joined by a holder 4. Then, the grafted seedlings are carried in the tray as they are in a tray and cured in a survival promoting apparatus in which the temperature, humidity, light amount, etc. are controlled.

[Procedure 3]

[Document name to be amended] Statement

[Name of item to be corrected] 0019

[Correction method] Change

[Correction content]

[0019] Sweet potatoes, which are vegetatively propagated, are infected with a virus while they have been passed down for many years, which damages the color of the roots (potato), roughens the epidermis, reduces the commercial viability, and increases the vigor. It weakens and causes a decrease in yield. It has been widely put into practical use in recent years by improving the quality and increasing the yield by tissue-cultivating this and making it virus-free, but even if virus-free seedlings are once used, they can be subcultured by farmers. In the meantime, the virus re-infects the seedlings, so it is necessary to update the seedlings to new ones that are not contaminated as long as the effect is not diminished. The sweet potato virus test is based on the morning glory Ipomoea setosa
As a scion, it depends on the method of grafting on the rootstock of sweet potato, but with the conventional grafting method, it is difficult to carry out the grafting operation, the survival / curing method, and the mass treatment, and the survival rate is 40-50. Since it is only about 10%, it was extremely insufficient as a method for testing the actual condition of infection at the cultivation site by the virus-free passaged seedlings that have been widely spread by tissue culture. Example (4) Around the time when the vine length of sweet potatoes to be tested for the presence or absence of virus morbidity, or the extent thereof, has grown to 15 to 20 cm or less,
Cut the top and bottom of the stems with scissors with expanded leaves and 2 leaves, and insert into a polyethylene pot containing sterilized culture soil of No. 2 (diameter 5 cm), or a connecting hole tray of the same size, greenhouse or net room When the axillary buds grew from the axils of each node to 4 to 5 cm or more, the stem of the tip of the node that extended to 1 to 2 cm between the nodes was slanted at 30 degrees and a sharp blade (razor blade). etc)
Then, cutting is performed so that the cross section becomes smooth, and the holder 4 is inserted so that the tilt direction is aligned with the same direction as the cross section. For virus test plants that become spikes, for example, morning glory'Ipomoea setosa ', seeds that have been cut into a seedling vat with scissors in advance to facilitate water absorption are directly sown at intervals of about 8 × 3 cm, and at 30 ° C. Germinated in C germination unit for 3 to 4 days, and the leaf height of bud-real leaves was about 3c.
When grown to about m, the hypocotyl 2-3 cm below the cotyledon,
Cut the rootstock at the same angle as the sweet potato, and insert it strongly into the hole of the holder 4 that was previously inserted into the sweet potato from above so that the inclination directions of the base and the spikes are aligned. The temperature of the seedling that has been contacted is 28 ° C, humidity is 95%, and light intensity is 5000 L
X, a survival promotion device 1 adjusted to a wind speed of 30 cm / sec
Carry it in 0 and let it cure for 4-5 days to fully root the joint. As a result, almost 100% of live seedlings can be obtained. When the vines have been settled, they are taken out into the greenhouse, the vines are grown according to the usual greenhouse management, and the presence or absence of virus contamination is judged by the yellowing symptom appearing in the true leaves of morning glory.

[Procedure correction 4]

[Document name to be amended] Statement

[Name of item to be corrected] Brief explanation of the drawing

[Correction method] Added

[Correction content]

FIG. 7 is a perspective view of a carnation graft.

FIG. 8 is a perspective view of a sweet potato cutting tree.

FIG. 9 is a perspective view of a sweet potato rootstock.

FIG. 10 is a perspective view of a spikelet (morning glory).

[Procedure amendment 5]

[Document name to be corrected] Drawing

[Correction method] Added

[Correction content]

[Figure 7]

[Figure 8]

[Figure 9]

[Figure 10] ─────────────────────────────────────────────────── ───

[Procedure amendment]

[Submission date] October 30, 1992

[Procedure Amendment 1]

[Document name to be amended] Statement

[Correction target item name] 0017

[Correction method] Change

[Correction content]

Since carnations have low heat resistance, in normal climate cultivation, which is the most common type of cultivation in Japan, planting is done from May to June avoiding the harvest in summer and from October to late May to early June. Harvested. At present, carnation grafting is not carried out, but the heat resistance and robustness are given to the scion by grafting carnation as a scion with dianthus that have excellent heat resistance and robustness. The harvesting period can be extended from June to July, and a new cropping pattern aimed at production during the summer shortage period in which only a small amount of cut flowers produced in cool regions are distributed is industrially useful. Example (2) When cutting and grafting are performed at the same time, the rootstock has affinity with carnation in the same Dianthus genus,
Those that are heat resistant, strong, and have relatively thick stems, such as Visiona deco (also known as American Dianthus; Dia)
nthus barbatus L. ) And Fujina Desico (aka Hamanadeshiko; Dianthus japon)
icus Thunb. ) And the like are preferably used.
About 9 cm long, the tip of the vigorous buds was cut about 10 cm long in the previous year, and the roots were set by rooting, and when rooted, they were planted in No. 5 plastic pots or planters and used as the rootstock for the rootstock. As a large stock, grow a large number of vibrant axillary buds. Approximately February-April, buds that have grown to about 10 cm are collected and picked, and the stem at the position where the internodes at the base extend to about 2 cm is cut with a sharp knife (such as a razor blade made of stainless steel). Cut it so that the cross section becomes smooth by sharpening it so that it is about 30 degrees to the depth direction. The holder 4 is fitted on the sharpened tip. The scion may be any practical carnation variety to be cultivated, scraping axillary buds generated during the cultivation of virus-free (healthy) strains, and smoothing the cross section of the stem near the base with a sharp knife at an angle of 30 degrees. Cut carefully so that The cut cuttings are inserted into the holes of the holder 4 in the same direction as the cutting surface of the rootstock, and the cutting surfaces are sufficiently pressure-bonded. After joining, insert the rootstock into the tray 3 with sterilized vermiculite as a soil, temperature 25 ° C, humidity 95%, light intensity 500
It is stored in the survival promotion device 10 controlled at 0 to 10000 LX and a wind speed of 30 cm / cec, and cured for 7 to 15 days. When it is lived, it is taken out and raised in a normal nursery room. If it is desired to shorten the number of storage days in the device to some extent, a rooting promoter such as auxin may be applied to the soil in which the rootstock is inserted. If you want to shorten the number of days required for storage, insert only the rootstock once and keep it under normal high humidity conditions in a greenhouse for about 10 days.
It is also possible to cut with a sharp blade at 0 °, carry out the grafting operation there as described above, and then store it in the survival promotion device 10.
With these, 95% or more of live seedlings can be obtained.

[Procedure correction 4]

[Document name to be amended] Statement

[Name of item to be corrected] Brief explanation of the drawing

[Correction method] Change

[Correction content]

[Brief description of drawings]

FIG. 1 is a front view of a grafted seedling.

FIG. 2 is an enlarged view of an adhesive portion of a grafted seedling.

FIG. 3 is a layout view of a survival promotion device.

FIG. 4 is a front view showing an example in which grafted grafts are grafted.

FIG. 5 is a perspective view showing conventional cutting and joining (spring) of apple seedlings.

FIG. 6 is a perspective view showing the above-mentioned green branch cutting joint.

FIG. 7 is a perspective view of a carnation graft.

FIG. 8 is a perspective view of a sweet potato cutting tree.

FIG. 9 is a perspective view of a sweet potato rootstock.

FIG. 10 is a perspective view of a spikelet (morning glory).

[Explanation of Codes] 1 Rootstock 2 Scrub 3 Tray 4 Retainer 8 Blower Fan 10 Survival Promotion Device 12 Perforated Plate 13 Perforated Plate 16 Airway 33 Shelf

[Procedure amendment 5]

[Document name to be corrected] Drawing

[Correction target item name] Figure 7

[Correction method] Added

[Correction content]

[Figure 7]

[Procedure Amendment 6]

[Document name to be corrected] Drawing

[Correction target item name] Figure 8

[Correction method] Added

[Correction content]

[Figure 8]

[Procedure Amendment 7]

[Document name to be corrected] Drawing

[Correction target item name] Figure 9

[Correction method] Added

[Correction content]

[Figure 9]

[Procedure Amendment 8]

[Document name to be corrected] Drawing

[Name of item to be corrected] Fig. 10

[Correction method] Added

[Correction content]

[Figure 10]

Claims (2)

[Claims] 1. A rooting 1 for seedlings is joined to a spiker 2 which has been separately grown, and the joined portion is held in a joined state by a holder 4, and then in a survival promoting device for controlling temperature, humidity, illuminance and the like. A method for grafting seedlings, which comprises carrying in and carrying out the above-mentioned control and curing until at least the joint portion is alive. 2. The temperature inside the survival promotion device is 25 to 30 ° C., the humidity is 70 to 100%, and the illuminance is 3000 to 1000.
The grafting method for seedlings according to claim 1, wherein 0 Lx and CO ₂ are controlled to 600 to 1200 ppm.