JPH0616559Y2 - Additive device for fermenter - Google Patents
Additive device for fermenterInfo
- Publication number
- JPH0616559Y2 JPH0616559Y2 JP1987035902U JP3590287U JPH0616559Y2 JP H0616559 Y2 JPH0616559 Y2 JP H0616559Y2 JP 1987035902 U JP1987035902 U JP 1987035902U JP 3590287 U JP3590287 U JP 3590287U JP H0616559 Y2 JPH0616559 Y2 JP H0616559Y2
- Authority
- JP
- Japan
- Prior art keywords
- valve
- line
- addition
- fermenter
- pot
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Description
【考案の詳細な説明】 〔産業上の利用分野〕 本考案は、発酵槽の添加物添加装置に係り、特にバイオ
ハザード対策設備の発酵槽に好適な添加物添加装置に関
するものである。DETAILED DESCRIPTION OF THE INVENTION [Industrial field of application] The present invention relates to an additive addition device for a fermenter, and more particularly to an additive addition device suitable for a fermenter of a biohazard countermeasure facility.
従来、発酵槽への添加物の添加は、例えば、火災投入法
により行われている。Conventionally, addition of additives to a fermenter has been performed by, for example, a fire charging method.
尚、この種の技術は、例えば、「発酵工業」、木下著、
大日本図書（株）昭和５０年７月１日発行、第９５頁に
おいて論じられている。In addition, this kind of technology is, for example, "Fermentation industry", by Kinoshita,
It is discussed on page 95 of Dainippon Books Co., Ltd., published on July 1, 1975.
上記従来技術は、添加物を添加（あるいは植菌）する場
合、その構造上、培養中の微生物や種菌中の微生物が周
囲の外気あるいは作業者を汚染するといった欠点を有
し、バイオハザード対策を行った環境の中で使用するこ
とが難しいといった問題を有している。特に、微生物が
周囲の生物系に対して危険なものである場合には、上記
従来技術は使用できない。When adding (or inoculating) an additive, the above-mentioned conventional technology has a drawback that microorganisms in culture or microorganisms in seeds contaminate the surrounding air or workers due to its structure, and biohazard countermeasures are required. It has a problem that it is difficult to use it in the environment where it went. In particular, the above-mentioned conventional techniques cannot be used when microorganisms are dangerous to surrounding biological systems.
本考案の目的は、バイオハザード対策を行った環境の中
で容易に使用できる簡潔構造の発酵槽の添加物添加装置
を提供することにある。An object of the present invention is to provide a fermenter additive adding device having a simple structure that can be easily used in an environment where biohazard measures are taken.
上記目的は、発酵槽の添加口を槽上部側壁に設け、該添
加口へ添加バルブ（５），カプラー（６）を介して添加
ラインを接続すると共に、添加バルブ（５）とカプラー
（６）間に蒸気バルブ（４）を有する蒸気ラインを接続
し、前記添加ラインの種菌用バルブ（７）の前段で分岐
させてその一方を種菌用バルブ（８），カプラー（６）
を介してドレンバルブ（１１）を有するドレンラインに
接続し、他方を種菌用バルブ（９）を介して発酵槽側方
の下方に設置したポットに接続される種菌移送ラインへ
接続し、前記ポットに空気圧送ラインを設け、該空気圧
送ラインにフィルター（１０），バルブ（３）を介して
空気ラインを接続して発酵槽の添加物添加装置を構成し
たことにより達成される。The purpose is to provide an addition port of the fermentation tank on the side wall of the upper part of the tank, connect the addition line to the addition port through the addition valve (5) and the coupler (6), and add the addition valve (5) and the coupler (6). A steam line having a steam valve (4) is connected between them, and is branched at a stage preceding the inoculum valve (7) of the addition line, and one of them is branched to inoculate valve (8) and coupler (6).
And a drain line having a drain valve (11), and the other through a valve (9) for inoculum to a inoculum transfer line connected to a pot installed below the side of the fermenter. This is achieved by providing an air pressure feed line to the air feed line and connecting the air line to the air pressure feed line via a filter (10) and a valve (3) to configure an additive addition device for a fermenter.
添加ラインを、蒸気供給ラインから供給される蒸気によ
り殺菌した後、空気圧送ラインから送られる空気により
ポットの内容物を圧送すれば、培養中の微生物又は植菌
中の微生物は配管等から周囲にもれることはなくなるの
で、周囲を微生物で汚染することは無い。After sterilizing the addition line with steam supplied from the steam supply line, if the contents of the pot are pressure-fed by the air sent from the air pressure feed line, microorganisms in culture or microorganisms inoculated into the surroundings from pipes etc. Since it will not leak, it will not contaminate the surrounding area with microorganisms.
以下、本考案の一実施例を第１図により説明する。 An embodiment of the present invention will be described below with reference to FIG.
発酵槽１上部の添加口１６には、添加バルブ５を介して
添加ライン１７が接続されている。添加ライン１７に
は、蒸気バルブ４を介して蒸気ライン１２が、またドレ
ンバルブ１１を介してドレンライン１４が接続されてい
る。ポット２には、バルブ９を介して、この場合、種菌
移送ライン１８と、殺菌用バルブ７と８が備えてある。
さらにポット２には、フィルター１０を介して空気圧送
ライン１３が接続されている。空気圧送ライン１３に
は、バルブ３を介して空気ライン１９が接続されてい
る。An addition line 17 is connected to the addition port 16 above the fermenter 1 via an addition valve 5. A steam line 12 is connected to the addition line 17 via a steam valve 4, and a drain line 14 is connected to the addition line 17 via a drain valve 11. The pot 2 is provided with a seed germ transfer line 18 and sterilization valves 7 and 8 in this case via a valve 9.
Further, an air pressure feed line 13 is connected to the pot 2 via a filter 10. An air line 19 is connected to the air pressure feeding line 13 via the valve 3.
上述の構成において例えば、種菌の発酵槽１への植菌に
ついて説明する。まず、あらかじめオートクレーブ（図
示せず）等で殺菌されたポット２、バルブ７〜９、ライ
ン１３，１８，１７、カプラー６、フィルター１０より
なるポットユニット２を安全キャビネット（図示せず）
の中に入れる。In the above configuration, for example, inoculation of the inoculum into the fermenter 1 will be described. First, a pot unit 2 including a pot 2, valves 7 to 9, lines 13, 18, and 17, a coupler 6, and a filter 10 that has been sterilized in advance by an autoclave (not shown) or the like is placed in a safety cabinet (not shown).
Put in.
つぎに、ポット２のフタ２０を開けて、ポット２内に種
菌を入れ、フタ２０を閉じる。バルブ７〜９が閉じてい
ることを確かめてから、ポットユニット２を安全キャビ
ネットより取り出し、カプラー６を、バルブ７側のもの
は蒸気ライン１２側へ、バルブ８側のものはドレンライ
ン１４側へ接続する。また、フィルター１０を空気ライ
ン１４へ接続する。この後に、バルブ４，７，８，１１
を開いて、蒸気を通して添加ライン１７を殺菌して、つ
いで、バルブ４を閉じ、バルブ５を開いて発酵槽１内の
無菌空気を供給して、添加ライン１７を冷却する。冷却
完了後バルブ８，１１を閉じ、バルブ９を開け、ついで
バルブ３を開けて、ポット２内に無菌空気を供給して加
圧すれば、ポット２内の種菌は、添加ライン１７を経て
発酵槽１へ圧送される。発酵槽１への植菌が終了する
と、上述と同様に、添加ライン１７の殺菌を行ったあ
と、カプラー６を離脱させてポットユニット２を発酵槽
１より取り外す。Next, the lid 20 of the pot 2 is opened, the inoculum is put in the pot 2, and the lid 20 is closed. After confirming that the valves 7 to 9 are closed, take out the pot unit 2 from the safety cabinet and put the coupler 6 on the valve 7 side to the steam line 12 side, and on the valve 8 side to the drain line 14 side. Connecting. Also, the filter 10 is connected to the air line 14. After this, valves 4, 7, 8, 11
Is opened to sterilize the addition line 17 by passing steam, and then the valve 4 is closed and the valve 5 is opened to supply sterile air in the fermenter 1 to cool the addition line 17. After completion of cooling, the valves 8 and 11 are closed, the valve 9 is opened, the valve 3 is then opened, and sterile air is supplied into the pot 2 to pressurize the inoculum in the pot 2 through the addition line 17 to ferment. It is pumped to tank 1. When the inoculation of the fermenter 1 is completed, the addition line 17 is sterilized, and then the coupler 6 is detached and the pot unit 2 is detached from the fermenter 1 as described above.
上記操作は、種菌に代えて添加物を用いれば、添加物添
加操作となる。The above operation becomes an additive addition operation if an additive is used instead of the inoculum.
本実施例によれば、種菌中の微生物が周囲の外気あるい
は作業者を汚染することがなく、バイオハザード対策を
行った環境の中で良好に使用できる。また、植菌操作中
種菌を完全に無菌的に保持することができる。また、発
酵槽の添加口を槽上部側壁に設け、ポットを発酵槽側方
の下方に設置しているため、操作が容易であり、ポット
を床上等に配置できる。このため、容量制限もなくな
り、液料の大きなポットも容易に脱着できる。他方、ポ
ットに（無菌）フィルターを設けて入るため、新たに無
菌空気ラインを設ける必要もない。さらに、添加口に蒸
気バルブを設けて入るため、添加後の添加ラインの殺菌
が容易にでき、殺菌後の添加ラインの冷却を添加バルブ
を開き、発酵槽内の無菌空気を添加ラインに導入して行
なうようにしているので、装置構成を簡素化でき操作を
容易化できる。According to the present example, the microorganisms in the inoculum do not contaminate the surrounding outside air or the worker, and can be satisfactorily used in the environment where biohazard measures are taken. Further, the inoculum can be kept completely aseptic during the inoculation operation. Further, since the addition port of the fermenter is provided on the side wall of the upper part of the fermenter and the pot is installed below the side of the fermenter, the operation is easy and the pot can be placed on the floor or the like. Therefore, the capacity is not limited, and a large pot of liquid can be easily attached and detached. On the other hand, since a (sterile) filter is installed in the pot, it is not necessary to newly install a sterile air line. Furthermore, since a steam valve is installed at the addition port, the addition line after addition can be easily sterilized, the addition valve is cooled to open the addition valve after sterilization, and sterile air in the fermentation tank is introduced into the addition line. Since it is performed by using the above-mentioned method, the device configuration can be simplified and the operation can be facilitated.
本考案によれば、培養中の微生物や種菌中の微生物が周
囲の外気あるいは作業者を汚染するのを防止でき、バイ
オハザード対策を行った環境の中で簡潔構造の装置を容
易に使用できるという効果がある。According to the present invention, it is possible to prevent the microorganisms in culture or the microorganisms in the inoculum from contaminating the surrounding outside air or workers, and it is possible to easily use the device with a simple structure in an environment where biohazard measures are taken. effective.
第１図は、本考案の一実施例の添加装置の系統図であ
る。 １……発酵槽、２……ポット、３……空気供給バルブ、
４……蒸気バルブ、 ５……添加バルブ、６……カプラー、 ７，８……殺菌用バルブ、９……バルブ、10……フィル
ター、11……ドレンバルブ、12……蒸気ライン、13……
空気圧送ライン、14,15……ドレンライン、16……添加
口、17……添加ライン、 18……種菌移送ライン、19……空気供給ライン、20……
フタFIG. 1 is a system diagram of an adding device according to an embodiment of the present invention. 1 ... Fermenter, 2 ... Pot, 3 ... Air supply valve,
4 ... Steam valve, 5 ... Addition valve, 6 ... Coupler, 7, 8 ... Sterilization valve, 9 ... Valve, 10 ... Filter, 11 ... Drain valve, 12 ... Steam line, 13 ... …
Pneumatic feed line, 14,15 ... Drain line, 16 ... Addition port, 17 ... Addition line, 18 ... Inoculum transfer line, 19 ... Air supply line, 20 ...
lid
───────────────────────────────────────────────────── フロントページの続き (72)考案者 加藤 健児 山口県下松市大字東豊井794番地 株式会 社日立製作所笠戸工場内 (56)参考文献 特開 昭61−124372（ＪＰ，Ａ) ─────────────────────────────────────────────────── ─── Continuation of the front page (72) Kenji Kato, 794, Higashi-Toyoi, Kudamatsu City, Yamaguchi Prefecture Inside the Kasado Plant, Hitachi Ltd. (56) References JP-A-61-124372 (JP, A)
Claims (1)
加口へ添加バルブ（５），カプラー（６）を介して添加
ラインを接続すると共に、添加バルブ（５）とカプラー
（６）間に蒸気バルブ（４）を有する蒸気ラインを接続
し、前記添加ラインの種菌用バルブ（７）の前段で分岐
させてその一方を種菌用バルブ（８），カプラー（６）
を介してドレンバルブ（１１）を有するドレンラインに
接続し、他方を種菌用バルブ（９）を介して発酵槽側方
の下方に設置したポットに接続される種菌移送ラインへ
接続し、前記ポットに空気圧送ラインを設け、該空気圧
送ラインにフィルター（１０），バルブ（３）を介して
空気ラインを接続し、前記バルブ（４），（７），
（８），（１１）を開いて蒸気ライン，添加ライン，ド
レンラインを殺菌し、ついでバルブ（４）を閉じバルブ
（５）を開いて発酵槽内の無菌空気を供給して添加ライ
ンを冷却し、冷却完了後バルブ（８），（１１）を閉じ
バルブ（９）を開け、次いでバルブ（３）を開けてポッ
ト内に無菌空気を供給するように構成したことを特徴と
する発酵槽の添加物添加装置。1. An addition port of a fermenter is provided on the upper side wall of the tank, and an addition line is connected to the addition port via an addition valve (5) and a coupler (6), and the addition valve (5) and the coupler (6) are connected. ), A steam line having a steam valve (4) is connected between them, and is branched at a stage preceding the inoculum valve (7) of the addition line, and one of them is branched to inoculate valve (8) and coupler (6).
And a drain line having a drain valve (11), and the other through a valve (9) for inoculum to a inoculum transfer line connected to a pot installed below the side of the fermenter. Is provided with an air pressure feed line, the air line is connected to the air pressure feed line via a filter (10) and a valve (3), and the valves (4), (7),
(8) and (11) are opened to sterilize the steam line, the addition line and the drain line, and then the valve (4) is closed and the valve (5) is opened to supply sterile air in the fermenter to cool the addition line. After completion of cooling, the valves (8) and (11) are closed, the valve (9) is opened, and then the valve (3) is opened to supply sterile air into the pot. Additive addition device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1987035902U JPH0616559Y2 (en) | 1987-03-13 | 1987-03-13 | Additive device for fermenter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1987035902U JPH0616559Y2 (en) | 1987-03-13 | 1987-03-13 | Additive device for fermenter |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS63143197U JPS63143197U (en) | 1988-09-21 |
| JPH0616559Y2 true JPH0616559Y2 (en) | 1994-05-02 |
Family
ID=30845816
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1987035902U Expired - Lifetime JPH0616559Y2 (en) | 1987-03-13 | 1987-03-13 | Additive device for fermenter |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0616559Y2 (en) |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS61124372A (en) * | 1984-11-21 | 1986-06-12 | Nippon Roshiyu Kk | Fermenter inoculation device |
-
1987
- 1987-03-13 JP JP1987035902U patent/JPH0616559Y2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPS63143197U (en) | 1988-09-21 |
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