JPH0628740B2 - Sampling method for preparative equipment - Google Patents
Sampling method for preparative equipmentInfo
- Publication number
- JPH0628740B2 JPH0628740B2 JP1207304A JP20730489A JPH0628740B2 JP H0628740 B2 JPH0628740 B2 JP H0628740B2 JP 1207304 A JP1207304 A JP 1207304A JP 20730489 A JP20730489 A JP 20730489A JP H0628740 B2 JPH0628740 B2 JP H0628740B2
- Authority
- JP
- Japan
- Prior art keywords
- piston
- needle
- sample
- layer
- sucking
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Devices For Use In Laboratory Experiments (AREA)
Description
【発明の詳細な説明】 〔発明の利用分野〕 本発明は、注射器のように圧力差で液体を抽出する装置
を有する分取装置の吸い取り機構に関するものである。Description: TECHNICAL FIELD The present invention relates to a suction mechanism of a preparative device having a device for extracting a liquid by a pressure difference like a syringe.
従来、分取装置は第１図に示すように、注射器に相当す
るピストン１とシリンダ２にチップ３を取付け、チップ
３の先端の針６を試験管４内に挿入し、ピストン１をス
テッピングモータ９で上方向に引き上げて、試験管４内
の試料５を底部から吸い取る機構となっており、吸い取
り速度、すなわちピストン１の引き上げ速度は第３図に
示すように一定となっている。Conventionally, as shown in FIG. 1, a sorting apparatus has a tip 3 attached to a piston 1 and a cylinder 2 corresponding to a syringe, a needle 6 at the tip of the tip 3 is inserted into a test tube 4, and the piston 1 is moved to a stepping motor. 9 is a mechanism for pulling the sample 5 in the test tube 4 upward from the bottom, and the suction speed, that is, the pulling speed of the piston 1 is constant as shown in FIG.
しかし、血清を遠心分離した際に試験管底に沈殿するＨ
ＤＬ(High density Lipoprotein)やＨＤＬ＋ＬＤＬ(Low
density Lipoprotein)に代表される粘度の高い沈殿層
１３を吸い取ろうとすると、粘性や慣性により層面が第
３図（Ｅ）のようになり、針６の先端が沈殿層１３の層
面から離れ、他の層（中間層１２）を吸い取ってしまい
第３図（Ｆ）に示すように沈殿層１３の吸い残しを生じ
る場合があった。However, when serum is centrifuged, H that precipitates on the bottom of the test tube
DL (High density Lipoprotein) and HDL + LDL (Low
When attempting to absorb the highly viscous sedimentary layer 13 represented by density lipoprotein), the layer surface becomes as shown in FIG. 3 (E) due to viscosity and inertia, and the tip of the needle 6 separates from the layer surface of the sedimentary layer 13 and In some cases, the layer (intermediate layer 12) was sucked up, and as shown in FIG. 3 (F), the sediment layer 13 was left unsucked.
本発明の目的は、上記した従来技術の欠点をなくし、こ
の種の分取装置の吸い取り精度を向上することである。An object of the present invention is to eliminate the above-mentioned drawbacks of the prior art and improve the suction accuracy of this type of sorting apparatus.
本発明は、密度の異なる液層間で、拡散が無視できる場
合、前記した層間の面が外乱されても、時間とともに重
力方向に層面が再形成される点に着目し、ピストンの移
動する速度を、吸い取り途中において低下させるよう、
ピストン、ステッピングモータの関係を工夫したもので
ある。The present invention focuses on the fact that when diffusion between layers having different densities can be ignored, even if the layers between the layers are disturbed, the layer surface is re-formed in the direction of gravity with time, and the moving speed of the piston , So as to lower it while sucking,
The relationship between the piston and stepping motor has been devised.
血清を遠心分離した後の分離層の吸い取りを例に示し、
本発明の一実施例を第２図に示す。針６の先端が遠心力
により試験管４の底に沈殿した沈殿層１３の中に挿入さ
れた後、第１図に示すピストン１を引き上げシリンダ内
を負圧とすることで沈殿層１３を吸い取る。また、ピス
トン１の移動は第２図のグラフに示すように、途中で一
時動きを停止させる機構となっている。Shown as an example is the absorption of the separation layer after centrifuging the serum,
An embodiment of the present invention is shown in FIG. After the tip of the needle 6 is inserted into the sedimentation layer 13 deposited on the bottom of the test tube 4 by centrifugal force, the piston 1 shown in FIG. 1 is pulled up and the interior of the cylinder is made a negative pressure to suck up the sedimentation layer 13. . Further, the movement of the piston 1 is a mechanism for temporarily stopping the movement on the way as shown in the graph of FIG.
今、ピストン１の移動を第２図のグラフ中の（Ｂ）点で
一時停止させると、分離層の粘性や慣性のために針６先
端まで入り込んだ中間層１２も時間とともに（Ｃ）に示
すように重力方向に戻るのでグラフ中の（Ｃ）点で再度
ピストン１を移動させることにより中間層１２を吸い取
る量を抑え、且つ従来試験管４の底に残った沈殿層１３
を吸うことが可能となる。Now, when the movement of the piston 1 is temporarily stopped at point (B) in the graph of FIG. 2, the intermediate layer 12 that has entered the tip of the needle 6 due to the viscosity and inertia of the separation layer is also shown in (C) with time. Since it returns to the direction of gravity like this, by moving the piston 1 again at point (C) in the graph, the amount of sucking up the intermediate layer 12 is suppressed, and the settling layer 13 remaining at the bottom of the conventional test tube 4 is suppressed.
It becomes possible to smoke.
本発明によれば、針の端部を分取すべき試料中の層に配
置し、所定の速度によりピストンを針から遠ざかる方向
に所定の距離移動させた後、ピストンを移動させる速度
を所定の速度よりも小さくするようにしたので、試験管
内の粘度の高い液を精度で吸い取ることが可能となる。According to the present invention, the end of the needle is arranged in a layer in the sample to be collected, and the piston is moved at a predetermined speed after moving the piston at a predetermined speed in a direction away from the needle. Since the speed is made smaller than the speed, it becomes possible to suck up the highly viscous liquid in the test tube with high accuracy.
第１図に分取装置の吸い取り部の一実施例を示す断面
図、第２図に本発明になる吸い取り機構を説明するグラ
フと吸い取り状況図、第３図に従来の吸い取り機構を説
明するグラフと吸い取り状況図を示す。 図において、１はピストン、２はシリンダ、３はチッ
プ、４は試験管、５は試料、６は針、７はボールネジ、
９はステッピングモータ、１２は中間層、１３は沈殿層
である。FIG. 1 is a cross-sectional view showing an embodiment of a sucking section of a sorting apparatus, FIG. 2 is a graph explaining a sucking mechanism according to the present invention and a sucking state diagram, and FIG. 3 is a graph explaining a conventional sucking mechanism. And shows the sucking situation diagram. In the figure, 1 is a piston, 2 is a cylinder, 3 is a tip, 4 is a test tube, 5 is a sample, 6 is a needle, 7 is a ball screw,
9 is a stepping motor, 12 is an intermediate layer, and 13 is a precipitation layer.
Claims (3)
試料の中に配置される針と、該針の他端部に取り付けら
れた中空のシリンダと、該シリンダ内で摺動可能なピス
トンと、該ピストンを該シリンダ内で該シリンダの軸方
向に移動させるために動力を供給するモータとを有する
分取装置において、前記針の端部を分取すべき試料中の
層に配置し、所定の速度により前記ピストンを前記針か
ら遠ざかる方向に所定の距離移動させた後、前記ピスト
ンを移動させる速度を該所定の速度よりも小さくしたこ
とを特徴とした分取装置の試料吸い取り方法。1. A needle, the end of which is placed in the sample for sucking the sample in a test tube, a hollow cylinder attached to the other end of the needle, and slidable in the cylinder. In a preparative device having a piston and a motor supplying power to move the piston in the cylinder in the axial direction of the cylinder, the end of the needle is arranged in a layer in a sample to be dispensed. A method for sucking a sample in a preparative device, wherein after moving the piston at a predetermined speed in a direction away from the needle by a predetermined distance, the speed at which the piston is moved is made smaller than the predetermined speed.
ら遠ざかる方向に移動させた後、前記ピストンを停止さ
せることを特徴とした請求項１記載の分取装置の試料吸
い取り方法。2. The method for sucking up a sample in a preparative device according to claim 1, wherein the piston is stopped after the piston is moved in a direction away from the needle at a predetermined speed.
中の層は前記試験管の底部に位置することを特徴とした
請求項１記載の分取装置の試料吸い取り方法。3. The method of claim 1, wherein the layer in the sample to be dispensed in which the end of the needle is arranged is located at the bottom of the test tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1207304A JPH0628740B2 (en) | 1989-08-09 | 1989-08-09 | Sampling method for preparative equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1207304A JPH0628740B2 (en) | 1989-08-09 | 1989-08-09 | Sampling method for preparative equipment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0372956A JPH0372956A (en) | 1991-03-28 |
| JPH0628740B2 true JPH0628740B2 (en) | 1994-04-20 |
Family
ID=16537560
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1207304A Expired - Fee Related JPH0628740B2 (en) | 1989-08-09 | 1989-08-09 | Sampling method for preparative equipment |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0628740B2 (en) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5267389A (en) * | 1975-12-01 | 1977-06-03 | Kuraray Co | Method and apparatus for precise and separate taking of liquids of multi components system |
| JPS63205567A (en) * | 1987-02-20 | 1988-08-25 | Nittec Co Ltd | Pipetter for reagent |
-
1989
- 1989-08-09 JP JP1207304A patent/JPH0628740B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0372956A (en) | 1991-03-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |