JPH06510428A - Ｔ−型遺伝子６エキソヌクレアーゼでのｄｎａ配列決定 - Google Patents

Ｔ−型遺伝子６エキソヌクレアーゼでのｄｎａ配列決定

Info

Publication number: JPH06510428A
Authority: JP; Japan
Prior art keywords: dna; strand; primer; exonuclease; sequencing
Prior art date: 1991-08-15
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Pending

Application number

JP5504453A

Other languages

English (en)

Japanese (ja)

Inventor

フラー，カール・ダブリュー

Original Assignee

アマーシャム・ファルマシア・バイオテック・インコーポレーテッド

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1991-08-15

Filing date

1992-08-14

Publication date

1994-11-24

1992-08-14 Application filed by アマーシャム・ファルマシア・バイオテック・インコーポレーテッド filed Critical アマーシャム・ファルマシア・バイオテック・インコーポレーテッド

1994-11-24 Publication of JPH06510428A publication Critical patent/JPH06510428A/ja

Status Pending legal-status Critical Current

Links

108060002716 Exonuclease Proteins 0.000 title claims description 36
102000013165 exonuclease Human genes 0.000 title claims description 36
108090000623 proteins and genes Proteins 0.000 title claims description 23
238000001712 DNA sequencing Methods 0.000 title description 13
108020004414 DNA Proteins 0.000 claims description 136
238000000034 method Methods 0.000 claims description 39
102000053602 DNA Human genes 0.000 claims description 25
102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 7
108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 7
230000000295 complement effect Effects 0.000 claims description 5
102000039446 nucleic acids Human genes 0.000 claims description 5
108020004707 nucleic acids Proteins 0.000 claims description 5
150000007523 nucleic acids Chemical class 0.000 claims description 5
238000010348 incorporation Methods 0.000 claims description 4
102000004190 Enzymes Human genes 0.000 claims description 3
108090000790 Enzymes Proteins 0.000 claims description 3
RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 claims description 3
241001112471 Lambdavirus Species 0.000 claims description 2
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239000003795 chemical substances by application Substances 0.000 claims description 2
239000002299 complementary DNA Substances 0.000 claims description 2
238000003199 nucleic acid amplification method Methods 0.000 claims description 2
238000000338 in vitro Methods 0.000 claims 2
108020004635 Complementary DNA Proteins 0.000 claims 1
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239000007850 fluorescent dye Substances 0.000 claims 1
238000012163 sequencing technique Methods 0.000 description 56
238000006243 chemical reaction Methods 0.000 description 21
239000002585 base Substances 0.000 description 16
238000003752 polymerase chain reaction Methods 0.000 description 15
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108010055863 gene b exonuclease Proteins 0.000 description 14
239000000047 product Substances 0.000 description 14
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 12
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239000002773 nucleotide Substances 0.000 description 10
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108020004682 Single-Stranded DNA Proteins 0.000 description 8
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241000701959 Escherichia virus Lambda Species 0.000 description 5
239000003153 chemical reaction reagent Substances 0.000 description 5
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FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
238000002474 experimental method Methods 0.000 description 4
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239000001226 triphosphate Substances 0.000 description 4
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HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
239000011543 agarose gel Substances 0.000 description 3
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238000004925 denaturation Methods 0.000 description 3
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125000002264 triphosphate group Chemical class [H]OP(=O)(O[H])OP(=O)(O[H])OP(=O)(O[H])O* 0.000 description 3
239000013598 vector Substances 0.000 description 3
ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
108091028043 Nucleic acid sequence Proteins 0.000 description 2
102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 2
108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 2
VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
239000002253 acid Substances 0.000 description 2
230000001580 bacterial effect Effects 0.000 description 2
238000003776 cleavage reaction Methods 0.000 description 2
HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 description 2
238000012869 ethanol precipitation Methods 0.000 description 2
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230000007017 scission Effects 0.000 description 2
239000001632 sodium acetate Substances 0.000 description 2
235000017281 sodium acetate Nutrition 0.000 description 2
239000011780 sodium chloride Substances 0.000 description 2
229910052717 sulfur Inorganic materials 0.000 description 2
239000011593 sulfur Substances 0.000 description 2
238000003786 synthesis reaction Methods 0.000 description 2
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
101150106899 28 gene Proteins 0.000 description 1
GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
229930024421 Adenine Natural products 0.000 description 1
229920000936 Agarose Polymers 0.000 description 1
241000894006 Bacteria Species 0.000 description 1
230000007018 DNA scission Effects 0.000 description 1
230000006820 DNA synthesis Effects 0.000 description 1
108010042407 Endonucleases Proteins 0.000 description 1
102000004533 Endonucleases Human genes 0.000 description 1
241000588724 Escherichia coli Species 0.000 description 1
101100478058 Homo sapiens SP6 gene Proteins 0.000 description 1
102000004317 Lyases Human genes 0.000 description 1
108090000856 Lyases Proteins 0.000 description 1
239000007993 MOPS buffer Substances 0.000 description 1
108010010677 Phosphodiesterase I Proteins 0.000 description 1
102000009609 Pyrophosphatases Human genes 0.000 description 1
108010009413 Pyrophosphatases Proteins 0.000 description 1
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239000007983 Tris buffer Substances 0.000 description 1
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229960000643 adenine Drugs 0.000 description 1
239000003513 alkali Substances 0.000 description 1
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238000000137 annealing Methods 0.000 description 1
QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
239000007853 buffer solution Substances 0.000 description 1
230000015556 catabolic process Effects 0.000 description 1
210000004027 cell Anatomy 0.000 description 1
239000007795 chemical reaction product Substances 0.000 description 1
210000000349 chromosome Anatomy 0.000 description 1
238000010367 cloning Methods 0.000 description 1
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SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 description 1
SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 description 1
RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 description 1
UFJPAQSLHAGEBL-RRKCRQDMSA-N dITP Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(N=CNC2=O)=C2N=C1 UFJPAQSLHAGEBL-RRKCRQDMSA-N 0.000 description 1
238000000354 decomposition reaction Methods 0.000 description 1
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239000005546 dideoxynucleotide Substances 0.000 description 1
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239000011521 glass Substances 0.000 description 1
230000000415 inactivating effect Effects 0.000 description 1
ODBLHEXUDAPZAU-UHFFFAOYSA-N isocitric acid Chemical compound OC(=O)C(O)C(C(O)=O)CC(O)=O ODBLHEXUDAPZAU-UHFFFAOYSA-N 0.000 description 1
229910001629 magnesium chloride Inorganic materials 0.000 description 1
238000004519 manufacturing process Methods 0.000 description 1
238000013507 mapping Methods 0.000 description 1
238000010369 molecular cloning Methods 0.000 description 1
QPUKANZXOGADOB-UHFFFAOYSA-N n-dodecyl-n-methylnitrous amide Chemical class CCCCCCCCCCCCN(C)N=O QPUKANZXOGADOB-UHFFFAOYSA-N 0.000 description 1
230000008520 organization Effects 0.000 description 1
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229920002401 polyacrylamide Polymers 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
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239000012266 salt solution Substances 0.000 description 1
239000011734 sodium Substances 0.000 description 1
238000001179 sorption measurement Methods 0.000 description 1
108010068698 spleen exonuclease Proteins 0.000 description 1
239000012089 stop solution Substances 0.000 description 1
238000006467 substitution reaction Methods 0.000 description 1
ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 1
238000012546 transfer Methods 0.000 description 1
UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
241001515965 unidentified phage Species 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2795/00—Bacteriophages
- C12N2795/00011—Details
- C12N2795/10011—Details dsDNA Bacteriophages
- C12N2795/10311—Siphoviridae
- C12N2795/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/14—Heterocyclic carbon compound [i.e., O, S, N, Se, Te, as only ring hetero atom]
- Y10T436/142222—Hetero-O [e.g., ascorbic acid, etc.]
- Y10T436/143333—Saccharide [e.g., DNA, etc.]

Landscapes

Chemical & Material Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Organic Chemistry (AREA)
Engineering & Computer Science (AREA)
Genetics & Genomics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Biophysics (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Molecular Biology (AREA)
Biochemistry (AREA)
Microbiology (AREA)
Physics & Mathematics (AREA)
Biomedical Technology (AREA)
Immunology (AREA)
Analytical Chemistry (AREA)
Gastroenterology & Hepatology (AREA)
Virology (AREA)
Medicinal Chemistry (AREA)
Crystallography & Structural Chemistry (AREA)
Plant Pathology (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

JP5504453A 1991-08-15 1992-08-14 Ｔ−型遺伝子６エキソヌクレアーゼでのｄｎａ配列決定 Pending JPH06510428A (ja)

Applications Claiming Priority (5)

Application Number	Priority Date	Filing Date	Title
US74519391A	1991-08-15	1991-08-15
US745,193		1991-08-15
US927,562		1992-08-06
US07/927,562 US5516633A (en)	1991-08-15	1992-08-06	DNA sequencing with a T7-type gene 6 exonuclease
PCT/US1992/006826 WO1993004184A1 (fr)	1991-08-15	1992-08-14	Sequencage de l'adn avec une exonuclease d'un gene 6 de type t7

Publications (1)

Publication Number	Publication Date
JPH06510428A true JPH06510428A (ja)	1994-11-24

Family

ID=27114416

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP5504453A Pending JPH06510428A (ja)	1991-08-15	1992-08-14	Ｔ−型遺伝子６エキソヌクレアーゼでのｄｎａ配列決定

Country Status (6)

Country	Link
US (1)	US5516633A (fr)
EP (1)	EP0598832A4 (fr)
JP (1)	JPH06510428A (fr)
AU (1)	AU2474392A (fr)
CA (1)	CA2115674A1 (fr)
WO (1)	WO1993004184A1 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP2015512251A (ja) *	2012-03-22	2015-04-27	エルジーシー・ジェノミクス・リミテッド	ホスホロチオエート基を含むオリゴヌクレオチドを用いる、ポリメラーゼ連鎖反応による検出システム

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5518901A (en) *	1993-04-19	1996-05-21	Murtagh; James J.	Methods for adapting nucleic acid for detection, sequencing, and cloning using exonuclease
US6028190A (en) *	1994-02-01	2000-02-22	The Regents Of The University Of California	Probes labeled with energy transfer coupled dyes
US6291164B1 (en)	1996-11-22	2001-09-18	Invitrogen Corporation	Methods for preventing inhibition of nucleic acid synthesis by pyrophosphate
EP1074633A1 (fr) *	1999-07-05	2001-02-07	LION Bioscience AG	Nucléotides et leur utilisation pour le séquencage de matrices riche en GC
US20040126765A1 (en) *	2002-12-27	2004-07-01	Adams Craig W.	Method and compositions for sequencing nucleic acid molecules
US7264934B2 (en) *	2004-06-10	2007-09-04	Ge Healthcare Bio-Sciences Corp.	Rapid parallel nucleic acid analysis
US8921046B2 (en)	2008-09-19	2014-12-30	Pacific Biosciences Of California, Inc.	Nucleic acid sequence analysis
US8481264B2 (en) *	2008-09-19	2013-07-09	Pacific Biosciences Of California, Inc.	Immobilized nucleic acid complexes for sequence analysis
WO2015120242A2 (fr) *	2014-02-07	2015-08-13	Rangarajan Sampath	Sensibilité améliorée pour la détection moléculaire de microbes dans la circulation sanguine

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5262306A (en) *	1989-09-26	1993-11-16	Robeson David J	Methods for identifying cercosporin-degrading microorganisms
US5366860A (en) *	1989-09-29	1994-11-22	Applied Biosystems, Inc.	Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
US5354656A (en) *	1989-10-02	1994-10-11	Stratagene	Method of DNA sequencing
EP0450060A1 (fr) *	1989-10-26	1991-10-09	Sri International	Sequen age d'adn

1992
- 1992-08-06 US US07/927,562 patent/US5516633A/en not_active Expired - Fee Related
- 1992-08-14 AU AU24743/92A patent/AU2474392A/en not_active Abandoned
- 1992-08-14 EP EP92918346A patent/EP0598832A4/en not_active Withdrawn
- 1992-08-14 WO PCT/US1992/006826 patent/WO1993004184A1/fr not_active Ceased
- 1992-08-14 JP JP5504453A patent/JPH06510428A/ja active Pending
- 1992-08-14 CA CA002115674A patent/CA2115674A1/fr not_active Abandoned

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
JP2015512251A (ja) *	2012-03-22	2015-04-27	エルジーシー・ジェノミクス・リミテッド	ホスホロチオエート基を含むオリゴヌクレオチドを用いる、ポリメラーゼ連鎖反応による検出システム

Also Published As

Publication number	Publication date
US5516633A (en)	1996-05-14
CA2115674A1 (fr)	1993-03-04
EP0598832A4 (en)	1995-10-25
EP0598832A1 (fr)	1994-06-01
WO1993004184A1 (fr)	1993-03-04
AU2474392A (en)	1993-03-16

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EP0679190B1 (fr)	2003-05-02	Procede pour produire des molecules d'acides nucleiques monocatenaires
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US5646019A (en)	1997-07-08	Method for producing primed nucleic acid templates
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WO1990010064A1 (fr)	1990-09-07	Procedes ameliores pour l'amplification d'adn in vitro et topographie et clonage genomiques
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JPH06510428A - Ｔ−型遺伝子６エキソヌクレアーゼでのｄｎａ配列決定 - Google Patents

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