JPH0657200U - Sterile saline solution in a bag - Google Patents

Abstract

(57) [Summary] [Purpose] A 10-fold dilution can be prepared quickly and easily.
It can be easily sealed with a heat sealer, can be used as it is for the work after making a 10-fold diluted solution, can be immediately refrigerated and frozen, and can stand up in a concentrated and large amount at the time of bacteria inspection. Provide a sterile physiological saline solution in a bag. [Structure] A sterilized saline solution in which a proper amount of physiological saline solution is sealed in an upright, transparent thermoplastic synthetic resin bag for retort sterilization, and retort sterilized.

Description

[Detailed description of the device]

[0001]

[Industrial applications]

 The present invention relates to a sterile physiological saline solution in a bag used for preparing a 10-fold diluted solution in a bacteria test.

[0002]

[Prior art]

 Conventionally, the method for preparing a 10-fold diluted solution used for bacterial tests is to put a required amount of a sample in a sterilized plastic bag or a container for a sterilized homogenizer, and sterilize physiological saline from another container made of a material such as glass. Then, shake with a stomacher or crush with a homogenizer to secure a 10-fold diluted solution, or put 90 ml or 225 ml of physiological saline in a heat-resistant container with a lid and sterilize. However, there is a method in which the sample is aseptically weighed so that dirt and other bacteria do not enter, put in the above heat-resistant container, and shaken to prepare a 10-fold diluted solution. It was being done.

However, in the first half method of the above-mentioned conventional technique, in addition to the container for holding the sample, another container for sterilizing by adding physiological saline was required. Also, in the latter half of the above conventional technique, a glass container or the like is used when the heat-resistant container containing the sample and physiological saline is shaken to crush and stir the sample. In some cases, the container could not be crushed from the outside by deforming the entire container, and the condition of crushing was poor.

From the above, there is a drawback that it takes a lot of labor such as container transfer and cleaning, and many containers are required. In addition, if the sample was not well crushed and a uniform diluted solution could not be obtained, there was a risk that the subsequent test results would be inaccurate. Furthermore, when using a container such as a glass, great care must be taken in handling, and there are drawbacks such as being bulky during storage and not being easily disposable.

[0005]

The present invention has been conceived in order to eliminate the drawbacks of the above-mentioned conventional techniques, and it is possible to prepare a 10-fold diluted solution quickly and easily, and to easily prepare a heat sealer. It can be sealed with, can be used as it is for the work after making a 10-fold diluted solution, and can be immediately refrigerated or frozen, and can be sterilized in a bag that can be inspected intensively and in large quantities during bacterial inspection. It provides physiological saline.

[0006]

[Means for Solving the Problems]

 An embodiment of the present invention will be described with reference to the drawings. FIG. 1 is a perspective view of the sterilized saline solution 1 in a bag of the present invention which is erected from a slightly front side, and is transparent, heat-resistant, durable and soft for retort sterilization. 90 ml or 225 ml of physiological saline 3 is placed in a bag 2 of thermoplastic synthetic resin which also has a rigidity capable of standing up, and the bag is sealed with a heat sealer. The sealed sterile saline solution 1 is subjected to retort sterilization at a temperature of 121 ° C. for 20 minutes or longer, and is configured so that it can stand up with the saline solution inside. . FIG. 2 is a perspective view of the sterilized physiological saline solution 1 in a bag of the present invention, which is erected and which is seen from the side slightly above.

The mechanism for erecting the bag 2 has a structure in which the bottom portion 4 is raised to the bottom to provide stability, but the structure is not limited to this, and the same material is not bulky and stable. Any mechanism that allows the bag to stand up will do.

The physiological saline 3 may be one in which the pH is adjusted with a phosphate buffer or the like depending on the test sample.

Regarding the material of the bag 2, the bag 2 has heat resistance that does not melt or deform during high temperature sterilization at about 120 degrees Celsius, and when it is deformed by being pushed from the outside, Although it is deformed to the extent that it helps crush and stir, it is strong enough not to easily break, is transparent so that the contents can be seen easily, can be sealed with a heat sealer, and does not melt in water or saline. It is a plastic synthetic resin.

If the amount of the physiological saline 3 is 90 ml and the amount of the sample used is 10 g and 225 g, it is possible to prepare a 10-fold diluted solution with 25 g of the sample used. It is advisable to determine the amount of saline solution so that it can be easily calculated, and to determine in advance the amount of several types required for testing.

The sterilized bag-containing sterilized physiological saline, which is filled with physiological saline and hermetically sealed, is stored at room temperature or refrigerated.

When it is necessary to prepare a 10-fold diluted solution, the sterilized physiological saline solution 1 in a bag that has been stored is taken out, stands up, and an alcohol disinfectant solution is sprayed on the portion to be opened. Wipe off with gauze or cotton that has been fully added, and open with sterilized scissors or a scalpel.

Subsequently, in the case of bag-containing sterile physiological saline solution 1 containing 90 ml of physiological saline solution, 10 g of a sample is aseptically collected and opened in the bag-containing sterile physiological saline solution 1. And seal using a heat sealer. Here, in the case of sterile physiological saline in a bag containing 225 ml of physiological saline, collect and use 25 g of the sample.

Furthermore, the contents of the sterilized physiological saline solution in a sealed bag containing a sample are crushed with a stomacher or the like and stirred to prepare a uniform 10-fold diluted solution.

When it is desired to store a 10-fold diluted solution, it can be immediately stored in a refrigerator or frozen at 5 ° C. or less.

When carrying out a bacterial test using a 10-fold diluted solution, the cut portion may be sterilized with an alcohol disinfectant, collected with a sterilized pipette, and various inspection operations may be performed.

When preparing or using a 10-fold diluted solution, except when it is stored, it is possible to always work with the bag 2 standing up, and it is convenient without using other containers. Good workability.

By preparing and storing a large amount of a bag in which a 10-fold diluted solution is heat-sealed, it is possible to intensively test a large amount at a time during the test.

If sterile physiological saline having an unadjusted pH is used, physical properties such as pH and salt content can be measured as necessary after the completion of the bacterial test. Sterile physiological saline with unadjusted pH has a salt concentration of 0.9 percent in advance, so when the salt content is to be determined, the measurement can be performed by subtracting the 0.9 percent concentration.

After the bacteria test and the measurement of physical property values are completed, the remaining material is expensive and is not dangerous if broken, so it can be easily thrown away and sealed. It is not bulky if you keep it, so you can store it as it is.

[0021]

[Effect of device]

 According to this invention, an appropriate amount of sterilized physiological saline necessary for preparing a 10-fold diluted solution is put in a bag and sealed in advance, so that it is possible to work while standing upright. Can be prepared quickly and easily. In addition, since it can be easily sealed with a heat sealer, it can be easily stored and stored, and if a large amount is stored, it is possible to intensively test a large amount at a time during inspection. You can work while the bag is standing up and you can use the bag as it is, which is convenient and easy to work with. Since the bag is flexible, hard to tear and transparent, the contents can be seen easily, the contents can be easily crushed and agitated, it is not bulky when stored, and it is easy to throw it away. Play.

[Brief description of drawings]

FIG. 1 is a perspective view of an embodiment of a sterile physiological saline solution in a bag according to the present invention, which is in a standing state and which is seen from slightly above the front surface.

FIG. 2 is a perspective view of an embodiment of the sterilized saline solution in a bag of the present invention, which is in a standing state and which is viewed from a side slightly above.

[Explanation of symbols]

 1 bag of sterilized saline 2 bags 3 saline 4 Bottom part

Claims (1)

[Scope of utility model registration request] 1. A sterilized saline solution in a bag, which is obtained by sealing an appropriate amount of physiological saline solution in a bag of an upright and transparent thermoplastic synthetic resin for retort sterilization and performing retort sterilization.