JPH07145192A - タンパク質純性化方法及びその純性化確認方法 - Google Patents

タンパク質純性化方法及びその純性化確認方法

Info

Publication number
JPH07145192A
JPH07145192A JP5251960A JP25196093A JPH07145192A JP H07145192 A JPH07145192 A JP H07145192A JP 5251960 A JP5251960 A JP 5251960A JP 25196093 A JP25196093 A JP 25196093A JP H07145192 A JPH07145192 A JP H07145192A
Authority
JP
Japan
Prior art keywords
protein
silica gel
useful
gel sorbent
eluent
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP5251960A
Other languages
English (en)
Japanese (ja)
Inventor
T Rusk Lance
ティー ラスク ランス
Goldstein Henry
ゴールドステイン ヘンリー
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Miller Brewing Co
Original Assignee
Miller Brewing Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US07/882,793 priority Critical patent/US5278284A/en
Priority to ES93115953T priority patent/ES2105033T3/es
Priority to DK93115953.7T priority patent/DK0646594T3/da
Priority to AT93115953T priority patent/ATE154033T1/de
Priority to EP93115953A priority patent/EP0646594B1/de
Priority to DE69311337T priority patent/DE69311337D1/de
Application filed by Miller Brewing Co filed Critical Miller Brewing Co
Priority to JP5251960A priority patent/JPH07145192A/ja
Publication of JPH07145192A publication Critical patent/JPH07145192A/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/20—Partition-, reverse-phase or hydrophobic interaction chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/461—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from fish
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Toxicology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Endocrinology (AREA)
  • General Engineering & Computer Science (AREA)
  • Diabetes (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP5251960A 1992-05-14 1993-10-07 タンパク質純性化方法及びその純性化確認方法 Pending JPH07145192A (ja)

Priority Applications (7)

Application Number Priority Date Filing Date Title
US07/882,793 US5278284A (en) 1992-05-14 1992-05-14 Protein purification method
ES93115953T ES2105033T3 (es) 1992-05-14 1993-10-02 Metodo de purificacion de proteinas.
DK93115953.7T DK0646594T3 (da) 1992-05-14 1993-10-02 Fremgangsmåde til rensning af proteiner
AT93115953T ATE154033T1 (de) 1992-05-14 1993-10-02 Verfahren zur reinigung von proteinen
EP93115953A EP0646594B1 (de) 1992-05-14 1993-10-02 Verfahren zur Reinigung von Proteinen
DE69311337T DE69311337D1 (de) 1992-05-14 1993-10-02 Verfahren zur Reinigung von Proteinen
JP5251960A JPH07145192A (ja) 1992-05-14 1993-10-07 タンパク質純性化方法及びその純性化確認方法

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US07/882,793 US5278284A (en) 1992-05-14 1992-05-14 Protein purification method
EP93115953A EP0646594B1 (de) 1992-05-14 1993-10-02 Verfahren zur Reinigung von Proteinen
JP5251960A JPH07145192A (ja) 1992-05-14 1993-10-07 タンパク質純性化方法及びその純性化確認方法

Publications (1)

Publication Number Publication Date
JPH07145192A true JPH07145192A (ja) 1995-06-06

Family

ID=27235218

Family Applications (1)

Application Number Title Priority Date Filing Date
JP5251960A Pending JPH07145192A (ja) 1992-05-14 1993-10-07 タンパク質純性化方法及びその純性化確認方法

Country Status (7)

Country Link
US (1) US5278284A (de)
EP (1) EP0646594B1 (de)
JP (1) JPH07145192A (de)
AT (1) ATE154033T1 (de)
DE (1) DE69311337D1 (de)
DK (1) DK0646594T3 (de)
ES (1) ES2105033T3 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5451660A (en) * 1993-12-13 1995-09-19 Genentech, Inc. Method for purifying polypeptides
WO1996014571A1 (en) * 1994-11-08 1996-05-17 The Regents Of The University Of California Temperature-gradient osmometry
CA2167872C (en) * 1995-01-27 2007-04-03 Masaru Nakatani Adsorbent for removing interleukins and tumor necrosis factor, and process for removing the same
US5869604A (en) * 1995-11-09 1999-02-09 Georgia Institute Of Technology Crystallization and purification of polypeptides
US6764690B2 (en) * 1996-05-29 2004-07-20 Delsitech Oy Dissolvable oxides for biological applications
AU763349B2 (en) * 1998-02-05 2003-07-17 Henry M. Jackson Foundation For The Advancement Of Military Medicine Simplified method for removing free protein during preparation of protein-polysaccharide conjugates and vaccines using restricted-access media
US6132625A (en) * 1998-05-28 2000-10-17 E. I. Du Pont De Nemours And Company Method for treatment of aqueous streams comprising biosolids
US6203711B1 (en) 1999-05-21 2001-03-20 E. I. Du Pont De Nemours And Company Method for treatment of substantially aqueous fluids derived from processing inorganic materials
US20020173024A1 (en) * 2000-06-08 2002-11-21 Horwath Kathleen L. Nucleic acid sequences encoding type III tenebrio antifreeze proteins and method for assaying activity
US6780330B2 (en) 2001-03-09 2004-08-24 E. I. Du Pont De Nemours And Company Removal of biomaterials from aqueous streams
US20030026845A1 (en) * 2001-06-18 2003-02-06 Etzel Lisa R. Process for preparing protein isolate from milk, whey, colostrum, and the like
ES2322945T5 (es) * 2001-12-21 2012-10-17 Immunex Corporation Métodos para purficación de proteína
US7285269B2 (en) * 2002-12-02 2007-10-23 Amgen Fremont, Inc. Antibodies directed to tumor necrosis factor
US8409647B2 (en) * 2008-08-12 2013-04-02 E. I. Du Pont De Nemours And Company Silica microgels for reducing chill haze
WO2012104339A1 (en) 2011-02-01 2012-08-09 Novo Nordisk A/S Purification of insulin
US10093674B2 (en) 2011-05-31 2018-10-09 Brandeis University Supramolecular nanofibers and hydrogels based on nucleic acids functionalized with nucleobases
WO2012166705A2 (en) * 2011-05-31 2012-12-06 Brandeis University Supramolecular nanofibers and hydrogels based on amino acid-nucleobase-glycoside conjugates
GB201411943D0 (en) 2014-07-03 2014-08-20 Univ Heriot Watt Process and protein product

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2769747A (en) * 1953-02-18 1956-11-06 Armour & Co Process of separation of siliceous impurities from insulin solutions
DE1442334B2 (de) * 1963-08-02 1980-04-24 Wilz-Pauls Geb. Pauls, Rosemarie, Basel (Schweiz) Verfahren zur Behandlung von Bier
GB1215928A (en) * 1967-04-28 1970-12-16 Unilever Ltd A process for reducing haze in beer
US3945988A (en) * 1974-07-16 1976-03-23 Pushpa Khann Process for isolation of insulin from plant source
GB1592702A (en) * 1976-10-28 1981-07-08 Asahi Chemical Ind Method of adsorbing a protein on a protein adsorbent comprising a porous copolymer of a cyano-group-containing monomer
JPS5810056A (ja) * 1981-07-10 1983-01-20 株式会社クラレ 血液浄化装置
DE3147842A1 (de) * 1981-12-03 1983-06-23 Hoechst Ag, 6230 Frankfurt "verfahren zur trennung von gemischen aus insulin, insulinderivaten und gegebenenfalls verunreinigungen"
CA1178222A (en) * 1982-02-04 1984-11-20 Chang W. Chi Composition and method for stabilizing beverages against haze formation
HU190129B (en) * 1983-07-11 1986-08-28 Reanal Finomvegyszergyar,Hu Process for the isolation of carboxypeptidase b enzyme from mammal pancreas
US4528198A (en) * 1983-09-27 1985-07-09 Miller Brewing Company Preparation of low calorie beer with malt extract free of yeast lethal factors
US4636394A (en) * 1984-04-16 1987-01-13 J. E. Siebel Sons' Company, Inc. Method and compositions for chillproofing beverages
US4595578A (en) * 1985-03-22 1986-06-17 Scm Corporation Stabilized silica gel and process for making same
US4684530A (en) * 1985-10-04 1987-08-04 W. R. Grace & Co. Adsorption of proteins from fluids
JPS62207712A (ja) * 1986-03-05 1987-09-12 Fuji Debuison Kagaku Kk ビ−ルの安定化処理用含水シリカゲル
DE3633797C2 (de) * 1986-10-03 1995-08-10 Forssmann Wolf Georg Verfahren zur Gewinnung von biologisch aktiven Eiweissen (Peptiden) aus menschlichem und tierischem Blut (bzw. anderen Körperflüssigkeiten)
GB8707526D0 (en) * 1987-03-30 1987-05-07 Unilever Plc Amorphous silicas
JP2732252B2 (ja) * 1987-12-07 1998-03-25 株式会社ジェイ・エム・エス ヘモグロビン選択吸着剤
US4929560A (en) * 1988-02-03 1990-05-29 Damon Biotech, Inc. Recovery of tissue plasminogen activator
US5086167A (en) * 1988-11-28 1992-02-04 University Of Miami Method of separating proteins

Also Published As

Publication number Publication date
ATE154033T1 (de) 1997-06-15
DE69311337D1 (de) 1997-07-10
DK0646594T3 (da) 1997-12-22
EP0646594A1 (de) 1995-04-05
ES2105033T3 (es) 1997-10-16
US5278284A (en) 1994-01-11
EP0646594B1 (de) 1997-06-04

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