JPH07187A - Classification of substance extracted from rice and rice bran - Google Patents
Classification of substance extracted from rice and rice branInfo
- Publication number
- JPH07187A JPH07187A JP10867793A JP10867793A JPH07187A JP H07187 A JPH07187 A JP H07187A JP 10867793 A JP10867793 A JP 10867793A JP 10867793 A JP10867793 A JP 10867793A JP H07187 A JPH07187 A JP H07187A
- Authority
- JP
- Japan
- Prior art keywords
- supernatant
- rice
- minutes
- rice bran
- confirmed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000209094 Oryza Species 0.000 title claims abstract description 38
- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 38
- 235000009566 rice Nutrition 0.000 title claims abstract description 38
- 239000000126 substance Substances 0.000 title claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000006228 supernatant Substances 0.000 claims abstract description 19
- 239000012153 distilled water Substances 0.000 claims abstract description 14
- 230000008014 freezing Effects 0.000 claims abstract description 3
- 238000007710 freezing Methods 0.000 claims abstract description 3
- 238000000034 method Methods 0.000 claims description 13
- 229920001817 Agar Polymers 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 239000013049 sediment Substances 0.000 claims description 4
- 239000013618 particulate matter Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 241000409991 Mythimna separata Species 0.000 claims description 2
- 239000011148 porous material Substances 0.000 claims description 2
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims 2
- 229910052782 aluminium Inorganic materials 0.000 claims 2
- 239000011888 foil Substances 0.000 claims 2
- 244000061456 Solanum tuberosum Species 0.000 claims 1
- 235000002595 Solanum tuberosum Nutrition 0.000 claims 1
- 238000007865 diluting Methods 0.000 claims 1
- 230000004927 fusion Effects 0.000 claims 1
- 230000020169 heat generation Effects 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 238000010257 thawing Methods 0.000 claims 1
- 238000010438 heat treatment Methods 0.000 abstract description 4
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- 238000010792 warming Methods 0.000 abstract 1
- 239000002609 medium Substances 0.000 description 5
- 239000001965 potato dextrose agar Substances 0.000 description 4
- 150000001413 amino acids Chemical class 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 2
- 235000017491 Bambusa tulda Nutrition 0.000 description 2
- 241001330002 Bambuseae Species 0.000 description 2
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 2
- 239000011425 bamboo Substances 0.000 description 2
- 238000012512 characterization method Methods 0.000 description 2
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 2
- 229960005091 chloramphenicol Drugs 0.000 description 2
- 235000021186 dishes Nutrition 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 239000011345 viscous material Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000012742 biochemical analysis Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- MHDVGSVTJDSBDK-UHFFFAOYSA-N dibenzyl ether Chemical compound C=1C=CC=CC=1COCC1=CC=CC=C1 MHDVGSVTJDSBDK-UHFFFAOYSA-N 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000003801 milling Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は人々が見落としているも
のを比較的簡単な方法で解決したものである。 イ）米、米糠を用いる。 ロ）イ）を蒸留水に溶解する。 ハ）ロ）を撹拌して各々の条件に合わせ、その上清を使
用する。 ニ）１０％ポテトデキストローズ寒天（ＰＤＡ）培地を
用いる。（含クロラムフェニコール２５ｍｇ／Ｌ） ホ）３７゜ｃ．７４°ｃのインキュベーターに静置 この発明は米、米糠に存在するものを凍結、冷蔵庫、室
温、熱処理、加熱等々で処理することによって注目する
行動パターンをとる物質に着眼した結果である。なお、
説明を加えるうえで、これらの物質（ＴＫ−１，ＴＫ−
２，ＴＲ−１，ＴＲ−２等）の総称としてＳＥＮＫＡ
（センカ）の記号を用いる。FIELD OF THE INVENTION The present invention solves what people overlook in a relatively simple manner. B) Use rice and rice bran. B) Dissolve b) in distilled water. C) Stir (b) and adjust to each condition, and use the supernatant. D) Use 10% potato dextrose agar (PDA) medium. (Chloramphenicol-containing 25 mg / L) e) 37 ° c. The present invention is the result of focusing on substances that have an action pattern of interest by treating rice and rice bran that are present in freezing, refrigerator, room temperature, heat treatment, heating, etc. In addition,
In adding explanation, these substances (TK-1, TK-
2, TR-1, TR-2, etc.)
The symbol (senka) is used.
【０００２】[0002]
【従来の技術】米、米糠は日本人の主食の米を精白する
ときに生ずる米の皮が粉になったものであり、人に有害
とされるものは含有していない。こうした米糠から得ら
れる糠は直接、間接病的状態を起こさせるものは先ずな
いといって良い。この米糠に至ってはビタミン、アミノ
酸等々を含んんでいて栄養価としては高いものがある。
例えば、脂肪、窒素、ビタミン、 アミノ酸、有機酸、
無機質、糖質等が見知されている。このようなものに対
して全くといって良いほど技術及び研究の開発がなされ
ていなかった。2. Description of the Related Art Rice and rice bran are produced by milling rice, which is a staple food of Japanese people, and the skin of the rice is powdered, and does not contain anything harmful to humans. It is safe to say that the rice bran obtained from such rice bran is unlikely to cause direct or indirect pathological conditions. This rice bran contains vitamins, amino acids, etc. and has a high nutritional value.
For example, fat, nitrogen, vitamins, amino acids, organic acids,
Inorganic substances and sugars are known. Technology and research have not been developed to such an extent.
【０００３】[0003]
【発明が解決しようとする課題】従ってこの米、米糠に
含まれている物質の抽出から始めた米糠を蒸留水に溶解
させ、凍結、熱処理、煮沸等々を試み、これら条件によ
って抽出された物質を解折することにある。Therefore, the rice bran, which was started by extracting the substances contained in the rice and rice bran, is dissolved in distilled water and tried to be frozen, heat-treated, boiled, etc., and the substances extracted under these conditions are It's about breaking up.
【０００４】[0004]
本発明は前記した様に比較的簡単な操作方法で抽出した
物質（ＳＥＮＫＡ）の性格づけである、生物化学成分の
検討に入るため種々の実験操作方方をとつた。 超音波処理（型式 ＴＷＥ０５０ ウメダ科学） 遠心機（型式 Ｈ−５０１ 国産遠心機）他、酵素処
理、酸・アルカリ処理、ガスクロマトグラフィー、液化
クロマトグラフィー、ジェチルエーテル抽出法、ゲルダ
ール法、アミノ酸分析等々を行った。As described above, the present invention employs various experimental operation methods in order to enter into a study of biochemical components, which is the characterization of a substance (SENKA) extracted by a relatively simple operation method. Ultrasonic treatment (type TWE050 Umeda Science) Centrifuge (type H-501 domestic centrifuge), enzyme treatment, acid / alkali treatment, gas chromatography, liquefaction chromatography, benzyl ether extraction method, Gerdal method, amino acid analysis, etc. I went.
【０００５】[0005]
【作用と実施例】次に本発明の実施方法とその実験結果
を例にあげて説明する。Next, the method of carrying out the present invention and the experimental results thereof will be described as an example.
【０００６】第一例（寒天培地） 米糠１０ｇを計量し、滅菌水（蒸留水をオートクレーブ
にかける）９０ｍｌに溶かし、よく撹拌後に静置（室
温）上清１０ｍｌを滅菌水にする。よく懸濁後１ｍｌを
とりだし９０ｍｍΦシヤーレ内の１０％ＰＤＡ培地（ポ
テトデキストローズ寒天培地、含クロラムフェニコール
２５ｍｇ／Ｌ）に撤く、シャーレ１０枚用意、３７℃
と７４℃の恒温器（型式ＩＳ４２、ヤマト科学）に各５
枚づつ設置 （１）３７℃インキュベーター ３日以降集落（コロニー）をつくりはじめる。７〜１０
日以降集落が径２〜３ｍｍ位となり肉眼で認められる。
この時期新しい寒天培地に植換てもよい。但、そのまま
の状態であると３週間以降は隣接境界が分からなくな
る。なお、植換の方法は「竹ぐし」 でもよい
がコロニーが大きくなると滅菌水に溶かして撤いても形
態的に異なることはない。 形状及び色 イ）乳白色（粘性様） ロ）白色系 球形 ハ）赤色系 〃 ニ）黄色系 中心部がノコギリ状 竹ぐしで取り、試験管に状況によって１ｍｌの滅菌水に
溶かし、よく懸濁して全量を寒天上に撤く、コロニーの
サイズによって竹ぐしだけで継代もする。 （２）７４℃インキュベーター 集落は１ｍｍ前後（撒いて４日目）で増殖はなし継代し
ても２〜３代で消滅。但、同形と認められる継代も可能
である。高温では量が問題となる１０日目以降になると
１ｍｍ前後のコロニーの集合体の中心寄り当たりからド
ーム状型に隆起した形状が生じる空洞もあるが密集もし
ている。First Example (Agar Medium) 10 g of rice bran is weighed and dissolved in 90 ml of sterilized water (distilled water is put in an autoclave), and after stirring well, 10 ml of the supernatant (room temperature) is made into sterilized water. After suspending well, take out 1 ml and remove to 10% PDA medium (potato dextrose agar medium, chloramphenicol-containing 25 mg / L) in a 90 mmΦ dish, prepare 10 petri dishes, 37 ° C.
And 5 each for 74 ° C incubator (model IS42, Yamato Scientific)
Install one by one (1) Incubator at 37 ℃ Start colonization after 3 days. 7-10
After the day, the settlement became 2-3 mm in diameter and visible to the naked eye.
At this time, the agar medium may be replaced with new one. However, if it is left as it is, the adjoining boundary will be unknown after 3 weeks. The method of replanting may be “bamboo gush”, but when the colony grows large, it does not differ morphologically even if it is dissolved in sterile water and removed. Shape and color a) Milky white (viscous-like) b) White type spherical c) Red type d) Yellow type The center part is saw-shaped Takeshi picked up, dissolved in 1 ml of sterilized water and suspended well in a test tube. Remove the whole amount on agar, and pass it with only bamboo paste depending on the size of the colony. (2) 74 ° C. incubator The colony did not grow at around 1 mm (4 days after seeding) and disappeared in 2 to 3 generations even after passage. However, passages that are recognized as isomorphic are possible. After the 10th day, when the amount becomes a problem at high temperatures, there are cavities that form a dome-shaped bulge from the center of the aggregate of colonies around 1 mm, but they are also dense.
【０００７】第２例（蒸留水） 第一例で行ったものと同方法で操作した。 静置後 上清を滅菌シャーレ（９０ｍｍΦ）６枚に分注（１５ｍ
ｌ／ＬＰ）後、それぞれの恒温器に設置 ・恒温器７４℃ ２枚 ・ 〃 ３７℃ ２枚 ・室 温 ２枚 毎日観察の結果ＳＥＮＫＡはいずれの状態においてもみ
とめられたが増殖率においては高温ほど高い。顕微鏡
（型式ＩＭＴ−２、オリンパス光学社）下で確認。４日
前後が良好な状態である。継代においては残存する量の
１／２〜１／３で１週間毎が良い。また、大量培養に適
する温度は５０℃以上が良好である。ＴＫ−１において
は高温ほど形状は小さくなるが、独自の行動パターンを
とることにおいては同様である。ＴＫ−２及びＴＲ−１
の形状においては高温（７４℃）、低温（３７℃）でも
差異は認められないが、行動パターンにおいては、ＴＫ
−２は認められるがＴＲ−１では認められない。また、
共に光沢があるが、ややＴＲ−１のほうが大きい。ＴＲ
−２においては形状に特色が認められる。寒天上では視
覚出来ない。ＴＲ−２が大きく変化すると同時に又、そ
の成長過程での予備群というのが認められた。Second Example (Distilled Water) An operation was carried out in the same manner as in the first example. After standing, dispense the supernatant into 6 sterile petri dishes (90 mmΦ) (15 m
(1 / LP), and then installed in each incubator ・ Incubator 74 ℃ 2 sheets ・ 〃 37 ℃ 2 sheets ・ Room temperature 2 sheets As a result of daily observation, SENKA was found in any state, but the growth rate was high. Moderately expensive. Confirmed under a microscope (model IMT-2, Olympus Optical Co.). Around 4 days is in good condition. In the passage, 1/2 to 1/3 of the remaining amount is preferable every week. Further, a temperature suitable for large-scale culture is preferably 50 ° C. or higher. In TK-1, the higher the temperature, the smaller the shape, but the same is true in taking a unique behavior pattern. TK-2 and TR-1
There is no difference in the shape of the animals at high temperature (74 ℃) and low temperature (37 ℃), but in the behavior pattern, TK
-2 is observed but not TR-1. Also,
Both are glossy, but TR-1 is slightly larger. TR
At -2, a characteristic is recognized in the shape. Not visible on agar. At the same time that TR-2 changed greatly, it was also recognized that it was a preliminary group in the course of its growth.
【０００８】第３例（ＳＥＮＫＡの大量培養） 米、米糠５００ｇを４．５ｌの蒸留水に溶解させ撹拌後
５０℃のインキュベーターに設置３日目に上清を遠心機
にて分離する。遠心機（型式Ｈ−５０１、国産遠心機）
を用い４０００ｒ．ｐ．ｍ．１０分、室温にて上清と沈
渣物を分ける。上清にはＴＲ系（ＴＲ−１、ＴＲ−２）
が集まり、沈渣物には（ＴＫ−１、ＴＫ−２）が集合す
る。ＴＲ系をさらに８０００ｒ．ｐ．ｍ．２０分（室
温）にても沈降せずメブランフィルターを用いる。ミリ
ポアフィルター、ポアサイズ０．４５μを用いて、ゆっ
くり加圧（人力）フィルター上に吸着させ１５ｍｌ温水
にて解離した。３０分静置漸次加温、顕微鏡下で確認し
成分を検討中である。（第二報）Third Example (mass culture of SENKA) 500 g of rice and rice bran are dissolved in 4.5 l of distilled water, stirred and placed in an incubator at 50 ° C., and the supernatant is separated by a centrifuge on the third day. Centrifuge (Model H-501, domestic centrifuge)
4000r. p. m. Separate supernatant and sediment for 10 minutes at room temperature. TR system (TR-1, TR-2) in the supernatant
Are collected and (TK-1, TK-2) are collected in the sediment. TR system is further 8000 r. p. m. Even after 20 minutes (room temperature), sedimentation does not occur, and a membrane filter is used. Using a Millipore filter and a pore size of 0.45μ, the mixture was slowly adsorbed on a pressure (manual) filter and dissociated with 15 ml of warm water. It is still standing for 30 minutes, gradually heated, and confirmed under a microscope to examine the components. (Second report)
【０００９】[0009]
【発明の効果】以上の例証及び請求項の性格づけをする
と、米糠に含まれるＳＥＮＫＡは５０℃の恒温の場合に
は大量培養に適している。７０℃以上の場合にはＴＲ−
１、ＴＲ−２が特色を持つ、１２１℃、１．６気圧、３
０分において及び１２３℃、１．６気圧、３０分では、
ＳＥＮＫＡは、それぞれの行動パターンが認められる。
１５８℃、４．９気圧、３０分ではＴＫ−２は見知出来
なかったが、３日目当たりから確認出来た。サンプルは
（７４℃に設置）日数を置いて増加するのが認められ
た。２００℃、２０分ともに炭化状態であったがＴＫ−
１が確認出来たが、現在顕微鏡下にある。生物化学分析
も現在進行中である（第二報）又、３７℃で現れてき
た、赤色糸、白色系、黄色系、乳白色系では、赤色系、
白色系は高温での増加は見られない。黄色系は進行中で
ある。高温では乳白色が増殖可能であることが認められ
た。EFFECTS OF THE INVENTION From the above characterization and characterizing the claims, SENKA contained in rice bran is suitable for mass culture at a constant temperature of 50 ° C. TR-in case of 70 ℃ or higher
1, TR-2 has a characteristic, 121 ℃, 1.6 atmospheric pressure, 3
At 0 minutes and at 123 ° C., 1.6 atmospheres, 30 minutes,
In SENKA, each behavior pattern is recognized.
TK-2 could not be detected at 158 ° C, 4.9 atm, and 30 minutes, but it was confirmed from around the third day. The sample was found to increase over time (installed at 74 ° C). It was carbonized at 200 ° C for 20 minutes, but TK-
1 was confirmed, but it is now under the microscope. Biochemical analysis is also in progress (2nd report). Also, red thread, white, yellow, and milky white that appeared at 37 ° C are red,
The white system does not show any increase at high temperature. Yellow system is in progress. It was found that milky white could grow at high temperature.
（生物の形態の範囲である。） (This is the range of forms of living things.)
【図 １】米糠（米も同様）を蒸留水に溶かした時に、
認められる黒色顆粒状物質（ＴＫ−１）
（６６０倍）[Figure 1] When rice bran (also rice) was dissolved in distilled water,
Black granular material (TK-1) found
(660 times)
【図 ２】黒色顆粒状物質（ＴＫ−１）と白色顆粒状物
質（ＴＫ−２）（６６０倍）Fig. 2 Black granular substance (TK-1) and white granular substance (TK-2) (660 times)
【図 ３】耐熱性粒子状の物質（ＴＲ−１）と、発熱性
粒子状の物質（ＴＲ−２）他、ＴＫ−１、Ｋ−２
（６６０倍）FIG. 3: Heat-resistant particulate matter (TR-1), exothermic particulate matter (TR-2), TK-1, K-2, etc.
(660 times)
【図 ４】米糠を２２０℃加熱、蒸留水に溶かしたとき
の黒色顆粒状物質（ＴＫ−１）
（６６０倍）[Fig. 4] Black granular substance (TK-1) obtained by heating rice bran at 220 ° C and dissolving it in distilled water.
(660 times)
【図 ５】２２０℃加熱時の白色顆粒状物質（ＴＫ−
２）と、耐熱性状物質（ＴＲ−１）
（６６０倍）FIG. 5: White granular substance (TK-
2) and a heat resistant substance (TR-1)
(660 times)
【図 ６】２２０℃加熱時の黒色顆粒状物質（ＴＫ−
１）と、発熱性物質（ＴＲ−２）
（６６０倍）FIG. 6: Black granular material (TK-
1) and exothermic substance (TR-2)
(660 times)
【図 ７】粘性系物質の集落
（２００倍）[Fig. 7] Village of viscous substances
(200 times)
【図 ８】粘性系物質の集落と黒色顆粒状物質の成長過
程 （２００倍）[Fig. 8] Colony of viscous substance and growth process of black granular substance (200 times)
【図 ９】白色系物質の集落
（２００倍）[Figure 9] White substance settlement
(200 times)
【図 １０】赤色系物質の集落
（２００倍）[Figure 10] Village of red-based substances
(200 times)
【図 １１】黄色系物質の集落
（２００倍）[Figure 11] Yellow-based material settlement
(200 times)
【図 １２】発熱性物質（ＴＲ−２）の状態
（６６０倍）FIG. 12 State of exothermic substance (TR-2)
(660 times)
【図 １３】発熱性物質（ＴＲ−２）と、耐熱性物質
（ＴＲ−１） （６６０倍）FIG. 13: Exothermic substance (TR-2) and heat-resistant substance (TR-1) (660 times)
Claims (15)
すその上清には黒色顆粒状の物質（略式記号 ＴＫ−１
とする）の存在が認められる。1. A rice bran is weighed (10 g) and dissolved in distilled water. As a supernatant thereof, a black granular substance (abbreviated symbol TK-1) is used.
The presence of
（凍結・解凍）をするその上清には黒色顆粒状の物質の
存在が確認された。2. The presence of a black granular substance was confirmed in the supernatant obtained by (freezing / thawing) the solution obtained by the same method as in (claim 1).
拌棒にて撹拌しインキュベーターにて５０℃及び７４℃
にて２４時間以上静置培養をする。その上清にはＴＫ−
１，白色（光沢を及びる）顆粒状の物質（略式記号 Ｔ
Ｋ−２）の存在及び耐熱性粒子状の物質（略式記号 Ｔ
Ｒ−１）発熱性粒子状の物質（略式記号 ＴＲ−２）の
存在が確認された。3. A solution obtained by the same method as in (claim 1) is stirred with a stirring rod and heated at 50 ° C. and 74 ° C. in an incubator.
Culture for 24 hours or more. The supernatant contains TK-
1, white (glossy) granular substance (abbreviated symbol T
K-2) and heat-resistant particulate matter (abbreviated symbol T
R-1) The presence of an exothermic particulate substance (abbreviated symbol TR-2) was confirmed.
拌後１００℃２０分煮沸する。その上清からＴＫ−１、
ＴＫ−２、ＴＲ−１、ＴＲ−２の存在が確認された。4. The solution obtained by the same method as in (claim 1) is stirred and boiled at 100 ° C. for 20 minutes. TK-1, from the supernatant
Presence of TK-2, TR-1, and TR-2 was confirmed.
ートクレーブ（型式ＨＡ−２４０ＭＩＩ：平山製作所）
を用いて１２１℃、１．６気圧３０分間高温にさらす。
その上清からＴＫ−１、ＴＫ−２、ＴＲ−１、ＴＲ−２
の存在が確認された。5. A solution obtained by the same method as (claim 1) is autoclaved (model HA-240MII: Hirayama Seisakusho).
Exposed to high temperature at 121 ° C. and 1.6 atmospheres for 30 minutes.
From the supernatant, TK-1, TK-2, TR-1, TR-2
Was confirmed.
ートクレーブ（平山製作所）を用いて１２３℃、１．６
気圧４０分間高温にさらす。その上清からＴＫ−１、Ｔ
Ｋ−２、ＴＲ−１、ＴＲ−２の存在が確認された。6. The solution obtained by the same method as (claim 1) is heated at 123 ° C. and 1.6 ° C. using an autoclave (Hirayama Seisakusho).
Expose to high temperature for 40 minutes. TK-1, T from the supernatant
The presence of K-2, TR-1, and TR-2 was confirmed.
山製作所の協力を得てオートクレーブで１５８℃、４．
９気圧、３０分間高温高圧にさらす。その上清からＴＫ
−１、ＴＲ−２、ＴＲ−１の存在を確認する。但し、３
日後 ＴＫ−２の存在も認められた。また、オートクレ
ーブ後の溶液は、７４℃恒温器内（型式ＩＳ４２−Ｎ、
ヤマト科学社）に保存。7. The solution obtained by the same method as (claim 1) is autoclaved at 158 ° C. with the cooperation of Hirayama Seisakusho.
Exposing to high temperature and high pressure for 30 minutes at 9 atm. TK from the supernatant
Confirm the existence of -1, TR-2, TR-1. However, 3
After day, the presence of TK-2 was also recognized. In addition, the solution after autoclaving was in an incubator at 74 ° C (type IS42-N,
Saved by Yamato Scientific Co., Ltd.).
アルミホイルを袋状にして密閉する。（二袋用意） 乾熱滅菌器 （型式ＳＴ−６０、平山製作所）を用いて
１９０℃〜２００℃、３０分 加熱する。ベージュ色の
米糠が炭化状態の黒褐色に変色した。一部蒸留水に溶か
し顕鏡下にてＴＫ−１，ＴＫ−２及びＴＲ−１、ＴＲ−
２を確認した。8. Rice bran is weighed (10 g) and the doubled aluminum foil is sealed in a bag shape. (Preparation of two bags) Using a dry heat sterilizer (model ST-60, Hirayama Seisakusho), heat at 190 ° C to 200 ° C for 30 minutes. The beige rice bran turned carbonized black brown. Partially dissolved in distilled water and examined under a microscope TK-1, TK-2 and TR-1, TR-
2 was confirmed.
したアルミホイルを袋状にして密閉、また、皿状にして
開放、これを２００℃〜２２０℃、２０分 加熱する。
密閉状は黒褐色、開放状は黒色ともに灰（炭化状態）で
ある一部蒸留水に溶解する。ＴＫ−１，ＴＫ−２、ＴＲ
−１，ＴＲ−２ともに確認した。9. Rice and rice bran are weighed (10 g), and the doubled aluminum foil is bag-shaped and closed, and is also plate-shaped and opened, and this is heated at 200 ° C. to 220 ° C. for 20 minutes.
Both the closed state is blackish brown and the open state is black, which is ash (carbonized state) and partially dissolved in distilled water. TK-1, TK-2, TR
Both -1 and TR-2 were confirmed.
０ｍｌに溶解し上清を１０倍稀釈後その１ｍｌを９０ｍ
ｍΦシヤーレ上に１０％ＰＤＡ（ポテトデキュストガス
寒天）培地上に撒く、３７℃、７４℃インキュベーター
（ヤマト科学社）に設置、ＴＫ−１，ＴＫ−２、ＴＲ−
１、ＴＲ−２が確認された。10. Rice and rice bran are weighed (10 g) and distilled water 9
Dissolve in 0 ml and dilute the supernatant 10 times and then 1 ml of it to 90 m
Scatter on 10% PDA (potato decousto gas agar) medium on mΦ shear, install in 37 ° C, 74 ° C incubator (Yamato Scientific Co., Ltd.), TK-1, TK-2, TR-
1, TR-2 was confirmed.
て恒温状態で３日以降寒天上にＴＫ−１，及びＴＫ−２
の集落（コロニー）を、又は樹々状態として確認された
（３７℃インキュベーター）ＴＲ−１、ＴＲ−２におい
ては３７℃インキュベーターにおいては認められなかっ
たが７４°ｃにては顕著である。ともに集落をつくらな
い。ＴＲ−１においては一定以上、形状的に大きくなら
ないがＴＲ−２はより大きく、発熱の状態が認められ
る。11. TK-1 and TK-2 are placed on agar for 3 days or more in a constant temperature in an incubator (claim 10).
In the case of TR-1 and TR-2, which were confirmed as colonies or as a tree state (37 ° C. incubator), they were not observed in the 37 ° C. incubator, but they were remarkable at 74 ° c. We will not create a village together. In TR-1, the shape does not become larger than a certain amount, but in TR-2, it is larger, and a heat generation state is recognized.
９０ｍｌに溶解し１００°ｃ、２０分間煮沸をする。上
清を１０倍稀釈後１ｍｌを（請求項１０）と同方法で撒
く。７４°ｃインキュベーターに設置（請求項１１）を
同様な状態を確認する。12. Rice and rice bran are weighed (10 g), dissolved in 90 ml of distilled water and boiled at 100 ° C. for 20 minutes. After diluting the supernatant 10 times, 1 ml is spread in the same manner as in (claim 10). Install in a 74 ° c incubator (Claim 11) and check for similar conditions.
温状態にて１０日以降には、単位同志が融合しあい大型
化する（ＴＲ−２）同時にＴＲ−２の予備群の成長過程
を認める。これら融合体の中心部分あるいは、その周辺
からドーム状の形を認める。内部は密集しているものも
あり空洞のものも認められる。13. The same as in claim 12, after 10 days at a constant temperature of 74 ° C., the unites of each unit are fused and become large (TR-2), and at the same time, the growth process of the preliminary group of TR-2. Admit. A dome-shaped form is recognized from the central part of these fusions or its periphery. Some of them are dense and some are hollow.
パン・ハイテック（株）の協力を得て中温、高温感知顕
微鏡（型式ＴＨ−６００ＰＭ、ジャパンハイテック）に
て測定の結果共に２００．７°ｃを越えて安定している
のが認められた。なお、上限を検討中14. The results of both TR-1 and TR-2 measured with a medium- and high-temperature sensing microscope (Model TH-600PM, Japan High-Tech) with the cooperation of Japan High-Tech Co., Ltd. are both 200.7 °. It was found to be stable beyond c. The upper limit is under consideration
５０°ｃのインキュベーターに設置、３日後に上清を遠
心機（型式Ｈ−５０１、国産遠心機）を用い４０００
ｒ．ｐ．ｍ．１０分（室温）で上清と沈渣物とに分け
る。上清にはＴＲ−１、ＴＲ−２のＴＲ系があり沈渣物
にはＴＫ−１、ＴＫ−２のＴＫ系がある。ＴＲ系を８０
００ｒ．ｐ．ｍ．２０分にても沈降せずミリポアフィル
ター、ポアサイズ０．４５ｍｍを用いて吸着させ、フィ
ルターについたＴＲ系を温水にて解離する。15. Large-scale culture of rice and rice bran 500 g of rice bran is weighed, dissolved in 4.5 l of distilled water, stirred, placed in an incubator at 50 ° C., and after 3 days, the supernatant is centrifuged (model H-501, domestically produced). Centrifuge) 4000
r. p. m. Separate into supernatant and sediment at 10 minutes (room temperature). The TR-1 and TR-2 TR systems are present in the supernatant, and the TK-1 and TK-2 TK systems are present in the sediment. TR system 80
00r. p. m. It does not settle even after 20 minutes and is adsorbed using a Millipore filter, pore size 0.45 mm, and the TR system attached to the filter is dissociated with warm water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10867793A JPH07187A (en) | 1993-03-31 | 1993-03-31 | Classification of substance extracted from rice and rice bran |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10867793A JPH07187A (en) | 1993-03-31 | 1993-03-31 | Classification of substance extracted from rice and rice bran |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH07187A true JPH07187A (en) | 1995-01-06 |
Family
ID=14490880
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP10867793A Pending JPH07187A (en) | 1993-03-31 | 1993-03-31 | Classification of substance extracted from rice and rice bran |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH07187A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0799964A (en) * | 1993-10-01 | 1995-04-18 | Chika:Kk | Surface and internal structure of SRT by electron microscope and its characteristics |
| JPH07111889A (en) * | 1993-10-19 | 1995-05-02 | Chika:Kk | Reaction of SRT in vitro and in vivo in animals and main components and characteristics of SRT |
| JP2005333851A (en) * | 2004-05-25 | 2005-12-08 | Tsuno Rice Fine Chemicals Co Ltd | Fermentation promoter |
| US8885262B2 (en) | 2001-05-14 | 2014-11-11 | Olympus Corporation | Electronic image pickup system |
-
1993
- 1993-03-31 JP JP10867793A patent/JPH07187A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH0799964A (en) * | 1993-10-01 | 1995-04-18 | Chika:Kk | Surface and internal structure of SRT by electron microscope and its characteristics |
| JPH07111889A (en) * | 1993-10-19 | 1995-05-02 | Chika:Kk | Reaction of SRT in vitro and in vivo in animals and main components and characteristics of SRT |
| US8885262B2 (en) | 2001-05-14 | 2014-11-11 | Olympus Corporation | Electronic image pickup system |
| JP2005333851A (en) * | 2004-05-25 | 2005-12-08 | Tsuno Rice Fine Chemicals Co Ltd | Fermentation promoter |
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