JPH07255305A - Method for culturing plant tissue or seedling material - Google Patents
Method for culturing plant tissue or seedling materialInfo
- Publication number
- JPH07255305A JPH07255305A JP8220494A JP8220494A JPH07255305A JP H07255305 A JPH07255305 A JP H07255305A JP 8220494 A JP8220494 A JP 8220494A JP 8220494 A JP8220494 A JP 8220494A JP H07255305 A JPH07255305 A JP H07255305A
- Authority
- JP
- Japan
- Prior art keywords
- plant tissue
- incubator
- plant
- free medium
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000012258 culturing Methods 0.000 title claims abstract description 15
- 238000000034 method Methods 0.000 title claims description 9
- 239000000463 material Substances 0.000 title abstract 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 13
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 13
- 239000005708 Sodium hypochlorite Substances 0.000 claims abstract description 9
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000003242 anti bacterial agent Substances 0.000 claims description 5
- 241000196324 Embryophyta Species 0.000 abstract description 37
- 244000052616 bacterial pathogen Species 0.000 abstract description 4
- 240000008415 Lactuca sativa Species 0.000 abstract description 2
- 235000012045 salad Nutrition 0.000 abstract description 2
- 239000002609 medium Substances 0.000 description 24
- 241000894006 Bacteria Species 0.000 description 15
- 239000001963 growth medium Substances 0.000 description 6
- 238000011109 contamination Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 238000007796 conventional method Methods 0.000 description 3
- 230000009545 invasion Effects 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 235000000346 sugar Nutrition 0.000 description 3
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 2
- 229930182566 Gentamicin Natural products 0.000 description 2
- 206010021033 Hypomenorrhoea Diseases 0.000 description 2
- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- 108010039918 Polylysine Proteins 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229960002518 gentamicin Drugs 0.000 description 2
- 229940049954 penicillin Drugs 0.000 description 2
- 229920000656 polylysine Polymers 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 244000003416 Asparagus officinalis Species 0.000 description 1
- 235000005340 Asparagus officinalis Nutrition 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 241000612152 Cyclamen hederifolium Species 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 241000234435 Lilium Species 0.000 description 1
- 241000233855 Orchidaceae Species 0.000 description 1
- 241001505935 Phalaenopsis Species 0.000 description 1
- 241000109329 Rosa xanthina Species 0.000 description 1
- 235000004789 Rosa xanthina Nutrition 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 230000001651 autotrophic effect Effects 0.000 description 1
- 229930186364 cyclamen Natural products 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 229910017053 inorganic salt Inorganic materials 0.000 description 1
- 239000011490 mineral wool Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 229960005076 sodium hypochlorite Drugs 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 235000021012 strawberries Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Landscapes
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
Description
【０００１】[0001]
【産業上の利用分野】本発明は植物組織又は幼植物体の
培養方法に関する。植物組織又は幼植物体を培養器内で
培養し、培養して得られた植物体を培養器から取り出し
て栽培することが行なわれている。本発明は上記のよう
な植物組織又は幼植物体の培養方法の改良に関するもの
である。BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for culturing plant tissue or seedlings. BACKGROUND ART Plant tissues or young plants are cultivated in an incubator, and the plants obtained by culturing are taken out from the incubator and cultivated. The present invention relates to an improvement in the method for culturing plant tissues or seedlings as described above.
【０００２】[0002]
【従来の技術】従来、植物組織又は幼植物体の培養方法
として一般に、植物組織又は幼植物体を、糖類を含有す
る有糖培地を入れた培養器内で従属栄養培養することが
行なわれている。ところが、かかる従来法には、その性
質上、培地が雑菌汚染を受け易く、これにより植物組織
又は幼植物体の培養に重大な支障をきたすという問題が
ある。2. Description of the Related Art Conventionally, as a method for cultivating a plant tissue or a young plant, generally, a plant tissue or a young plant is heterotrophically cultured in an incubator containing a sugar-containing medium containing sugars. There is. However, such a conventional method has a problem that the medium is apt to be contaminated with various bacteria due to its nature, which seriously hinders the culture of plant tissues or seedlings.
【０００３】そこで従来、植物組織又は幼植物体の培養
方法として、植物組織又は幼植物体を、糖類を含有しな
い無糖培地を入れた培養器内で光独立栄養培養すること
が提案されている（特開昭５４−５２７８７、特開平１
−１６５３１５、特開平４−２６２７２０、特開平５−
１５３６３）。ところが、かかる従来法でも、実際のと
ころ、培地が雑菌汚染を受け、これにより植物組織又は
幼植物体の培養に重大な支障をきたすという問題があ
る。Therefore, as a method for culturing plant tissues or seedlings, it has been conventionally proposed to photoautotrophically culture the plant tissues or seedlings in an incubator containing a sugar-free medium containing no sugar. (JP-A-54-52787, JP-A-1
165315, JP-A-4-262720, JP-A-5-
15363). However, even with such a conventional method, there is a problem that the medium is contaminated with various bacteria, which seriously hinders the culture of plant tissues or seedlings.
【０００４】植物組織又は幼植物体は培養器内で培養さ
れ、該培養器は培養室内に置かれる。しかし、培養器は
相応に密封されているものの、完全に気密化されている
というわけではなく、また培養室内の雰囲気は相応に菌
管理がなされているものの、完全に無菌化されていると
いうわけでもない。実際のところは、培養中に、空気中
に浮遊している雑菌が培養器内へ侵入するのを避けられ
ないのである。特に、培養した幼植物体を他の培養器へ
移植するに際しては、これをクリーンベンチ内で行なう
としても、作業者や使用する道具に付着している雑菌が
培養器内へ侵入し易い。培養器内へ雑菌が侵入し、繁殖
すると、植物組織又は幼植物体の培養に重大な支障をき
たす。The plant tissue or seedling is cultured in an incubator, and the incubator is placed in a culture chamber. However, although the incubator is correspondingly sealed, it is not completely airtight, and the atmosphere in the culture chamber is appropriately sterilized, although the bacteria are controlled accordingly. not. As a matter of fact, it is inevitable that various bacteria floating in the air enter the incubator during the culture. In particular, when transplanting a cultured seedling to another incubator, even if it is carried out in a clean bench, various bacteria adhering to the worker or the tool used are likely to enter the incubator. If miscellaneous bacteria invade the incubator and propagate, it seriously hinders the culture of plant tissues or seedlings.
【０００５】[0005]
【発明が解決しようとする課題】本発明が解決しようと
する課題は、植物組織又は幼植物体を無糖培地で光独立
栄養培養する従来法でも、培養器内へ雑菌が侵入して繁
殖するのを避けられず、これにより植物組織又は幼植物
体の培養に重大な支障をきたす点である。The problem to be solved by the present invention is that, even in the conventional method of photoautotrophically culturing plant tissues or seedlings in a sugar-free medium, various bacteria invade and propagate in the incubator. Is unavoidable, which seriously hinders the culture of plant tissues or seedlings.
【０００６】[0006]
【課題を解決するための手段】しかして本発明者らは、
上記の課題を解決するべく研究した結果、培養器内へ雑
菌が侵入するのを避けられない実情の下では、培養器内
へ雑菌が侵入しても、それが繁殖するのを抑制すること
が肝要であり、そのためには、植物組織又は幼植物体を
無糖培地で光独立栄養培養するが、この際に所定のｐＨ
域に調整した無糖培地を用いるのが有効であることを見
出した。However, the present inventors have
As a result of research to solve the above-mentioned problems, under the circumstance that invasion of various bacteria into the incubator is unavoidable, even if invasion of various bacteria into the incubator, it is possible to suppress the propagation thereof. It is essential that a plant tissue or a young plant is photoautotrophically cultured in a sugar-free medium at this time.
It was found that it is effective to use a sugar-free medium adjusted to the range.
【０００７】すなわち本発明は、植物組織又は幼植物体
を、ｐＨ３．０〜４．５に調整した無糖培地で、光照射
下及び炭酸ガス含有空気雰囲気下に培養することを特徴
とする植物組織又は幼植物体の培養方法に係る。That is, the present invention is a plant characterized in that a plant tissue or a young plant is cultivated in a sugar-free medium adjusted to pH 3.0 to 4.5 under light irradiation and carbon dioxide-containing air atmosphere. The present invention relates to a method for culturing a tissue or a young plant.
【０００８】本発明において対象となる植物組織又は幼
植物体は、サラダ菜、アスパラガス等の野菜類、イチ
ゴ、メロン等の果実類、ラン、バラ、ユリ、シクラメン
等の花卉類の組織（細胞）又は該組織を培養した幼植物
体（幼苗）である。In the present invention, the target plant tissues or seedlings are tissues (cells) of salad vegetables, vegetables such as asparagus, fruits such as strawberries and melons, orchids, roses, lilies and cyclamen. Alternatively, it is a young plant (young seedling) obtained by culturing the tissue.
【０００９】本発明では、培養器に無糖培地を入れ、該
無糖培地で植物組織又は幼植物体を光独立栄養培養す
る。培養器は透明のガラス製又はプラスチックス製の密
封可能な光透過性のもので且つガス交換が可能なものを
用いる。かかる培養器に無糖培地を入れる。無糖培地は
固体培地又は液体培地のどちらでもよいが、液体培地が
好ましい。追肥に便利であるからである。無糖培地は、
無機塩を含有する、ｐＨ３．０〜４．５に調整したもの
を用いる。ｐＨ３．０未満では、侵入した雑菌の繁殖を
抑制することはできるが、植物組織又は幼植物体の培養
それ自体に支障をきたし、逆にｐＨ４．５超では、侵入
した雑菌の繁殖を充分に抑制することができない。In the present invention, a sugar-free medium is placed in the incubator, and plant tissues or seedlings are photoautotrophically cultured in the sugar-free medium. The incubator is made of transparent glass or plastics, which can be sealed and has a light-transmitting property, and which can exchange gas. A sugar-free medium is placed in the incubator. The sugar-free medium may be either a solid medium or a liquid medium, but a liquid medium is preferred. This is because it is convenient for top dressing. The sugar-free medium is
The one containing an inorganic salt and adjusted to pH 3.0 to 4.5 is used. If the pH is less than 3.0, the proliferation of invading bacteria can be suppressed, but the culture itself of plant tissues or seedlings will be disturbed. On the contrary, if the pH exceeds 4.5, the proliferation of invading bacteria will be sufficiently suppressed. Cannot be suppressed.
【００１０】無糖培地は抗菌剤を添加したものを用いる
のがより有効である。かかる抗菌剤としては、ポリリジ
ン、ペニシリン、ゲンタマイシン、次亜塩素酸ナトリウ
ム等があり、ポリリジンの場合は０．１〜１０％の濃度
となるように、またペニシリンの場合は１０〜１００ｐ
ｐｍの濃度となるように、更にゲンタマイシンの場合は
１〜１００ｐｐｍの濃度となるように添加したものを用
いるが、次亜塩素酸ナトリウムを０．２５〜１０ｐｐｍ
の濃度となるように添加したものを用いるのが好まし
い。これらの抗菌剤は侵入した雑菌の繁殖を抑制すると
いうよりもむしろ侵入した時点で雑菌を殺すのに効果が
あり、この意味で次亜塩素酸ナトリウムはより効果があ
る。次亜塩素酸ナトリウムを添加する場合、その濃度が
０．２５ｐｐｍ未満では充分な効果が得られず、逆に１
０ｐｐｍ超では植物組織又は幼植物体の培養それ自体に
支障をきたす。It is more effective to use a sugar-free medium to which an antibacterial agent is added. Examples of such antibacterial agents include polylysine, penicillin, gentamicin, sodium hypochlorite, etc., so that the concentration of polylysine is 0.1 to 10%, and that of penicillin is 10 to 100 p.
In order to obtain a pm concentration, and in the case of gentamicin, those added so as to have a concentration of 1 to 100 ppm are used, but sodium hypochlorite is added in an amount of 0.25 to 10 ppm.
It is preferable to use the one added so that the concentration becomes. These antibacterial agents are effective in killing germs at the time of invasion rather than suppressing the growth of germs invading, and sodium hypochlorite is more effective in this sense. When sodium hypochlorite is added, if the concentration is less than 0.25 ppm, a sufficient effect cannot be obtained.
If it exceeds 0 ppm, the culture itself of the plant tissue or the seedling body is disturbed.
【００１１】植物組織又は幼植物体を、ｐＨ３．０〜
４．５に調整した無糖培地で、好ましくは更に次亜塩素
酸ナトリウムを０．２５〜１０ｐｐｍの濃度となるよう
に添加した無糖培地で、光照射下及び炭酸ガス含有空気
雰囲気下に光独立栄養培養する。光独立栄養培養が可能
な条件であれば、光強度及び炭酸ガス濃度は特に制限さ
れないが、光強度２０〜５００μmol／ｍ2／ｓの光照射
下及び炭酸ガス濃度３００〜７０００ｐｐｍの炭酸ガス
含有空気雰囲気下に光独立栄養培養するのが好ましい。
植物組織又は幼植物体の培養を促すことができるからで
ある。培養器内の炭酸ガス濃度は、培養器内の雰囲気ガ
スの換気、培養器内への炭酸ガス含有空気の通気によっ
て保持することができる。Plant tissues or seedlings are adjusted to pH 3.0 to
A sugar-free medium adjusted to 4.5, preferably a sugar-free medium to which sodium hypochlorite was further added so as to have a concentration of 0.25 to 10 ppm, was exposed to light and under a carbon dioxide-containing air atmosphere. Autotrophic culture. The light intensity and the carbon dioxide concentration are not particularly limited as long as photoautotrophic culture is possible, but the light intensity and the carbon dioxide-containing air having a carbon dioxide concentration of 300 to 7,000 ppm are irradiated under light irradiation of 20 to 500 μmol / m 2 / s. It is preferable to perform photoautotrophic culture in an atmosphere.
This is because it is possible to promote the cultivation of plant tissues or young plants. The carbon dioxide concentration in the incubator can be maintained by ventilation of atmospheric gas in the incubator and aeration of carbon dioxide-containing air into the incubator.
【００１２】[0012]
試験区分１ 培養器として、容量３００mlの三角フラスコに１００ml
の粒状ロックウールを入れ、ゴム栓で密栓したものを用
いた。ゴム栓には直径１０mmの孔を開け、該孔に除菌フ
ィルタを被着した。培地として、ｐＨ２．５、３．０、
３．５、４．０、４．５、５．０又は５．５に調整し
た、合計７区分の、無機塩のみを含有するＭＳ液体培地
を用いた。培養器及び合計７区分の各培地をオートクレ
ーブで滅菌した後、開栓して、培養器に各培地を８０ml
入れた。クリーンベンチ内又は培養室内で、培養器に別
に培養しておいた発芽後５か月目の胡蝶蘭の幼苗を１６
苗移植し、密栓した。合計７区分の各培地毎に、同様の
移植をそれぞれ１００個行なった。移植後、各培養器を
培養室内に置き、室内の雰囲気の炭酸ガス濃度を５００
ｐｐｍに保持し、２５℃で、明期の光強度が２５μmol
／ｍ2／ｓである１２時間明期の条件下に、４か月間培
養した。培養後、合計７区分の各培地毎に、雑菌汚染の
発生率（％）を調査し、その結果を表１に示した。また
培養後に雑菌汚染が認められなかったものについて、合
計７区分の各培地毎に、幼苗の活着率（％）及び平均生
体重（ｇ）を調査し、その結果を表２に示した。Test Category 1 100 ml in a 300 ml Erlenmeyer flask as an incubator
The granular rock wool of No. 1 was put in and tightly sealed with a rubber stopper. A hole having a diameter of 10 mm was opened in the rubber stopper, and a sterilizing filter was attached to the hole. As the medium, pH 2.5, 3.0,
A total of 7 categories of MS liquid medium containing only inorganic salts adjusted to 3.5, 4.0, 4.5, 5.0 or 5.5 was used. After sterilizing the incubator and each culture medium of 7 categories in total by autoclave, open the cap and incubate 80 ml of each culture medium.
I put it in. 16 seedlings of Phalaenopsis orchid 5 months after germination that had been separately cultivated in an incubator in a clean bench or in a culture room
Seedlings were transplanted and sealed. 100 similar transplants were performed for each of the 7 culture media in total. After transplantation, place each incubator in the culture room and adjust the carbon dioxide concentration in the atmosphere to 500
The light intensity in the light period is 25 μmol at 25 ° C.
The cells were cultured for 4 months under the condition of 12-hour light period of / m 2 / s. After culturing, the incidence (%) of contamination by various bacteria was investigated for each of the 7 culture media in total, and the results are shown in Table 1. In addition, regarding the cultures in which no contamination of bacteria was observed after the culture, the survival rate (%) and the average fresh weight (g) of the seedlings were examined for each of the 7 culture media in total, and the results are shown in Table 2.
【００１３】[0013]
【表１】 [Table 1]
【００１４】[0014]
【表２】 [Table 2]
【００１５】試験区分２ オートクレーブで滅菌した後の、ｐＨ４．５に調整した
ＭＳ液体培地に、次亜塩素酸ナトリウムを、０．１、
０．２５、０．５、１．０、５．０、１０．０又は１
５．０ｐｐｍとなるように添加し、次亜塩素酸ナトリウ
ム０ｐｐｍの無添加区を含め、合計８区分の培地を用い
たこと以外は試験区分１の場合と同様に移植し、培養し
た。培養後、合計８区分の各培地毎に、雑菌汚染の発生
率（％）を調査し、その結果を表３に示した。また培養
後に雑菌汚染が認められなかったものについて、合計８
区分の各培地毎に、幼苗の活着率（％）及び平均生体重
（ｇ）を調査し、その結果を表４に示した。Test Category 2 After sterilization in an autoclave, sodium hypochlorite (0.1) was added to an MS liquid medium adjusted to pH 4.5.
0.25, 0.5, 1.0, 5.0, 10.0 or 1
It was added to 5.0 ppm, and transplanted and cultured in the same manner as in Test Category 1 except that a total of 8 categories of media were used, including a non-addition group of 0 ppm sodium hypochlorite. After culturing, the incidence (%) of contamination by various bacteria was investigated for each of the 8 culture media in total, and the results are shown in Table 3. In addition, the total of 8 items was found for which no contamination of bacteria was observed after culturing.
The survival rate (%) of seedlings and the average fresh weight (g) were investigated for each medium of each section, and the results are shown in Table 4.
【００１６】[0016]
【表３】 [Table 3]
【００１７】[0017]
【表４】 [Table 4]
【００１８】[0018]
【発明の効果】既に明らかなように、以上説明した本発
明には、植物組織又は幼植物体を無糖培地で光独立栄養
培養するに際し、培養器内へ雑菌が侵入しても、その繁
殖を抑制して、植物組織又は幼植物体の培養を円滑に行
なうことができるという効果がある。EFFECTS OF THE INVENTION As is apparent, the present invention described above, in the photoautotrophic culture of plant tissues or seedlings in a sugar-free medium, even if germs enter the incubator, they propagate Is suppressed, and the plant tissue or young plant can be smoothly cultured.
Claims (4)
４．５に調整した無糖培地で、光照射下及び炭酸ガス含
有空気雰囲気下に培養することを特徴とする植物組織又
は幼植物体の培養方法。1. A plant tissue or a young plant having a pH of 3.0 to.
A method for cultivating a plant tissue or a young plant, which comprises culturing in a sugar-free medium adjusted to 4.5 under light irradiation and an atmosphere of carbon dioxide-containing air.
求項１記載の植物組織又は幼植物体の培養方法。2. The method for cultivating a plant tissue or a young plant according to claim 1, which comprises culturing in a sugar-free medium to which an antibacterial agent is added.
０．２５〜１０ｐｐｍの濃度となるように添加した無糖
培地で培養する請求項２記載の植物組織又は幼植物体の
培養方法。3. The method for culturing plant tissue or seedling according to claim 2, wherein the culture is carried out in a sugar-free medium to which sodium hypochlorite is added as an antibacterial agent so as to have a concentration of 0.25 to 10 ppm.
照射下及び炭酸ガス濃度３００〜７０００ｐｐｍの炭酸
ガス含有空気雰囲気下に培養する請求項１、２又は３記
載の植物組織又は幼植物体の培養方法。4. The plant tissue or young plant according to claim 1, 2 or 3, which is cultivated under light irradiation with a light intensity of 20 to 500 μmol / m 2 / s and in a carbon dioxide-containing air atmosphere with a carbon dioxide concentration of 300 to 7000 ppm. How to culture the body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8220494A JPH07255305A (en) | 1994-03-28 | 1994-03-28 | Method for culturing plant tissue or seedling material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8220494A JPH07255305A (en) | 1994-03-28 | 1994-03-28 | Method for culturing plant tissue or seedling material |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH07255305A true JPH07255305A (en) | 1995-10-09 |
Family
ID=13767901
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8220494A Pending JPH07255305A (en) | 1994-03-28 | 1994-03-28 | Method for culturing plant tissue or seedling material |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH07255305A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1070450A1 (en) | 1999-07-21 | 2001-01-24 | Nisshinbo Industries, Inc. | Method for plant tissue culture |
| KR100340728B1 (en) * | 2000-06-21 | 2002-06-20 | 윤여중 | Methods And Compositions For Controlling Fungi And Bacteria During Plant Tissue Culture |
| WO2005058022A1 (en) * | 2003-12-19 | 2005-06-30 | Yongtai Zhang | Combination-type plant sugarless tissue culture propagation device and method thereof |
| CN102726290A (en) * | 2011-04-12 | 2012-10-17 | 山东省烟台农业学校 | Method for eliminating tissue culture seedling pollution |
| JP2013111024A (en) * | 2011-11-29 | 2013-06-10 | Excel Clean Techno Co Ltd | Gel medium for cultivating plant and method of cultivating plant |
| WO2020203947A1 (en) * | 2019-03-29 | 2020-10-08 | キリンホールディングス株式会社 | Plant accumulating useful substance |
-
1994
- 1994-03-28 JP JP8220494A patent/JPH07255305A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1070450A1 (en) | 1999-07-21 | 2001-01-24 | Nisshinbo Industries, Inc. | Method for plant tissue culture |
| KR100340728B1 (en) * | 2000-06-21 | 2002-06-20 | 윤여중 | Methods And Compositions For Controlling Fungi And Bacteria During Plant Tissue Culture |
| WO2005058022A1 (en) * | 2003-12-19 | 2005-06-30 | Yongtai Zhang | Combination-type plant sugarless tissue culture propagation device and method thereof |
| CN102726290A (en) * | 2011-04-12 | 2012-10-17 | 山东省烟台农业学校 | Method for eliminating tissue culture seedling pollution |
| JP2013111024A (en) * | 2011-11-29 | 2013-06-10 | Excel Clean Techno Co Ltd | Gel medium for cultivating plant and method of cultivating plant |
| WO2020203947A1 (en) * | 2019-03-29 | 2020-10-08 | キリンホールディングス株式会社 | Plant accumulating useful substance |
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