JPH07330505A - Antimicrobial composition - Google Patents

Abstract

PURPOSE:To obtain an antimicrobial composition capable of providing sufficient antimicrobial activity without using germicides and preservatives conventionally used, having low toxicity and irritation and not causing allergy. CONSTITUTION:This antimicrobial composition contains at least two kinds of substances or at least one kind of substance selected from a group composed of a pH adjuster, preferably citric acid or its salt and an antimicrobial substance and pH of the composition is adjusted to 2.8-7.0, preferably 3.5-6.5. The antimicrobial substance excludes germicides and preservatives having possibility giving damage to human body, and a substance derived from an animal or its extract, a substance derived from a plant or its extract or synthetic antimicrobial substance is exemplified as the antimicrobial substance and especially, an antimicrobial polyol (e.g. 1,3-butylene glycol or dipropylene glycol) or phenoxyethanol is preferably used as the antimicrobial substance and the blend amount is preferably 2.0-5.0wt.% in antimicrobial polyol and preferably 0.1-1.0wt.% in phenoxyethanol, respectively.

Description

Detailed Description of the Invention

[0001]

This invention relates to an antibacterial composition,
In particular, it relates to an antibacterial composition that can be suitably used for cosmetics, pharmaceuticals, foods and the like.

[0002]

2. Description of the Related Art Currently, in various fields, prevention of contamination or deterioration of products caused by microorganisms,
Various bactericides and preservatives are used as preservatives (or preservatives) to ensure the storability of products. Among these, in the field of products directly applied to or ingested by the living body, particularly in the fields of cosmetics, pharmaceuticals, foods, etc., in order to ensure the safety of the living body, the types and application amounts of preservatives that can be used Is often set. For example,
In the field of cosmetics, the preservatives are benzoic acid (0.2%), benzoates (1.0%), salicylic acid (0.2%), salicylates (1.0%), carboxylic acid (0.1%) according to the Ministry of Health and Welfare notification.
%), Sorbic acid and its salts (0.5%), dehydroacetic acid and its salts (0.5%), paraoxybenzoic acid ester (1.0%), parachlorometacresol (0.5%)
%), Bithionol (0.1%), hexachlorophene (0.1%), boric acid (0.5%) and resorcinol (0.1%)
%) (The maximum concentration used in parentheses), and these are also mandatory ingredients. In the field of pharmaceuticals,
Benzoic acid, sodium benzoate, benzalkonium chloride, benzethonium chloride, cresol, chlorobutanol, thymol, methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, butyl paraoxybenzoate, phenol and benzyl alcohol as preservatives It is listed in the Japanese Pharmacopoeia. Further, in the food field, substances having toxicity cannot be used, and benzoic acid and its sodium salt, sorbic acid and its potassium salt, dehydroacetic acid and its sodium salt, paraoxybenzoic acid and its ester are used as preservatives. (Ethyl, propyl, isopropyl,
Butyl, isobutyl), propionic acid and its sodium or calcium salts, diphenyl, orthophenylphenol and thiabendazole.

However, even if the kind and the amount used are limited, many of these substances are essentially allergens causing skin and systemic allergies, and application of these substances causes allergies. There is a fear. In addition, many of them are irritating or toxic.

Therefore, attempts have been made so far to impart antibacterial activity without using such bactericides and preservatives. For example, a method of blending alcohol, a method of blending phenoxyethanol, and a method of using antimicrobial polyol. A method using an antibacterial animal or plant-derived component or extract is known. In addition, it is generally known that the antibacterial property is enhanced in the acidic region of pH 2.5 or lower and the alkaline region of pH 10.5 or higher. Boosting techniques are widely used.

[0005]

However, the method of blending these alcohols, phenoxyethanol, antibacterial polyols, antibacterial extracts and the like cannot obtain sufficient antibacterial activity, and at room temperature, it may rot during use, Mold will grow. Therefore, the composition produced by using these methods, aseptic manufacturing so as not to be mixed with microorganisms at the time of manufacture, and stored in a container or a disposable type small container in which microorganisms are difficult to mix, refrigerated after opening. We cope with the lack of antibacterial activity by methods such as storage and short-term use.

Further, a method of making the composition acidic (pH 2.5 or less) or alkaline (pH 10.5 or more) can impart sufficient antibacterial activity to the composition, but such a composition is It is highly irritating to the living body, especially the skin and mucous membranes, and cannot be applied to general compositions except for special applications.

Therefore, the present invention does not use a bactericidal agent or an antiseptic agent which is toxic or irritating, or which may be an allergen causing allergies, which may harm the living body, particularly the human body. It is an object of the present invention to provide a hypoallergenic, hypoallergenic, and low-toxicity antibacterial composition capable of retaining an antibacterial activity equivalent to or higher than those obtained by using these fungicides and preservatives for a long period of time.

[0008]

In view of the above circumstances, the present inventors have made earnest studies and, as a result, have used at least one antibacterial substance other than a bactericide or a preservative under a specific pH condition, or The inventors have found that it is possible to obtain a practically sufficient antibacterial activity by using at least two kinds of antibacterial substances other than bactericides or antiseptics in combination, and completed the present invention.

That is, the antibacterial composition according to one aspect of the present invention is a composition containing at least two kinds selected from the group consisting of a pH adjuster and an antibacterial substance,
The pH adjuster is characterized in that the pH of the composition is 2.8 to 7.0. The antibacterial substances used here are preferably phenoxyethanol and antibacterial polyols.

An antibacterial composition according to another aspect of the present invention contains at least one antibacterial substance, and p
Characterized by H being 2.8 to 7.0.

The term "antibacterial substance" as used herein is conventionally widely used in the fields of cosmetics, pharmaceuticals, foods, etc., and although it is possible to impart sufficient antibacterial activity to the composition by itself, it is toxic. It refers to an antibacterial substance excluding bactericides and preservatives, which have an irritating property or become an allergen that causes allergies, which may harm the living body, especially the human body. In the present invention, the antibacterial substance may be a naturally occurring substance or a synthetic substance. Specifically, as an example of a naturally derived antibacterial substance, beeswax, propolis, royal jelly, milk sperm, horse oil, hydroxyacetic acid, chitin, as an animal-derived substance or extract,
Chitosan, peptides and the like can be mentioned, and as a plant-derived substance or extract, aloe, sardine, eucalyptus, pearl oyster, neem grass, kujin, ginger ginger, hawthorn, sanshin, ginseng, shikon, perilla, sycamore, sowakuhi, touki. , Carrot, nindou, garlic, bakumondo, juniper, loquat, peach, yukinoshita, forsythia, logwood, arnica,
Hypericum, chamomile, salvia, peony, ginger, birch, yarrow, senkyu, thyme, quince, hamamelis, hop, mugwort,
Kawamurawoge, lavender, lemon and rosemary extract, licorice flavonoids, α-bisabolol,
Examples thereof include peptides. Examples of synthetic antibacterial substances include ethanol, lower alcohols such as isopropanol, antibacterial polyols such as 1,3-butylene glycol, dipropylene glycol, and 3-methyl-1,3-butanediol, phenoxyethanol, α- A hydroxy acid etc. can be mentioned. The preferred antibacterial substance in the present invention is an antibacterial polyol or phenoxyethanol.

The blending amount of these antibacterial substances differs depending on the dosage form and prescription of the composition, and is determined in consideration of antibacterial activity, safety, feel, appearance, physical properties, etc., and can be generally specified. However, the total amount of antibacterial substances used is generally 0.0101 to 100.0% by weight, based on the total composition. Further, for each antibacterial substance, beeswax is 0.1 to 50.0 with respect to the entire composition, preferably
0.5 to 5.0% by weight, 0.01 to 99.9 in propolis, preferably 0.1 to 10.0% by weight, 0.01 to 99 in royal jelly.
9, preferably 0.1-10.0% by weight, for milk semen 0.1-99.
9, preferably 0.1-50.0% by weight, for horse oil 0.1-99.
9, 0.1-99.0 wt%, 0.01-99.9, preferably 0.1-99.0 wt% for peptides, 0.01-99.9, preferably 0.1-99.0 wt% for chitin, 0.01-99.9 for chitosan, preferably 0.1-99.0 wt%, In various plant-derived extracts
0.01-99.9, licorice flavonoids 0.0001-5.0,
Preferably 0.001-1.0% by weight, for α-bisabolol
0.1-2.0, preferably 0.1-1.0 wt%, lower alcohol 1.0-99.9, preferably 2.0-50.0 wt%, antimicrobial polyol 1.0-99.9, preferably 2.0-50.0
% By weight, 0.1-5.0% by weight for phenoxyethanol, preferably 0.1-1.0% by weight, 0.01-2 for α-hydroxy acids.
0.0, preferably 0.01 to 5.0% by weight, for pH adjusters
It is 0.01 to 20.0, preferably 0.01 to 5.0% by weight.

The antibacterial composition of the present invention contains at least two kinds of substances selected from the above antibacterial substances,
Alternatively, it contains at least one substance selected from the above antibacterial substances under the condition of pH 2.8 to 7.0.

In the present invention, the pH of the composition is 2.8.
A pH adjuster can be used to achieve ~ 7.0. The pH adjuster used has a composition pH of 2.8 to
Any one can be used as long as it can be adjusted to 7.0, preferably pH 3.5 to 6.5, and those usually used in each field can be used. The composition may have a pH within the above range, but it is preferably lower from the viewpoint of antibacterial property. P used in this invention
The H-adjusting agent is preferably an inorganic acid or an organic acid or a salt thereof, and examples of the organic acid include citric acid, glycolic acid, succinic acid, tartaric acid, lactic acid, malic acid, butyric acid and oxalic acid, and inorganic acid. Examples thereof include phosphoric acid and hydrochloric acid, and sodium salts, potassium salts, calcium salts and the like of these acids can be preferably used. Particularly preferably, the pH adjusting agent is citric acid or a salt thereof.

When the pH of the composition inevitably falls within the above range due to the antibacterial substance or the formulation constituents of the composition, it is not necessary to use a pH adjuster.

The blending amount of these pH adjusting agents is the p of the composition.
The amount may be a sufficient amount to adjust H to 2.8 to 7.0, and the optimum compounding amount is determined by the dosage form and formulation of the composition.
Therefore, although it cannot be specified unconditionally,
It is 0.01 to 5.0% by weight with respect to the entire composition.

In the antibacterial composition according to the present invention,
In addition to the above-mentioned components, any additives usually used in each field can be added, but components that promote the growth of microorganisms are preferably limited to the extent that the antibacterial activity of the composition is not impaired.

Examples of the form of the antibacterial composition according to the present invention include water-containing type, powder type, solid powder type and oil type. The water-containing composition may be any composition as long as it contains water, and examples thereof include an emulsification type, a solubilization type, a solution type, a dispersion type and a gel type.

The antibacterial composition according to the present invention can be used in any field, but particularly in the fields of cosmetics, pharmaceuticals, foods, etc., it can be preferably used as a base material or carrier. In addition, diseases related to microorganisms and allergies, such as acne, dandruff, armpits, inflammation,
It can also be used to improve symptoms such as athlete's foot and atopy.
Further, it can be used for disinfection and sterilization.

[0020]

EXAMPLES Hereinafter, the antibacterial composition according to the present invention will be described in more detail with reference to Examples.

Examples 1 to 5 and Comparative Examples 1 to 3 Lotions having the respective compositions shown in Table 1 below were prepared according to the production method described in A) below. Next, the antibacterial activity of the obtained lotion was measured using E. coli and Aspergillus niger by the method described in B) below. The results are also shown in Table 1.

A) Manufacturing method All components of each composition shown in Table 1 were weighed, heated to about 80 ° C. and uniformly mixed, and then cooled to obtain a sample. The operation was performed aseptically.

B) Method for measuring antibacterial activity (1) Test using Escherichia coli Homogeneous cells were obtained from one colony by pre-preculture 36 hours, preculture 24 hours and main culture 5 hours. Then, the cells were washed by centrifugation and washed with phosphate buffered saline.
It was adjusted to × 10 ⁸ [CFU] to give an inoculum solution. afterwards,
This inoculum solution was added to each sample prepared in A) above in an amount of 1% and kept in a water bath at 37 ° C to measure the viable cell count over time.

Preconditions Pre-preculture / preculture: SCD agar, 37 ° C main culture: SCD broth, 50 rpm rec
iprocal, 37 ° C Test strain: Escherichia coli IAM 12119 Viable count medium: SCDLP agar The antibacterial activity is determined based on the lotion (several) of a commercially available product containing a bactericidal / preservative agent as follows. I did.

Antibacterial activity equal to or higher than that of the commercial product: ◎ Antibacterial activity equivalent to that of the commercial product: ○ Antibacterial activity lower than that of the commercial product or bacteria growing: × (2) Test using black mold The conidia obtained are harvested and 0.01% Twee
n 80 3 Dispersed in physiological saline, filtered, and filtered 3 × 10 ⁷
The inoculum solution was adjusted to spore / ml. Add 1% of this inoculum to each sample prepared in A) above,
The cells were kept in an incubator to measure the viable cell count over time and the growth of hyphae was observed.

Implementation conditions Sporulation medium: MY medium Test strain: Aspergillus niger IFO 4407 Medium for viable cell count: GPLP agar To determine the antibacterial activity, use lotion (s) of a commercial product containing a bactericidal / preservative agent. As a reference, the procedure was as follows.

Antibacterial activity equal to or higher than commercial products: ◎ Antibacterial activity equivalent to commercial products: ○ Antibacterial properties, but inferior to commercial products: △ No antibacterial properties, germs germinate and multiply: ×

[Table 1] As is clear from Table 1, the antibacterial composition according to the present invention exhibits superior antibacterial activity to both E. coli and Aspergillus niger, which is equal to or higher than that of a commercial product containing a bactericidal / preservative. On the other hand, none of the compositions of Comparative Examples had a sufficient antibacterial activity and were not at a level that could withstand commercial use.

Example 6 A cream having the following formulation was prepared according to the following production method.

Treatment Mixing amount (% by weight) ------------------------------ (1) Beeswax 5.0 (2) Behenyl alcohol 4.0 (3) Liquid paraffin 20.0 (4) Glycerin monostearate 5.0 (5) Polyoxyethylene sorbitan monostearate 3.0 (6) Phenoxyethanol 0.5 (7) Citric acid 0.02 (8) 1,3-Butylene glycol 7.0 (9) Purified water 55.48 −−−−−−−−−−−−−−−−−−−−−−−−−−−−−−− --- pH 3.8 manufacturing method) (i) (1) to (6) of the above formulation are heated and dissolved at 75 ° C.

(Ii) (7) to (9) of the above formulation separately from (i)
Is melted by heating at 75 ° C.

(Iii) The preparation of (ii) is added to the preparation of (i) above, and the mixture is emulsified and cooled.

Example 7 A beauty essence having the following formulation was prepared according to the following production method.

Treatment Mixing amount (% by weight) ----------------------- (1) Plant extract 1.0 (2) Sorbit 3.0 (3) Glycerin 3 .0 (4) sodium hyaluronate 0.03 (5) xanthan gum 1.0 (6) phenoxyethanol 0.4 (7) citric acid 0.2 (8) 1,3-butylene glycol 10.0 (9) purified water 81.37 −−−−−−−−−−−−−−−−−−−−−−−−−− pH 4.8 Manufacturing method) (i) (1) to (3) and (3) of the above formulation. 6)-(9) are heated and melted at 70 ℃.

(Ii) (4) and (5) of the above formulation are added to the preparation of (i) with stirring, uniformly dissolved, and cooled.

Example 8 A cleaning agent having the following formulation was prepared according to the following production method.

Method Blending amount (% by weight) --------------------------- (1) Lauroyl-L-Glutamic acid triethanol 60. 0 (2) Coconut oil fatty acid amide propyl betaine 10.0 (3) Allantoin 0.1 (4) Citric acid 0.3 (5) Phenoxyethanol 0.5 (6) Purified water 29.1 -------- -------------------------------- pH 5.3 Manufacturing method) (1) to (7) of the above formulation are stirred at room temperature to uniformly dissolve.

Example 9 A gel having the following formulation was prepared according to the following production method.

Method Mixing amount (% by weight) ------------------ (1) Carboxyvinyl polymer (1% aq.) 60. 0 (2) Collagen (0.3%) 2.0 (3) Sodium hyaluronate (1%) 5.0 (4) Allantoin 0.1 (5) Plant extract 3.0 (6) Phenoxyethanol 0.4 (7) ) 1,3-Butylene glycol 10.0 (8) Purified water 19.5 ------------------------------ pH 6. 3 Manufacturing method) (1) to (8) of the above formulation are stirred and uniformly dissolved.

Example 10 A cream having the following formulation was prepared according to the following production method.

Treatment Mixing amount (% by weight) ------------------------------------- (1) Beeswax 5.0 (2) Behenyl alcohol 4.0 (3) Adsorption purified lanolin 15.0 (4) Liquid paraffin 25.0 (5) Sodium stearate 5.0 (6) Polyoxyethylene sorbitan monostearate 3.0 (7) 1,3- Butylene glycol 7.0 (8) Purified water 36.0 −−−−−−−−−−−−−−−−−−−−−−−−−−−−−− pH 4.0 Manufacturing method (I) (1) to (6) of the above formulation are heated and dissolved at 75 ° C.

(Ii) Apart from (i), (7) and
(8) is melted by heating at 75 ° C.

(Iii) The preparation of (ii) is added to the preparation of (i), and the mixture is emulsified and cooled.

Example 11 A milk lotion having the following formulation was prepared according to the following method.

Treatment Mixing amount (% by weight) ------------------------------ (1) Stearic acid 4.0 (2) ) Behenyl alcohol 4.0 (3) Liquid paraffin 5.0 (4) Monoglycerin stearate 4.0 (5) Polyoxyethylene sorbitan monostearate 2.0 (6) Phenoxyethanol 0.3 (7) Citric acid 0.0. 1 (8) Sodium citrate 0.1 (9) 1,3-butylene glycol 6.0 (10) Purified water 74.5 −−−−−−−−−−−−−−−−−−−−−− --------------- pH 4.6 Manufacturing Method) (i) (1) to (6) of the above formulation are heated and dissolved at 75 ° C.

(Ii) In addition to (i), (7) to (1
0) is heated and melted at 75 ° C.

(Iii) The preparation of (ii) is added to the preparation of (i), and the mixture is emulsified and cooled.

The antibacterial activity of the beauty essences, detergents, gels, creams and milk lotions prepared in Examples 6 to 11 was determined in the same manner as in Examples 1 and 2 above. The results are shown in Table 2 below.

Table 2 Actual Example -------------------------------- 6 7 8 9 10 11 -------------------- −−−−−−−−−−−−−−− Antibacterial activity against E. coli ○ ◎ ○ ○ ○ ◎ Antibacterial activity against Aspergillus niger ◎ ◎ ◎ ◎ ○ ○ ◎ As is clear from Table 2, the antibacterial composition according to the present invention. The product shows excellent antibacterial activity equivalent to or higher than that of a commercial product containing a sterilizing / preservative agent against both Escherichia coli and Aspergillus niger. In addition, these compositions also include Pseudomonas aeruginosa,
Excellent antibacterial activity against Staphylococcus aureus and Bacillus subtilis.

[0049]

As described above, according to the present invention, a hypoallergenic, hypoallergenic and low-toxicity antibacterial composition is provided. This antibacterial composition has toxicity or irritation, or becomes an allergen that causes allergies,
It exerts sufficient antibacterial activity without the use of conventional bactericides and preservatives that may harm the living body.

The antibacterial composition according to the present invention is a cosmetic product,
It can be suitably used for medicines, foods, etc., and particularly when used for cosmetics, it is possible to obtain sufficient antibacterial activity without containing a sterilizing / preservative agent that is stipulated by the Ministry of Health and Welfare notification and has a labeling obligation. Therefore, it can be referred to as "additive-free cosmetics", and is not subject to restrictions such as expiration date, container to be used, need for refrigerated storage, and the like.

Claims (3)

[Claims] 1. An antibacterial composition containing at least two kinds of substances selected from the group consisting of a pH adjusting agent and an antibacterial substance, wherein the pH adjusting agent adjusts the pH of the composition to 2.8 to 7.0. An antibacterial composition which is 2. An antibacterial composition containing at least one antibacterial substance and having a pH of 2.8 to 7.0. 3. At least 2 selected from the group consisting of pH adjusters, phenoxyethanol and antibacterial polyols.
An antibacterial composition containing different types of substances, wherein the pH adjuster adjusts the pH of the composition to 2.8 to 7.0.