JPH0734759B2 - 体液中の抗トロンビン▲ｉｉｉ▼を測定する方法及び試薬 - Google Patents

体液中の抗トロンビン▲ｉｉｉ▼を測定する方法及び試薬

Info

Publication number: JPH0734759B2
Authority: JP; Japan
Prior art keywords: thrombin; pro; arg; gly; substrate
Prior art date: 1989-07-14
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired - Fee Related

Application number

JP2185456A

Other languages

English (en)

Japanese (ja)

Other versions

JPH0353898A (ja

Inventor

アンドレアス・デサウエル

ラインハート・ヘルツ

ヘルムート・リル

Original Assignee

ベーリンガー・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1989-07-14

Filing date

1990-07-16

Publication date

1995-04-19

1990-07-16 Application filed by ベーリンガー・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング filed Critical ベーリンガー・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング

1991-03-07 Publication of JPH0353898A publication Critical patent/JPH0353898A/ja

1995-04-19 Publication of JPH0734759B2 publication Critical patent/JPH0734759B2/ja

2010-04-19 Anticipated expiration legal-status Critical

Status Expired - Fee Related legal-status Critical Current

Links

102000004411 Antithrombin III Human genes 0.000 title claims abstract description 18
108090000935 Antithrombin III Proteins 0.000 title claims abstract description 18
229960005348 antithrombin iii Drugs 0.000 title claims abstract description 18
210000001124 body fluid Anatomy 0.000 title claims abstract description 7
239000010839 body fluid Substances 0.000 title claims abstract description 7
238000000034 method Methods 0.000 title claims description 17
239000003153 chemical reaction reagent Substances 0.000 title claims description 11
108090000190 Thrombin Proteins 0.000 claims abstract description 46
229960004072 thrombin Drugs 0.000 claims abstract description 46
239000000758 substrate Substances 0.000 claims abstract description 28
230000035945 sensitivity Effects 0.000 claims abstract description 8
WXPZDDCNKXMOMC-AVGNSLFASA-N (2s)-1-[(2s)-2-[[(2s)-1-(2-aminoacetyl)pyrrolidine-2-carbonyl]amino]-5-(diaminomethylideneamino)pentanoyl]pyrrolidine-2-carboxylic acid Chemical compound NCC(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1[C@H](C(O)=O)CCC1 WXPZDDCNKXMOMC-AVGNSLFASA-N 0.000 claims abstract description 7
108010038807 Oligopeptides Proteins 0.000 claims abstract description 7
102000015636 Oligopeptides Human genes 0.000 claims abstract description 7
108010017446 glycyl-prolyl-arginyl-proline Proteins 0.000 claims abstract description 7
239000003593 chromogenic compound Substances 0.000 claims abstract description 6
108010018472 chromozym TH Proteins 0.000 claims abstract description 6
VYLJFJGMPYBPMN-VXKWHMMOSA-N (2s)-n-[(2s)-5-(diaminomethylideneamino)-1-(4-nitroanilino)-1-oxopentan-2-yl]-1-[2-[(4-methylphenyl)sulfonylamino]acetyl]pyrrolidine-2-carboxamide Chemical compound C1=CC(C)=CC=C1S(=O)(=O)NCC(=O)N1[C@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)NC=2C=CC(=CC=2)[N+]([O-])=O)CCC1 VYLJFJGMPYBPMN-VXKWHMMOSA-N 0.000 claims abstract description 5
239000003795 chemical substances by application Substances 0.000 claims abstract description 4
108090000765 processed proteins & peptides Proteins 0.000 claims description 3
102000004169 proteins and genes Human genes 0.000 claims description 3
108090000623 proteins and genes Proteins 0.000 claims description 3
125000004432 carbon atom Chemical group C* 0.000 claims description 2
125000000217 alkyl group Chemical group 0.000 claims 1
238000006243 chemical reaction Methods 0.000 abstract description 7
239000000523 sample Substances 0.000 description 14
239000004019 antithrombin Substances 0.000 description 7
239000003398 denaturant Substances 0.000 description 7
238000005259 measurement Methods 0.000 description 7
XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 5
239000004202 carbamide Substances 0.000 description 5
239000000243 solution Substances 0.000 description 5
230000015572 biosynthetic process Effects 0.000 description 4
108090000790 Enzymes Proteins 0.000 description 3
102000004190 Enzymes Human genes 0.000 description 3
HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 3
108091005804 Peptidases Proteins 0.000 description 3
239000004365 Protease Substances 0.000 description 3
208000007536 Thrombosis Diseases 0.000 description 3
238000004458 analytical method Methods 0.000 description 3
230000023555 blood coagulation Effects 0.000 description 3
229940088598 enzyme Drugs 0.000 description 3
229960002897 heparin Drugs 0.000 description 3
229920000669 heparin Polymers 0.000 description 3
239000012898 sample dilution Substances 0.000 description 3
239000012488 sample solution Substances 0.000 description 3
239000000126 substance Substances 0.000 description 3
239000013026 undiluted sample Substances 0.000 description 3
TYMLOMAKGOJONV-UHFFFAOYSA-N 4-nitroaniline Chemical compound NC1=CC=C([N+]([O-])=O)C=C1 TYMLOMAKGOJONV-UHFFFAOYSA-N 0.000 description 2
ONIBWKKTOPOVIA-SCSAIBSYSA-N D-Proline Chemical compound OC(=O)[C@H]1CCCN1 ONIBWKKTOPOVIA-SCSAIBSYSA-N 0.000 description 2
KZSNJWFQEVHDMF-SCSAIBSYSA-N D-valine Chemical compound CC(C)[C@@H](N)C(O)=O KZSNJWFQEVHDMF-SCSAIBSYSA-N 0.000 description 2
102000009123 Fibrin Human genes 0.000 description 2
108010073385 Fibrin Proteins 0.000 description 2
BWGVNKXGVNDBDI-UHFFFAOYSA-N Fibrin monomer Chemical compound CNC(=O)CNC(=O)CN BWGVNKXGVNDBDI-UHFFFAOYSA-N 0.000 description 2
102000035195 Peptidases Human genes 0.000 description 2
230000002411 adverse Effects 0.000 description 2
230000000694 effects Effects 0.000 description 2
229950003499 fibrin Drugs 0.000 description 2
239000012085 test solution Substances 0.000 description 2
VJZRBVVLWLEXBB-VROPFNGYSA-N (2s)-n-[(2s)-5-(diaminomethylideneamino)-1-(4-nitroanilino)-1-oxopentan-2-yl]-1-[2-[(4-methylphenyl)sulfonylamino]acetyl]pyrrolidine-2-carboxamide;hydrochloride Chemical compound Cl.C1=CC(C)=CC=C1S(=O)(=O)NCC(=O)N1[C@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)NC=2C=CC(=CC=2)[N+]([O-])=O)CCC1 VJZRBVVLWLEXBB-VROPFNGYSA-N 0.000 description 1
KZZWQCKYLNIOBT-UHFFFAOYSA-N 5-amino-2-nitrobenzoic acid Chemical compound NC1=CC=C([N+]([O-])=O)C(C(O)=O)=C1 KZZWQCKYLNIOBT-UHFFFAOYSA-N 0.000 description 1
102100027522 Baculoviral IAP repeat-containing protein 7 Human genes 0.000 description 1
101710177963 Baculoviral IAP repeat-containing protein 7 Proteins 0.000 description 1
229930182820 D-proline Natural products 0.000 description 1
229930182831 D-valine Natural products 0.000 description 1
108010049003 Fibrinogen Proteins 0.000 description 1
102000008946 Fibrinogen Human genes 0.000 description 1
241000042032 Petrocephalus catostoma Species 0.000 description 1
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
239000007983 Tris buffer Substances 0.000 description 1
230000004913 activation Effects 0.000 description 1
230000001154 acute effect Effects 0.000 description 1
-1 alkyl radical Chemical class 0.000 description 1
238000003556 assay Methods 0.000 description 1
125000001584 benzyloxycarbonyl group Chemical group C(=O)(OCC1=CC=CC=C1)* 0.000 description 1
230000027455 binding Effects 0.000 description 1
239000008280 blood Substances 0.000 description 1
210000004369 blood Anatomy 0.000 description 1
239000000872 buffer Substances 0.000 description 1
238000011088 calibration curve Methods 0.000 description 1
230000015556 catabolic process Effects 0.000 description 1
GKTWGGQPFAXNFI-HNNXBMFYSA-N clopidogrel Chemical compound C1([C@H](N2CC=3C=CSC=3CC2)C(=O)OC)=CC=CC=C1Cl GKTWGGQPFAXNFI-HNNXBMFYSA-N 0.000 description 1
230000015271 coagulation Effects 0.000 description 1
238000005345 coagulation Methods 0.000 description 1
150000001875 compounds Chemical class 0.000 description 1
238000004132 cross linking Methods 0.000 description 1
238000006731 degradation reaction Methods 0.000 description 1
238000001514 detection method Methods 0.000 description 1
230000006866 deterioration Effects 0.000 description 1
238000007865 diluting Methods 0.000 description 1
201000010099 disease Diseases 0.000 description 1
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
208000009190 disseminated intravascular coagulation Diseases 0.000 description 1
229940079593 drug Drugs 0.000 description 1
239000003814 drug Substances 0.000 description 1
230000002255 enzymatic effect Effects 0.000 description 1
229940012952 fibrinogen Drugs 0.000 description 1
229960000789 guanidine hydrochloride Drugs 0.000 description 1
PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
230000023597 hemostasis Effects 0.000 description 1
230000001900 immune effect Effects 0.000 description 1
208000019423 liver disease Diseases 0.000 description 1
WCYWZMWISLQXQU-UHFFFAOYSA-N methyl Chemical compound [CH3] WCYWZMWISLQXQU-UHFFFAOYSA-N 0.000 description 1
239000003607 modifier Substances 0.000 description 1
239000000178 monomer Substances 0.000 description 1
LPVNCTNQHLHKAD-UHFFFAOYSA-N n-methoxy-n-phenylnitramide Chemical compound CON([N+]([O-])=O)C1=CC=CC=C1 LPVNCTNQHLHKAD-UHFFFAOYSA-N 0.000 description 1
102000004196 processed proteins & peptides Human genes 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
238000005303 weighing Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2337/00—N-linked chromogens for determinations of peptidases and proteinases
- C12Q2337/10—Anilides
- C12Q2337/12—Para-Nitroanilides p-NA
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/81—Protease inhibitors
- G01N2333/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- G01N2333/811—Serine protease (E.C. 3.4.21) inhibitors
- G01N2333/8121—Serpins
- G01N2333/8128—Antithrombin III
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/974—Thrombin

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Engineering & Computer Science (AREA)
Microbiology (AREA)
Neurosurgery (AREA)
Biotechnology (AREA)
Immunology (AREA)
Analytical Chemistry (AREA)
Molecular Biology (AREA)
Physics & Mathematics (AREA)
Biophysics (AREA)
Hematology (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Investigating Or Analysing Biological Materials (AREA)

JP2185456A 1989-07-14 1990-07-16 体液中の抗トロンビン▲ｉｉｉ▼を測定する方法及び試薬 Expired - Fee Related JPH0734759B2 (ja)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
DE3923340A DE3923340A1 (de)	1989-07-14	1989-07-14	Verfahren und reagenz zur bestimmung von antithrombin iii
DE3923340.5		1989-07-14

Publications (2)

Publication Number	Publication Date
JPH0353898A JPH0353898A (ja)	1991-03-07
JPH0734759B2 true JPH0734759B2 (ja)	1995-04-19

Family

ID=6385054

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP2185456A Expired - Fee Related JPH0734759B2 (ja)	1989-07-14	1990-07-16	体液中の抗トロンビン▲ｉｉｉ▼を測定する方法及び試薬

Country Status (8)

Country	Link
EP (1)	EP0408075B1 (de)
JP (1)	JPH0734759B2 (de)
AT (1)	ATE119578T1 (de)
AU (1)	AU622339B2 (de)
DD (1)	DD298444A5 (de)
DE (2)	DE3923340A1 (de)
ES (1)	ES2069635T3 (de)
ZA (1)	ZA905489B (de)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
AU628960B2 (en) *	1989-04-07	1992-09-24	Teijin Limited	Method of immunologically assaying human thrombin- antithrombin III complex, assay reagent and kit therefor
JPH078298A (ja) *	1993-06-28	1995-01-13	Nippon Shoji Kk	アンチトロンビンｉｉｉ活性の測定方法および該測定用試薬キット
DE59703340D1 (de) *	1996-08-17	2001-05-17	Aventis Behring Gmbh	Verfahren zur Quantifizierung von Glykosaminoglykanen in Antithrombin III-haltigen Lösungen
US6165795A (en) *	1998-06-25	2000-12-26	Cardiovascular Diagnostics, Inc.	Methods for performing fibrinogen assays using dry chemical reagents containing ecarin and magnetic particles
DE102005003145B4 (de)	2005-01-21	2006-10-19	Dade Behring Marburg Gmbh	Stablies, chromogenes Flüssigreagenz und dessen Verwendung in gerinnungsdiagnostischen Tests
GB0914883D0 (en)	2009-08-26	2009-09-30	Univ Belfast	Compound

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE3005540A1 (de) *	1980-02-14	1981-08-20	Boehringer Mannheim Gmbh, 6800 Mannheim	Verfahren und reagens zur bestimmung der biologischen aktivitaet von heparin im plasma
US4473639A (en) *	1982-09-15	1984-09-25	Miles Laboratories, Inc.	Reagent strip test for antithrombin-III
DE3244030A1 (de) *	1982-11-27	1984-05-30	Behringwerke Ag, 3550 Marburg	Chromogene verbindungen, verfahren zu ihrer herstellung und ihre verwendung
DE3811647A1 (de) *	1988-04-07	1989-10-26	Behringwerke Ag	Verfahren und verpackung enthaltend mittel zur kinetischen bestimmung von faktor xiii

1989
- 1989-07-14 DE DE3923340A patent/DE3923340A1/de not_active Withdrawn
1990
- 1990-07-11 AU AU58885/90A patent/AU622339B2/en not_active Ceased
- 1990-07-12 DD DD90342731A patent/DD298444A5/de not_active IP Right Cessation
- 1990-07-13 AT AT90113483T patent/ATE119578T1/de active
- 1990-07-13 DE DE59008621T patent/DE59008621D1/de not_active Expired - Fee Related
- 1990-07-13 ZA ZA905489A patent/ZA905489B/xx unknown
- 1990-07-13 EP EP90113483A patent/EP0408075B1/de not_active Expired - Lifetime
- 1990-07-13 ES ES90113483T patent/ES2069635T3/es not_active Expired - Lifetime
- 1990-07-16 JP JP2185456A patent/JPH0734759B2/ja not_active Expired - Fee Related

Also Published As

Publication number	Publication date
JPH0353898A (ja)	1991-03-07
ZA905489B (en)	1991-04-24
ES2069635T3 (es)	1995-05-16
EP0408075A3 (en)	1992-02-26
AU622339B2 (en)	1992-04-02
EP0408075B1 (de)	1995-03-08
DE59008621D1 (de)	1995-04-13
EP0408075A2 (de)	1991-01-16
AU5888590A (en)	1991-01-17
DE3923340A1 (de)	1991-01-24
ATE119578T1 (de)	1995-03-15
DD298444A5 (de)	1992-02-20

Legal Events

Date	Code	Title	Description
2004-04-06	R250	Receipt of annual fees	Free format text: JAPANESE INTERMEDIATE CODE: R250
2005-04-05	R250	Receipt of annual fees	Free format text: JAPANESE INTERMEDIATE CODE: R250
2006-04-04	R250	Receipt of annual fees	Free format text: JAPANESE INTERMEDIATE CODE: R250
2007-04-19	LAPS	Cancellation because of no payment of annual fees

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US4234682A (en)	1980-11-18	Method and reagent for determining biologically active heparin in plasma
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US5292664A (en)	1994-03-08	Functional test and reagent for determining fibrinogen
JPH0368680B2 (de)	1991-10-29
US4598043A (en)	1986-07-01	Method for quantitatively assaying blood coagulation factor XII in human plasma
US4918001A (en)	1990-04-17	Method for the determination of protease inhibitors
EP0657547B1 (de)	2001-04-18	Verfahren zur messung der antithrombin-iii-aktivität
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US5320945A (en)	1994-06-14	Method and reagent for the determination of antithrombin III
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