JPH085706Y2 - Bone marrow transplant bag - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial application] The present invention relates to a bone marrow transplant bag for purifying bone marrow fluid collected from a donor and donating it to other patients, and particularly to the upper and lower ends of a bag-shaped body. This is a bag for bone marrow transplantation in which a bone marrow introduction tube and a bone marrow extraction tube are provided and the bottom of the main body is formed into a funnel shape so that the bone marrow fluid can be easily purified.

[Prior Art] At present, a radiation treatment method, a treatment method using an anticancer agent, or the like is attempted for blood cancer such as leukemia, but it has been extremely difficult to cure it. Recently, however, the bone marrow transplantation method has attracted attention and various clinical treatments have been performed.

The success or failure of the bone marrow transplantation method is to carefully select donors that can be used for bone marrow transplantation. Thoroughly examine biocompatibility with hematological cancer patients. Concentrate and purify fresh bone marrow fluid from donors in a short time. Etc.

Currently, the bone marrow transplant bag 51 shown in FIG. 3 is used, and the bone marrow fluid was concentrated and purified by the following operation, for example.

A predetermined amount of bone marrow fluid is collected from the donor's hip bone or the like using a syringe or the like and stored in a bag 51 shown in FIG.

The bag 51 is centrifuged to separate it into plasma (upper layer), buffy coat containing bone marrow (middle layer) and red blood cells (lower layer) (Fig. 4).

The red blood cells in the layer below the transfer tube 52 are discharged from the bag 51.

The bag 51 [plasma (upper layer), bone marrow-containing buffy coat (lower layer)] is subjected to centrifugation to re-spin the bone marrow fluid on the bottom of the bag 51.

The bone marrow fluid is discharged from the transfer tube 52 and stored in a predetermined container.

The physiological saline solution is introduced into the bag 51 from which the bone marrow fluid has been discharged and washed to collect the bone marrow fluid without waste.

Combine the bone marrow fluid with and further centrifuge,
Form dense bone marrow.

[Problems to be Solved by the Invention] However, in the bag 51, when performing the above-mentioned centrifugation and washing, the plasma layer (upper layer) which is an unnecessary component (when the plasma layer (upper layer) is discharged first, red blood cells are discharged. (Lower layer) interferes with the separation of the bone marrow fluid, and in order to obtain pure bone marrow fluid, the above centrifugation and washing operations had to be repeated. Therefore, it has been difficult to concentrate and purify the bone marrow fluid in a fresh state in a short time.

Therefore, the present inventor has made intensive studies in order to solve the above problems, and as a result, arrived at the next inventor.

[Means for Solving the Problems] The present invention provides a bone marrow introducing tube at the upper end of a flexible bag-shaped body,
A bone marrow transplant bag in which a bone marrow discharge tube is provided at the lower end of the main body and the bottom of the main body is formed in a funnel shape is provided.

[Example] FIG. 1 shows a schematic view of a bag 1 for bone marrow transplantation of the present invention. The bone marrow transplant bag 1 is composed of a flexible bag-shaped main body 2, a bone marrow introducing tube 3 and a bone marrow discharging tube 4, and the bone marrow introducing tube 3 is provided at the upper end of the main body 2 and A connector 5 is attached to the tip. The connector 5 is for aseptically injecting the bone marrow fluid collected from a donor with a syringe into the main body 2. The other bone marrow discharge tube 4 is provided at the lower end of the main body 2, and a seal portion 8 is formed at the tip thereof. The seal portion 8 can withstand gravity generated at the time of centrifugal separation, which will be described later,
At the same time, the bone marrow introduction tube 3 so as not to damage the bag 1
Is formed by welding with a high frequency welder, for example.

Further, the bottom of the bag-shaped body 2 is formed into a funnel shape so that the plasma (upper layer) after centrifugation, the buffy coat containing bone marrow (middle layer), and red blood cells (lower layer) can be separated more efficiently. There is. The funnel angle θ can be appropriately adjusted depending on the viscosity of the liquid and the like.

Further, at the upper end of the main body 2, liquid outlet ports 6, 6 are provided.
Are formed in plural, and these are covered by protectors 7, 7.

Next, an example of a method of using the bone marrow transplant bag 1 of the present invention will be described.

Bone marrow fluid is collected from the donor's hip bone using a syringe or the like, and is introduced into the bag-shaped main body 2 through the connector 5 and the bone marrow introduction tube 3 to collect the bone marrow fluid in the bone marrow transplant bag 1. . The bone marrow introducing tube 3 is sealed by a high-frequency welder or the like on the way and separated from the main body together with the connector 5. The main body 2 is the remaining bone marrow introduction tube 3
And a seal portion (same as the seal portion 8) formed at the tip thereof.

The bag 1 is subjected to a centrifugal separation treatment to separate it into plasma (upper layer), buffy coat containing bone marrow (middle layer) and red blood cells (lower layer) as shown in FIG.

From the bag 1, the lower layer red blood cells are discharged from the bone marrow discharge tube 4 by using, for example, the aseptic connecting means described in Japanese Patent Publication No. Sho 61-30582, and collected in a predetermined container. Highly viscous red blood cells are rapidly discharged from the bottom of the funnel-shaped main body 2 by spontaneous fall. Since the interface between the buffy coat and red blood cells becomes narrow at the bottom of the main body, it is easy to separate the buffy coat and red blood cells.

The upper layer plasma is discharged from the bag 1 [plasma (upper layer), buffy coat containing bone marrow (lower layer)] through the bone marrow introducing tube 3.

The buffy coat containing the bone marrow of the above bag 1 is subjected to centrifugal separation to re-spin the bone marrow fluid, and the bone marrow fluid is discharged from the bone marrow discharge tube 4 using, for example, the aseptic connecting means of JP-B-61-30582. And store in a predetermined container. The highly viscous bone marrow fluid is quickly discharged from the bottom of the funnel-shaped main body 2 by spontaneous fall. Since the interface between the bone marrow fluid and the other components is narrowed at the bottom of the body 2, the bone marrow fluid can be easily separated. The physiological saline solution is introduced into the bag 1 from which the bone marrow fluid has been discharged and washed, and the bone marrow fluid is collected without waste in the same manner as described above.

Combine the bone marrow fluid with and further centrifuge,
Purify concentrated bone marrow. Since the tips of the bone marrow introducing tube 3 and the bone marrow ejecting tube 4 are only sealed by the seal portion 4 (since there are no extra obstacles such as connectors), the work by the aseptic connecting means is easy.

[Advantages and effects of the device] After centrifuging, all unnecessary plasma (upper layer) and red blood cells (lower layer) are discharged, and then only the buffy coat (middle layer) containing bone marrow is intensively separated, and only the highly viscous bone marrow is extracted. Since it can be quickly discharged from the lower part of the main body by spontaneous falling, the separation operation of the bone marrow fluid can be efficiently performed in a short time.

It is an excellent device that has the effects of the above.

[Brief description of drawings]

FIG. 1 is a schematic view of a bone marrow transplant bag of the present invention, FIG. 2 is a usage state diagram of the bone marrow transplant bag, FIG. 3 is a schematic view of a conventional bone marrow transplant bag, and FIG. 4 is a conventional bone marrow transplant. It is a usage condition figure of a bag for a car. In the figure, 1 is a bone marrow transplant bag, 2 is a main body, 3 is a bone marrow introduction tube, and 4 is a bone marrow discharge tube.

Claims (1)

[Scope of utility model registration request] 1. A flexible bag-shaped main body is provided with a bone marrow introduction tube at the upper end, and a bone marrow discharge tube is provided at the lower end of the main body.
A bag for bone marrow transplantation, wherein the bottom of the main body is formed in a funnel shape.