JPH0889558A - Blood component separation method - Google Patents

Abstract

(57) [Summary] [Purpose] A blood component separation method for obtaining plasma and concentrated red blood cells that do not contain white blood cells. [Structure] Using a synthetic resin blood bag set consisting of a parent bag 1, a plasma collection child bag 4, and a concentrated red blood cell collection child bag 5, blood is collected in the parent bag 1 and centrifuged, and then the parent bag 1 Two side-by-side moving hands 17,
Squeeze at 18, then move the moving hands 17, 18 toward the peripheral portion of the parent bag body while separating them so as to face each other to form a passage for collecting concentrated red blood cells in the parent bag 1, and finally in the parent bag 1. The blood component separation method in which each layer of the plasma portion / concentrated red blood cell portion is transferred to the respective child bags 4 and 5 by applying pressure to the blood, and only the buffy coat 16 remains in the parent bag 1.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for separating each blood component in whole blood using a blood bag.

[0002]

2. Description of the Related Art A blood bag is used for taking out various blood components contained in whole blood and formulating them. As such a blood bag, there is a blood bag set disclosed in Japanese Patent Publication No. 63-20144. This is equipped with a plasma collection connection at the top of the parent bag where whole blood is collected.
The lower end portion of the parent bag is provided with a concentrated red blood cell collection connection portion, and the plasma collection child bag and the concentrated red blood cell collection child bag are connected to the upper connection portion and the lower connection portion through the upper tube and the lower tube, respectively. There is.

When separating the whole blood collected in the parent bag into blood components, the whole blood bag set is placed in a centrifuge and the whole blood in the parent bag is centrifuged. As a result of the centrifugation, a plasma layer is formed in the upper part inside the parent bag, a thin layer containing white blood cells (buffy coat) is formed in the middle part, and a layer containing red blood cells is formed in the lower part. After that, the parent bag is squeezed by a blood separation squeezing device, so that the blood components in the upper and lower parts of the inside of the parent bag pass through the connecting parts and the respective tubes, and the blood plasma collecting child bag and the concentrated red blood cell collecting child. Simultaneously transferred to the bag.

The above blood bag set can transfer the blood components in the upper layer and the lower layer inside the parent bag to two child bags at the same time, which is very advantageous in terms of labor saving and shortening of working time. By the way, when centrifuging a blood bag set with a centrifuge, the blood bag set is set in the centrifuge so that the tube does not come into contact with the body of each bag.

However, since the above blood bag set is provided with a connecting portion at the lower end and a tube is connected to this connecting portion, when the blood bag set is set in the centrifuge, the tube at the lower end of the bag is inevitable. It is easy to set while touching the top and bottom sides of the parent bag body.

[0006] When set in this way, the side surface of the parent bag is strongly pressed against the tube outside when centrifugally separated, and the periphery of the pressed portion of the parent bag is swollen and a protrusion is easily formed. In particular, the lower part of the main body of the parent bag is subject to a large gravitational acceleration during centrifugation, so that a protrusion is likely to be formed.

When such a protrusion is formed, there is a problem that the protrusion formed on the parent bag is easily damaged. Therefore, in order to solve this problem, a blood bag set as shown in FIG. 3 was developed. The blood bag set of FIG. 3 has a parent bag 1, a blood collection needle 3 with a blood collection tube 2, a child bag 4 for collecting plasma, a child bag 5 for collecting concentrated red blood cells, and tubes 6 and 7 attached above and below the parent bag.

The parent bag 1 is for collecting whole blood, and is mainly composed of a main body 8. The main body 8 is composed of two sheets made of synthetic resin, and the periphery of these sheets is adhered to form a bag. There is. Blood sampling tube 2 on the upper end of the main body 8
, A blood plasma transfer connection 10, a concentrated red blood cell transfer connection 11, and a blood transfusion port 13 with a pair of protectors 12.

The plasma transfer connection 10 and the concentrated red blood cell transfer connection 11 are sandwiched and fixed between the two sheets of the main body 8 when the peripheral edges of the two sheets are bonded. The plasma transfer connection part 10 and the concentrated red blood cell transfer connection part 11 are provided with communication pieces 14 and 15 for connecting the inside and outside of the main body 8 by bending. The lower end of the plasma transfer connection 10 is opened at the upper end inside the main body 8, and the lower end of the concentrated red blood cell transfer connection 11 is opened at the lower end inside the main body 8 while being at the lower end inside the main body 8. Opposed through a small gap.

The plasma collection child bag 4 and the concentrated red blood cell collection child bag 5 are connected to the plasma transfer connecting portion 10 of the main body 8 so that the respective blood components in the upper and lower parts of the main body 8 can be transferred.
・ The tubes 6 and 6 are connected to the connection part 11 for transferring concentrated red blood cells.
It is connected via 7.

When using the blood bag set as described above, first, the blood collecting tube 3 and the connecting portion 9 are connected from the blood collecting needle 3.
The whole blood is introduced from the donor into the main body 8 of the parent bag 1 via the.

Then, the blood bag set is placed in a centrifuge, and the blood in the main body 8 is centrifuged to separate the main body 8
An upper layer containing plasma is separately formed in the upper part of the inside, a buffy coat layer is formed in the middle part, and a lower layer containing red blood cells is separately formed in the lower part.

After centrifuging the whole blood as described above, the end of the blood collection tube 2 on the side of the connecting portion 9 is shut off, and then the communication pieces 14 and 15 are bent and bent, and the inside of the main body of the parent bag 1 is
Via the connecting portions 10 and 11 and the tubes 6 and 7, the blood plasma collection child bag 4 and the concentrated red blood cell collection child bag 5 are communicated with each other, and at the same time, plasma and concentrated red blood cells are transferred.

[0014]

When the above blood bag set is used, the lower end portion of the concentrated red blood cell transfer connection portion 11 opens at the lower end portion inside the main body 8 and a small gap is formed between the lower end portion inside the main body 8 and the inside. Since they face each other, blood enters the connecting portion 11 when blood is collected in the parent bag body 8. When the blood in the connection portion 11 is centrifuged in that state, the connection portion 1
In 1, it is separated into each layer of plasma, buffy coat and concentrated red blood cells. The fact that plasma / buffy coat is contained in the connecting portion 11 means that when the concentrated red blood cells are transferred to the child bag, highly concentrated concentrated red blood cells cannot be obtained. The present invention has been made for the purpose of collecting blood components of highly pure plasma and concentrated red blood cells, as compared with the case of using the conventional method.

[0015]

According to the present invention, a blood collecting needle is connected by a tube to each position opened at the upper end of a parent bag, and a plasma collecting child bag and a concentrated red blood cell collecting child bag are provided. Using a blood bag set made of synthetic resin, each of which is connected by a tube containing a communication piece that is connected by bending and bending, (1) Blood is collected in the parent bag and centrifuged to collect plasma, buffy coat, and concentrate Operation to divide into layers of red blood cell part, (2) Two side-by-side moving hands arranged from the upper end of the parent bag toward the concentrated red blood cell collection child bag toward the lower end of the parent bag Operation of squeezing from the outside of the parent bag with (3) pushing the two moving hands toward the peripheral portion of the parent bag body while facing them apart from each other, and between the two moving hands, and Red blood cells in the parent bag Operation to form a take-up passage, (4)
After connecting the inside of the parent bag and each child bag, by pressing the blood in the parent bag, each layer of the plasma part and concentrated red blood cell part is transferred to each child bag, leaving only the buffy coat in the parent bag. The gist is a method for separating blood components, which is characterized by comprising an operation.

[0016]

According to the present invention, since the blood bag set is made of synthetic resin, the blood component separated into each layer inside the parent bag can be distributed to each child bag only by devising the squeezing condition outside the parent bag. It can be moved.

The blood bag set adopted in the present invention is
Blood collecting needles are connected by tubes to respective portions of the connecting portion opened at the upper end of the parent bag, and further, a blood plasma collecting child bag and a concentrated red blood cell collecting child bag are connected in a tube-communicable state. The state of being able to communicate here means that the inside and outside of the parent bag is bent by bending the inside of the connecting portion, that is,
That is, a communication piece (also called a bent bar) that connects the inside of the parent bag and the inside of the child bag is provided. Such blood bag sets are well known. In the present invention, it is also a well-known operation to divide the blood collected in the parent bag into layers of a plasma portion, a buffy coat and a concentrated red blood cell portion by centrifugation.

In the present invention, the second operation (2) is performed.
The operation of squeezing from the outside of the parent bag with the hands arranged side by side in the book is to keep as little blood as possible in the area of the bag body being squeezed. From the point where the parent bag is connected to the child bag for collecting concentrated red blood cells to the vicinity of the lower end of the parent bag, a moving hand is provided so that when the blood components are transferred, the passage of the concentrated red blood cells. This is because

In the present invention, there is a third (3) operation for forming a passage for collecting concentrated red blood cells in the parent bag.
The moving hand of the book is pushed toward the peripheral portion of the parent bag body. It is preferable that the passage has a cross-sectional area equal to or slightly larger than the inner area of the communication tube. The reason for pushing in the movement of the moving hand is to prevent buffy coat or plasma from entering the passage. In addition, the lower end of the moving hand must extend below the buffy coat layer so that the concentrated red blood cells are not mixed with buffy coat or plasma when the blood components are transferred. For these operations, it is preferable that the moving hand is rod-shaped and is in surface contact with the bag body.

In the present invention, there is a fourth operation (4) for transferring the blood component to the child bag. In order to transfer each blood component even if the blood in the parent bag is pressed, the communication piece in the communication tube is bent and folded to previously connect the inside of the parent bag with the inside of each child bag. Also at this time, the bag body portion at the place where the moving hand is present remains pressed.

In the present invention, the plasma portion from the parent bag is
When each layer of the concentrated red blood cell portion is transferred to each child bag and only the buffy coat remains in the parent bag, it may be visually controlled or optically controlled by a photo sensor or the like.

When the conventional blood bag set is put on a centrifugal separator, the main body of each bag and the tube are easily contacted with each other and the parent bag is easily damaged. The parent bag is less likely to be damaged as all tubes are connected to the top of the bag.

[0023]

Embodiments of the present invention will now be described in detail with reference to the drawings. FIG. 1 is a plan view of a blood bag set adopted in the present invention, and FIG. 2 is a plan view of a blood bag set for explaining the method of the present invention. The blood bag set of FIG. 1 has a parent bag 1, a blood collection tube 2 with a blood collection needle 3, a child bag 4 for collecting plasma, a child bag 5 for collecting concentrated red blood cells, and tubes 6 and 7 attached to the parent bag.

The parent bag 1 is for collecting whole blood, and is mainly composed of a main body 8. The main body 8 is composed of two sheets of synthetic resin such as polyvinyl chloride, and the periphery of these sheets is heated. The bag is fused and bonded. A connecting portion 9 to which the blood sampling tube 2 is connected to the upper end portion of the main body 8, a plasma transferring connecting portion 10, and a concentrated red blood cell transferring connecting portion 11,
A blood transfusion port 13 with a pair of protectors 12 is provided.

The plasma transfer connection part 10 and the concentrated red blood cell transfer connection part 11 are made of soft tubes made of synthetic resin such as polyvinyl chloride, and are used to bond the two sheets of the main body 8 together at their peripheral edges. It is fixed by being sandwiched between the sheets.
The connecting parts 14 and 15 for connecting the inside of the main body 8 and the outside, that is, the inside of the main body 8 and the inside of the child bags 4 and 5 by bending the inside of the plasma transfer connection 10 and the concentrated red blood cell transfer connection 11 are provided. ing. Both the lower end of the plasma transfer connection 10 and the lower end of the concentrated red blood cell transfer connection 11 are open at the upper end inside the main body 8.

A blood preservation solution such as a CPD solution or an ACD-A solution is enclosed in the main body 8, and the concentrated red blood cell sampling bag 5 mainly contains mannitol and adenine which can extend the effective period of red blood cells to 42 days. A red blood cell preservation solution as a component, so-called MAP solution, etc. is enclosed.

The plasma collection child bag 4 and the concentrated red blood cell collection child bag 5 are connected to the plasma transfer connecting portion 10 of the main body 8 so that the blood components in the upper and lower parts inside the main body 8 can be transferred after centrifugation. -Connected to the concentrated red blood cell transfer connection portion 11 via tubes 6 and 7, respectively.

When using the above blood bag set, first, the blood collection tube 3 and the connecting portion 9 are connected from the blood collection needle 3.
The whole blood is introduced from the donor into the main body 8 of the parent bag 1 via the. Then, the blood bag set is put into a centrifuge, and the blood inside the main body 8 is centrifuged at about 4000 rpm, and the upper layer containing plasma is in the upper part of the main body 8 and the buffy coat 16 is in the lower part. The lower layers containing red blood cells are formed separately.

After centrifuging whole blood as described above, FIG.
As shown in the figure, with the moving hands 17, 18 aligned, the parent bag 1
The outer hands of the parent bag 1 are squeezed from the outside of the parent bag 1 from the location connected to the concentrated red blood cell collecting child bag 5 at the upper end portion thereof toward the lower end portion of the parent bag 1. As a result, the two films forming the main body 8 are pressure-bonded only at that position. Then, the two moving hands 17 and 18 are pushed toward the peripheral portion of the parent bag body while being separated so that the two move in parallel. In FIG. 2, the moving hand 17 is pushed leftward in the drawing and the moving hand 18 is pushed rightward in the drawing. The lower end of the concentrated red blood cell collection passage (not shown) in the parent bag thus formed is located below the buffy coat layer 16.

The connecting pieces 14 and 15 are folded to form the connecting portion 1.
By connecting the inside of the parent bag 1 and the inside of each of the child bags 4 and 5 through the tubes 0 and 11 and the tubes 6 and 7, the blood in the parent bag 1 is pressed to move the layer of the concentrated red blood cell portion to the moving hand 1.
The lower part of the concentrated red blood cell collecting passage formed by 7, 18 is passed to the child bag 5, and at the same time, the layer of the plasma portion is also transferred to the child bag 4. Then, only the buffy coat 16 is left in the parent bag 1. When it is desired to enlarge the passage for collecting concentrated red blood cells, the passage portions of the two films forming the main body 8 must be loosened, and the distance between the moving hands 17 and 18 facing each other needs to be slightly closer before the transfer of each blood component. There is.

As a result of separating blood components by the method of the present invention,
It was possible to transfer 93% of the red blood cells in the parent bag 1 to the child bag 5 for collecting concentrated red blood cells, and leave 80% of the white blood cells existing in the parent bag 1 in the parent bag 1.

As a comparative example, as a result of centrifuging blood components by the method shown in FIG. 3, 88 of red blood cells in the parent bag 1 were found.
Only 65% of the white blood cells in the parent bag 1 can be transferred to the concentrated red blood cell collection child bag 5, and 65
Only% left in parent bag 1.

[0033]

As described above, the preparation of concentrated red blood cells and plasma containing few white blood cells can be obtained by separating blood components according to the present invention.

[Brief description of drawings]

FIG. 1 is a plan view of a blood bag set used in the present invention.

FIG. 2 is a plan view of a blood bag set for explaining the method of the present invention.

FIG. 3 is a plan view of a conventional blood bag set.

[Explanation of symbols]

 1 Parent Bag 2 Blood Collection Tube 3 Blood Collection Needle 4 Child Bag for Plasma Collection 5 Child Bag for Concentrated Red Blood Cell Collection 6 Tube 7 Tube 8 Body 9 Connection Portion 10 Plasma Transfer Connection Portion 11 Concentrated Red Blood Cell Transfer Connection Portion 12 Protector 13 Blood Transfusion Port 14 Communication piece 15 Communication piece 16 Buffy coat 17 Moving hand 18 Moving hand

Claims (1)

[Claims] 1. A communication in which a blood collection needle is connected to each of the openings at the upper end of the parent bag by a tube, and the plasma collection child bag and the concentrated red blood cell collection child bag are connected by bending them. Using a blood bag set made of synthetic resin, each connected by a tube containing a piece,
(1) An operation of collecting blood in a parent bag and separating it into layers of a plasma portion, a buffy coat, and a concentrated red blood cell portion by centrifugation; Operation from the outside of the parent bag toward the lower end of the parent bag from where it is located, (3) Push the two moving hands toward the peripheral edge of the parent bag body. An operation of moving and separating them so as to face each other and forming a passage for collecting concentrated red blood cells in the parent bag between the two moving hands, and (4) communicating the inside of the parent bag with the inside of each child bag Therefore, the blood component in the parent bag is pressed to transfer each layer of the plasma portion and the concentrated red blood cell portion to each child bag, and only the buffy coat remains in the parent bag. Method.