JPH09103252A - Fermented feed for eliminating and neutralizing malodor of excreta - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a safe fermented feed for eliminating and neutralizing a malodor of excreta having excellent malodor eliminating and neutralizing actions on the excreta. SOLUTION: This fermented feed for eliminating and neutralizing a malodor of excreta comprises a component liquid containing a tree prepared froth a Japanese cedar, a Japanese cypress and/or a pine (at about 6-7wt.% concentration, usually in an amount of 5-15 pts.wt. based on 100 pts.wt. fermented liquid) in the fermented liquid (about 6wt.%) produced by inoculating Bacillus subtilis (Bacillus natto) in a liquid culture medium containing rice brans, soybeans, a carbon source and water, culturing the Bacillus subtilis at pH 7.5-10 (especially pH about 9) and about 40-45 deg.C temperature with aeration and filtering the resultant culture liquid. The feed is eaten to provide malodor eliminating and neutralizing effects on feces and urine not only for pets such as a cat but also for livestock animals of chickens and pigs.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to a fermented feed for excrement and deodorization of manure, which has not only a deodorizing action of urine but also a deodorizing action of feces. INDUSTRIAL APPLICABILITY The present invention is widely used in the field of preventing generation of offensive odor of manure by feeding animals, particularly pets (cats, dogs, rabbits, hamsters, etc.) or domestic animals (especially chickens, pigs, etc.).

[0002]

2. Description of the Related Art Heretofore, as a method of preventing bad smell for pets such as cats, a so-called cat litter or a spray type has been used. Urine odor eliminating agents containing phytic acid and salts thereof as active ingredients are also known (Japanese Patent Application Laid-Open (JP-A) No)
195860, JP-A-1-275522).

[0003]

However, the cat sand is intended to adsorb and remove the malodor of the excrement, and does not prevent the generation of the malodor of the excrement itself. Therefore, if the stool is not stooled on the sand, the malodor cannot be removed, and since it is sandy, powdering tends to occur, which is a problem in handling. In addition, the spray type is also the same as cat sand in that it requires the trouble of spraying and does not prevent the generation of offensive odor in the excrement itself. Further, although the latter deodorant is an edible substance, it eliminates urine odor or body odor and does not eliminate fecal odor, and it is not the fermented product itself that is fermented, and therefore has a biological balance. In some cases, safety may not be sufficient.

It is an object of the present invention to provide a fermented feed for excrement and deodorization of manure, which is safe and has excellent excrement and deodorization of excrement by using rice bran and soybeans as raw materials.

[0005]

Means for Solving the Problems The present inventors have conducted intensive studies on whether there is a natural fermented product that can prevent the odor of manure when eaten by a cat or the like. The present inventors have found that the purpose can be achieved by adding a predetermined additive to the obtained fermentation liquor, and have completed the present invention. Fermentation feed for deodorizing manure of the present invention, rice bran, soybeans, inoculation of Bacillus subtilis into a liquid medium containing a carbon source and water, cultivated, the fermentation broth produced by filtration, cedar, cypress and pine It is characterized in that it contains a tree-containing component liquid made from at least one of the above.

The above-mentioned "rice bran" refers to rice germ, defatted rice germ, rice bran, defatted rice bran, and the like, and "soybeans" refers to defatted soybean, china flour, soy flour, soybean scum, and a hydrolyzate thereof. Etc. These are added in an amount of 10 to 500 parts by weight of soybeans (particularly 1 part by weight) when rice bran is 100 parts by weight.
It is preferably 0 to 20 parts by weight). This is because if the amount is less than 10 parts by weight, the amount of peptides will be too small.
This is because if the amount exceeds 500 parts by weight, the decomposition rate of the soybeans decreases. As the above-mentioned “carbon source”, a commonly used one (eg, one or more of glucose, dextrin, lactose, starch and the like) can be used.

[0007] The medium used in the present invention is a "liquid medium" containing water. In this medium, usually, a phosphorus compound such as phytic acid and phosphate is further used. As the phytic acid, a sodium salt or a calcium salt is preferable. The fermentation conditions are usually pH 7.5 to 1
0 (especially around 9), and the culture temperature is about 40 to 45 ° C. The reason why alkaline culture is preferable is that Bacillus subtilis has a high activity under weakly alkaline conditions and the growth of various bacteria is small. To make this alkaline, sodium bicarbonate or the like is used as an alkaline agent.

Further, in this liquid medium, rice bran, soybeans, a carbon source and water are mixed with the rice bran,
The concentration of soybeans and carbon source is preferably 5 to 15% by weight.
If it is less than 5% by weight, it becomes too thin and the production efficiency is lowered, while if it exceeds 15% by weight, the aeration and stirring efficiency is lowered, which is not preferable. Since it is a liquid culture, it can be cultured by aeration and stirring. In addition, protease can be used as a medium material. This case is useful because it decomposes soybean peptides. The amount of the alkaline protease added is usually 0.001 to 1% by weight. This is because if it is less than 0.001% by weight, the decomposition efficiency decreases, while if it exceeds 1% by weight, the decomposition efficiency does not change so much and the cost increases. The above “fermentation liquid” may be a liquid obtained by filtering the cultured fermentation liquid, a liquid obtained by performing post-treatment such as decolorization, or a concentrated liquid obtained by concentrating the liquid, distillation, or the like. May be used.

The above-mentioned "tree-containing component liquid" includes cedar, cypress and / or
Or a liquid substance obtained by carbonization, steam distillation, or solvent extraction from a plant containing pine. In particular, conifers are preferred. The tree-containing component liquid may be a liquid obtained as a result of the treatment, a concentrated liquid thereof, or a liquid diluted with water or the like. This tree-containing component solution contains about 6 to 7% by weight of an organic component, that is, a water content of 9%.
In the case of 3 to 94% by weight, about 5 to 15 parts by weight is preferably added to 100 parts by weight of the fermentation liquid (concentration of evaporation residue; 6% by weight). This is because if it is less than 5 parts by weight, the effect may not be sufficient, and if it exceeds 15 parts by weight, the effect tends to be saturated. In terms of the weight ratio of the residue, 1 part by weight of the evaporation residue in the fermentation liquor is added to the evaporation residue in the tree-containing component solution of 0.05 to
It is about 0.2 parts by weight.

[0010]

DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, the present invention will be described specifically with reference to examples. Example 1 (1) Production of Fermentation Liquid and Preparation of Fermented Food (Test Product) First, a fermentation liquid is produced by the method shown below. That is,
Defatted rice bran; 30 kg, soybean meal (or defatted soybean); 3 k
g, bittern; 5 kg, water; 500 kg. Note that p
H is around 9. The medium is sterilized at 121 ° C. for 30 minutes, then cooled, then inoculated with 0.05 kg of Bacillus natto (manufacturer: Naruse Fermentation Chemistry Laboratory) and aerated and stirred at 40 to 45 ° C. for about 48 hours. And cultured.

[0011] Thereafter, the culture was squeezed and filtered, treated with activated carbon and perlite, deodorized and decolorized to obtain a fermentation broth. Various types of activated carbon such as powdered activated carbon (activated carbon S, activated carbon K, etc.) and granular activated carbon (activated carbon SG, etc.) can be used as the activated carbon. As the pearlite, "Perlite No. 4180" (Dikarain Orient Co., Ltd.) Made)
It was used. This fermentation liquid is a colorless or pale yellow transparent liquid, has a fermentation flavor, and has a water content of 94% (by the atmospheric pressure heating and drying method). It detects general viable bacteria, Escherichia coli, mold, yeast, arsenic, and heavy metals. Was not done.

As a test product of the fermented food, 10 parts by weight of the tree-containing component liquid (concentration of evaporation residue; 100% by weight of the fermentation liquid (concentration of evaporation residue; 6.0% by weight)) was used.
(6.5 wt%) was used. This is
0.11 part by weight of the evaporation residue in the tree-containing component liquid is shown with respect to 1 part by weight of the evaporation residue in the fermentation liquid. This tree-containing component liquid is made up of cedar, cypress, and pine chips of approximately equal volume.
Dry distillation at ~ 180 ° C for several tens hours, then 100 ~ 12
It is obtained by distilling (at normal pressure or reduced pressure) at about 0 ° C. to mainly remove tar components and, if necessary, diluting with water. In addition, the essential oil obtained by steam distillation can also be used. The pH of the tree-containing component solution was 6 to 7, the color was colorless, pale yellow or pale brown, and had a slight flavor. The component analysis is as follows. The analysis method was based on the commentary on the hygiene test method, soil test method JIS K0102 and high frequency plasma emission spectroscopy.

[Composition component results (unit: weight%)] Water content: 93.1%, ethanol: 0.4%, organic matter (loss on ignition) 6.4%, inorganic matter (ash): 0.1%, sulfuric acid Ion: 16 mg / l, conductivity: 430 μs / cm, p
H; 4.1 (20 ° C.), nitrogen; 37 mg / l, phosphorus;
Less than 0.5 mg / l, sodium; 12 mg / l, calcium; 15 mg / l, iron; 0.18 mg / l, magnesium; 1.9 mg / l, silicon; 6.9 mg / l, potassium; 8.3 mg / l , Copper; 0.01 mg / l, cadmium, lead and arsenic were not detected, total phosphorus amount: 720 mg / 1
00g or more (800-900).

(2) Performance Evaluation (A) Urine Deodorization Test First, the urine deodorization test was performed under the following conditions, and the results are shown in Tables 1 to 9. [Test conditions] Feeding method: 100 g of a cat dry food containing 0.5% of the above test product was added to each of the test cats (No. 1 to 10) shown in Table 1 (dispensed twice). , Drinking water was constantly supplied. Table 2 shows the amount of feed and the amount of drinking water for each cat. Urine test method; Tables 1 to 6 (spontaneous urine, test period up to 21 days), Tables 7 to 9 (urine collected by catheterization, test period up to 30 days) Odor evaluation; by human It was based on a sensory test. 0 in the table:
Deodorize. 1: Odor is recognized. 2: Strong odor is recognized. 3: Odor and ammonia odor are recognized.

[0015]

[Table 1]

[0016]

[Table 2]

[0017]

[Table 3]

[0018]

[Table 4]

[0019]

[Table 5]

[0020]

[Table 6]

[0021]

[Table 7]

[0022]

[Table 8]

[0023]

[Table 9]

From the above results, according to the results of Tables 1 to 6, an odor "1" was recognized before feeding, and in many cases, when the odor was left for 6 hours, the odor became "2 to 3" (Table 3). . However, after 5 days from the feeding, the odor after standing for 6 hours decreases to almost “1” (Table 4). Seven days after feeding, the odor could be reduced to almost “0” immediately after urination (Table 5),
After 21 days, almost no odor was recognized even after 6 hours of urination "0" (Table 6). Further, according to the results of Tables 7 to 9, in the case of odor "1" before the feeding (Table 7), almost 15 days after the feeding, almost no odor was observed immediately after urination (Table 8), and after 30 days from the same day Showed almost no odor even after standing for 6 hours (Table 9). From the above, it is shown that this test product has an excellent deodorizing effect on urine. In particular, it is very practical because it is effective not only immediately after urination but also after leaving for a certain period of time.

(B) Fecal Deodorization Test In the test using the odor sensor, the odor molecule causes two changes in the frequency of adsorption on the synthetic bimolecular coating on the quartz oscillator, and the change in the frequency is measured. The measurement was carried out using a measuring instrument expressed as odor intensity. As a test method, 2 m / day for adult male cats
1 of the above test sample was provided, and an odor sensor and a computer were connected to measure the deodorizing effect of urine odor and fecal odor. The measuring method was based on the “atmospheric standard gas phase method”. The result is shown in FIG. In this figure, the odor value is 1 as the odor value at the time 0 (before the test)
00 and shown as a relative value of this value. Line A is the result of deodorization of urine of this test product, line B is the result of deodorization of feces of this test product, and line C is a comparative product. The results of the deodorization of feces (when included) are shown.

According to these results (FIG. 1), the fecal odor is significantly reduced after 3 days, and is more effective than urine odor. Although the comparative product containing no tree-containing component liquid also showed a slight fecal odor eliminating effect, the effect was less than that of the product containing the tree-containing component liquid. Therefore, it can be seen that the tree-containing component liquid added to the fermentation liquid greatly contributes to the deodorizing effect of feces. In addition, as described above, this fermented food is free from harmful bacteria and heavy metals, and is a natural fermented product made from completely natural raw materials. Therefore, it uses rice bran and soy products that are safe and are originally useful as healthy feed. Therefore, it contains useful nitrogen compounds (such as amino acids), ash, phosphorus compounds, etc., and therefore has a very good balance for health.

(C) Urinary stone-preventing effect A predetermined no. For cats, the urinary stones in the natural urine were evaluated by the usual urinary stone inspection method in cow urine (ammonia addition method, the number of urinary stones in the visual field under a microscope observation of about 400 times). The results are shown in Table 10. According to this result, although there are some variations, urinary stones are continuously discharged into the urine after feeding this test product, and the increase of urinary stones and the closure of the ureter are prevented. Is shown. It is presumed that since this test product contains a relatively large amount of phosphorus component, it acts on urolith to prevent its hypertrophy and promote its discharge.

[0028]

[Table 10]

Example 2 In this example, the deodorizing effect and the growth effect of manure on broiler chicks were examined. Rearing conditions are as follows. (1) Feed; Test feed A in this example is a self-mixed feed shown below. Test feed A: 100 parts by weight of “Brohit” (trade name, manufactured by Kyodo Feed Co., Ltd.) was added with the test product used in Example 1 in an amount of 0.1%.
5 parts by weight were blended. The “Blohit” (Kyodo Feed Co., Ltd.) consists of 50 pellets (Kyodo Feed Co., Ltd.) and 50 mashes (Kyodo Feed Co., Ltd.).
It is blended in parts by weight. As the comparative feed A, only "Blohit" (manufactured by Kyodo Feed Co., Ltd.) was used, and no test product was blended.

(2) Breeding method; leveling in a windless poultry house (bred on a flat ground) (3) Object: 500 chicks (broilers) (average body weight 44.5 g) (4) Feeding method; Automatic feeding (use automatic feeding machine and automatic watering machine) (5) Smell test; sensory test by humans Under the same conditions using both feeds, manure in a poultry house using test feed A under comparative breeding The odor was reduced 3 to 7 days after rearing. On the other hand, when the comparative feed A was used, the smell was the same as at the beginning, and did not decrease even after 7 days.

Example 3 In this example, the deodorizing effect and the growth effect of manure on adult broiler chickens were examined. The breeding conditions are the same as in Example 2 except for the following. (1) Object: adult chicken (broiler) (test plot; 30 birds)
(2) Evaluation items: Deodorization of manure, stool (feces) hardness, number of eggs laid, egg production rate and egg weight (3) Test period: Up to 8 weeks after ingestion It is shown in Table 11.

[0032]

[Table 11]

According to the results, in the test plot, the odor almost disappeared two weeks after the breeding started, and the odor disappeared after eight weeks. However, the control group had the same smell even after 8 weeks. In addition, the evaluation of the number of eggs laid, the rate of egg production and the weight of eggs were 109%, 108% and 10% after 2 weeks, respectively, as compared with the control group.
8%, 119%, 119% and 120% after 6 weeks,
After two weeks, they were 109%, 109% and 110%. As described above, when the test sample feed of the present invention was used, the smell of manure was significantly reduced, and the growth of chickens could be improved by 10 to 20%.

Example 4 In this example, the deodorizing effect of manure on pigs was examined. Rearing conditions are as follows. (1) Feed; Test feed B in this example is as follows. Test feed B: "Live meat pork 7"
8 "(manufactured by Taiyo Feed Co., Ltd.) in 100 parts by weight of Example 2
In the same manner, 0.5 part by weight of the test product used in the above was blended. This "Live meat pork 78" (manufactured by Taiyo Feed Co., Ltd.)
Is a cereal [corn, milo, (barley)] 76%,
Vegetable oil cake [soy oil cake, rapeseed oil cake (corn jam meal)] 16%, animal and animal feed [fish meal, (meat bone meal)] 3%, alga (corn gluten feed) 2%, other [animals Oils and fats, calcium carbonate,
[Calcium phosphate, salt, vegetable oil, corn steep liquor] 3%. As the comparative feed B, only "Live meat pork 78" (manufactured by Taiyo Feed Co., Ltd.) was used, and no test product was used.

(2) Object: piglets and pigs 4 to 5 months after birth; Land York Derek (LWD), initial weight: 70 to 75 kg (3) Rearing method: 1 pig house (1 group) 5) reared (stall breeding). That is, 5 animals were bred in each of the test group and the control group. (4) Test period; 14 weeks (5) Feeding method; semi-conveyance feeding (feed twice daily, almost at regular time) (6) Collection method of test manure; In test section, for test section or control It is 10 at random from each pig bun (5 per group) for ward
Extract from each place (about 100g per place), extract manure properly from the place, mix it well, and put in a beaker 30g
It was measured and taken as one sample (test sample).

(7) Odor test; t-
A comparative sensory test was conducted in which 10 samples from 1 to t-10 were prepared, 1 sample was prepared in the control group, and the smell of the manure in the beaker was compared with the control group sample. For the judgment method, a human sensory test was used, and the degree
The evaluation was also performed by classifying the results into scores and assigning a score to each of the scores to make a numerical value (the total score of each evaluation at t-1 to 10 places is defined as a total score).

The evaluation is as follows. That is, as compared with the control group, a: the deodorizing effect is large (evaluation +2),
b: Slightly deodorizing effect is observed (Evaluation +1), c: No change (Not much change) (Evaluation ± 0), d: Slightly strong urine smell (Evaluation -1), e: Strongly smell urine (Evaluation -1) -2). Under the above conditions, the test was performed for each of the test group and the control group, and the results were shown in Table 12 and FIG.
Shown in

[0038]

[Table 12]

According to the results, as shown in FIG. 2, particularly in the test group, the odor of manure was improved over time in the test group as compared with the control group. In particular, after the twelfth week, almost all samples are evaluated as a
It was evaluated, and the bad smell was remarkably improved.

Example 5 In this example, the deodorizing effect and the growth effect of manure on pigs were examined. The breeding conditions are the same as in Example 4 except for the following. (1) Feed; "Live meat pork 78" (manufactured by Taiyo Shoku Co., Ltd.) used in Example 4 was used as a comparative feed used as a test feed in this example. The administration of the test article is such that 0.5 to 0.7 parts by weight is continuously administered to 100 parts by weight of the comparative feed.

(2) Evaluation items: deodorization of feces and urine, feces (feces)
Breeding period and slaughter quality up to 110 kg to 115 kg (only meat is about 73 to 75 kg)] (3) Test period; up to 13 weeks after ingestion Under the above conditions, test group and control group Each test was performed, and the results are shown in Table 13.

[0042]

[Table 13]

According to this result, in the test plot, the fourth
The effect of deodorization appeared from the week, and the effect became remarkably large in the 8th to 11th weeks. In addition, the breeding period until the body weight can be shipped is 13 weeks (about 3.
3 months), but in the test plot, it was sufficient at the 11th week (2.8 months). Therefore, the breeding period that can be shipped is about 20
%, And its economic efficiency is extremely large. This seems to be due to the good bite of the bait and the good growth efficiency. In addition, when the pigs were slaughtered at the time of shipment and their slaughtered meat quality was compared, in the test plot, the lipids were whitish and of good quality, the flesh color was light and pale, the flesh was good, and the crotch was good. I was stretching. It should be noted that the present invention is not limited to the specific embodiments described above, but may be variously modified within the scope of the present invention in accordance with the purpose and application.

[0044]

INDUSTRIAL APPLICABILITY The fermented feed for deodorizing manure and urine according to the present invention has an excellent deodorizing effect not only on urine odor but also on fecal odor.
Therefore, even if a pet such as a cat is bred indoors, the room will not be filled with a bad odor, and since it has a deodorizing effect by eating, it is possible to prevent the bad odor of feces and urine itself from occurring, and it is possible to prevent the cat from using the conventional method. There is no need to stool etc. in a predetermined fixed place, and since it is a liquid substance, there is no problem of dusting like cat sand. Further, as described above, urine stones have an effect of being excreted in urine, so that it is possible to prevent cats and the like from being impaired in health due to urine stones and the like (furthermore, urological syndrome). Furthermore, by using the fermented feed for excrement and deodorization of feces of the present invention, the excrement of feces and urine of chickens or pigs is eliminated, and complaints of bad odors from the neighborhood are also eliminated, and further, the growth effect of those livestock animals (of chickens) Improvement of egg laying rate, weight gain of pigs and improvement of meat quality) are also improved. Therefore, the feed of the present invention is extremely useful.

Since this fermented feed is a natural fermented product made of completely natural raw materials, harmful bacteria and heavy metals are not recognized,
Since it is safe and originally made from rice bran and soybean products that are originally useful as a healthy feed, and contains useful nitrogen compounds (amino acids, etc.), ash, and phosphorus compounds, it is a very well-balanced healthy feed. is there. Further, in the present fermented feed, since the solid matter is removed using the fermentation liquid, the active ingredient concentration is high and the quality thereof is excellent.
It can be easily mixed with pet food, and its appearance is the same as conventional pet food.

[Brief description of the drawings]

FIG. 1 is a graph showing the results of a deodorizing test of manure in Example 1.

FIG. 2 is a graph showing the results of a deodorizing test of manure in Example 4.

Claims (3)

[Claims] 1. A fermentation broth produced by inoculating a liquid medium containing rice bran, soybeans, a carbon source and water with Bacillus subtilis, culturing and filtering the mixture, and then producing a fermented liquor from which at least one of cedar, cypress and pine is used as a raw material. A fermented feed for deodorizing manure, which is characterized by containing a tree-containing component liquid produced as described above. 2. The fermented feed for deodorizing manure according to claim 1, which is used for cats, chickens or pigs. 3. The evaporation residue in the tree-containing component liquid is
With respect to 1 part by weight of the evaporation residue in the fermentation broth, 0.05
The fermented feed for deodorizing manure according to claim 1 or 2, which is ˜0.2 parts by weight.