JPH09169616A - Anti-aging cosmetics - Google Patents

Abstract

(57) 【Abstract】 PROBLEM TO BE SOLVED: Combined effects such as a lipid peroxide production inhibitory action, an SOD-like activity action, a collagen synthesis promoting action, and an ultraviolet damage recovery action, which have been conventionally required, as an index of the skin anti-aging effect. Mochi, normalizing turnover for human skin, reducing unevenness of area between keratinocytes to prevent skin dullness and stains, and improving fine lines by activating skin cells. (EN) Provided is an anti-aging cosmetic which has excellent effects and an effect of improving split ends, stiffness and gloss of hair when used on hair. SOLUTION: An anti-aging cosmetic composition comprising a decomposed product obtained by treating an alkaline extract of processed rice obtained by previously processing rice with one or more enzymes.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an anti-aging cosmetic composition. More specifically, the present invention relates to a cosmetic having an excellent anti-aging effect and suitable for use in basic cosmetics, makeup cosmetics, toiletry products, hair cosmetics, bath products and the like.

[0002]

2. Description of the Related Art Conventionally, the necessity of anti-aging cosmetics has been taken into consideration, but since the mechanism and definition of aging have not been clarified, generally, the measurement of moisturizing condition and elasticity of skin are considered as the moisturization of the skin. It has been determined whether or not to improve the skin condition by using the anti-aging cosmetic by measuring the above or visually observing the skin condition.

In recent years, research on aging has progressed, and skin aging is mainly caused by a collagen cross-linking reaction, a decrease in mucopolysaccharides such as hyaluronic acid, and cell damage due to ultraviolet rays. It has become known that skin aging cannot be sufficiently prevented just by mixing biochemical products such as mucopolysaccharides and collagen and synthetic polymer products and trying to retain water like conventional cosmetics. It became clear.

[0004]

DISCLOSURE OF THE INVENTION The present invention has been made in view of the above-mentioned prior art, and biosynthesizes collagen, which is one of the important components of the dermis, by acting on fibroblasts in the skin. In addition to preventing skin troubles by suppressing lipid peroxides, it also recovers the damage of skin cells unknowingly caused by daily ultraviolet rays and maintains the youthful skin condition. It is an object to provide a cosmetic having a cosmetic effect.

[0005]

[Means for Solving the Problems] The present invention contains a decomposed product obtained by treating an alkaline extract of processed rice obtained by previously processing rice with one or more enzymes. Anti-aging cosmetics.

[0006]

BEST MODE FOR CARRYING OUT THE INVENTION As described above, the anti-aging cosmetic composition of the present invention is obtained by treating an alkaline extract of processed rice obtained by previously processing rice with one or more enzymes. The obtained decomposition product is blended.

The processed rice used in the present invention is obtained by processing rice in advance, and one of the major characteristics of the present invention is that the processed rice is used.

In this specification, the processing of rice means
For example, steam heat treatment, roasting treatment, pressure treatment, degreasing treatment,
It refers to steam drying treatment, protein reduction treatment, starch removal or starch reduction treatment, germination treatment, coating treatment, etc. Processed rice refers to rice that has been subjected to such treatment in advance.

The concept of rice used in the present invention is as follows.
Examples include brown rice, white rice, and polished rice.

In the steam heat treatment, for example, 50 to 50% of rice is used.
After soaking in warm water heated to about 80 ° C. for about 5 to 20 hours, heat treatment is preferably performed with steam at about 80 to 150 ° C., more preferably at about 100 to 120 ° C. for about 20 to 40 minutes, and then, for example, heaven. It is desirable to dry it by using hot air or the like.

[0011] In the roasting treatment, for example, rice is preferably about 100 to 350 ° C, more preferably 120 to 3 ° C.
It is desirable to carry out heating to about 00 ° C. and roasting.

The roasting treatment includes, for example, roasting and crushing treatment and roasting and expanding treatment. The roasting and crushing treatment may be carried out, for example, by crushing the product obtained by the roasting treatment as described above with a mill. The roasting / expanding treatment may be carried out, for example, by performing the roasting treatment under pressure of about 1000 to 1500 mmHg in a pressure-resistant expansion device, and then reducing the pressure to normal pressure.

In the pressure treatment, for example, rice is treated under a high pressure of preferably about 2000 to 4000 mmHg, more preferably about 2500 to 3500 mmHg, preferably about 1 to 12 hours, more preferably about 2 to 6 hours. It is desirable to perform it after washing with water and drying.

In the degreasing treatment, for example, rice is treated with an organic solvent such as methanol, ethanol, propanol, n-butanol, acetone, ethers and ethyl acetate in an amount of 10 to 10.
It is immersed for about 30 hours and then filtered, preferably 20 to 8
It is desirable to perform drying at a low temperature of about 0 ° C, more preferably about 30 to 60 ° C.

The steam-drying treatment is preferably carried out, for example, by steaming rice, that is, cooking rice by a usual method and then drying it.

The drying in the steam-drying treatment includes, for example, sun drying, hot air drying and freeze drying. The sun drying is, for example, 1 to 1 of cooked rice in the sun.
It may be dried for about 3 days. The hot air drying is performed, for example,
The cooked rice may be dried rapidly with hot air of preferably about 50 to 100 ° C, more preferably about 60 to 80 ° C for about 30 to 120 minutes. The freeze-drying is
For example, cooked rice is preferably -50 to -20 ° C.
The temperature is rapidly frozen to about 40 to -30 ° C, preferably 0.005 to 2 mmHg, and more preferably 0.01 to 1 mmHg. It may be raised to room temperature.

The protein-reducing treatment is carried out, for example, by inoculating rice with lactic acid bacteria and culturing in a conventional manner to assimilate the protein. Usually, about 10 to 50% by weight of the protein contained in rice is converted into peptides. It is desirable to convert it.

The starch removal or starch reduction treatment is carried out, for example, by polishing rice using a rice polishing machine or the like and treating it until its weight becomes about 40 to 60% by weight of the original weight, It is desirable to collect the pulverized product (white bran) from which about 30 to 90% by weight of the starch is removed.

The germination treatment is carried out by, for example, holding rice at a temperature of about 10 to 30 ° C. on a substrate containing an appropriate amount of water for 1 to 2
It is desirable to germinate to a size of about mm and to separate the germinated portion.

In the present invention, the processing of rice may be carried out by performing each of the above treatments alone or by appropriately combining two or more treatments.

In addition, a suitable method may be selected from the various treatment methods described above according to the type of rice to be processed.

Next, the processed rice thus obtained is subjected to an alkali extraction treatment.

The solvent used in the alkali extraction treatment is, for example, water such as purified water; 1 such as ethanol.
Monohydric lower alcohols; monohydric higher alcohols such as oleyl alcohol, stearyl alcohol, octyldodecanol; polyols such as ethylene glycol, propylene glycol, glycerin, 1,3-butylene glycol; ketones such as acetone; acetic acid Examples thereof include esters such as ethyl; hexane; hydrocarbon solvents such as benzene; halogenated hydrocarbon solvents such as chloroform. These can be used alone or in admixture of two or more. Among them, purified water or a mixture of purified water and one or more selected from ethanol, glycerin and 1,3-butylene glycol is used because it can be widely applied to cosmetics. Solvents are preferred.

When the mixed solvent is used, for example, in the case of a mixed solvent of purified water and ethanol, the volume ratio of the two is 1: 1 to 25: 1, and the mixed solvent of purified water and glycerin is the same. In some cases, the capacity ratio of both is 1: 1 to 15 :.
1. In the case of a mixed solvent of purified water and 1,3-butylene glycol, the volume ratio of the two is preferably 1: 1 to 15: 1.

When carrying out the alkali extraction treatment, a pH adjusting agent such as sodium hydroxide, sodium carbonate or potassium hydroxide is used to set the pH of the solution to be subjected to the extraction treatment to about 7.5 to 14. do it. Among these pH adjusters, the target pH at low concentration
Sodium hydroxide and sodium carbonate are preferred because they can be adjusted so that

The time required for the alkali extraction treatment varies depending on the type of solvent and pH adjuster used, the target pH, the extraction temperature, etc., and cannot be determined unconditionally. For example, when the pH is 8.5-14. In this case, it is desirable that the temperature is usually 6 hours to 7 days, preferably 24 to 48 hours at room temperature. The extraction temperature is about 4 to 40 ° C, preferably about 4 to 20 ° C.

Next, the alkali extract obtained as described above is treated with one or more enzymes.

Examples of the enzyme include actinases such as actinase, pepsins such as pepsin, trypsin such as trypsin and chymotrypsin, papains such as papain and chymopapain, glycylglycine peptidase, carboxypeptidase, aminopeptidase. Examples thereof include peptidases and the like, proteolytic enzymes such as bromelain, and among these, one kind or two or more kinds can be selected and used. These enzymes are preferably used from the viewpoint that the anti-aging cosmetic composition containing the obtained degradation product is further excellent in storage stability and safety, and among them, a combination of actinase, pepsin and trypsin is particularly preferable.

When treating with, for example, two or more kinds of enzymes, usually one kind of enzyme is used at a time.

The amount of the enzyme used per time when performing the enzyme treatment varies depending on the kind of the enzyme used, etc.
Considering its action and effect, the alkaline extract 100
About 0.0005 part or more, preferably about 0.001 part or more, and about 0.05 part or less, preferably about 0.005 part or less with respect to parts (parts by weight, the same hereinafter), and 0 in total. It is preferably about 0.003 to 0.015 parts.

The time required for the enzyme treatment varies depending on the type of the enzyme used and the decomposition temperature and cannot be determined unconditionally, but it is usually about 1 to 24 hours, preferably about 2 to 4 hours per enzyme. Is desirable. The decomposition temperature of the above-mentioned enzymes is about 30 to 50.
° C.

Further, in the present invention, in addition to subjecting the obtained alkali extract to the enzyme treatment, the enzyme is treated during the alkali extraction treatment of the processed rice, that is, in the solvent for the alkali extraction treatment containing the processed rice. While adding and extracting
A method of performing enzyme treatment can also be adopted.

The solution containing the obtained decomposition product is preferably adjusted to a pH of about 4 to 8 from the viewpoint of safety to the skin.

The decomposed product thus obtained may be blended as it is with an anti-aging cosmetic composition, for example, concentrated under reduced pressure to adjust the concentration, and then blended, for example, a freeze-drying method or a spray-drying method. You may mix | blend what was pulverized by.

The amount of the above-mentioned decomposed product blended into the anti-aging cosmetic composition varies depending on the kind of the intended cosmetic product and cannot be determined unconditionally, but the anti-aging effect due to the incorporation of the decomposed product is In order to sufficiently express the amount, 0.000 in terms of solid content is added to 100 parts of the anti-aging cosmetic.
It is desirable that the amount is 1 part or more, preferably 0.001 part, and in order to be stably mixed in the cosmetic, it is 5 parts or less, preferably 1 part or less, in terms of solid content, relative to 100 parts of the antiaging cosmetic. Is desirable.

The anti-aging cosmetic composition of the present invention contains the above-mentioned decomposed product. In the present invention, in addition to the decomposed product, for example, an excipient generally used in cosmetics, In addition to fragrances, oils and fats, surfactants, moisturizers, pH adjusters, thickeners, preservatives, antioxidants, UV absorbers, pigments, excipients, emulsifiers, animal and plant derived ingredients, vitamins, amino acids Various kinds of cosmetic ingredients such as the above can be appropriately blended with the antiaging cosmetic.

Examples of the oils and fats include paraffins generally used in cosmetics such as liquid paraffin,
Vegetable oils such as cetanol, avocado oil, olive oil, jojoba oil, and coconut oil; beef tallow, lard, horse fat, turtle oil,
Animal fats and oils such as mink oil, parserine oil and squalane; synthetic fats and oils such as methyl polysiloxane, behenyl alcohol, glyceryl tricaprylic caprate, glyceryl trioctanoate, glyceryl triisopalmitate, and silicone oil.

Examples of the above-mentioned surfactants include anionic surfactants such as sodium lauryl sulfate, triethanolamine lauryl sulfate and diethanolamide lauric acid; stearyl trimethyl ammonium chloride, cetyl trimethyl ammonium chloride, benzalkonium chloride and the like. Cationic surfactants; nonionic surfactants such as glyceryl monostearate, sorbitan monostearate, polyoxyethylene (20) sorbitan monostearate, polyoxyethylene hydrogenated castor oil, sucrose ester, fatty acid amide, etc. Can be given.

Examples of the moisturizer include glycerin, propylene glycol, 1,3-butylene glycol,
Synthetic moisturizers such as sodium pyrrolidone carboxylic acid and pantothetein-S-sulfonate; natural moisturizers such as hyaluronic acid, collagen, elastin, placenta extract, royal jelly and microbial fermentation liquid.

Examples of the pH adjusting agent include organic acids such as sodium citrate and citric acid, and salts thereof.

Examples of the thickener include carboxymethyl cellulose, hydroxymethyl cellulose, hydroxyethyl cellulose, carboxyvinyl polymer,
Examples thereof include polyvinyl alcohol, tragacanth gum, sodium alginate, carrageenan and the like.

Examples of the preservatives include paraoxybenzoic acid esters such as methylparaben, ethylparaben, propylparaben and butylparaben, phenoxyethanol, ethanol and dehydroacetic acid.

Examples of the antioxidant include vitamin E, butylhydroxytoluene (BHT), butylhydroxyanisole (BHA) and the like.

Examples of the pigment include red iron oxide, yellow iron oxide, black iron oxide, titanium oxide, nylon powder, sericite, mica, talc and the like.

Examples of the excipient include mannitol, sodium sulfate and the like.

Examples of the emulsifier include soybean lecithin and the like.

The blending amount of each of these cosmetic ingredients varies depending on the intended use of the anti-aging cosmetic, and cannot be unconditionally determined. It is preferably adjusted appropriately according to the intended use.

The anti-aging cosmetic composition of the present invention may have any form and is not particularly limited. However, the anti-aging cosmetic composition of the present invention prevents skin aging and provides a youthful and healthy skin condition. Since it has excellent properties such as maintaining, it is a basic cosmetic such as cream, milky lotion, lotion, essence, face wash, pack; make-up cosmetic such as lipstick, foundation, liquid foundation, press powder; body soap, soap. It can be used as a toiletry product such as.

The anti-aging cosmetic composition of the present invention acts on the hair roots and around the hair, and is also effective for preventing split ends and broken hair and protecting the hair. Therefore, for example, shampoo, conditioner, treatment, conditioner It can be used as a cosmetic for hair such as a cosmetic.

Furthermore, when the above-mentioned decomposed product or dry powder is poured into hot water, the effect of improving the variation in the area between keratinocytes is maintained, and the feeling of heat retention after bathing and the skin condition are more beautiful and smooth. The anti-aging cosmetic composition of the present invention can also be used as a bath product such as a bath agent because it has the effect of normalizing the composition. Thus, when the anti-aging cosmetic composition of the present invention is used as a bath agent, the amount of the decomposed product incorporated into the anti-aging cosmetic product is 100 parts of the anti-aging cosmetic product in consideration of the effect of the decomposed product. On the other hand, it is desirable that it is 0.0001 part or more, preferably 0.001 part or more, and 5 parts or less, preferably 1 part or less, in terms of solid content of the decomposed product. When the bath agent is used, the amount of the bath agent used is preferably adjusted so that the bath agent is usually about 20 to 30 g per 200 liters of hot water.

The decomposition product used in the present invention has an excellent lipid peroxide production inhibitory action on human skin, a superoxide dismutase (hereinafter referred to as SOD) -like activity,
It has a collagen synthesis promoting action and a UV damage recovery action at the same time. Therefore, the anti-aging cosmetic composition of the present invention containing the decomposed product has effects of preventing skin aging and maintaining a youthful and healthy skin condition.
Further, since the decomposed product is prepared from processed rice obtained by processing rice in advance, it is possible to prevent precipitation of insoluble matter over time, and its quality is highly stable. Therefore, the anti-aging cosmetic composition of the present invention containing the decomposed product has an effect of extremely excellent storage stability.

[0052]

EXAMPLES Next, the anti-aging cosmetic composition of the present invention will be explained in more detail based on examples, but the present invention is not limited to these examples.

Preparation Example 1 (Production of Enzymatic Decomposition Product of Alkali Extract of Steam Heat Treated Rice) 500 g of warm water obtained by heating 1000 g of polished rice to 60 to 70 ° C.
After soaking in 0 ml for 10 to 15 hours, the water was drained, and heat treatment was performed with steam at 100 ° C. for 30 minutes.

After this, sun drying was carried out to obtain about 900 g of steam-heat-treated rice.

Next, 100 g of the steam-heat-treated rice was added to 1000 ml of purified water, the pH was adjusted to 11 to 13 with a 0.1N sodium hydroxide aqueous solution, and the mixture was immersed at room temperature for about 24 hours for extraction to extract the extract. (Solid content: about 5% by weight) About 500 ml was obtained.

Next, 5 mg of actinase (optimum pH 8.0) was added to the above extract, and the amount was 1-2 at 35-45 ° C.
Treatment is carried out over time, and then pepsin (optimum pH
2.0) 5 mg was added and treatment was performed at 35 to 45 ° C. for 1 to 2 hours, and finally trypsin (optimum pH 8.
0) 5 mg was added and the treatment was carried out at 35 to 45 ° C. for 1 to 2 hours.

When each enzyme was added, the extract was adjusted to have the optimum pH for each enzyme.

This was filtered to obtain about 300 ml of a pale yellow transparent enzymatic decomposition product solution (solid content: about 3% by weight).

Preparation Example 2 (Production of Enzymatic Decomposition Product of Alkaline Extract of Steamed Hot Air Drying Rice) 1000 g of polished rice was cooked by a usual method to gelatinize the starch in the polished rice and then hot air at 80 ° C. Rapid drying was performed for 60 minutes to obtain about 950 g of steamed hot air dried rice.

Next, 100 g of the steamed hot air dried rice
Was added to 1000 ml of purified water, the pH was adjusted to 11 to 13 with a 0.1N sodium hydroxide aqueous solution, and about 2 at room temperature.
It was immersed for 4 hours for extraction to obtain about 500 ml of an extract (solid content: about 5% by weight).

Next, 5 mg of actinase (optimum pH 8.0) was added to the above extract, and the amount was 1-2 at 35-45 ° C.
Treatment is carried out over time, and then pepsin (optimum pH
2.0) 5 mg was added and the treatment was carried out at 35 to 45 ° C. for 1 to 2 hours, and finally chymotrypsin (optimum pH
8.0) 5 mg was added and the treatment was carried out at 35 to 45 ° C. for 1 to 2 hours.

When each enzyme was added, the extract was adjusted to have the optimum pH for each enzyme.

This was filtered to obtain about 300 ml of a pale yellow transparent enzyme decomposition product solution (solid content: about 3% by weight).

Preparation Example 3 (Production of Enzymatic Decomposition Product of Alkaline Extract of Degreased Rice) After immersing 1,000 g of polished rice in 5000 ml of ethanol for 24 hours, filtering and drying at 50 ° C., about 900 g of defatted rice was obtained. I got it.

Next, 100 g of the defatted rice was added to 1000 ml of purified water, the pH was adjusted to 11 to 13 with a 0.1N sodium hydroxide aqueous solution, and the mixture was immersed at room temperature for about 24 hours for extraction to extract the extract. (Solid content: about 3% by weight) About 3
I got 00 ml.

Next, 5 mg of actinase (optimum pH 8.0) was added to the extract, and the amount was 1-2 at 35-45 ° C.
Treatment is carried out over time, and then pepsin (optimum pH
2.0) 5 mg was added and treatment was performed at 35 to 45 ° C. for 1 to 2 hours, and finally trypsin (optimum pH 8.
0) 5 mg was added and the treatment was carried out at 35 to 45 ° C. for 1 to 2 hours.

When each enzyme was added, the extract was adjusted to have the optimum pH for each enzyme.

This was filtered to obtain about 300 ml of a pale yellow transparent enzyme decomposition product solution (solid content: about 3% by weight).

Preparation Example 4 (Production of Enzymatic Degradation Product of Alkaline Extract of Steam-Freeze-Dried Rice) 1000 g of polished rice was cooked by a usual method and then -40
It was rapidly frozen to -30 ° C, placed in a vacuum dryer under a reduced pressure of 0.01 to 1 mmHg, and the temperature was raised to room temperature to obtain about 950 g of steam-freeze-dried rice.

Next, 100 g of the steam-boiled freeze-dried rice
Was added to 1000 ml of purified water, the pH was adjusted to 11 to 13 with a 0.1N sodium hydroxide aqueous solution, and about 2 at room temperature.
It was immersed for 4 hours for extraction to obtain about 500 ml of an extract (solid content: about 5% by weight).

Next, about 5 mg of actinase (optimum pH 8.0) was added to the above extract, and the extract was added at 1 to 35 ° C.
The treatment is performed for 2 hours and then pepsin (optimum pH
2.0) 5 mg was added and treatment was performed at 35 to 45 ° C. for 1 to 2 hours, and finally papain (optimum pH 8.0).
5 mg was added and the treatment was carried out at 35 to 45 ° C. for 1 to 2 hours.

When each enzyme was added, the extract was adjusted to have the optimum pH for each enzyme.

This was filtered to obtain about 300 ml of a pale yellow transparent enzyme decomposition product solution (solid content: about 3% by weight).

Preparation Example 5 (Production of Enzymatic Decomposition Product of Alkaline Extract of Low Starch Treated Rice) 1000 g of polished rice was put into a rice polishing machine and ground until the weight became about 50% by weight of the original polished rice. The powder (white rice bran) on the outer side of the milled rice produced at that time was recovered to obtain about 500 g of low-starch-treated rice powder from which about 60% by weight of starch was removed.

Next, the low starch-treated rice powder 10
0 g was added to 1000 ml of purified water, the pH was adjusted to 11 to 13 with a 0.1 N sodium hydroxide aqueous solution, and the mixture was soaked at room temperature for about 24 hours for extraction, and the extraction liquid (solid content: about 5
Wt%) about 500 ml was obtained.

Next, 5 mg of actinase (optimum pH 8.0) was added to the above extract, and the amount was 1-2 at 35-45 ° C.
Treatment is carried out over time, and then pepsin (optimum pH
2.0) 5 mg was added and treatment was performed at 35 to 45 ° C. for 1 to 2 hours, and finally trypsin (optimum pH 8.
0) 5 mg was added and the treatment was carried out at 35 to 45 ° C. for 1 to 2 hours.

When each enzyme was added, the extract was adjusted to have the optimum pH for each enzyme.

This was filtered to obtain about 300 ml of a pale yellow transparent enzyme-decomposed product solution (solid content: about 3% by weight).

Preparation Example 6 (Production of Enzymatic Decomposition Product of Alkaline Extract of Germinated Rice) 100 g of brown rice was placed on a shelf floor covered with a nonwoven fabric containing water and germinated at 20 ° C. for about 1 to 2 mm.
After this, the germinated portion was collected to obtain about 120 g of germinated rice.

Next, 100 g of the germinated rice was added to 1000 ml of purified water, the pH was adjusted to 11 to 13 with a 0.1N sodium hydroxide aqueous solution, and the mixture was soaked at room temperature for about 24 hours for extraction to extract the extract. (Solid content: about 5% by weight) About 5
I got 00 ml.

Next, 5 mg of actinase (optimum pH 8.0) was added to the above extract, and the amount was 1-2 at 35-45 ° C.
Treatment is carried out over time, and then pepsin (optimum pH
2.0) 5 mg was added and treatment was performed at 35 to 45 ° C. for 1 to 2 hours, and finally trypsin (optimum pH 8.
0) 5 mg was added and the treatment was carried out at 35 to 45 ° C. for 1 to 2 hours.

When each enzyme was added, the extract was adjusted to have the optimum pH for each enzyme.

This was filtered to obtain about 300 ml of a pale yellow transparent enzyme decomposition product solution (solid content: about 3% by weight).

Preparation Example 7 (Production of Enzymatic Decomposition Product of Alkaline Extract of Roasted Rice) 1000 g of polished rice was heated at 120 to 300 ° C. for roasting to obtain about 800 g of roasted rice.

Next, 100 g of the roasted rice was added to 1000 ml of purified water, the pH was adjusted to 11 to 13 with a 0.1N sodium hydroxide aqueous solution, and the mixture was immersed at room temperature for about 24 hours for extraction and extraction. Liquid (solid content: about 5% by weight) About 5
I got 00 ml.

Next, 5 mg of actinase (optimum pH 8.0) was added to the above extract, and the amount was 1-2 at 35-45 ° C.
Treatment is carried out over time, and then pepsin (optimum pH
2.0) 5 mg was added and treatment was performed at 35 to 45 ° C. for 1 to 2 hours, and finally trypsin (optimum pH 8.
0) 5 mg was added and the treatment was carried out at 35 to 45 ° C. for 1 to 2 hours.

When each enzyme was added, the extract was adjusted to have the optimum pH for each enzyme.

This was filtered to obtain about 300 ml of a pale yellow transparent enzyme decomposition product solution (solid content: about 3% by weight).

Preparation Example 8 (Production of Enzymatic Decomposition Product of Alkaline Extract of Protein-Lowered Rice) 1000 g of polished rice was inoculated with lactic acid bacteria and cultured by a conventional method to assimilate the protein, which was contained in the polished rice. About 30% by weight of the protein to be converted was converted into a peptide to obtain about 800 g of low-protein-treated rice.

Next, 100 g of the protein-reduced rice
Was added to 1000 ml of purified water, the pH was adjusted to 11 to 13 with a 0.1N sodium hydroxide aqueous solution, and about 2 at room temperature.
It was immersed for 4 hours for extraction to obtain about 500 ml of an extract (solid content: about 5% by weight).

Next, 5 mg of actinase (optimum pH 8.0) was added to the extract, and the amount was 1-2 at 35-45 ° C.
Treatment is carried out over time, and then pepsin (optimum pH
2.0) 5 mg was added and treatment was performed at 35 to 45 ° C. for 1 to 2 hours, and finally trypsin (optimum pH 8.
0) 5 mg was added and the treatment was carried out at 35 to 45 ° C. for 1 to 2 hours.

When each enzyme was added, the extract was adjusted to have the optimum pH for each enzyme.

This was filtered to obtain about 300 ml of a pale yellow transparent enzyme-decomposed product solution (solid content: about 3% by weight).

Preparation Example 9 (Production of Enzymatic Decomposition Product of Alkaline Extract of Pressurized Rice) 1000 g of polished rice was treated under high pressure of 2660 mmHg for 60 minutes to decompose allergen substances, washed with water, dried and added. About 800g of pressure-treated rice was obtained.

Next, 100 g of the pressure-treated rice was added to 1000 ml of purified water, the pH was adjusted to 11 to 13 with a 0.1N aqueous sodium hydroxide solution, and the mixture was immersed at room temperature for about 24 hours for extraction and extraction. Liquid (solid content: about 5% by weight) About 5
I got 00 ml.

Next, 5 mg of actinase (optimum pH 8.0) was added to the above extract, and the amount was 1-2 at 35-45 ° C.
Treatment is carried out over time, and then pepsin (optimum pH
2.0) 5 mg was added and treatment was performed at 35 to 45 ° C. for 1 to 2 hours, and finally trypsin (optimum pH 8.
0) 5 mg was added and the treatment was carried out at 35 to 45 ° C. for 1 to 2 hours.

When each enzyme was added, the extract was adjusted to have the optimum pH for each enzyme.

This was filtered to obtain about 300 ml of a pale yellow transparent enzyme decomposition product solution (solid content: about 3% by weight).

Preparation Example 10 (Production of freeze-dried product of enzymatic decomposition product of alkaline extract of low-starch-treated rice) 100 ml of the enzyme decomposition product solution obtained in Preparation Example 5 was concentrated and then freeze-dried in vacuum. About 2 g of dry powder of the decomposition product was obtained.

Test Examples 1 to 5 The following tests were carried out using the enzyme hydrolyzate solutions obtained in Preparation Examples 1 to 9 as samples.

Test Example 1 (Inhibition of Lipid Peroxide Generation) 1.0 ml of 0.5 M linoleic acid ethanol, 10 ml of 0.2 M phosphate buffer (pH 7.0) and 9.0 ml of ethanol were accurately weighed and analyzed. Stir well in an Erlenmeyer flask with stopper. To this, 5.0 ml of the solution of the enzymatic degradation product obtained in Preparative Examples 1 to 9 which were accurately weighed, was added and stirred sufficiently.

Next, 0.1 ml each of the solution immediately after preparation and the solution left in a constant temperature bath at 40 ° C. for 7 days were accurately weighed, and 75 ml of ethanol 4.7 ml, 30% 0.1 ml of ammonium thiocyanate solution and 0.1 ml of 3.5% hydrochloric acid solution of 0.02 M ferrous chloride were added and thoroughly mixed and stirred, and the absorbance at 500 nm was measured exactly after 3 minutes. The peroxide value index was calculated based on the formula.

[0103]

[Equation 1]

In the formula, T ₇ is the absorbance of the solution to which the enzymatic decomposition product solution was added 7 days after the start of the test, and B ₇
Is the absorbance of the solution to which purified water was added instead of the enzymatic degradation product solution after 7 days from the start of the test, T ₀ is the absorbance of the solution to which the enzymatic degradation product solution was added immediately after the start of the test, and B ₀ is the start of the test The absorbance of the solution to which purified water was added instead of the enzyme decomposition product solution immediately after is shown.

A blank was prepared in the same manner by using purified water instead of the enzymatic decomposition product solution. Table 1 shows the results.

[0106]

[Table 1]

As is clear from the results shown in Table 1,
It can be seen that the enzyme hydrolyzate solutions obtained in Preparation Examples 1 to 9 have an excellent lipid peroxide production inhibitory action.

Test Example 2 (SOD-like activity) 0.15 ml of 1 M Tris-hydrochloric acid buffer solution, 0.30 ml of 1 mM ethylenediaminetetraacetic acid (hereinafter referred to as EDTA) tetrasodium salt solution, 0.2 mM cytochrome C solution
20 ml and 0.30 ml of 1 mM xanthine solution were accurately weighed and thoroughly stirred in an Erlenmeyer flask with a stopper. Preparative examples 1 to 9 accurately weighed in each
0.10 ml of the enzymatic degradation product solution obtained, xanthine oxidase solution (0.01 U / 0.01 ml) 0.05
ml and purified water 1.90 ml, then 550n
The absorbance was measured every 60 seconds in m. The results are shown in graphs A1 to A9 of FIG. 1 (in order, corresponding to the samples containing the enzyme hydrolyzate solutions obtained in Preparation Examples 1 to 9).

A blank was prepared in the same manner by using purified water instead of the enzyme-decomposed product solution. The result is shown in graph B of FIG.

As is clear from the results shown in FIG.
The enzyme-decomposed product solutions obtained in Preparation Examples 1 to 9 have excellent S
It can be seen that it exerts an OD-like activity.

Test Example 3 (Collagen synthesis promoting action) As a basic medium, 5% by volume fetal calf serum (hereinafter, referred to as FBS) -containing minimal Eagle's minimum essential medium (hereinafter, referred to as MEM).
And human dermis-derived fibroblasts were used as cells.

After culturing the cells in basal medium for a certain period of time,
A medium containing 5% by volume of each of the enzyme decomposition product solutions obtained in Preparation Examples 1 to 9 was added, and the mixture was cultured at 37 ° C for 5 days.
During this time, the medium was exchanged once. Moreover, what was cultured using only the basic medium was used as a blank.

With respect to the fibroblasts cultured by the above operation, the amount of collagen and the amount of non-collagen per 10 ^{4 of the} cells were measured, and the action of promoting collagen synthesis was examined. The collagen was quantified using a collagen stain kit manufactured by Cosmo Bio. The results are shown in graphs A1 to A9 of FIG. 2 (corresponding to the samples containing the enzyme hydrolyzate solutions obtained in Preparation Examples 1 to 9 in order) and graph B (blank).

As is clear from the results shown in FIG.
It can be seen that the enzymatic degradation product solutions obtained in Preparation Examples 1 to 9 exhibit an excellent collagen synthesis promoting action.

Test Example 4 (UV damage recovery action) Eagle MEM containing 10% by volume FBS as a basic medium
, And human-derived fibroblasts were used as cells.

After culturing the cells in basal medium for a certain period of time,
A medium containing 10% by volume of each of the enzyme decomposition product solutions obtained in Preparation Examples 1 to 9 was added while irradiating with an ultraviolet ray from an ultraviolet ray lamp (Toshiba health line lamp, manufactured by Toshiba Corporation, output 30w). It was cultured at 0 ° C for 10 days. During that time, the medium was exchanged twice. Moreover, what was cultured using only the basic medium was used as a blank.

Regarding the fibroblasts cultivated by the above-mentioned procedure, trypsin EDTA was used after culturing, and the cells were detached and the number of cells in each sample and the sample containing the enzyme hydrolyzate solution obtained in Preparation Example 1 were removed. The number of resuscitated cells was measured to examine the effect of recovering damage from ultraviolet rays. The results are shown in graphs A1 to A9 of FIG. 3 (corresponding to the samples containing the enzyme hydrolyzate solutions obtained in Preparation Examples 1 to 9 in order), graph B (blank) and graph C (growth cells). .

As is clear from the results shown in FIG.
It can be seen that the enzyme-decomposed product solutions obtained in Preparation Examples 1 to 9 are excellent in the cell damage recovery action by ultraviolet rays.

Test Example 5 (Storage Stability) 50 solutions of the enzyme hydrolyzate solutions obtained in Preparation Examples 1 to 9 were prepared.
It was filled in a screw tube of ml volume and stored at 40 ° C. for 90 days under constant temperature conditions.

The presence or absence of coloration and precipitation in the enzyme-decomposed product solution was visually observed 10 days, 20 days, 30 days, 60 days and 90 days after the start of the test. Further, in Preparation Example 1, the enzyme-decomposed product solution obtained by using normal rice instead of steam heat-treated rice was used as a blank, and stored in the same manner as the enzyme-decomposed product solutions obtained in Preparation Examples 1 to 9, The presence or absence of coloring and precipitation was visually observed. Table 2 shows the results.

[0121]

[Table 2]

As is clear from the results shown in Table 2,
The enzyme-decomposed product solutions obtained in Preparation Examples 1 to 9 showed no color change or precipitation over a long period of time, such as 90 hours, so that the quality did not change and the storage stability was excellent.

Formulation Examples 1 to 14 and Comparative Formulation Examples 1 to 14 Formulation Example 1 (Cream) [(A) Ingredient] (Part) Liquid paraffin 5.0 Hexalan (glyceryl trioctanoate, manufactured by Technoble Co., Ltd.) 4. 0 paraffin 5.0 cetanol 2.0 glyceryl monostearate 2.0 polyoxyethylene (20) sorbitan monostearate 6.0 butylparaben 0.1 natural vitamin E 0.1 [(B) component] Preparation Example 1 Obtained solution of enzymatic decomposition product 20.0 Glycerin 5.0 Sodium carboxymethylcellulose 0.1 Methylparaben 0.1 Moiston C (NMF component, manufactured by Technoble Co., Ltd.) 1.0 Purified water Total 100.0 parts [Component (C)] Fragrance 0.3 After heating each of the components (A) and (B) to 80 ° C. or higher, ( The components (A) and (B) were mixed and stirred. After cooling this to 50 ° C., the component (C) was added and further mixed with stirring to prepare a uniform cream.

Formulation Example 2 (Emulsion) [(A) component] (Part) Liquid paraffin 6.00 Hexalan (glyceryl trioctanoate, manufactured by Technoble Co., Ltd.) 4.00 Jojoba oil 1.00 Polyoxyethylene (20) Sorbitan monostearate 2.00 Soybean lecithin 1.50 Methylparaben 0.15 Ethylparaben 0.03 dl-α-tocopherol acetate 0.10 [(B) component] Enzymatic degradation product solution 30.00 obtained in Preparation Example 2 Glycerin 3.00 1,3-butylene glycol 2.00 Sodium carboxymethyl cellulose 0.30 Sodium hyaluronate 0.01 Amount that makes the total amount of purified water 100.00 parts [(C) component] Fragrance 0.05 Said (A ) And (B) components are heated to 80 ° C, respectively, and then (A) components and ( ) And the component mixture is stirred. After cooling this to 50 ° C., the component (C) was added and further stirred to prepare a uniform emulsion.

Formulation Example 3 (Lotion) [Component] (Part) L-Ascorbyl magnesium phosphate 2.0 Ethanol 10.0 Glycerin 3.0 1,3-Butylene glycol 2.0 Methylparaben 0.2 Citric acid 0. 1 sodium citrate 0.3 carboxyvinyl polymer 0.1 water-soluble placenta extract 1.0 enzyme-decomposed product solution obtained in Preparation Example 10.0 perfume trace amount purified water 100.0 parts by volume Were mixed to prepare a uniform lotion.

Formulation Example 4 (Essence) [Component] (Part) Ethanol 10.0 Glycerin 5.0 1,3-Butylene glycol 1.0 Oleyl alcohol 0.1 Polyoxyethylene sorbitan monolaurate ester (20EO) 1. 0 Methylparaben 0.2 Citric acid 0.1 Sodium citrate 0.3 Carboxyvinyl polymer 0.3 Enzymatic degradation product solution obtained in Preparation Example 1.0 Fragrance Trace water-soluble collagen 1.0 Sodium alginate 0.1 Amount of purified water 100.0 parts in total The above components were mixed to prepare a uniform essence.

Formulation Example 5 (pack) [Component] (Parts) Polyvinyl alcohol 15.0 Hydroxymethylcellulose 5.0 Propylene glycol 5.0 Ethanol 10.0 Methylparaben 0.1 Enzymatic degradation product solution obtained in Preparation Example 5 10.0 Carrageenan 0.5 Fragrance Trace amount Purified water Amount that makes the total amount 100.0 parts The above components were mixed to prepare a uniform pack.

Formulation Example 6 (Facial cleanser) [Components] (Parts) Stearic acid 15.0 Lauric acid 5.0 Myristic acid 15.0 Glyceryl monostearate 4.0 Potassium hydroxide 7.0 Glycerin 8.0 Preparation Enzymatic degradation product solution obtained in Example 6 10.0 Methylparaben 0.2 Sodium alginate 0.1 Purified water Amount that makes the total amount 100.0 parts The above ingredients were heated to 85 ° C. and mixed to obtain a uniform face wash. Prepared.

Formulation Example 7 (Shampoo) [Components] (Parts) Polyoxyethylene lauryl ether Sodium sulfate 25.0 Lauryl sulfate triethanolamine 15.0 Coconut oil fatty acid diethanolamide 4.0 Ethylene glycol monostearate 1.50 Edetate disodium 0.05 Methylparaben 0.10 Fragrance Minor yellow No. 4 Minor amount Enzymatic hydrolyzate solution obtained in Preparation Example 5 50.0 Purified water Total amount of 100.00 parts After heating, the mixture was mixed and stirred to prepare a uniform shampoo.

Formulation Example 8 (Rinse) [Component] (Part) Stearyltrimethylammonium Hydrochloride 1.5 Cetanol 2.0 2-Octyldodecanol 1.0 Cationized Cellulose 0.5 Polyoxyethylene Cetyl Ether 1.0 Propylene Glycol 5.0 Methylparaben 0.1 Fragrance Trace amount Enzymatic degradation product solution obtained in Preparation Example 5 50.0 Purified water Total amount becomes 100.0 parts After heating the above components to 80 ° C. or higher, mixing and stirring To prepare a uniform rinse.

Formulation Example 9 (Treatment) [Components] (Parts) Stearyltrimethylammonium chloride 6.0 Polyvinylpyrrolidone 4.0 Glycerin 1.0 Ethylparaben 0.1 Enzymatic degradation product solution obtained in Preparation Example 50 50. 0 Purified water Amount that makes the total amount 100.0 parts The above components were heated to 80 ° C, and then mixed and stirred to prepare a uniform treatment.

Formulation Example 10 (Hairdressing) [Components] (Parts) Ethanol 60.0 Polyoxypropylene butyl ether (40EO) 20.0 Diisopropanolamine 2.0 Edetate trace amount Fragrance Obtained in Preparation Example 1 Amount of Enzymatic Decomposition Solution to be 100.0 Parts The above components were mixed to prepare a uniform hairdressing composition.

Formulation Example 11 (Bathing agent) [Component] (Part) Sodium sulfate 35.0 Sodium hydrogen carbonate 52.0 Borax 2.0 Sodium carboxymethyl cellulose 1.0 Red 201 Trace amount Perfume Trace amount Preparation Example 10 Amount of dried powder obtained is 100.0 parts by weight The above components were mixed to prepare a uniform bath preparation.

Formulation Example 12 (lipstick) [(A) component] (part) Castor oil 50.0 Octyldodecanol 5.0 Lanolin 5.0 Liquid lanolin 5.0 Beeswax 4.0 Ozokerite 7.0 Candelilla wax 2 0.0 Carnauba wax 1.0 [(B) component] Titanium oxide 1.0 Dye (Red No. 201, etc.) 4.0 Total amount of dry powder obtained in Preparation Example 10 100.0 parts [(C) Ingredient] Fragrance Trace amount After heating the above-mentioned (A) component and (B) component, respectively, (A) component and (B) component were mixed and stirred. This was reheated, the component (C) was added, and the mixture was poured into a mold and rapidly cooled to prepare a lipstick.

Formulation Example 13 (liquid foundation) [(A) component] (parts) Stearic acid 2.4 Propylene glycol monostearate 2.0 Cetostearyl alcohol 0.2 Liquid lanolin 2.0 Liquid paraffin 3.0 Myristic acid Isopropyl 8.5 Propylparaben Appropriate amount [(B) component] Amount that makes the total amount of the enzymatic decomposition product solution obtained in Preparation Example 4 100.0 parts Carboxymethyl cellulose sodium 0.2 Bentonite 0.5 Propylene glycol 4.0 Triethanol Amine 1.1 Methylparaben Appropriate amount [(C) component] Titanium oxide 8.0 Talc 4.0 Coloring pigment Appropriate amount After heating the components (A) and (B), respectively, the components (A) and (B) Were mixed and stirred. This was reheated, the component (C) was added, and the mixture was poured into a mold and stirred until it reached room temperature to prepare a liquid foundation.

Formulation Example 14 (Soap) [Components] (Parts) 80/20 Tallow-coconut soap 66.0 Sodium dilauryl phosphate 2.0 Sodium monolauryl phosphate 17.0 Cocoamidopropyl betaine 10.0 Phosphoric acid L -Ascorbyl Magnesium 0.5 Amount in which the total amount of the enzymatic decomposition product solution obtained in Preparation Example 2 was 100.0 parts The above components were mixed to prepare a uniform soap.

Comparative Prescription Example 1 (Cream) A cream was prepared in the same manner as in Prescription Example 1 except that purified water was used in place of the enzyme-decomposed product solution obtained in Preparation Example 1.

Comparative Prescription Example 2 (Emulsion) An emulsion was prepared in the same manner as in Prescription Example 2 except that purified water was used instead of the enzyme-decomposed product solution obtained in Preparation Example 2.

Comparative Prescription Example 3 (Lotion) A lotion was prepared in the same manner as in Prescription Example 3 except that purified water was used instead of the enzyme-decomposed product solution obtained in Preparation Example 3.

Comparative Formulation Example 4 (Essence) An essence was prepared in the same manner as in Formulation Example 4 except that purified water was used instead of the enzyme-decomposed product solution obtained in Preparation Example 4.

Comparative Prescription Example 5 (Pack) A pack was prepared in the same manner as in Prescription Example 5 except that purified water was used in place of the enzyme-decomposed product solution obtained in Preparation Example 5.

Comparative Prescription Example 6 (Facial Cleanser) A facial cleanser was prepared in the same manner as in Prescription Example 6 except that purified water was used instead of the enzyme-decomposed product solution obtained in Preparation Example 6.

Comparative Prescription Example 7 (Shampoo) A shampoo was prepared in the same manner as in Prescription Example 7 except that purified water was used instead of the enzyme-decomposed product solution obtained in Preparation Example 7.

Comparative Prescription Example 8 (Rinse) A rinse was prepared in the same manner as in Prescription Example 8 except that purified water was used instead of the enzyme-decomposed product solution obtained in Preparation Example 8.

Comparative Prescription Example 9 (Treatment) A treatment was prepared in the same manner as in Prescription Example 9 except that purified water was used instead of the enzyme-decomposed product solution obtained in Preparation Example 9.

Comparative Formulation Example 10 (Hairdressing) A hairdressing preparation was prepared in the same manner as in Formulation Example 10 except that purified water was used in place of the enzyme-decomposed product solution obtained in Preparation Example 1.

Comparative formulation example 11 (bath formulation) A bath formulation was prepared in the same manner as in formulation example 11 except that sodium sulfate was used instead of the dry powder obtained in preparation example 10.

Comparative Prescription Example 12 (Lipstick) A lipstick was prepared in the same manner as in Prescription Example 12 except that nylon powder was used instead of the dry powder obtained in Preparation Example 10.

Comparative Prescription Example 13 (Liquid Foundation) A liquid foundation was prepared in the same manner as in Prescription Example 13 except that purified water was used instead of the enzyme-decomposed product solution obtained in Preparation Example 4.

Comparative Prescription Example 14 (Soap) A soap was prepared in the same manner as in Prescription Example 14 except that purified water was used instead of the enzyme-decomposed product solution obtained in Preparation Example 2.

Example 1 The cosmetic tests obtained in Formulation Examples 1 to 6 and Comparative Formulation Examples 1 to 6 were subjected to the following monitor tests. Table 3 shows the results.

(Monitor Test) 100 randomly selected healthy adult women aged 25 to 57 years were used as subjects.
After using each cosmetic on the facial skin for one day every day,
(A) The effect of improving skin dullness and stains, (b) the effect of improving small wrinkles, and (c) the effect of reducing the variation in the area between corneocytes were examined.

(A) Effect of improving skin dullness and blemishes The skin condition was visually observed and evaluated based on the following evaluation criteria.

(Evaluation Criteria) A: Very improved. B: Improved. C: There is no change. D: A little noticeable. E: It became noticeable.

(B) Effect on small wrinkles The condition of the skin was visually observed and evaluated based on the following evaluation criteria.

(Evaluation Criteria) A: It disappeared cleanly. B: It became a little less noticeable. C: There is no change. D: A little more. E: Increased.

(C) Reduction effect on variation in area between keratinocytes Double-sided paper tape (ny stack, on the left cheek of the subject's face)
(Manufactured by Nichiban Co., Ltd.) was applied, and keratinocytes were exfoliated by the tape stripping method, and the area of each cell was measured. The cell area was calculated for 30 cells, the standard deviation was calculated, and the evaluation was performed based on the following evaluation criteria.

(Evaluation Criteria) A: The standard deviation was reduced by 50%. B: The standard deviation was reduced by 25%. C: There is no change. D: The standard deviation was increased by 25%. E: The standard deviation was increased by 50%.

In the monitor test, none of the subjects had skin abnormalities when the cosmetics obtained in Formulation Examples 1 to 6 were used.

The cosmetics obtained in Formulation Examples 1 to 6 are
None of them changed their condition within one month.

[0161]

[Table 3]

As is clear from the results shown in Table 3,
It can be seen that when the cosmetics obtained in Formulation Examples 1 to 6 are used, the variation in the area between keratinocytes is remarkably reduced, and dullness of skin, spots and fine wrinkles are improved.

Example 2 The following half head test was carried out on the hair cosmetics obtained in Formulation Examples 7 to 10 and Comparative Formulation Examples 7 to 10, respectively. Table 4 shows the results.

(Half-head test) Twenty healthy adult women, 25 to 60 years old, who were randomly sampled were used as test subjects, and each hair cosmetic composition was applied to the hair once a day for 30 days. (D) split ends, (e) Koshi and (f)
The luster was evaluated based on the following evaluation criteria.

(D) Split ends (Evaluation criteria) A: No more. B: It has decreased. C: There is no change. D: A little more. E: Increased.

(E) Elasticity (Evaluation Criteria) A: Strengthened. B: A little stronger. C: There is no change. D: A little weakened. E: It became weak.

(F) Luster (Evaluation criteria) A: Improves. B: A little better. C: There is no change. D: It became a little bad. E: It's getting worse.

[0168] In the half head test, none of the subjects had abnormal hair or scalp when using the cosmetic for hair obtained in Formulation Examples 7 to 10.

The hair cosmetics obtained in Formulation Examples 7 to 10 did not change their condition in 30 days.

[0170]

[Table 4]

As is clear from the results shown in Table 4,
It can be seen that when the cosmetics for hair obtained in Formulation Examples 7 to 10 are used, the split ends, stiffness and gloss of the hair are all improved.

Example 3 Each of the bath agents obtained in Formulation Example 11 and Comparative Formulation Example 11 was subjected to the following monitor test.
Table 5 shows the results.

(Monitoring test) Twenty healthy adult men and women aged 27 to 59 years, randomly selected, were used as subjects, and each bath agent was used for 4 weeks. The reduction effect on the variation of the was investigated. In addition, the bath agent is 25 for 200 liters of hot water.
g was added and dissolved.

(G) Reduction effect on variation in area between keratinocytes: Standard deviation was calculated in the same manner as in Example 1 except that 30 cells on the left flank of the subject were used, and based on the following evaluation criteria. Evaluated.

(Evaluation Criteria) A: The standard deviation was reduced by 50%. B: The standard deviation was reduced by 25%. C: There is no change. D: The standard deviation was increased by 25%. E: The standard deviation was increased by 50%.

In this monitor test, none of the subjects had skin abnormality when the bath preparation obtained in Prescription Example 11 was used.

The bath agent obtained in Formulation Example 11 had a composition of 4
The condition did not change over the weeks.

[0178]

[Table 5]

As is clear from the results shown in Table 5,
It can be seen that when the bath agent obtained in Formulation Example 11 is used, the variation in the area between corneocytes is markedly reduced.

Example 4 The following monitor tests were carried out on the cosmetics obtained in Formulation Examples 12 to 13 and Comparative Formulation Examples 12 to 13, respectively. Table 6 shows the results.

(Monitor Test) 100 healthy adult women aged 25 to 57 who were randomly selected were used as subjects.
Between (h) small wrinkles after daily use of each cosmetic on the facial cheek skin for 1 month, between (li) keratinocytes after daily use on the lips or facial cheek skin for 1 month The reduction effect on the variation of the area was examined.

(H) Effect on small wrinkles The state of the skin was visually observed and evaluated based on the following evaluation criteria.

(Evaluation Criteria) A: It disappeared cleanly. B: It became a little less noticeable. C: There is no change. D: A little more. E: Increased.

(I) Effect of reducing area variation between keratinocytes For the cosmetic preparations of Prescription Example 12 and Comparative Prescription Example 12, 30 cells of the lips of the test subjects and the cosmetic preparations of Prescription Example 13 and Comparative Prescription Example 13 were used. For, the standard deviation was calculated in the same manner as in Example 1 except that 30 cells on the left cheek of the subject's face were used, and evaluation was performed based on the following evaluation criteria.

(Evaluation Criteria) A: The standard deviation was reduced by 50%. B: The standard deviation was reduced by 25%. C: There is no change. D: The standard deviation was increased by 25%. E: The standard deviation was increased by 50%.

In this monitor test, none of the subjects had skin abnormalities when the cosmetics obtained in Prescription Examples 12 to 13 were used.

The cosmetics obtained in Formulation Examples 12 to 13 did not change their condition within one month.

[0188]

[Table 6]

As is clear from the results shown in Table 6,
It can be seen that when the cosmetics obtained in Formulation Examples 12 to 13 are used, the variation in the area between corneocytes is significantly reduced, and fine wrinkles are improved.

[0190]

EFFECTS OF THE INVENTION A decomposed product obtained by treating an alkaline extract of processed rice with one or more enzymes used in the present invention serves as an index of the conventionally required skin anti-aging effect. It also has the effects of inhibiting lipid peroxide production, SOD-like activity, promoting collagen synthesis, and recovering from UV damage. Therefore, the anti-aging cosmetic of the present invention in which the decomposed product is blended does not have a mere moisturizing effect, normalizes the turnover on human skin, and reduces the variation in the area between corneocytes. It has excellent effects such as preventing dullness and stains on the skin and improving fine lines by activating the cells of the skin. In addition, when used as a cosmetic for hair, it has the effect of improving split ends, stiffness, gloss and the like of the hair.

Furthermore, since the decomposed product used in the present invention is obtained from processed rice obtained by processing rice in advance, its quality is highly stable. Therefore, the anti-aging cosmetic composition of the present invention containing the decomposed product has an effect of excellent storage stability.

[Brief description of the drawings]

FIG. 1 is a graph of the samples containing the enzyme hydrolyzate solutions obtained in Preparation Examples 1 to 9 and blanks at 550 nm.
It is a graph which shows the light absorbency for every progress for 0 second.

FIG. 2 is a graph showing the amount of collagen and the amount of non-collagen per 10 ⁴ cells in the samples and blanks containing the enzymatic degradation product solutions obtained in Preparation Examples 1 to 9.

FIG. 3 shows the number of cells in a sample and a blank containing the enzyme decomposition product solution obtained in Preparation Examples 1 to 9 and the number of resuscitated cells in a sample containing the enzyme decomposition product solution obtained in Preparation Example 1. It is a graph shown.

─────────────────────────────────────────────────── ─── Continuation of the front page (51) Int.Cl. ⁶ Identification code Internal reference number FI Technical display area A61K 7/075 A61K 7/075 7/08 7/08 7/48 7/48 7/50 7 / 50 C11D 9/38 C11D 9/38

Claims (2)

[Claims] 1. An anti-aging cosmetic composition comprising a decomposed product obtained by treating an alkaline extract of processed rice obtained by previously processing rice with one or more enzymes. 2. The anti-aging cosmetic composition according to claim 1, wherein the enzyme for treating the alkaline extract is at least one proteolytic enzyme selected from actinases, pepsins, trypsins, papains, peptidases and bromelain. Fee.