JPH09206361A - How to store adsorbent particles - Google Patents
How to store adsorbent particlesInfo
- Publication number
- JPH09206361A JPH09206361A JP8290149A JP29014996A JPH09206361A JP H09206361 A JPH09206361 A JP H09206361A JP 8290149 A JP8290149 A JP 8290149A JP 29014996 A JP29014996 A JP 29014996A JP H09206361 A JPH09206361 A JP H09206361A
- Authority
- JP
- Japan
- Prior art keywords
- adsorbent particles
- adsorbent
- radiation
- particles
- storing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000002245 particle Substances 0.000 title claims abstract description 73
- 239000003463 adsorbent Substances 0.000 title claims abstract description 56
- 230000005855 radiation Effects 0.000 claims abstract description 25
- 238000000034 method Methods 0.000 claims abstract description 21
- 229920001059 synthetic polymer Polymers 0.000 claims abstract description 12
- 229920001577 copolymer Polymers 0.000 claims description 11
- 239000011148 porous material Substances 0.000 claims description 9
- 238000004806 packaging method and process Methods 0.000 claims description 5
- GUTLYIVDDKVIGB-OUBTZVSYSA-N Cobalt-60 Chemical compound [60Co] GUTLYIVDDKVIGB-OUBTZVSYSA-N 0.000 claims description 4
- 229920005992 thermoplastic resin Polymers 0.000 claims 1
- 241000233866 Fungi Species 0.000 abstract description 10
- 239000005022 packaging material Substances 0.000 abstract description 5
- 238000001179 sorption measurement Methods 0.000 abstract description 5
- 230000007774 longterm Effects 0.000 abstract description 3
- 230000002542 deteriorative effect Effects 0.000 abstract 1
- -1 polyethylene Polymers 0.000 description 8
- 239000000126 substance Substances 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 8
- 230000005251 gamma ray Effects 0.000 description 7
- 244000005700 microbiome Species 0.000 description 7
- 239000004698 Polyethylene Substances 0.000 description 6
- 229920000573 polyethylene Polymers 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 238000011109 contamination Methods 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 101100313763 Arabidopsis thaliana TIM22-2 gene Proteins 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- STVZJERGLQHEKB-UHFFFAOYSA-N ethylene glycol dimethacrylate Substances CC(=C)C(=O)OCCOC(=O)C(C)=C STVZJERGLQHEKB-UHFFFAOYSA-N 0.000 description 2
- 230000002538 fungal effect Effects 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 229920000193 polymethacrylate Polymers 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 229920005989 resin Polymers 0.000 description 2
- 239000011347 resin Substances 0.000 description 2
- CHRJZRDFSQHIFI-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;styrene Chemical compound C=CC1=CC=CC=C1.C=CC1=CC=CC=C1C=C CHRJZRDFSQHIFI-UHFFFAOYSA-N 0.000 description 1
- DBCAQXHNJOFNGC-UHFFFAOYSA-N 4-bromo-1,1,1-trifluorobutane Chemical compound FC(F)(F)CCCBr DBCAQXHNJOFNGC-UHFFFAOYSA-N 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 1
- 101000671819 Homo sapiens Ubiquitin carboxyl-terminal hydrolase 36 Proteins 0.000 description 1
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 102100040109 Ubiquitin carboxyl-terminal hydrolase 36 Human genes 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 229920001429 chelating resin Polymers 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000020169 heat generation Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000001678 irradiating effect Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 229920000139 polyethylene terephthalate Polymers 0.000 description 1
- 239000005020 polyethylene terephthalate Substances 0.000 description 1
- 229920000098 polyolefin Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 239000001965 potato dextrose agar Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 229920003002 synthetic resin Polymers 0.000 description 1
- 239000000057 synthetic resin Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
Abstract
(57)【要約】
【課題】 合成高分子製多孔質粒子よりなる吸着剤粒子
を、吸着性能を低下させることなく、かつ、真菌類を付
着することなく長期保管する方法の提供。
【解決手段】 袋等の包装材中に密封された状態の合成
高分子製多孔質粒子に放射線を５〜２０ｋＧｙの線量照
射に密封状態のまま保管する。(57) Abstract: [PROBLEMS] To provide a method for long-term storage of adsorbent particles composed of synthetic polymer porous particles without deteriorating the adsorption performance and without adhering fungi. SOLUTION: A synthetic polymer porous particle in a sealed state in a packaging material such as a bag is stored in a sealed state by irradiation with a radiation dose of 5 to 20 kGy.
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、合成高分子製多孔
質粒子よりなる吸着剤粒子の保管方法に関する。TECHNICAL FIELD The present invention relates to a method for storing adsorbent particles composed of synthetic polymer porous particles.
【０００２】[0002]
【従来の技術】スチレン−ジビニルベンゼン共重合体、
メチルメタクリレート・エチレングリコールジメタクリ
レート共重合体等の架橋合成高分子の多孔質粒子は、従
来から、その大きな比表面積を利用することにより、液
体中の溶質分子、例えば抗生物質、蛋白質因子の医薬や
気体分子の吸着捕集に有効であり、吸着剤粒子として気
体あるいは液から有害な不要物の除去や有用物の回収、
濃縮分離等の用途に幅広く利用されている（ＵＳＰ３６
４９４５６、ＵＳＰ３７１６４８２、ＵＳＰ３４５８９
７６、ＵＳＰ４１９９４４９、ＵＳＰ３７９４５８
４）。しかし、多孔質粒子は、その吸着性ゆえに多孔質
粒子の搬送中、又は保管中、包装中に真菌類等の微生物
を表面に吸着しやすく、また、高表面積のためこれらの
増殖を助長する傾向がある。多孔質粒子の表面域あるい
は多孔質粒子の内表面において、真菌類、胞子等の微生
物がひとたび増殖を開始すると、多孔質粒子の表面ある
いは細孔が微生物体により汚染遮蔽され、吸着剤として
の機能が著しく低下し、実質的に使用することができな
くなる。また、吸着剤粒子中に発生した微生物は吸着剤
粒子表面上に濃縮した有機物を分解変性したり、あるい
は微生物から産出される代謝物等により吸着された有用
物、例えば抗生物質、蛋白質因子が汚染される等、吸着
剤粒子の使用の面で大きな障害となっていた。PRIOR ART Styrene-divinylbenzene copolymer,
Conventionally, porous particles of cross-linked synthetic polymers such as methyl methacrylate / ethylene glycol dimethacrylate copolymer have been used by taking advantage of their large specific surface areas, solute molecules in liquids such as antibiotics and protein factor drugs. It is effective for adsorption and collection of gas molecules, and it removes harmful unwanted substances from gas or liquid as adsorbent particles and collects useful substances.
Widely used for applications such as concentration and separation (USP36
49456, USP3716482, USP34589.
76, USP4199449, USP379458
4). However, the porous particles tend to adsorb microorganisms such as fungi on the surface during transportation, storage, or packaging of the porous particles due to their adsorptivity, and also tend to promote their growth due to their high surface area. There is. Once microorganisms such as fungi and spores start to grow on the surface area of the porous particles or on the inner surface of the porous particles, the surfaces or pores of the porous particles are contaminated and shielded by the microorganisms, and they function as an adsorbent. Is significantly reduced, making it virtually unusable. In addition, the microorganisms generated in the adsorbent particles decompose or modify the organic substances concentrated on the surface of the adsorbent particles, or the useful substances adsorbed by the metabolites produced by the microorganisms, such as antibiotics and protein factors, are contaminated. This has been a major obstacle to the use of adsorbent particles.
【０００３】吸着剤粒子の真菌類、胞子等による汚染
は、吸着剤粒子に付着した菌体、胞子等の微小生物体が
増殖することにより発生する。吸着剤粒子を長期保管す
る方法として、高濃度の塩化ナトリウム水溶液中に吸着
剤粒子を保管して微生物体の増殖を停止させる方法、あ
るいは水酸化ナトリウムや次亜塩素酸ナトリウム水溶液
で吸着剤粒子を洗浄して微生物体を死滅させる方法等の
薬剤による処理が行われてきた。しかし、吸着剤粒子に
対して、これらの薬剤を使用する方法では吸着剤粒子に
付着した微生物体を完全に除去することはできず、ま
た、防菌処理後にクリーニングして薬剤を除去すると、
搬送や包装時に空気中あるいは水中からの再汚染を防ぐ
ことが困難であった。さらに、薬剤による処理は、しば
しば吸着剤粒子の基材である合成樹脂を腐食分解させた
り多孔性を変化させ、その吸着機能が実質的に失われる
原因となっていた。Contamination of the adsorbent particles with fungi, spores and the like occurs when microscopic organisms such as fungi and spores attached to the adsorbent particles grow. As a method of storing the adsorbent particles for a long period of time, a method of storing the adsorbent particles in a high-concentration sodium chloride aqueous solution to stop the growth of microorganisms, or a method of storing the adsorbent particles with sodium hydroxide or sodium hypochlorite aqueous solution Treatment with chemicals such as a method of washing to kill microbial bodies has been performed. However, with respect to the adsorbent particles, it is not possible to completely remove the microorganisms adhering to the adsorbent particles by the method of using these agents, and if the agent is removed by cleaning after the antibacterial treatment,
It was difficult to prevent recontamination from air or water during transportation and packaging. Further, the treatment with a chemical often causes the synthetic resin, which is the base material of the adsorbent particles, to be decomposed by corrosion or to change the porosity, and the adsorption function thereof is substantially lost.
【０００４】[0004]
【発明が解決しようとする課題】本発明は、合成高分子
製多孔質粒子よりなる吸着剤粒子を確実且つ長期にわた
って汚染することなく保管する方法を提供することにあ
る。即ち、多孔質粒子に残存する真菌類等の微小生物体
を実質上死滅させ、さらに長期にわたって真菌類等によ
る汚染を防止する保管方法を提供することを課題とす
る。本発明者らは、かかる課題を解決する方法として放
射線照射による方法を検討した。吸着剤粒子のような機
能性の多孔質粒子については、吸着剤粒子に直接放射線
を照射すると照射による化学反応あるいは発熱による作
用のために、多孔質粒子の吸着性能が失活したり、強度
が低下したりするおそれがあった。我々は、種々検討の
結果、吸着剤粒子を包装材で密封状態で放射線を照射す
ることにより、吸着剤粒子の多孔質構造を損なうことな
く、真菌類等に対し防菌処理でき、長期に亘って保管す
ることができることを見出し、本発明を完成させた。SUMMARY OF THE INVENTION It is an object of the present invention to provide a method for reliably and long-term storage of adsorbent particles made of synthetic polymer porous particles without contamination. That is, it is an object of the present invention to provide a storage method that substantially kills microscopic organisms such as fungi remaining on the porous particles and prevents contamination with fungi or the like for a long period of time. The present inventors have examined a method using radiation irradiation as a method for solving such a problem. For functional porous particles such as adsorbent particles, when the adsorbent particles are directly irradiated with radiation, the adsorption performance of the porous particles may be deactivated or the strength may decrease due to the chemical reaction or heat generation effect of the irradiation. There was a risk of a drop. As a result of various investigations, we have shown that by irradiating the adsorbent particles in a sealed state with radiation, it is possible to perform fungicidal treatment against fungi and the like without impairing the porous structure of the adsorbent particles, and for a long period of time. The present invention has been completed based on the finding that they can be stored as
【０００５】[0005]
【課題を解決するための手段】本発明は、合成高分子製
多孔質粒子よりなる吸着剤粒子を予め密封包装し、この
密封状態の吸着剤粒子に放射線を照射し、この密封状態
を保ったままで吸着剤粒子を保管する方法を提供するも
のである。According to the present invention, adsorbent particles made of synthetic polymer porous particles are sealed and packaged in advance, and the sealed adsorbent particles are irradiated with radiation to keep the sealed state. The present invention also provides a method for storing adsorbent particles.
【０００６】[0006]
【作用】密封状態で吸着剤粒子は放射線照射され、その
密封状態を保ったままで吸着剤粒子は保管されるので、
真菌類の発生を防止できる。又、吸着剤粒子の保管中、
密封されているので、包装材の外部からの汚染が防止さ
れる。[Function] Since the adsorbent particles are irradiated with radiation in a sealed state and the adsorbent particles are stored while maintaining the sealed state,
The generation of fungi can be prevented. Also, during storage of the adsorbent particles,
Since it is hermetically sealed, external contamination of the packaging material is prevented.
【０００７】[0007]
【発明の実施の形態】以下に本発明を詳細に説明する。
多孔質粒子の基材の合成高分子は吸着剤として使用でき
る架橋された合成高分子粒子である。かかる合成高分子
としては、例えば、スチレン−ジビニルベンゼン共重合
体及びその臭素化反応物、エチレングリコールジメタク
リレート重合体、メチルメタクリレート−エチレングリ
コールジメタクリレート共重合体、ステアリルメタクリ
レート−トリメチロールプロパントリメタクリレート共
重合体等の架橋ポリスチレン粒子または架橋ポリメタク
リレート粒子、アクリロニトリル−ジビニルベンゼン共
重合体、及びフェノール−ホルマリン縮合物からなるも
のが挙げられる。これらの中でもスチレン−ジビニルベ
ンゼン共重合体が好ましい。DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will be described below in detail.
The synthetic polymer of the base material of the porous particles is a crosslinked synthetic polymer particle that can be used as an adsorbent. Examples of such synthetic polymers include styrene-divinylbenzene copolymer and its brominated reaction product, ethylene glycol dimethacrylate polymer, methyl methacrylate-ethylene glycol dimethacrylate copolymer, stearyl methacrylate-trimethylolpropane trimethacrylate copolymer. Examples include crosslinked polystyrene particles or crosslinked polymethacrylate particles such as polymers, acrylonitrile-divinylbenzene copolymers, and phenol-formalin condensates. Of these, a styrene-divinylbenzene copolymer is preferable.
【０００８】本発明における多孔質の吸着剤粒子は、比
表面積が１０〜１，５００ｍ2 ／ｇ、好ましくは３００
〜１，２００ｍ2 ／ｇ、細孔容積が０．１〜３ｍｌ／
ｇ、好ましくは０．５〜２ｍｌ／ｇの多孔性を有し、多
孔質粒子の粒子径が５〜１，０００μｍ、好ましくは５
０〜６００μｍのものが好ましい。これらの合成高分子
製多孔質粒子よりなる吸着剤粒子は、例えば、スチレン
−ジビニルベンゼン共重合体系のものは、三菱化学
（株）よりＤＩＡＩＯＮ ＨＰ２０、ＤＩＡＩＯＮ Ｈ
Ｐ２１、ＤＩＡＩＯＮ ＨＰ２０ＳＳ、Ｒｏｈｍ＆Ｈａ
ａｓ社よりＡｍｂｅｒｌｉｔｅ ＸＡＤ４、Ａｍｂｅｒ
ｌｉｔｅ ＸＡＤ１６の商品名で、又、ポリメタクリレ
ート系のものは、三菱化学（株）よりＤＩＡＩＯＮ Ｈ
Ｐ２ＭＧ、Ｒｏｈｍ＆Ｈａａｓ社よりＡｍｂｅｒｌｉｔ
ｅ ＸＡＤ７の商品名で入手することができる。The porous adsorbent particles in the present invention have a specific surface area of 10 to 1,500 m 2 / g, preferably 300.
~ 1,200 m 2 / g, pore volume 0.1-3 ml /
g, preferably 0.5 to 2 ml / g, and the porous particles have a particle size of 5 to 1,000 μm, preferably 5
It is preferably from 0 to 600 μm. The adsorbent particles composed of these synthetic polymer porous particles are, for example, those of styrene-divinylbenzene copolymer type, manufactured by Mitsubishi Chemical Co., Ltd. DIAION HP20, DIAION H.
P21, DIAION HP20SS, Rohm & Ha
Amberlite XAD4, Amber from as
lite XAD16, a polymethacrylate type, is available from Mitsubishi Chemical Corporation as DIAION H
Amberlit from P2MG, Rohm & Haas
e It is available under the trade name of XAD7.
【０００９】照射する放射線の線種としては、コバルト
６０を線源として使用するγ線照射が実用的であり好ま
しい。放射線の照射線量は、線源の量及び線源からの距
離によって、あるいは照射時間を制御することによっ
て、照射線量率を連続的に適宜調整することができる。
照射線量は、２〜４０ｋＧｙ（放射線吸収量：グレイ）
が好ましく、５〜２０ｋＧｙがより好ましい。この範囲
を外れると、長期安定性の付与が不十分であったり、γ
線量が必要以上に照射されたり、多孔質粒子が変質した
りするので好ましくない。As the radiation type of the irradiation, γ-ray irradiation using cobalt 60 as a radiation source is practical and preferable. The irradiation dose of the radiation can be continuously and appropriately adjusted by adjusting the amount of the radiation source and the distance from the radiation source or by controlling the irradiation time.
Irradiation dose is 2-40 kGy (Radiation absorption: Gray)
Is preferable, and 5-20 kGy is more preferable. If it deviates from this range, the long-term stability may be insufficiently imparted or γ
It is not preferable because the dose is irradiated more than necessary and the porous particles are altered.
【００１０】放射線照射による保存安定効果を長時間に
わたって持続させるためには、照射を行う前に多孔質粒
子を包装材で密封包装する。この密封包装の際には、窒
素ガス、イオン交換水等の不活性物で袋や容器の内部を
置換して吸着剤粒子を密封してもよい。包装としては、
胞子等の微生物体が外部から直接侵入し付着するのを防
止できる機能が必要であり、具体的にはポリエチレン、
ポリプロピレン等のポリオレフィンやポリエチレンテレ
フタレート等の樹脂フィルムまたは袋、容器等の樹脂成
形体、並びにガラス瓶、缶または樽等の包装材が用いら
れる。照射処理には、既知の設備をそのまま使用するこ
とができる。即ち、乾燥した、あるいは水等で湿潤した
多孔質粒子包装材で密封梱包した後、放射線照射設備に
移送し上記の照射条件で処理し、そのままの密封状態を
保って保管する。袋のフィルムの肉厚は、８〜３００μ
ｍ、好ましくは２０〜１２０μｍ、容器の壁厚は、０．
３〜２ｍｍが好ましい。−５０〜＋５０℃、より好まし
くは−１５℃〜常温である。In order to maintain the storage stability effect of irradiation with radiation for a long time, the porous particles are hermetically packed with a packaging material before irradiation. At the time of this hermetic packaging, the adsorbent particles may be hermetically sealed by substituting the interior of the bag or container with an inert material such as nitrogen gas or ion-exchanged water. As for packaging,
It is necessary to have a function to prevent microbial bodies such as spores from invading and adhering directly from the outside. Specifically, polyethylene,
Resin films or bags such as polyolefins such as polypropylene and polyethylene terephthalate, resin moldings such as containers, and packaging materials such as glass bottles, cans or barrels are used. Known equipment can be used as it is for the irradiation treatment. That is, after being sealed and packed with a porous particle packaging material that is dried or moistened with water or the like, it is transferred to a radiation irradiation facility, processed under the irradiation conditions described above, and stored in the same sealed state. The film thickness of the bag is 8-300μ
m, preferably 20 to 120 μm, and the wall thickness of the container is 0.
It is preferably 3 to 2 mm. -50 to + 50 ° C, more preferably -15 ° C to room temperature.
【００１１】[0011]
【実施例】以下に、本発明の方法について代表的な実施
例および比較例を示し、更に具体的に説明する。なお、
これらは単なる例示であり、本発明は、特にこれらの実
施例によって、限定されるものではない。EXAMPLES Hereinafter, the method of the present invention will be described in more detail by showing representative examples and comparative examples. In addition,
These are merely examples, and the present invention is not limited to these examples in particular.
【００１２】実施例１〜７、比較例１ 下記の実験は、初期条件の制御として、菌体を吸着剤粒
子と一緒に包装して行った。Ａｓｐｅｒｇｉｌｌｕｓ
ｎｉｇｅｒ ＭＣＩ １５２８菌を培養したスラント４
本に減菌水１４ｍｌを加え、胞子を掻き採って得た懸濁
液を、遠心チューブに移し、１２，０００回転で１０分
間遠心分離を行い、上澄み液を取り除いた。この操作を
３回繰り返すことにより菌胞子の洗浄を行って菌胞子を
回収し、１０ｍｌの減菌水中に加え、分散させて溶液と
したものを菌液とした。Examples 1 to 7 and Comparative Example 1 The following experiment was carried out by wrapping bacterial cells together with adsorbent particles as a control of initial conditions. Aspergillus
slant 4 culturing Niger MCI 1528
14 ml of sterilized water was added to the book, and the suspension obtained by scraping off the spores was transferred to a centrifuge tube and centrifuged at 12,000 rpm for 10 minutes to remove the supernatant. By repeating this operation three times, the spores were washed to collect the spores, and the spores were added to 10 ml of sterilized water and dispersed to obtain a solution, which was used as a bacterium solution.
【００１３】市販のスチレン−ジビニルベンゼン共重合
体の合成吸着剤“ダイヤイオンＨＰ２０”（三菱化学
（株）製 比表面積６１７ｍ2 ／ｇ、平均細孔容積１．
３４ｍｌ／ｇ、平均粒子径約５００μｍ）を水膨潤状態
で１００ｍｌポリエチレン製袋に計量した。吸着剤１０
０ｍｌに対して前記菌液１ｍｌを添加した後、ポリエチ
レン袋の開放口をヒートシール法により密閉し、菌を吸
着剤中に均一に分散付着させた。この密封したポリエチ
レン袋に、コバルト６０線源を有する照射装置により、
下記表１に示す照射線量率及び照射時間でγ線照射を行
った。Commercially available synthetic adsorbent of styrene-divinylbenzene copolymer "Diaion HP20" (manufactured by Mitsubishi Chemical Co., Ltd., specific surface area 617 m 2 / g, average pore volume 1.
34 ml / g, average particle diameter of about 500 μm) was weighed in a 100 ml polyethylene bag in a water swollen state. Adsorbent 10
After adding 1 ml of the bacterial solution to 0 ml, the opening of the polyethylene bag was sealed by a heat seal method to uniformly disperse and adhere the bacteria in the adsorbent. In this sealed polyethylene bag, with an irradiation device having a cobalt 60 radiation source,
Gamma ray irradiation was performed at the irradiation dose rate and irradiation time shown in Table 1 below.
【００１４】照射処理後の細孔容積をマイクロメティッ
ク社製のアサップ２４００により、および比表面積を
（株）島津製作所製のフローソーブによりそれぞれ測定
した。また、菌の生存の有無を菌培養法によって、比較
した。その結果、合成吸着剤菌胞子植え付け品のγ線照
射試験の実施例１〜７において、γ線照射の有無によっ
て、水分量、比表面積及び細孔容積には実質的に変化が
なく、しかもγ線照射により菌の胞子は死滅しているこ
とが確認された。結果を表１に示す。The pore volume after the irradiation treatment was measured with an Asap 2400 manufactured by Micrometics, and the specific surface area was measured with a flowsorb manufactured by Shimadzu Corporation. In addition, the presence or absence of bacterium survival was compared by the bacterium culture method. As a result, in Examples 1 to 7 of the γ-ray irradiation test of the synthetic adsorbent fungal spore-implanted products, the water content, the specific surface area and the pore volume did not substantially change depending on the presence or absence of γ-ray irradiation, and γ It was confirmed that the spores of the fungus were killed by the irradiation. The results are shown in Table 1.
【００１５】[0015]
【表１】 [Table 1]
【００１６】実施例８ 市販のスチレン−ジビニルベンゼン共重合体の合成吸着
剤“ダイヤイオンＨＰ２０ＳＳ”（三菱化学（株）製
比表面積５４０ｍ2 ／ｇ、細孔容積１．２９ｍｌ／ｇ、
平均粒子径約１２０μｍ）を水膨潤状態で１００ｍｌ計
量し、ポリエチレン製袋に密封した。この袋に、コバル
ト６０線源を有する照射装置を用いて、１０ｋＧｙのγ
線照射を行った。γ線照射処理前後の合成吸着剤の細孔
容積をマイクロメティック社製のアサップ２４００によ
り、比表面積をマイクロメティック社製のフローソープ
２３００により、それぞれ測定した。その結果、合成吸
着剤のγ線照射試験において、表２に示すように、γ線
照射処理前後における比表面積、細孔容積は実質的に変
化がないことが確認された。これらを用いて、実施例１
と同様に、水分量の測定、菌の胞子存在の経時変化を調
べた。結果を表２に示す。Example 8 Commercially available styrene-divinylbenzene copolymer synthetic adsorbent "Diaion HP20SS" (manufactured by Mitsubishi Chemical Corporation)
Specific surface area 540 m 2 / g, pore volume 1.29 ml / g,
100 ml of an average particle diameter of about 120 μm) was weighed in a water-swelled state and sealed in a polyethylene bag. In this bag, an irradiation device having a cobalt 60 radiation source was used to obtain a γ of 10 kGy.
Irradiation was performed. The pore volume of the synthetic adsorbent before and after the γ-ray irradiation treatment was measured by an Asap 2400 manufactured by Micrometrics, and the specific surface area was measured by a flow soap 2300 manufactured by Micrometrics. As a result, in the γ-ray irradiation test of the synthetic adsorbent, as shown in Table 2, it was confirmed that the specific surface area and the pore volume before and after the γ-ray irradiation treatment did not substantially change. Using these, Example 1
Similarly to the above, the water content was measured and the change over time in the presence of spores of the bacterium was examined. Table 2 shows the results.
【００１７】[0017]
【表２】 [Table 2]
【００１８】実施例９ スチレン−ジビニルベンゼン系吸着剤粒子である“ダイ
ヤイオンＨＰ２０ＳＳ”５ｋｇを大気開放下でポリエチ
レン袋に入れ開口部をヒートシールにより密閉した。こ
の袋に60Ｃｏを線源とする放射線１０ｋＧｙを照射した
後、２４ケ月間室温で保管した。無菌室にてこの袋を開
封し、吸着剤０．２ｇをカビ用シャーレ（ポテトデキス
トロース寒天培地）に接種し、１０日間、２７℃で培養
した。培養シャーレを顕微鏡下で観察したが、Ｐｅｎｉ
ｃｉｌｌｉｕｍ属のカビ（空気中に浮遊しているカビの
一種）の生育は見られず胞子が死滅していることが確認
された。Example 9 5 kg of "Diaion HP20SS", which is a styrene-divinylbenzene-based adsorbent particle, was placed in a polyethylene bag under the atmosphere and the opening was sealed by heat sealing. This bag was irradiated with 10 kGy of radiation having 60 Co as a radiation source and then stored at room temperature for 24 months. The bag was opened in a sterile room, and 0.2 g of the adsorbent was inoculated into a fungal dish (potato dextrose agar medium) and cultured at 27 ° C. for 10 days. The culture petri dish was observed under a microscope.
No growth of mold of the genus Cillium (a type of mold floating in the air) was observed, and it was confirmed that the spores were dead.
【００１９】比較例２ 放射線の照射を行わなかった以外は実施例１０と同様に
して吸着剤を袋内に密封し、２４カ月後、袋より吸着剤
を取り出し、カビ培養シャーレにとり観察したところ、
カビの生育が見られ吸着剤中で胞子が死滅していなかっ
たことが確認された。Comparative Example 2 The adsorbent was sealed in a bag in the same manner as in Example 10 except that radiation was not applied, and after 24 months, the adsorbent was taken out from the bag and observed in a mold culture dish.
Mold growth was observed and it was confirmed that the spores were not killed in the adsorbent.
【００２０】[0020]
【発明の効果】密封状態で放射線照射処理された吸着剤
粒子は、長期に亘って防菌効果があり、且つ、吸着性能
の低下することがない。The adsorbent particles that have been subjected to radiation treatment in a sealed state have a bactericidal effect for a long period of time, and the adsorption performance does not deteriorate.
Claims (7)
粒子を密封包装し、この密封包装した状態の吸着剤粒子
に放射線を照射し、該密封包装状態で吸着剤粒子を保管
する方法。1. A method in which adsorbent particles composed of porous particles of a synthetic polymer are hermetically packaged, the adsorbent particles in the hermetically sealed state are irradiated with radiation, and the adsorbent particles are stored in the hermetically sealed state.
００ｍ2 ／ｇ、細孔容積が０．１〜３ｍｌ／ｇ、粒子径
が５〜１，０００μｍの多孔質粒子である、請求項１記
載の吸着剤粒子の保管方法。2. The adsorbent particles have a specific surface area of 10 to 1,5.
The method for storing adsorbent particles according to claim 1, wherein the adsorbent particles are porous particles having a particle size of 00 m 2 / g, a pore volume of 0.1 to 3 ml / g, and a particle size of 5 to 1,000 μm.
ゼン共重合体である、請求項１記載の吸着剤粒子の保管
方法。3. The method for storing adsorbent particles according to claim 1, wherein the synthetic polymer is a styrene-divinylbenzene copolymer.
放射線である、請求項１に記載の吸着剤粒子の保管方
法。4. The method for storing adsorbent particles according to claim 1, wherein the radiation treatment is radiation with a dose of 5 to 20 kGy.
吸着剤粒子の保管方法。5. The method for storing adsorbent particles according to claim 1, wherein the radiation is gamma rays.
である、請求項５記載の吸着剤粒子の保管方法。6. The method for storing adsorbent particles according to claim 5, wherein the gamma rays are gamma rays having cobalt 60 as a radiation source.
用いて行われることを特徴とする請求項１に記載の吸着
剤粒子の保管方法。7. The method for storing adsorbent particles according to claim 1, wherein the hermetically sealed packaging is performed by using a bag made of a thermoplastic resin film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP8290149A JPH09206361A (en) | 1995-11-28 | 1996-10-31 | How to store adsorbent particles |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP30921395 | 1995-11-28 | ||
| JP7-309213 | 1995-11-28 | ||
| JP8290149A JPH09206361A (en) | 1995-11-28 | 1996-10-31 | How to store adsorbent particles |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH09206361A true JPH09206361A (en) | 1997-08-12 |
Family
ID=26557913
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP8290149A Pending JPH09206361A (en) | 1995-11-28 | 1996-10-31 | How to store adsorbent particles |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH09206361A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012506269A (en) * | 2008-10-24 | 2012-03-15 | フレゼニウス メディカル ケア ドイチュランド ゲゼルシャフト ミット ベシュレンクテル ハフトング | Method and apparatus for sterilization, in particular for sterilization of adsorbers |
| JP2013151822A (en) * | 2012-01-25 | 2013-08-08 | Inamori Co Ltd | Manufacturing method of mildew-proofing tatami facing, and mildew-proofing tatami facing |
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| JPS53128583A (en) * | 1977-04-15 | 1978-11-09 | Kuraray Co Ltd | Process for sterilizing selectively permeable membrane |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2012506269A (en) * | 2008-10-24 | 2012-03-15 | フレゼニウス メディカル ケア ドイチュランド ゲゼルシャフト ミット ベシュレンクテル ハフトング | Method and apparatus for sterilization, in particular for sterilization of adsorbers |
| JP2013151822A (en) * | 2012-01-25 | 2013-08-08 | Inamori Co Ltd | Manufacturing method of mildew-proofing tatami facing, and mildew-proofing tatami facing |
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