JPH09309841A - Preparation for external use for skin - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a preparation for external use for skin which shows aging- preventive activity, gives improvement and preventive effects against skin- roughening symptoms caused by skin diseases and roughening-property liable to have a chippy skin in healthy persons and effectively serves for an oxidative injury for skin by formulating an extract of Imperata cylindrica. SOLUTION: This preparation for external use for skin is obtained by initially extracting dry powder of a rootstalk of Imperata cylindrica under immersing in 60% methanol at room temperature for 10 days. After a solution of extract is filtered, the solution is concentrated with evaporation of methanol to obtain an extract as a dry residue. Next, this residue is formulated with acetostearyl alcohol, squalene, beeswax, reduced lanolin, ethyl parapen, polyoxyethylenesolbitanmonopalmitic acid ester, stearic acid glyceride and perfumes and dissolved under heating and then the solution is added with stirring into an aqueous phase consisting of 1.3-butyleneglycol, polyethylene-glycol and purified water to obtain the objective preparation for external use for skin in a creamy state or the like.

Description

Detailed Description of the Invention

[0001]

TECHNICAL FIELD The present invention relates to an external preparation for skin,
In particular, when combined with cosmetics (including quasi-drugs. The same applies hereinafter), it has an excellent anti-aging effect, and it also causes rough skin symptoms due to various skin diseases such as contact dermatitis and psoriasis, as well as that of healthy people. It has an improving / preventive action against rough skin and rough skin,
In addition, the present invention relates to an external preparation for skin that also exhibits an antioxidant effect that is effective in preventing the oxidation of lipid components of the skin and oxidative damage to the skin.

[0002]

2. Description of the Related Art Aging is progressing in organs of the whole body, and among them, the visible skin, especially the face on which the consciousness is particularly concentrated, is wrinkled or wrinkled.・ The occurrence of freckles and tarmi,
Physical changes such as the loss of firmness and gloss have plagued many middle-aged people in the world, especially women. Up until now, the need for an external anti-aging agent has been screaming,
Since there are many unclear points in the mechanism and definition of aging, in conventional cosmetics, we have selected the method of blending biochemical products such as mucokind and collagen and synthetic polymer products to help retain water. It ’s just However, it has become clear that this alone cannot sufficiently prevent skin aging.

However, in recent years, research on aging has been advanced, and aging is an important factor as a cause of skin aging from a macroscopic viewpoint, and dryness, oxidation, sunlight (ultraviolet rays), etc. are also directly involved in skin aging. Has been listed as a factor. Among these factors, it has become clear that sunlight (ultraviolet ray) plays an important role in a change called photoaging. The face described above is the site where photoaging is most likely to progress in the whole body, but it is also clear that collagen fibers, which are the main matrix components of the dermis, are significantly reduced in photoaged skin. Came.
It has also been suggested that phenomena such as the generation of wrinkles and fine wrinkles and the disappearance of firmness are closely related to the decrease of collagen fibers. As described above, with respect to skin aging, various skin aging factors, in particular, exposure to sunlight (ultraviolet rays) causes a decrease in the proliferative activity of fibroblasts, which are the main cells in the dermis, and the synthetic function of collagen and the like. The turnover speed of collagen etc. also becomes slower. As a result, the skin loses elasticity, wrinkles and sagging increase,
Skin aging progresses.

On the other hand, there are various skin diseases and rough skin as another factor of skin troubles. As an active ingredient for therapeutic agents, skin external preparations, cosmetics, etc. that have an improving / preventive effect against these, amino acids and polysaccharides, lipids, extracted extracts, etc., which have anti-inflammatory effects or have a high moisturizing effect than before, It has been used because of its characteristic use effect. However, recent studies have revealed that proteases are involved in the pathogenesis of various skin diseases. For example, in psoriasis, which is a representative of inflammatory dyskeratosis, high plasminogen activator (PA) activity is observed in the affected epidermis. PA is one of the serine proteases, but Haustein has strong PA in the epidermis of psoriasis
Report the existence of activity (Arch.Klin.Exp.Dermato
L; 234,1969), Fraki and Hopsu-Havu extracted PA activity from psoriatic scales using a highly concentrated salt solution (Arch. Derma).
tol.Res; 256,1976). Also, in pemphigus vulgaris, PA synthesized in epidermal cells in a large amount exists extracellularly.
It has been revealed in an in vitro experimental system that Plasminiogen is converted to Plasmin, which digests intercellular binding substances to store interstitial fluid between cells and form blisters in the epidermis (Morioka S. et al: J.Invest.Derma
tol; 76,1981). Proteases are also considered to play an important role in the normal keratinization process of the epidermis such as stratum corneum formation (Ogawa H., Yoshiike T .: Int. JD.
ermatol; 23, 1984), attempts have been made to use protease inhibitors as agents for improving skin or treating skin diseases.

[0005]

[Means for Solving the Problems] In view of the present situation as described above, the present inventor has conducted diligent studies, and as a result, the extract of Chigaya has an important component of the dermis by acting on fibroblasts in the skin. It has the action of promoting collagen biosynthesis, which is one of the following, and also has the inhibitory activity of trypsin type serine protease, proliferative skin thickening, erythema, dryness,
It was found that skin roughness accompanied by desquamation is extremely effectively improved. Furthermore, it has been found that the extract has an antioxidant power equal to or higher than that of vitamin E. The present inventors have completed the present invention based on the above findings.

That is, the present invention refers to Chigaya (scientific name: Impera
ta cylindrica) is an external preparation for skin characterized by being blended with an extract. Here, the external preparation for skin refers to an agent applied to the skin surface of the human body and the like excluding hair and scalp. Furthermore, according to the present invention, Chigaya (scientific name: Imperata cylindr
The present invention provides an anti-aging agent, a rough skin improving agent, and an antioxidant, which contain an extract of ica) as an active ingredient.

The structure of the present invention will be described in detail below.
Chigaya used in the present invention (scientific name: Imperata cylindric
a) is a perennial herb that is distributed from Hokkaido to Kyushu, Asia, and Africa, and grows in fields, rivers, grasslands, roadsides, etc. The extract used in the present invention can be obtained by immersing or heating the above rhizome with an extraction solvent or refluxing, filtering and concentrating. Further, the extraction solvent used in the present invention may be any solvent that is usually used for extraction, and particularly alcohols such as methanol and ethanol, hydrous alcohols, acetone, and organic solvents such as acetic acid ethyl ester may be used alone or in combination. You can Chigaya is known as one component of a hair-growth / hair-growth agent (Japanese Patent Laid-Open No. 193819/1992), but it has not yet been applied as an external preparation for the skin.

[0008] The blending amount of the cypress extract in the present invention is
When it is used as an anti-aging agent or a rough skin improving agent, it is 0.005 to 20.0% by weight, preferably 0.01 to 10.0% by weight, as a dry product in the total amount of the external preparation. 0.
If it is less than 005% by weight, sufficient antiaging effect and rough skin improving / preventing effect are not exhibited, and if it exceeds 20.0% by weight, formulation is difficult, which is not preferable. Further, even if it is blended in an amount of 10.0% by weight or more, the effect is not so much improved. Further, when the extract of Chigaya is used as an antioxidant, the amount added is generally 0.00001 to 5.0% by weight (as a dried product), preferably 0.00005 to 2.
By adding 0% by weight, safe and excellent antioxidant action is exhibited.

Cosmetics are preferable as an object to be blended with the extract of Chigaya, and various cosmetics, such as creams, which require antioxidant action, rough skin improving action, or anti-aging action.
Lotion, emulsion, hair oil, shampoo, conditioner,
When added to soap, etc., it exhibits excellent effects.

Further, the external preparation for skin of the present invention can be applied as a protease inhibitor. Protease, which is a protease inhibitor, is a general term for enzymes that catalyze the hydrolysis of peptide bonds, and this protease is classified into peptidases and proteinases. The former is from outside the amino terminal or carboxyl terminal of the peptide chain.
It is an enzyme that breaks peptide bonds, and the latter is an enzyme that breaks specific bonds inside peptide chains. The latter proteinases are further roughly classified into four types, serine, cysteine, aspartic acid, and metal, depending on the type of active catalytic group, and each has a specific inhibitor. The protease inhibitor in the present invention is characterized by exhibiting inhibitory activity against serine protease among them.

The external preparation for skin of the present invention contains, in addition to the above-mentioned essential components, components usually used in external preparations for skin such as cosmetics and pharmaceuticals, for example, an aqueous component, an oil component, a powder component, alcohols, a moisturizer, A thickener, an ultraviolet absorber, a whitening agent, a preservative, an antioxidant, a surfactant, a fragrance, a coloring agent, various skin nutritional agents and the like can be appropriately added if necessary.

[0012] In addition, sequestering agents such as disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, gluconic acid, caffeine, tannin, verapamil, licorice extract,
Grabridine, hot water extract of fruits of fire thorns, various crude drugs, drugs such as tocopherol acetate, glycyrrhizic acid, tranexamic acid and its derivatives or salts thereof, vitamin C,
Whitening agents such as magnesium ascorbyl phosphate, glucoside ascorbate, arbutin and kojic acid, and sugars such as glucose, fructose and trehalose can also be appropriately added.

[0013]

Next, the present invention will be described in more detail by way of examples. Note that the present invention is not limited to this. The blending amount is% by weight. Prior to Examples, test methods and results of (1) protease inhibitory action, (2) rough skin improving action, (3) anti-aging action, and (4) antioxidant action of the plant extract of the present invention will be described.

(1) Protease Inhibitory Action The inhibitory activity against plasmin and trypsin was evaluated as two typical serine proteases.

(1-1) Preparation of sample 50 g of dried powder of Rhizoma perforatum was immersed in methanol at room temperature for 1 week, and the extract was concentrated to give methanol extract 1.8.
g was obtained. Dissolve this solid in methanol again and add 1%
A solution was made. The following experiment was performed using this.

(1-2) Measurement of plasmin inhibitory activity The inhibition rate% was determined by the fibrin plate method. Ie Astr
Fibrin plates were prepared according to the method of up et al. (Arch. Biochem .; 40,346, 1952), and the samples prepared as described above were diluted with ethanol to 0.1% and 0.01% and used. The results are shown in Table 1.

(1-3) Measurement of trypsin inhibitory activity Muramatu et al. (J. Biochem .; 58, 21) using casein as a substrate.
4, 1965) and the inhibition rate was calculated. Samples were also diluted to 0.1% and 0.01%, and the results are shown in Table 1.

In addition, as a reference example, ginger (Zingiberac) which is a plant known to be applied to rough skin is already known.
eae) Kunit (scientific name: Curcuma d)
omestica) and wormwood ethanol extract were subjected to the same test as above. Table 1 also shows the results.

[0019]

[Table 1] ──────────────────────────────── Inhibition rate (%) Sample addition concentration ────── ───────── Plasmin trypsin ──────────────────────────────── Chigaya 0.1% 76.3 48.9 0.01% 23.6 19.7 Khunit 0.1% 3.0 0 0.01% 0 0 Wormwood 0.1% 18.6 0 0.01% 5.8 0 ─────────────────────────────── ──

(2) Rough skin improving action (2-1) Actual use test The effect of the external preparation of the present invention applied to the outer skin was evaluated from the improvement rate against rough skin and razor loss, and skin irritation. Using the face of a panel of 50 women suffering from rough skin, of the lotions having the composition (% by weight) shown in Table 2, the sample was placed on one of the left and right cheeks, and the other cheek was a control twice a day for 2 weeks. After application, the skin condition after that was visually evaluated. Further, a lotion of 30 men who lost razors was applied with a lotion having the composition shown in Table 2 immediately after shaving, and the effect on razor loss was evaluated. Each criterion was as follows. As a sample, as the product of the present invention,
2 kinds of different concentrations of the methanol extract of Chigaya,
As comparative products, the Kungyit (Curcuma domestica) of the Zingiberaceae family, which is a plant already known to be applied to rough skin, and the Lempyan (Lemp) family of the Zingiberaceae family, are known.
uyang, scientific name: Zingiber aromaticum Mal.) blended with a methanol extract was used. Table 3 shows the results.
Shown in

Criteria for the improvement effect on rough skin: Remarkable effect: symptom disappeared. Effective: those with reduced symptoms. Somewhat effective: Somewhat weakened symptoms. Ineffective: No change in symptoms.

Criteria for Improvement Effect on Razor Loss Remarkable effect: Razor loss disappeared. Effective: Razor loser weakened. Somewhat effective: Razor losing slightly weakened. Invalid: No change in razor loss.

Improvement effect on rough skin and razor loss ⊚: 80% or more of the rate (effective rate) at which the test subject is markedly effective, effective and slightly effective. ：: The proportion (effective rate) at which the test subject shows a remarkable effect, an effective effect and a somewhat effective effect is 50% or more and less than 80%. Δ: The ratio (effective rate) at which the test subject showed remarkable, effective, and somewhat effective (effective rate) was 30% or more and less than 50%. ×: The ratio (effective rate) at which the subject exhibited significant, effective, and somewhat effective (effective rate) was less than 30%.

Skin irritation ⊚: 0% of the subjects recognized tingling on the skin. ：: The proportion of subjects who felt tingling on the skin was less than 5%. Δ: The ratio of subjects who felt burning on the skin was less than 10%. ×: The proportion of subjects who felt burning on the skin was 10% or more.

[0025]

[Table 2] ──────────────────────────────── Inventive product Comparative product Test product ──────── ─────── 2-1 2-2 2-1 2-2 ──────────────────────────────── ─ Tigaya methanol extract 1.0 0.5 − − Khunit methanol extract − − 1.0 − Rempuyan methanol extract − − − 1.0 Glycerin 1.0 1.0 1.0 1.0 1,3-butylene glycol 4.0 4.0 4.0 4.0 Ethanol 7.0 7.0 7.0 7.0 Polyoxyethylene (20 Mol) Oleyl alcohol 0.5 0.5 0.5 0.5 Purified water Residual Residual Residual ──────────────────────────────────

[0026]

[Table 3] ──────────────────────────────── Comparative product of the present invention Sample ──────── ─────── 2-1 2-2 2-1 2-2 ──────────────────────────────── ─ Rough skin improvement effect ◎ ◎ △ △ Razor loss improvement effect ◎ ◎ ◎ △ △ Skin irritation ◎ ◎ ○ △ ─────────────────────────── ──────

As is clear from Table 3, the samples of the present invention containing the extract of Tigaya extract showed a better effect on rough skin and razor loss than the samples of the comparative products, and also showed skin irritation. I couldn't do it.

(2-2) Actual Use Test by Replica Method Using the lotions of the products 2-1 and 2-2 of the present invention and the comparative products 2-1 and 2-2, a skin roughness improving effect test was conducted on a human body panel. . That is,
A replica of the skin surface of a healthy female (face) was taken using a replica method using a millisin resin, and a microscope (1)
7 times). Based on the criteria shown in Table 4, the rough skin evaluation is 1 or 2 based on the state of the skin pattern and the peeling state of the stratum corneum.
Using 20 people judged to be rough (skin rough panel), the lotions of the invention products 2-1 and 2-2 and the comparative products 2-1 and 2-2 were applied to the left and right sides of the face half and twice a day for 2 weeks. did. Two weeks later, the skin condition was observed again according to the above-mentioned replica method, and evaluated according to the criteria shown in Table 4. The results are shown in Table 5.

[0029]

[Table 4] ───────────────────────────────── Scores Standards of scores ──────── ─────────────────────────── 1 Disappearance of skin crevices, cuticles, and wide-ranging horny layer are observed. 2 Unsmooth skin groove and crust, and horny turn of the stratum corneum. 3 There are pits and ridges, but they are flat. 4 Clear skin grooves and ridges. 5 Crusts and ridges are clear and well organized. ─────────────────────────────────

[0030]

[Table 5] ───────────────────────────────── Replica evaluation Inventive product 2-1 Inventive product 2- 2 Comparative product 2-1 Comparative product 2-2 ───────────────────────────────── 1 0 0 1 1 1 2 0 0 3 2 3 3 4 4 14 11 4 9 12 2 6 5 8 8 4 0 0 ──────────────────────────────── ──

As can be seen from Table 5, the lotion of the product of the present invention had a remarkable effect of improving rough skin as compared with the lotion of the comparative product.

(3) Anti-aging action (3-1) Evaluation of action of human skin fibroblasts on type I collagen production ability Using human skin fibroblasts (hereinafter, cells), type I collagen biosynthesis ability of cells The effect of the extract of Spodoptera litura on was evaluated. Preparation of the extract of Cyperus notifolia was carried out in the same manner as in the case of the evaluation of "(1) Protease inhibitory action". That is, 50 g of dry powder of Rhizoma rhizome was immersed in methanol at room temperature for 1 week, and the extract was concentrated to obtain 1.8 g of a methanol extract (hereinafter, Tigaya extract).

Cells were seeded at 20000 cells / well in a 96-well plate for cell culture (Corning: 25860).
R containing 10% fetal bovine serum (hereinafter referred to as FBS)
After culturing in ITC80-7 medium for 48 hours, 0.5% F
The RITC80-7 medium containing BS (hereinafter referred to as medium) was replaced. At that time, the extract of Cyperus edulis dissolved in dimethylsulfoxide was added to the medium. The concentration of dimethylsulfoxide in the medium was set to be 0.5%, and the concentration of the extract of Tsuchiya was 1 ppm. After the medium was replaced with the medium containing the extract of Tsuchiya extract, the culture was carried out for 48 hours. After the completion of the culture, the culture supernatant was collected to measure the type I collagen biosynthesis ability, and the cells were collected to measure the cell amount.
The type I collagen biosynthesis ability of cells depends on the C-terminal peptide (Procollage) of type I procollagen secreted in the culture supernatant.
n type IC-peptide: Abbreviated as PIP. ) Was evaluated by measuring the amount. Specifically, "Procollagen type IC-
peptide (PIP) measurement kit (Takara Shuzo Co., Ltd .: MK00
1) ”. The amount of cells was estimated by the amount of DNA in the cells, and the amount of DNA was measured using Hoechst 33258 reagent according to the method of Cesar Lsbarca et al. (Analytical Biochemisty, 102, 344-352, (1980)).

The results are shown in Table 6. Although the amount of DNA showed a slight increasing tendency, it was not a significant change,
A significant increase in the amount of PIP was observed. As described above, it was confirmed that the extract of Porphyra chinensis has an effect of promoting type I collagen biosynthesis without affecting cell proliferation at an extremely low concentration of 1 ppm.

[0035]

[Table 6] ──────────────────────────────── Tigaya extract concentration DNA amount (μg / well) PIP amount ( ng / μg DNA) ──────────────────────────────── 0ppm 0.404 ± 0.038 425.62 ± 66.20 1ppm 0.434 ± 0.062 611.55 ± 72.07 ────────────────────────────────

(3-2) Actual Use Test The following use tests were carried out on the cream formulations obtained in Example 3-1 and Comparative Example 3-1 having the formulations shown in Table 7. Table 8 shows the results.

[0037]

[Table 7] ───────────────────────────────── Example 3-1 Comparative Example 3-1 ─── ────────────────────────────── (1) Cetostearyl alcohol 3.5 3.5 3.5 (2) Squalane 40.0 40 0.0 (3) Beeswax 3.0 3.0 (4) Reduced lanolin 4.0 4.0 (5) Ethylparaben 0.3 0.3 (6) Polyoxyethylene (20) sorbitan monopalmitate 2. 0 2.0 (7) Stearic acid monoglyceride 2.0 2.0 (8) Chigaya extract 0.5- (9) Sodium N-stearoylglutamate 0.5 0.5 (10) Perfume 0.03 0.03 (11) 1,3-Butylene glycol 5.0 5.0 (12) Polyethylene glycol 1500 5.0 5.0 (13) Purified water Residual residue ──────────── ─────────────────────

Production Method The raw materials (1) to (10) shown in Table 7 above are melted by heating (oil phase). On the other hand, (11) and (12) were dissolved in purified water (13) and kept at 70 ° C. (aqueous phase). Then, the oil phase was added to the aqueous phase while stirring. Then, the mixture was treated with a homomixer to make the emulsified particles fine, then stirred, and then rapidly cooled with stirring to obtain a desired cream.

Use test Randomly extracted healthy adult females 25-57 years of age 1
00 subjects were used, and after applying each cosmetic to the facial skin for 1 day every day, the improvement effect on (a) skin firmness / talmi and (b) improvement effect on wrinkles / small wrinkles were examined respectively. .

(A) Effect of improving skin firmness The condition of the skin was visually observed and evaluated based on the following evaluation criteria. (Evaluation Criteria) A: Very improved. B: Improved. C: No change. D: A little noticeable. E: It became noticeable.

(B) Improvement effect on wrinkles and fine wrinkles The condition of the skin was visually observed and evaluated based on the following evaluation criteria. (Evaluation criteria) A: It disappeared neatly. B: It became a little less noticeable. C: No change. D: Increased a little. E: Increased.

[0042]

[Table 8] ─────────────────────────────────── Effect on firmness / talmi, wrinkles, and small wrinkles (Person) Effect on (Person) ───────────── ───────────── A B C C D E A B C C D E ─────── ───────────────────────────── Example 3-1 62 25 13 0 0 37 42 21 0 0 Comparative example 3-1 3 15 76 4 2 0 10 81 8 1 ───────────────────────────────────

As is clear from the results shown in Table 8, when the cosmetics obtained in Example 3-1 were used, Comparative Example 3-1
It was revealed that the firmness and tarmi of the skin was remarkably improved as compared with the case of using the cosmetic obtained in 1. and it was also extremely effective against wrinkles and fine wrinkles.

(4) Antioxidant action (4-1) Preparation of sample Preparation Example 1 (methanol extract) To 1 kg of dried cypress root, 20 kg of 60% methanol was added and extracted by immersing at room temperature for 10 days for extraction. After filtering the liquid, the methanol was concentrated to leave the extract as a dry residue.
0 g was obtained.

Preparation Example 2 (Chloroform Extract) 9 kg of chloroform was added to 1 kg of dried dried Cyper root, and 5
After refluxing at 0 ° C. for 8 hours for extraction and filtering the extract,
Chloroform is concentrated and the extract is 20 g as a dry residue.
Obtained.

Preparation Example 3 (Purified Methanol Extract) 10 kg of methanol was added to 1 kg of dried dried sardine root, and the mixture was refluxed and extracted for 8 hours by reflux boiling extraction. The extract was filtered, and the filtrate was concentrated. 10 g of extract was obtained.

(4-2) Test method and test results 1.0 mg methyl linoleate (manufactured by Tokyo Kasei Kogyo Co., Ltd.) / Ml 1.0 ml of acetone solution and 0.01 mg / ml of the cypress extract prepared in Preparation Example 1 After 0.1 ml of a methanol solution or 0.1 mg / ml methanol solution was dispensed to a tester with a screw lid and dried, the oxidized group was treated at 50 ° C. for 4 hours and the non-oxidized group was stored at 4 ° C. After treatment, non-aqueous TB
Add 3.0 ml of reagent A (0.12% 2-thiobarbituric acid (Wako Pure Chemical Industries, Ltd.) / 0.50% triethylamine (Tokyo Chemical Industry Co., Ltd.) / Acetic acid), and add 1 at 95 ° C.
Allowed to react for hours. Immediately after the reaction, the mixture was cooled with water, the absorbance at 532 nm was measured, and the oxidation inhibition rate was calculated.

[0048]

[Equation 1] Oxidation inhibition rate (%) = [1- (As50-As4) /
(Ac50-Ac4)] × 100

Ac50: control Absorbance at 50 ° C. heating. Ac4: control Absorbance stored at 4 ° C. As50: Absorbance of sample heated at 50 ° C. As4: Absorbance of sample stored at 4 ° C.

As a control, vitamin E (DL-α-tocopherol, manufactured by Tokyo Kasei Kogyo Co., Ltd.) methanol solution and BHT (butyl hydroxytoluene: Wako Pure Chemical Industries Co., Ltd.) methanol solution were added in place of the cypress extract. The same operation was performed for the ones that were made. The results are shown in Table 9.

[0051]

[Table 9] ──────────────────────────────── Sample No. Antioxidant Compounding amount (mg) Oxidation inhibition rate (%) ──────────────────────────────── 4-1 Chigaya extract 0.001 61.9 4-2 Chigaya extract 0.01 95.6 ──────────────────────────────── 4-3 Vitamin E 0.001 42.4 4-4 Vitamin E 0.01 82.4 4-5 BHT 0.001 97.3 ─────────────────────────────────

As shown in Table 9, Tigaya extract shows an antioxidant effect superior to that of vitamin E. Further, when the concentration of Tigaya extract is increased, it shows a remarkable antioxidant action equivalent to BHT.

Example 1 Cream (formulation) Stearic acid 5.0% by weight Stearyl alcohol 4.0 Isopropyl myristate 18.0 Glycerin monostearate 3.0 Propylene glycol 10.0 Chigaya methanol extract 0.01 Caustic potash 0 .2 Sodium bisulfite 0.01 Preservative Suitable amount Perfume Suitable amount Ion-exchanged water Residual (production method) Propylene glycol, Tigaya methanol extract and caustic potash were added to ion-exchanged water and dissolved, and heated to 7
Keep at 0 ° C. (aqueous phase). Mix other ingredients, heat and melt.
Keep at 0 ° C. (oil phase). The oil phase is gradually added to the water phase, and after the addition is completed, the temperature is maintained for a while to cause a reaction. Then, it is uniformly emulsified with a homomixer and cooled to 30 ° C. with thorough stirring.

Example 2 Cream (formulation) Stearic acid 2.0% by weight Stearyl alcohol 7.0 Hydrogenated lanolin 2.0 Squalane 5.0 2-Octyldodecyl alcohol 6.0 Polyoxyethylene (25 mol) cetyl alcohol ether 3.0 Glycerin monostearate 2.0 Propylene glycol 5.0 Chigaya ethanol extract 0.05 Tranexamic acid 0.2 Ethylparaben 0.3 Fragrance suitable amount Ion-exchanged water Residual (production method) Add propylene glycol to ion-exchanged water ,
Heat and maintain at 70 ° C. (aqueous phase). The other components are mixed, melted by heating and kept at 70 ° C. (oil phase). The oil phase is added to the aqueous phase to carry out preliminary emulsification, and the mixture is uniformly emulsified with a homomixer and then cooled to 30 ° C. with thorough stirring.

Example 3 Cream (Formulation) Solid paraffin 5.0% by weight Beeswax 10.0 Vaseline 15.0 Liquid paraffin 41.0 Glycerin monostearate 2.0 Polyoxyethylene (20 mol) sorbitan monolaurate 2 0.0 Soap powder 0.1 Chigaya acetone extract 0.05 Sodium hydrogen sulfite 0.03 Ethylparaben 0.3 Perfume proper amount Ion-exchanged water Residual (production method) Add soap powder to ion-exchanged water and heat to 7
Keep at 0 ° C. (aqueous phase). Mix other ingredients, heat and melt.
Keep at 0 ° C. (oil phase). The oil phase is gradually added to the aqueous phase while stirring to carry out the reaction. After the reaction is completed, the mixture is uniformly emulsified with a homomixer, and after emulsification, the mixture is cooled to 30 ° C. with thorough stirring.

Example 4 Emulsion (formulation) Stearic acid 2.5% by weight Cetyl alcohol 1.5 Vaseline 5.0 Liquid paraffin 10.0 Polyoxyethylene (10 mol) monooleate 2.0 Polyethylene glycol 1500 3. 0 Triethanolamine 1.0 Carboxyvinyl polymer 0.05 (trade name: Carbopol 941, BFGoodrich Chemical company) Ethyl acetate extract of Tigaya acetate 0.01 Sodium hydrogen sulfite 0.01 Ethylparaben 0.3 Perfume Suitable amount Ion-exchanged water Residual (manufacturing method) The carboxyvinyl polymer is dissolved in a small amount of ion-exchanged water (phase A). Polyethylene glycol 1500 and triethanolamine are added to the remaining ion-exchanged water, dissolved by heating, and kept at 70 ° C. (aqueous phase). The other components are mixed, melted by heating and kept at 70 ° C. (oil phase). The oil phase is added to the water phase, pre-emulsification is performed, phase A is added, and the mixture is uniformly emulsified with a homomixer. After the emulsification, the mixture is cooled to 30 ° C. while stirring well.

Example 5 Emulsion (Formulation) Microcrystalline wax 1.0% by weight Beeswax 2.0 Lanolin 20.0 Liquid paraffin 10.0 Squalane 5.0 Sorbitan sesquioleate 4.0 Polyoxyethylene (20 mol) Sorbitan monooleate 1.0 Propylene glycol 7.0 Ciguay butanol extract 5.0 Tranexamic acid 1.0 Sodium hydrogen sulfite 0.01 Ethylparaben 0.3 Perfume proper amount Ion-exchanged water Residual (production method) Propylene to ion-exchanged water Add glycol,
Heat and maintain at 70 ° C. (aqueous phase). The other components are mixed, heated and melted and kept at 70 ° C. (oil phase). While stirring the oil phase, the aqueous phase is gradually added thereto, and the mixture is uniformly emulsified with a homomixer. After emulsification, cool to 30 ° C. while stirring well.

Example 6 Jelly (formulation) 95% ethyl alcohol 10.0 wt% dipropylene glycol 15.0 polyoxyethylene (50 mol) oleyl alcohol ether 2.0 carboxyvinyl polymer 0.05 (trade name: Carbopol 940, BFGoodrich Chemical company) Caustic soda 0.15 L-arginine 0.1 Chigaya 50% methanol aqueous solution extract 0.01 Sodium hydrogen sulfite 0.01 Ethylparaben 0.3 Perfume proper amount Ion-exchanged water Residue (production method) For ion-exchanged water Carbopol 940 is uniformly dissolved, while 50% methanol extract of Tigaya 50% aqueous solution and polyoxyethylene (50 mol) oleyl alcohol ether are dissolved in 95% ethanol and added to the aqueous phase. Next, after adding other components, the mixture is neutralized with caustic soda and L-arginine to increase the viscosity.

Example 7 Jelly (formulation) (Phase A) Ethyl alcohol (95%) 10.0% by weight Polyoxyethylene (20 mol) Octyldodecanol 1.0 Pantothenyl ethyl ether 0.1 Chigaya methanol extract 1 .5 Methylparaben 0.15 (Phase B) Potassium hydroxide 0.1 (Phase C) Glycerin 5.0 Dipropylene glycol 10.0 Sodium hydrogen sulfite 0.03 Carboxyvinyl polymer 0.2 (trade name: Carbopol 940, BFGoodrich Chemical compan
y) Purified water Residual (production method) Phases A and C are uniformly dissolved,
Add phases and solubilize. Next, after adding the phase B, filling is performed.

Example 8 Pack (Formulation) (Phase A) Dipropylene glycol 5.0% by weight Polyoxyethylene (60 mol) hydrogenated castor oil 5.0 (Phase B) Tigaya acetone extract 0.01 Olive oil 5.0 Tocopherol acetate 0.2 Ethylparaben 0.2 Perfume 0.2 (Phase C) Sodium hydrogen sulfite 0.03 Polyvinyl alcohol 13.0 (Saponification degree 90, degree of polymerization 2,000) Ethanol 7.0 Purified water Residual (manufacturing method ) A phase, B phase, and C phase are uniformly dissolved,
Add phase B to phase and solubilize. Next, this is added to the C phase and then filled.

Example 9 Solid Foundation (Formulation) Talc 43.1% by weight Kaolin 15.0 Sericite 10.0 Zinc white 7.0 Titanium dioxide 3.8 Yellow iron oxide 2.9 Black iron oxide 0.2 Squalane 8 0.0 isostearic acid 4.0 POE sorbitan monooleate 3.0 isocetyl octanoate 2.0 chigaya ethanol extract 1.0 preservative appropriate amount perfume appropriate amount (manufacturing process) Talc to black iron oxide powder components are thoroughly mixed with a blender. Then, an oily component of squalane to isocetyl octoate, an extract of Chigaya ethanol, a preservative and a fragrance are added, and the mixture is well kneaded and then filled into a container and molded.

Example 10 Emulsified Foundation (Prescription) (Powder Part) Titanium Dioxide 10.3% by Weight Sericite 5.4 Kaolin 3.0 Yellow Iron Oxide 0.8 Bengala 0.3 Black Iron Oxide 0.2 ( Oil phase) Decamethylcyclopentasiloxane 11.5 Liquid paraffin 4.5 Polyoxyethylene-modified dimethylpolysiloxane 4.
0 (Aqueous phase) Purified water 50.0 1,3-Butylene glycol 4.5 Chigaya methanol extract 1.5 Sorbitan sesquioleate 3.0 Preservative proper amount Perfume proper amount (Production method) After heating and stirring the aqueous phase, it is sufficient. Then, the powder portion which has been mixed and pulverized is added to the mixture and homomixed. Further, the oil phase mixed by heating is added, and the mixture is treated with a homomixer. Then, a fragrance is added with stirring, and the mixture is cooled to room temperature.

[0063]

As described above, the external preparation for skin of the present invention has an excellent antiaging effect. That is, with the exposure to sunlight (ultraviolet rays), the proliferation activity of fibroblasts, which are the main cells in the dermis, and the synthetic function of collagen, etc. are reduced, and the damage to the skin caused by the suppression is suppressed and the damage received is restored. This may help to maintain and improve elastic, firm skin without wrinkles or sagging. At the same time, it also has an excellent effect of improving rough skin, and has an excellent effect of improving and preventing various skin diseases, rough skin, rough skin, and the like. Furthermore, since it also has an excellent antioxidant effect, it can be effectively protected against oxidation of skin lipid components, oxidative damage to the skin, and the like by being incorporated into cosmetics to protect the skin.

 ─────────────────────────────────────────────────── ─── Continuation of front page (72) Inventor Motoji Wada 1050 Shinba-cho, Kohoku-ku, Yokohama-shi, Kanagawa Inside Shiseido Daiichi Research Center Co., Ltd. Banchi Co., Ltd. Shiseido Daiichi Research Center

Claims (5)

[Claims] 1. Chigaya (scientific name: Imperata cylindrica)
An external preparation for skin characterized by containing the extract of. 2. Chigaya (scientific name: Imperata cylindrica)
An anti-aging agent, characterized by comprising an extract of the above as an active ingredient. 3. Chigaya (scientific name: Imperata cylindrica)
An agent for improving skin roughness, which comprises the extract of as an active ingredient. 4. Chigaya (scientific name: Imperata cylindrica)
An antioxidant, characterized in that the extract is an active ingredient. 5. The blended amount of Chigaya extract in terms of dry matter,
The agent according to any one of claims 1 to 4, which is 0.00001 to 20.0% by weight.