JPH09501185A - 脂質溶解性酵素の活性の蛍光測定 - Google Patents

脂質溶解性酵素の活性の蛍光測定

Info

Publication number
JPH09501185A
JPH09501185A JP7530054A JP53005495A JPH09501185A JP H09501185 A JPH09501185 A JP H09501185A JP 7530054 A JP7530054 A JP 7530054A JP 53005495 A JP53005495 A JP 53005495A JP H09501185 A JPH09501185 A JP H09501185A
Authority
JP
Japan
Prior art keywords
glycerol
lipase
fluorescence
activity
fluorophore
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP7530054A
Other languages
English (en)
Japanese (ja)
Inventor
ヘルメッテル，アルビン
デューク，マルチン
パルタオフ，フリッツ
Original Assignee
プローゲン ビオテヒニーク ゲーエムベーハー
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by プローゲン ビオテヒニーク ゲーエムベーハー filed Critical プローゲン ビオテヒニーク ゲーエムベーハー
Publication of JPH09501185A publication Critical patent/JPH09501185A/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C229/00—Compounds containing amino and carboxyl groups bound to the same carbon skeleton
    • C07C229/02—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton
    • C07C229/04—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated
    • C07C229/06—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having only one amino and one carboxyl group bound to the carbon skeleton
    • C07C229/18—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having only one amino and one carboxyl group bound to the carbon skeleton the nitrogen atom of the amino group being further bound to carbon atoms of six-membered aromatic rings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/44—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving esterase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C2603/00—Systems containing at least three condensed rings
    • C07C2603/02—Ortho- or ortho- and peri-condensed systems
    • C07C2603/40—Ortho- or ortho- and peri-condensed systems containing four condensed rings
    • C07C2603/42—Ortho- or ortho- and peri-condensed systems containing four condensed rings containing only six-membered rings
    • C07C2603/50—Pyrenes; Hydrogenated pyrenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
JP7530054A 1994-05-19 1995-05-19 脂質溶解性酵素の活性の蛍光測定 Pending JPH09501185A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AT1029/94 1994-05-19
AT0102994A ATA102994A (de) 1994-05-19 1994-05-19 Fluoreszenzbestimmung der aktivität lipolytischer enzyme
PCT/EP1995/001919 WO1995032181A1 (de) 1994-05-19 1995-05-19 Fluoreszenzbestimmung der aktivität lipolytischer enzyme

Publications (1)

Publication Number Publication Date
JPH09501185A true JPH09501185A (ja) 1997-02-04

Family

ID=3505141

Family Applications (1)

Application Number Title Priority Date Filing Date
JP7530054A Pending JPH09501185A (ja) 1994-05-19 1995-05-19 脂質溶解性酵素の活性の蛍光測定

Country Status (4)

Country Link
EP (1) EP0710225A1 (de)
JP (1) JPH09501185A (de)
AT (1) ATA102994A (de)
WO (1) WO1995032181A1 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20020187484A1 (en) * 2000-11-27 2002-12-12 Jon Thorson Molecular break lights probes for detecting nucleotide cleavage
WO2009111439A2 (en) 2008-03-04 2009-09-11 The Trustees Of The University Of Pennsylvania In vivo detection of phospholipase activation
US20180179574A1 (en) * 2015-09-03 2018-06-28 The University Of Chicago Systems and methods for characterization of hypertriglyceridemia
CN119612502B (zh) * 2025-01-21 2025-10-10 吉林大学 一种两亲性石墨烯量子点分散液及其制备方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI63064C (fi) * 1980-04-09 1983-04-11 Ksv Chemicals Oy Foerfarande foer fluorometrisk bestaemning av aktiviteten av fettspjaelkande enzymer och medel foer att genomfoera foerfarandet

Also Published As

Publication number Publication date
ATA102994A (de) 1995-03-15
WO1995032181A1 (de) 1995-11-30
EP0710225A1 (de) 1996-05-08

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