JPH0959143A - Dermal preparation for external use containing 4-hydroxythiophenol - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain a dermal preparation for external use excellent in beautifying and whitening actions. SOLUTION: This dermal preparation for external use comprises 4- hydroxythiophenol as an active ingredient and further a liquid oil such as squalane or jojoba oil, a solid oil such as beeswax or cetyl alcohol, various activators, a humectant such as glycerol or 1,3-butylene glycol or various agents are added thereto and formulated into the form such as a lotion, a cream, a milky lotion or a pack. The 4-hydroxythiophenol has stronger beautifying and whitening actions than those of kojic acid, arbutin, etc., and is extremely effectively used as the active ingredient of the dermal preparation for external use.

Description

Detailed Description of the Invention

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a substance effective for whitening.

[0002]

2. Description of the Related Art Many substances having a whitening effect are already known. Commercialized products include kojic acid and arbutin. However, there were problems such as a weak effect.

[0003]

SUMMARY OF THE INVENTION An object of the present invention is to provide a skin external preparation which is safe when applied to the skin and has a large whitening effect.

[0004]

[Means for Solving the Problems] In order to solve the above-mentioned problems, the present inventors have screened and examined various substances and examined those which are useful as cosmetics. as a result,
When various experiments were conducted focusing on 4-hydroxythiophenol, it was found that the whitening effect was strong.

That is, it is a skin external preparation containing 4-hydroxythiophenol.

This substance is used as a raw material for other cosmetics such as liquid oils such as squalane and jojoba oil, solid oils such as beeswax and cetyl alcohol, various activators, humectants such as glycerin and 1,3 butylene glycol and various chemicals. It can be added to prepare various external forms of the skin external preparation. For example, a lotion, a cream, a milky lotion, a pack, or the like may be used depending on the purpose.

[0007]

EXAMPLE An example of an actual usage will be described below, but the present invention is not limited to this example.

Example-1 Lotion Olive oil 0.5 4-Hydroxythiophenol 0.2 Polyoxyethylene (20E.0) sorbitan monostearate 2.0 Polyoxyethylene (60E.0) hydrogenated castor oil 2.0 Ethanol 10.0 1.0% sodium hyaluronate aqueous solution 5.0 Purified water 80.3

Example-2 Cream A Squalane 20.0 Oleip Oil 2.0 Mink Oil 1.0 Jojoba Oil 5.0 Beeswax 5.0 Cetostearyl Alcohol 2.0 Glycerin Monostearate 1.0 Sorbitan Monostearate 2 0.0 4-Hydroxythiophenol 0.1 B Purified water 48.8 Polyoxyethylene (20E.0) sorbitan monostearate 2.0 Polyoxyethylene (60E.0) hydrogenated castor oil 1.0 Glycerin 5.01 0.0% aqueous solution of sodium hyaluronate 5.0 Methyl paraoxybenzoate 0.1 Weigh A and B respectively, warm to 70 ° C., gradually add A to B with stirring, then slowly stir 30
Cooled to ° C.

Inhibition of tyrosinase activity (Test method) 30 mM sodium phosphate buffer (pH
6.8) 0.9 ml, 0.05% dopa aqueous solution 1.0 m
l, 0.1 ml of a dimethylsulfoxide solution of a sample, and 0.9 ml of purified water were placed in a screw vial, and heated in a constant temperature water bath at 25 ° C for 5 minutes or more. Tyrosinase solution (Si
gma, mushroom-derived, 914 units / m
l) 0.1 ml was added, the mixture was placed in a cell kept at a constant temperature of 25 ° C., and the absorbance was measured at 475 nm for 15 minutes every 15 seconds after 30 seconds. As a control, dimethylsulfoxide was added instead of the above sample solution, and the measurement was performed in the same manner. The concentration was tested in several steps. (Calculation formula) Tyrosinase activity inhibition rate (%) = (BA) / B × 10
0 where A: slope of absorbance of sample B: slope of absorbance of control

[0011]

[Table 1]

B-16 melanoma cell test A sample was added to Eagle's MEM medium to a predetermined concentration and filtered through a sterilization filter.
% So that the pH was adjusted to 7.6 ± 0.1 with sodium hydrogen carbonate, and 6 ml was dispensed into a petri dish, and the B-16 melanoma cell suspension (1 × 10 ⁶ cells) was added.
1 / ml), 5% CO ₂ , 95% a
The cells were cultured under ir conditions at 37 ° C for 3 days. In addition, exchange the medium (Eag containing 10% fetal bovine serum containing the above sample)
lesMEM medium) and cultured for 3 days. (At this time, cell proliferation was determined.) The cells were peeled off, centrifuged, the cells were collected, and the whiteness was visually determined. Brightness Same as blank ± Slight whitening tendency + Clear whitening tendency ++ Strong whitening tendency +++ Cell proliferation 80% or more of blank A 60-80% of blank B 30-60% of blank C 30% of blank Below D

[0013]

[Table 2]

Use Test Eight women's faces were divided into right and left sides, one of which was used as an example and the other was used as a comparative example once or more daily, and a questionnaire was conducted after 3 months. In the comparative example, the production example was changed to water from the example. (Comparative Examples 1 and 2) The criteria are as follows, and the results of the questionnaire are summarized in the table below. Example is much better 3 Example is much better 2 Example is slightly better 1 No difference 0 Comparative example is slightly better -1 Comparative example is much better -2 Comparative example Is much better -3

[0015]

[0016]

[Effect] 4-Hydroxythiophenol has a stronger whitening effect than kojic acid and the like, and is very effective as an active ingredient of a skin external preparation.

Claims (1)

[Claims] 1. An external preparation for skin containing 4-hydroxythiophenol.