JPH10139681A - Aqueous emulsified nutritional composition and its use - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an aqueous emulsion nutritional composition having improved bitterness, excellent in discoloration when being heated, and long term preservation by using L-arginine and a peptide containing glutamine in combination and regulating the pH of the liquid to a specific region. SOLUTION: This aqueous emulsion nutritional composition includes (A) L-arginine and (B) a peptide containing glutamine, and preferably (C) water- insoluble dietary fiber, (D) middle chain fatty acid triglyceride and (E) a nucleic acid, and the pH of the composition is regulated to 5.5-7.0. The composition preferably includes 0.5-1.5wt.% component A, 0.5-2.0wt.% component B, 0.3-0.8wt.% component C, 0.1-1.5wt.% component D and <=0.3wt.% component E, and the pH of the composition is regulated by citric acid.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

TECHNICAL FIELD The present invention relates to an aqueous emulsion nutritional composition containing a specific component, and an oral and enteral nutritional composition containing the same as an active ingredient. More specifically, the present invention relates to a nutritional composition which is excellent in improving palatability at the time of oral administration and preventing coloring when blended into a product. The present invention also relates to an oral and enteral nutritional composition containing the aqueous emulsion nutrition composition as an active ingredient.

[0002]

2. Description of the Related Art Conventionally, oral administration, enteral nutrition administration, central parenteral nutrition administration, and the like have been adopted as nutritional supplementation methods before and after surgery. In recent years, in particular, a method of administering an enteral nutrient from the mouth or directly to the intestine from the viewpoint of physiological aspects and atrophy of the gastrointestinal mucosa has been attracting attention because the gastrointestinal tract is not used for nutritional administration through a central vein or the like. ing. The administration of these enteral nutrients allows the ingestion of nutrients such as proteins, lipids, carbohydrates, vitamins, and minerals, which greatly contributes to the treatment of diseases. Further, recently, for the purpose of aggressive treatment, formulations for further adding various active ingredients to the five major nutrients have been studied. Examples of aggressive treatment include those that activate immunologically and protein metabolism to prevent infectious diseases and to heal wounds at an early stage. In particular, L-arginine and glutamine, which are non-essential amino acids, have been studied. One of these reasons is
L-arginine is said to have effects such as promotion of polyamine, protein synthesis, enhancement of host defense mechanism by enhancement of cellular immune function, and promotion of secretion of various hormones.
Glutamine is said to have an effect of improving energy sources such as intestinal mucosal cells and the metabolic system of proteins (Sur
gerry 108, 331-337, author A.
Barbul et al. And Nutrition Vol. 4, No. 4, pp. 261-268; A. Newsholm
e).

[0003] In the conventional oral administration of a nutritional composition containing L-arginine or glutamine or a glutamine-containing peptide, there is no nutritional composition that is balanced and has improved palatability. In the enteral administration of the nutritional composition, it is known that if the osmotic pressure is too high, it causes symptoms such as diarrhea when ingested in the intestine.
A balanced nutritional composition comprising L-arginine or glutamine or a glutamine-containing peptide,
No nutritional composition has improved osmotic pressure. Conventionally, L-
When arginine and glutamine are blended for the above-mentioned effects, there is a problem in that bitterness is remarkably exhibited, and L-arginine,
When heat treatment such as sterilization is performed to improve the bitterness of amino acids such as glutamine or the like, or to add a carbohydrate as a nutrient and preserve as a liquid nutrition composition, a reaction with aminocarbonyl occurs, and the product is remarkable. There is a problem that coloring occurs and the commercial value is impaired. In addition, when glutamine is blended as a liquid nutritional composition, there is a problem that glutamine is decomposed and disappears during the heat treatment as described above. That is, a nutritional composition capable of adding L-arginine, a glutamine-containing peptide and a carbohydrate and blending in a liquid form, having excellent palatability, preventing coloring, and preventing the occurrence of diarrhea when administered enterally. Was difficult to make.

[0004] On the other hand, techniques for using L-arginine, glutamine or a glutamine-containing peptide as a nutritional composition include the following. (1) JP-A-2-191213 (2) JP-A-7-258075 (3) JP-A-6-9384 (4) JP-A-5-236909 (5) JP-A-7-255398 (6) International Publication WO95 / 11019 Specifically, the above-mentioned known technology is as follows.
Japanese Patent Application Laid-Open No. 2-191213 (1) discloses that "a) a compound related to the synthesis of a polyamine, b) a nucleobase source, c) an omega-3 polyunsaturated fatty acid and d) an immunization of an omega-6 polyunsaturated fatty acid. An immunostimulatory composition comprising a stimulatively effective amount of the aggregate is disclosed. JP-A-7-258 of (2)
No. 075 discloses "a highly storage-stable oral enteral nutrition composition characterized by containing an enzyme hydrolyzate of wheat gluten as a nitrogen source". JP-A-6 (3)
No. 9384 describes an orally ingestible nutritional composition having an improved taste and mouthfeel comprising a low PH amino acid wherein said amino acid is involved in the synthesis of arginine, valine and polyamines. Wherein said composition is selected from the group consisting of: JP-A-5-236909 of (4) describes that
"Peptide compositions containing 40% or more of glutamine or amino acids constituting glutamic acid" are disclosed. JP-A-7-255398 of (5) discloses that
An "oral enteral nutrition composition having high storage stability and palatability, characterized by containing an enzyme hydrolyzate of wheat gluten as a nitrogen source" is disclosed. International publication WO9 of (6)
No. 5/11019 discloses a digestive composition containing a sufficient amount of glutamine and describes its effect as a trigger for promoting the growth of hormones. As described above, oral administration of a nutritional composition containing L-arginine and / or glutamic acid or a glutamine-containing peptide has problems with palatability, particularly bitterness, and is described in JP-A-6-9384 of (3). The publication discloses a method for improving the composition by making bitterness acidic. Japanese Patent Application Laid-Open No. 7-255398 (5) discloses a method for improving bitterness by blending 40 to 80% of an enzyme hydrolyzate of wheat gluten as a glutamine-containing peptide. On the other hand, the combination of L-arginine and glutamine is a compound in a powder form, and has a problem that it requires time and effort to add and disperse it in water when used. Therefore, as described above, L-arginine and a glutamine-containing peptide are combined and contained in the form of a water-soluble emulsion, which is excellent in palatability and suppresses discoloration during heat sterilization. There is no known nutritional composition that is excellent in preventing the occurrence of germination.

[0005]

SUMMARY OF THE INVENTION A first object of the present invention is to provide an aqueous solution containing L-arginine and glutamine in combination to improve bitterness, and exhibit excellent color tone change upon heating and long-term stability. It is an object of the present invention to provide an emulsified nutritional composition. A second object of the present invention is to provide an oral and enteral nutritional supplement containing the nutritional composition as an active ingredient.

[0006]

Means for Solving the Problems The present inventors have conducted intensive studies in view of the above-mentioned conventional problems. As a result, the present inventors have found that by using L-arginine and a glutamine-containing peptide in combination, the pH of the solution is adjusted to a specific acidic region. The inventors have found that the above problems can be improved and completed the present invention. That is, the present invention includes the following (1) to (7). (1) It contains L-arginine as the component A and a glutamine-containing peptide as the component B, and has a pH of 5.5.
An aqueous emulsified nutritional composition that is -7.0. (2) Aqueous emulsified nutritional composition containing L-arginine as the A component, glutamine-containing peptide as the B component, and water-insoluble dietary fiber as the C component, and having a pH of 5.5 to 7.0 in the liquid. Stuff. (3) L-arginine as A component, glutamine-containing peptide as B component, water-insoluble dietary fiber as C component,
An aqueous emulsified nutritional composition containing a medium-chain fatty acid triglyceride as a D component and a nucleic acid as an E component, wherein the pH of the liquid is 5.5 to 7.0. (4) 0.5-1.5% by weight of L-arginine as the A component and 0.5-1.5% by weight of the glutamine-containing peptide as the B component.
An aqueous emulsified nutritional composition containing 2% by weight, comprising a nitrogen source, lipids, carbohydrates, vitamins, minerals, an emulsifier, and water, and having a pH of 5.5 to 7.0 with citric acid. (5) L-arginine of the component A is 0.5 to 1.5% by weight, and the glutamine-containing peptide of the component B is 0.5 to 2.0% by weight.
% By weight, 0.3-0.8% by weight of water-insoluble dietary fiber of component C, 0.1-0.1% by weight of medium-chain fatty acid triglyceride of component D
An aqueous emulsified nutritional composition containing 1.5% by weight and 0.3% by weight or less of a nucleic acid of the E component and having a pH of 5.5 to 7.0 in the liquid. (6) An oral nutritional composition containing the aqueous emulsion nutritional composition according to (1) to (5) as an active ingredient. (7) An enteral nutrient containing the aqueous emulsion nutrition composition as described in (1) to (5) as an active ingredient.

[0007]

BEST MODE FOR CARRYING OUT THE INVENTION The aqueous emulsion nutrition composition of the present invention comprises:
It is obtained by mixing L-arginine of the A component and the glutamine-containing peptide of the B component, and if necessary, other various nutrients, vitamins and minerals.

The L-arginine of the A component used in the aqueous emulsified nutritional composition of the present invention may be purified by decomposing gelatin, nucleoprotein of milt of fish, etc., but preferably produced and purified by fermentation. No.

The glutamine-containing peptide of the B component used in the aqueous emulsified nutrition composition of the present invention includes wheat gluten and a decomposition product of zein of corn protein. For example, as a commercially available product, a glutamine peptide WGE80GPA (trade name, which is a wheat gluten hydrolyzate manufactured by DMV) is preferably exemplified.

If necessary, the composition may further contain a water-insoluble dietary fiber as the C component, a medium-chain fatty acid triglyceride as the D component, and a nucleic acid as the E component.

The water-insoluble dietary fiber as the component C includes
For example, cellulose, hemicellulose, lignin, chitin and the like can be mentioned. Preferably, in order to improve the suspension stability of hydrolyzed crystalline cellulose from purified pulp, a water-soluble natural polysaccharide coated with, for example, karaya gum may be used. The average particle size is 14
It is preferably 0 μm or less. As a commercially available product, Avicel RC-N81 (trade name, manufactured by Asahi Kasei Corporation) may be mentioned.

Examples of the medium-chain triglyceride of the component D include triglycerides of medium-chain fatty acids having 8 to 10 carbon atoms. The fatty acids having 8 to 10 carbon atoms may be a single high-purity fatty acid or a mixture. Further, those fractionated from coconut oil, palm oil, palm kernel oil or the like, or those synthesized from medium chain fatty acids and glycerin may be used. Preferably, it is a fatty acid triglyceride having 8 to 10 carbon atoms. Specifically, as a commercial product, Panassate 81 manufactured by NOF Corporation
0 and the like.

As the nucleic acid of the E component, for example, as nucleosides, adenosine, guanosine, uridine, cytidine, deoxythymidine, deoxycytidine, and as nucleotides, adenylic acid, guanylic acid, uridylic acid, cytidylic acid, deoxythymidylic acid, deoxycytidylic acid Ziric acid and the like. Furthermore, RNA derived from yeast, nucleoprotein derived from milt of fish such as salmon, DNA, DNA sodium (manufactured by Nichiro Co., Ltd.) and the like are also included.

The mixing ratio of each component is as follows: component A, L-arginine, 0.5 to 1.5% by weight; component B, a glutamine-containing peptide, 0.5 to 2.0% by weight; Desirably, the dietary fiber is 0.3 to 0.8% by weight, the component D medium-chain fatty acid triglyceride, 0.1 to 1.5% by weight and the component E nucleic acid are 0.3% by weight or less. If the amount of L-arginine in the component A is less than 0.5% by weight, the effect of promoting the synthesis of polyamines and proteins is small. If the amount of the glutamine-containing peptide of the component B is less than 0.5% by weight, the effect as an energy source for intestinal mucosal cells is small. The water-insoluble dietary fiber of the C component is 0.
When the amount is less than 3% by weight, it becomes difficult to suppress the occurrence of diarrhea derived from L-arginine, and 0.8% by weight.
If the amount is larger, the viscosity of the product becomes high, and the fluidity of the product when it is sent through a tube or the like becomes poor. D
If the amount of medium-chain triglyceride in the component is less than 0.1% by weight, it is absorbed from the intestine and the effect as an energy source is small. Not preferred. If the amount of the nucleic acid of the component E is more than 0.3% by weight, it is unfavorable because it is excessively taken.

[0015] Further, as a nutritional composition, other ingredients are used in addition. As noted above, other formulations include nitrogen sources, lipids, carbohydrates, vitamins, and minerals. The amount added at that time is 0.25 ppm to 20 ppm.
% By weight.

Examples of the nitrogen source include amino acids, casein, casein sodium, milk protein, soybean protein, and enzymatically decomposed products of these proteins. The amount added at that time is 0.1 to 4% by weight.

Examples of the lipid include soybean oil, rapeseed oil, corn oil, perilla oil, fish oil and the like. Further, as the ω-3 or ω-6 polyunsaturated fatty acid, for example, eicosapentaenoic acid and α-linolenic acid are exemplified as ω-3 polyunsaturated fatty acid, and fish oil and perilla oil are contained as fats and oils containing a large amount thereof. No. Linoleic acid and arachidonic acid are listed as highly unsaturated fatty acids of ω-6, and soybean oil and corn oil are listed as fats and oils containing a large amount thereof. Fats and oils having high contents of these components may be used as they are, or purified high-purity products may be used. The amount added at that time is 0.1 to 3% by weight.

Examples of the saccharide include dextrin, oligosaccharide, sucrose and the like. In order to suppress coloring of the product, dextrin is preferred, and those having a low degree of decomposition are more preferred. For example, the degree of degradation is expressed as DE, and includes dextrins with DE = 8 to 15. As commercially available products, trade name par index # 2, (DE = 1
1) Matsuya Chemical Co., Ltd. is preferred. The amount added at that time is 5 to 20% by weight.

As vitamins, for example, vitamin A,
Vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, vitamin D, vitamin E, vitamin K, nicotinic acid, folic acid, thiamine, riboflavin, niacin, biotin, pantothenic acid, β-carotene and the like. These vitamins may be used by blending them in advance based on the required nutritional amount. The amount added at this time is 0.01 to 0.1% by weight.

As minerals, for example, sodium,
Potassium, calcium, magnesium, manganese, zinc, copper, iron, selenium, chromium, molybdenum, phosphorus, chlorine and the like. These minerals may be used by blending a compound containing them in advance based on nutritional requirements. The amount added at that time is 0.4 to 1% by weight.

As the emulsifier used in the present invention, for example,
Poly or monoglycerin fatty acid ester, sorbitan fatty acid ester, propylene glycol fatty acid ester, sucrose fatty acid ester, organic acid monoglyceride, lecithin and the like can be mentioned. Preferably, among them, succinic acid monoglyceride, glycerin monofatty acid ester, sorbitan monofatty acid ester and the like are included. More preferred is succinic monoglyceride. The amount added at that time is 0.1 to 1% by weight.

For lowering the pH of the solution, for example, phosphoric acid, acetic acid, malic acid, citric acid, lactic acid, hydrochloric acid, amino acids and the like can be used. In particular, food grade ones are desirable from the point of application. The pH of the liquid must be 5.5 to 7.0. If the pH of the solution is lower than 5.5, protein aggregation is likely to occur, and it is difficult to obtain a stable emulsion, and if the pH of the solution is higher than 7.0, the flavor of the nutritional composition deteriorates as described later.

Further, from the viewpoint of improvement of palatability, for example, flavors, flavors for flavor, flavors and herb essences, and coloring agents and antioxidants may be added. .

In order to prepare the above-mentioned nutritional composition, for example, the above-mentioned components are added in a lump, or the ingredients are added in order, and a known method such as a stirrer, a circulator, a high-pressure homogenizer, a disperser, It can be prepared using a mechanically powerful dispersing machine such as a microfluidizer. The temperature during compounding is not particularly limited, but is preferably 10 to 80C, more preferably 40 to 60C. The stirring condition is not particularly limited, but is 10 minutes to 24 hours. More preferably, it is 20 minutes to 1 hour.

Further, if necessary, it is preferable to perform a treatment such as heat sterilization, for example, for long-term storage.
As the treatment such as heat sterilization, specifically, 100 to 15
It is 3 seconds to 20 minutes at 0 ° C.
0 to 140 ° C., 20 seconds, 110 to 1 in retort sterilization
30 ° C. and 5 to 15 minutes are desirable.

The aqueous emulsified nutritional composition of the present invention can be orally administered to a sick patient as it is, and does not need to be dispersed or dissolved at the time of use like a powder. Also, as an enteral nutrition composition, via a tube before and after surgery,
It is also possible to administer. It depends on the condition before and after the operation, the patient's weight and appetite, etc.
0 ml / kg body weight / day, preferably 20-50
ml / kg body weight / day.

[0027]

The aqueous emulsified nutritional composition of the present invention contains L-arginine and a glutamine-containing peptide.
Since H is in a specific region of acidity, the coloring of the product at the time of blending and the improvement against bitterness at the time of ingestion are obtained. It is also possible to prevent coloring of the product and improve long-term stability. Furthermore, since the aqueous emulsion nutrition composition of the present invention contains various components, it is effective for immunological activation and activation of protein metabolic system to prevent infectious diseases and to heal wounds at an early stage.

[0028]

The present invention will be described in more detail with reference to the following examples. Reference Examples 1 to 7 L-arginine, a glutamine-containing peptide, sucrose and citric acid were blended as shown in Table 1, and the relationship between the pH and the taste of the solution was examined. The results are shown in Table 1.

[0029]

[Table 1]

From the results, the pH of the solution was adjusted to 5.5 to 7.0.
It turned out that bitterness could be improved. Example 1 A stainless steel container equipped with a stirrer was charged with 78.00 kg of purified water at about 60 ° C, and L-arginine, 0.7
5 kg and then 0.13 kg of citric acid were added and dissolved. Furthermore, 0.60 kg of a mineral mix (containing the following components previously blended) and a glutamine-containing peptide (DMV, trade name glutamine peptide WGE80G)
PA) 1.50 kg, sodium caseinate (manufactured by Chuo Shoko Co., Ltd., trade name: Instantrac S) 2.0 kg, and dextrin (manufactured by Matsutani Chemical Co., Ltd.), trade name: Paindex # 2, decomposition degree DE = 11｝ 14 kg And 0.02 kg of a vitamin mix were sequentially added and dissolved. Next, a succinic acid monoglyceride preparation as an emulsifier (manufactured by Riken Vitamin Co., Ltd., trade name: Panmac 200 (B)) 0.3
kg, 2.7 kg of soybean oil and a further small amount of a flavor (milk flavor) were added, and the mixture was preliminarily emulsified at 60 ° C. for 20 minutes. Thereafter, using a high-pressure homogenizer (manufactured by Sanwa Machine Co., Ltd.), the pressure was homogenized by applying a homogenizing pressure of 500 kg / cm ² , and the mixture was packed in an aluminum pouch. Thereafter, the temperature was 122 ° C. and the time was 6 minutes. Retort sterilization was performed under the conditions. Table 2 shows the measurement results of the prepared aqueous emulsion nutrition composition.

The mineral mix used was previously blended with a commercially available salt containing each component, and the components were as follows. Sodium 153.33 mg / g potassium 195.00 mg / g calcium 66.67 mg / g phosphorus 96.67 mg / g magnesium 33.33 mg / g iron 1.67 mg / g

In addition, the vitamin mix used contained the following components in 1 g in advance. Vitamin A 10,000 IU Vitamin B1 5 mg Vitamin B2 7 mg Vitamin B6 12 mg Vitamin B12 12 μg Vitamin C 360 mg Vitamin D 625 IU Vitamin E 50 mg Nicotinic acid 85 mg Folic acid 1 mg Pantothenic acid 50 mg

[0033]

[Table 2]

(Note) The emulsifier is a succinic monoglyceride preparation (manufactured by Riken Vitamin Co., Ltd., trade name: Panmac 20).
0 (B)} was used.

The respective measuring methods are shown below. 1. PH: Food Additives Standard, P of General Test Method 29.0
It was measured according to the H measurement method. 2. Viscosity: Measured using a B-type viscometer (Tokyo Keiki, BL adapter). 3. Osmotic pressure: Measured by the freezing point depression method using an advanced osmometer 3W2 type (manufactured by Advance Corporation). 4. Color tone: Converted to whiteness (HW) based on JIS Z 8729 (1980). Model used: A color difference meter, Car Ace TC-8600 type (manufactured by Tokyo Electric Co., Ltd.) was used. 5. Flavor; about 5 g sample of nutritional composition by 5 panelists
Was evaluated in the following three stages. Evaluation point Evaluation 1; Poor flavor 2; normal. 3; Drink without bite. Good flavor. The total score was determined and indicated by the following symbols. Evaluation symbol; Total score ○: 12 to 15 Δ; 8 to 11 ×; 5 to 7 6. Emulsified state: 100 ml of the prepared emulsified liquid was placed in a 100 ml heat-resistant wide-mouth bottle, sealed, sterilized at 122 ° C. for 6 minutes, left at 30 ° C. for 1 week, and visually observed for the following conditions. It was evaluated on a scale. Evaluation symbol ○: no change △: creaming occurred ×: separated into two layers

7. Convenience test (enteral) A test was conducted using the samples of the aqueous emulsified compositions of Examples and Comparative Examples by the following animal test. 1) Experimental animals and breeding method: A 5-week-old SD male rat was preliminarily bred for 1 week on a commercially available solid feed (CE-2, manufactured by CLEA Japan) and used for the test. Rat breeding conditions are as follows. (A) Temperature 22 ± 2 ° C (b) Humidity 50 ± 10% (c) Artificial lighting 12 hours a day (from 6:00 am to 6:00 pm) (d) Rearing individually in SPF animal facilities 2) Implementation Administration of the aqueous emulsified nutritional compositions of Examples 1 to 5 and Comparative Examples 1 to 4; about 19, using an infusion pump via a catheter placed in the duodenum of a rat.
An amount of 230 ml / kg body weight was administered over time. The administration period was 4, 5, or 7 consecutive days. 3) Examination of stool The stool was observed every day during the administration period and evaluated in the following four stages. ×: watery stool (feces that do not maintain the shape of feces) △: loose stools (feces that maintain the shape of feces but adhere to the funnel in the cage) ○; normal stools (feces that maintain the shape of feces and No stool-)

8. Stool test (oral) Experimental animals and breeding methods were the same as in the above-mentioned stool test (enteral). The administration of the aqueous emulsion composition was 5.5 m
1 / time was administered by gavage eight times a day (every hour from 9 am) using a gastric tube. The administration period was for four consecutive days. The stool test was evaluated in the same manner as the stool test (enteral).

9. Lymphocyte blastogenesis test 1) Experimental animals and breeding method; 4-week-old SD male rats were pre-fed for 1 week on a commercial solid feed (CE-2, manufactured by CLEA Japan, Inc.). Using. The breeding conditions of the rats are as follows. (B) Temperature 22 ± 2 ° C (b) Humidity 50 ± 10% (c) Artificial lighting 12 hours a day (from 6:00 am to 6:00 pm) (d) Rearing individually in SPF animal facilities (e) The feed is a solid feed (C
E-2, manufactured by Nippon Clea Co., Ltd.) and drinking water (water suitable for the water quality standard of the Tap Water Law) were freely given. 2) Administration of test substance: 80 ml / kg body weight / day (20 m
1 / kg body weight × 4 times / day) was administered orally by gavage using a gastric tube. The administration period was for four consecutive days. In addition, as a control group, a commercially available enteral nutritional supplement (trade name: ENSURE LIQUID: manufactured by Dynabot) to which L-arginine and glutamine-containing peptide were not added was similarly administered. The number of animals used was 8 males and 8 females. 3) Measurement of immaturity; fasting for 24 hours after the end of the administration period,
Pentobarbital sodium (Tokyo Chemical Industry Co., Ltd.)
A 6.48 mg / ml aqueous solution was intraperitoneally administered at 5 ml / kg body weight, blood was collected from the abdominal aorta under anesthesia, and treated with a lymphocyte preservation solution. The blastogenesis reaction with concanavalin A (abbreviated as ConA) was measured by ³ H-thymidine uptake assay. 4) Evaluation: Evaluation was performed as follows. ；: In the average value, the test group is higher than the control group. Strong juvenile response. X: The test group is equal to or less than the control group in the average value.
Juvenile response is equivalent or weak.

10. Atrophy test of intestinal mucosa 1) Microscopic observation: In the above test 9, blood for measurement was collected, exsanguinated, fixed intestine intestine with 10% neutral buffered formalin, and sliced in accordance with a conventional method. And H. was prepared. E. FIG. After staining, the cells were observed under a microscope. 2) Evaluation; Evaluation was made as follows in comparison with the test group. ；: There is no tissue abnormality, and the villus height is kept equal to or higher than that of the control. ×: Tissue abnormality is observed, or villous atrophy is observed as compared with the control.

Examples 2 to 4 Except that each component was changed to have the composition shown in Table 2,
An aqueous emulsion nutrition composition was prepared in the same manner as in Example 1.
Each measurement was performed similarly. The results are shown in Table 2. As the water-insoluble dietary fiber as the C component, crystalline cellulose (trade name: Avicel RC-N81, manufactured by Asahi Kasei Kogyo Co., Ltd.) is used, and as the medium-chain fatty acid triglyceride as the D component, panassate 810 {Nippon Yushi ( Co., Ltd.
As a nucleic acid as an E component, DNA sodium ｛
A predetermined amount of Nichiro's trade name 用 い was used.

Example 5 A stainless steel vessel equipped with a stirrer was charged with 78.48 kg of purified water at about 60 ° C., and L-arginine, 0.7
5 kg, Avicel RC-N8 as water-insoluble dietary fiber
(1) 0.5 kg (trade name, manufactured by Asahi Kasei Corporation) and 0.13 kg of citric acid were added and dissolved. Furthermore, mineral mix (containing the above-mentioned components previously blended)
0.60 kg, glutamine-containing peptide (DMV, brand name glutamine peptide WGE80GPA) 1.6 k
g, DNA sodium ｛Nichiro Co., Ltd.｝ 0.12k
g, casein sodium
Chuo Shoko Co., Ltd. 2.0 kg and dextrin manufactured by Matsutani Chemical Co., Ltd., trade name Paindex # 2, decomposition degree DE = 11-11 kg, sucrose 2.0 kg, vitamin mix 0.02 kg In addition, it dissolved. Next, succinic acid monoglyceride as a emulsifier (trade name: Panmac 200 (B) manufactured by Riken Vitamin Co., Ltd.): 0.3 k
g, corn white squeezed oil 1.3 kg, and medium-chain fatty acid triglyceride Panassate 810, 1.2 k, manufactured by NOF Corporation
g, and a mixture of a small amount of a flavor (milk flavor) was further added, followed by preliminary emulsification at 60 ° C for 20 minutes. Thereafter, using a high-pressure homogenizer (manufactured by Sanwa Machine Co., Ltd.), the pressure was homogenized by applying a homogenizing pressure of 500 kg / cm ² , and the mixture was packed in an aluminum pouch.
The retort sterilization was performed at a temperature of 6 ° C. for 6 minutes.

Comparative Examples 1 to 5 Aqueous emulsified nutritional compositions were prepared in the same manner as in Example 1 with the composition shown in Table 3. Table 3 shows the results of similar tests.
Are also shown. Comparative Example 3 used water-soluble dietary fiber, and Comparative Example 4 used water-insoluble dietary fiber.

[0043]

[Table 3] (Note) As the water-soluble dietary fiber, corn fiber (Nisshoku Cell Ace, manufactured by Nippon Shokuhin Kako Co., Ltd.) was used.

From the above results, the system containing only L-arginine or glutamine-containing peptide alone does not satisfy all of the color tone, flavor, emulsification state and stool test results, and undesirably causes one or more problems. . It was confirmed that, even with a combination of L-arginine and a glutamine-containing peptide, if the pH exceeded 7.0, poor coloring and poor flavor occurred, which was not preferable. On the other hand, when L-arginine and a glutamine-containing peptide were combined and the pH was maintained at 5.5 to 7.0, it was found that the color tone, flavor, emulsified state, and results of the stool test were all good. .

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁶ Identification code FI A61K 31/70 A61K 31/70 31/715 31/715 33/00 33/00

Claims (7)

[Claims] An aqueous emulsified nutritional composition comprising L-arginine as the component A and a glutamine-containing peptide as the component B, wherein the pH of the solution is 5.5 to 7.0. 2. An aqueous emulsion containing L-arginine as component A, glutamine-containing peptide as component B, and water-insoluble dietary fiber as component C, and having a pH of 5.5 to 7.0 in the liquid. Nutrition composition. 3. It contains L-arginine as the A component, glutamine-containing peptide as the B component, water-insoluble dietary fiber as the C component, medium-chain fatty acid triglyceride as the D component, and nucleic acid as the E component, and PH of the liquid is 5.5 to 7.0
An aqueous emulsion nutrition composition. 4. The method according to claim 1, wherein the component A L-arginine is added in an amount of 0.5 to 1.5
Glutamine-containing peptide of 0.5% by weight and B component
2% by weight, and further contains a nitrogen source, lipids, carbohydrates, vitamins, minerals, emulsifiers, and water.
An aqueous emulsified nutritional composition comprising H of 5.5 to 7.0. 5. The composition according to claim 1, wherein L-arginine of component A is 0.5 to 1.5.
0.5% to 2.0% by weight of the glutamine-containing peptide of the B component, 0.3 to 0.8% by weight of the water-insoluble dietary fiber of the C component, and 0.1% of the medium chain fatty acid triglyceride of the D component. An aqueous emulsified nutritional composition containing 1.5 to 1.5% by weight and 0.3% by weight or less of the nucleic acid of the E component, and having a pH of 5.5 to 7.0 in the liquid. 6. An oral nutritional supplement comprising the aqueous emulsified nutritional composition according to claim 1 as an active ingredient. 7. An enteral nutritional composition comprising the aqueous emulsion nutrition composition according to any one of claims 1 to 5 as an active ingredient.