JPH10276766A - How to use beer yeast - Google Patents

Abstract

(57) [Summary] To use yeast derived from the beer production process, the outer wall and the cell membrane must be removed. The invention relates to the method. According to the present invention, the microorganisms can be selectively cultured, and the cultured microorganisms can be used for selective wastewater treatment, for example, only oil can be removed from wastewater containing miscellaneous components. The present invention illustrates that method. SOLUTION: When glucose is added to brewer's yeast, the outer cell membrane can be removed. The brewer's yeast that has been removed and solubilized contains phenylalanine, which is consumed only by microorganisms that secrete lipase enzymes. Therefore, a microorganism capable of selectively secreting a lipase enzyme can be cultured. Lipase enzymes degrade oils and fats in wastewater well. Therefore, treatment of oil-containing wastewater is possible. In this case, the microorganism is activated when the solubilized beer yeast is provided.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

[0001] The present invention relates to a method for processing and recycling yeast derived from beer production, and a method for processing waste yeast produced in beer production according to the present invention. Alternatively, the microorganism can be selectively cultured. The method, and thereby, the industrial wastewater can be selectively treated. Regarding the method.

[0002]

2. Description of the Related Art Brewer's yeast derived in the course of production contains various vitamins, amino acids and various minerals. However, these nutrients cannot be used as they are because they are covered by the outer wall and cell membrane.
Conventionally, waste yeast that accumulates in the tank bottom in the beer production process is collected,
I've been dry and crushed to feed fish and livestock,
The excretion rate is high because the outer wall and cell membrane are obstructed and are not sufficiently digested. Even if you try to make up for the problem by giving it a large amount, it is difficult to handle it with the outer wall and cell membrane because it cannot be dissolved in water. Even if the crushing after drying is strengthened to break the outer wall and cell membrane, the crushing is only slight enough to break the nutrients contained therein. There is a method of extracting by heating, pressurizing, etc. using various solvents, but it is expensive and not very practical.

[0003]

It is an object of the present invention to utilize nutrients in the brewer's yeast except for the outer wall and cell membrane of the brewer's yeast. Currently, urban sewage treatment plants
Disturbed by the fats and oils contained in the incoming sewage, the original B
The function of removing OD is reduced. It is forbidden by law to run oils and fats into rivers, but wastewater from food production and restaurant kitchens contains significant amounts of oils and fats, and struggles to comply with the law. The present invention aims to remove fats and oils contained in wastewater using waste yeast derived from a beer production process. To remove oils and fats in wastewater,
There is a need for a microorganism that secretes lipase, which is a fat-degrading enzyme. It is also an object of the present invention to preferentially culture such microorganisms by controlling other coexisting microorganisms.

[0004]

The means starts with removing the outer wall and cell membrane of brewer's yeast using glucose and dissolving it in water. Next, a microorganism that secretes lipase (lipolytic enzyme) is cultured using it. Then, the oil-containing wastewater is treated using the cultured lipase-secreting microorganism. In the beer production process, the yeast settles to the bottom of the tank over time during the main fermentation and subsequent ripening. This is recovered and used in the present invention. Both yeasts generated during the main fermentation period and yeasts generated during the ripening period can be uniformly used in the practice of the present invention. The recovered yeast can be used as it is, or it can be dried and pulverized to a flour-like powder. 167 g of hydrous crystalline glucose is weighed against 500 g of the recovered yeast in terms of dry matter, and mixed in a dry state. Preliminarily fill a container with water at a depth of about 5 cm, and spray the mixed yeast and glucose from above so that the water surface is covered. In this case, never stir. When mixed with water, it becomes sticky, step-like dumplings, and the reaction of glucose to yeast becomes uneven. When the spraying is finished so that the surface of the water is covered, sprinkle water on it and moisten it. Sprinkle water and cover the mixture of yeast and glucose in the water.
Make a layer of water. Again, do not stir. Slightly press down the mass of the mixture to be lifted with a spatula. If you see that no mass of the mixture floats on the surface of the water and there is nothing to expose, leave the lid on for 1-3 days and the water will turn red. In this case, if glucose is 2 or less (dry weight ratio) with respect to the brewer's yeast 10, it does not become red even after 3 days. That is, the outer wall and cell membrane of yeast are not removed by glucose. On the other hand, if the amount of water used is smaller than the specified amount, the whole will collect in a muddy state and the purpose of removing the outer wall cannot be fulfilled. Even if the amount of yeast used is more than the specified amount of glucose, the yeast becomes muddy and cannot achieve the purpose of removing the outer wall. The water-added mixture of beer yeast and glucose thus obtained is gently stirred with a spatula to make the mixture up and down uniformly. When the mixture was homogenized and poured into 54 l of water, the whole became yellow-brown. After the addition was completed, the mixture was completely dissolved without any insoluble matter remaining at the bottom even when left to stand overnight. The nutrients contained in 54 l are as shown in Table-1.

[0005]

[Table 1]

[0006] Thus, the outer wall and the cell membrane can be easily removed by using glucose, and various nutrients contained in the brewer's yeast can be used in a solution state. Particularly valuable as nutrients are vitamins such as vitamins B, pantothenic acid, inositol, lysine,
Amino acid compositions such as phenylalanine, leucine, glutamic acid, aspartic acid, tryptophan, and nucleic acids. All of these nutrients are preferred nutrients for culturing microorganisms, and among them, phenylalanine has a characteristic that only microorganisms secreting lipolytic enzymes prefer to eat and other microorganisms are not involved.
Even if about 2 mg / l of phenylalanine is present in the culture water, lipolytic enzyme (lipase) secreting microorganisms increase.
Since brewer's yeast contains about 2.2% of phenylalanine, it is easy to maintain the concentration of phenylalanine in the culture water at 2 mg / l or more by using the brewer's yeast solution in the method according to the present invention. Alternatively, microorganisms that degrade oil and fat can be cultivated. A method for treating oil-containing wastewater using the microorganisms thus cultured will be described below.

[0007] 334 g of hydrous crystalline glucose (Showa Sangyo Co., Ltd.) is mixed with 1000 g of the recovered yeast dried to a water content of 6.5%, combined with water, dissolved for 3 days, dissolved in 54 l of water, and aerated. The solution stirred overnight was a soy oily tan liquid with a BOD value of 12400 mg / l. Biochemical treatment of wastewater using the solution obtained according to the present invention is performed using the value of BOD.
When a solution having a BOD of 12400 mg / l is added and the BOD of the wastewater after the addition is set to 500 mg / l or more,
The oil and fat content in the wastewater initially contained at about 300 mg / l is reduced to about 60 mg / l in 6 hours. In this case, when the water temperature is maintained at 20 to 30 ° C., the pH is adjusted to 6.8 to 7.4, and the amount of dissolved oxygen is adjusted to 2 to 4 mg / l by adjusting the aeration, the lipolytic enzyme is taken in 16 hours. Microorganisms that secrete 50
Proliferate more than twice and increase the reaction rate, initially 300 mg / l
The fat content of the raw water is reduced to 30 mg / l or less.

[0008]

DESCRIPTION OF THE PREFERRED EMBODIMENTS The embodiments of the invention are constituted by the following three embodiments. First Embodiment An operation of removing the outer wall and cell membrane of yeast collected from a beer production process using glucose. Second form The yeast from which the outer wall and cell membrane have been removed is dissolved in water, and BOD 5
An operation in which a solution of 00 mg / l or more is prepared, and a microorganism selected for each purpose, for example, a microorganism that secretes an enzyme that decomposes fats and oils in wastewater, is added to the solution, and the culture is expanded. Third Embodiment An operation of treating oleaginous wastewater by using cultivated microorganisms and using solubilized brewer's yeast together to promote activation in the reaction. The above three forms are related and not isolated. Embodiments of the present invention will be described in detail with reference to Examples, but the present invention is not limited thereto.

[0009]

Example 1 During the maturation period of the beer production process, the live yeast collected from the bottom of the tank, which was generated with time, was taken out and dried (product name: dried beer yeast B product) in a powder form having a water content of 7.4%. (45 mesh pass), it was egg-shaped when viewed under a microscope, the outside was surrounded by a cell wall, the inside of the cell wall contained cytoplasm wrapped in a cell membrane, and the cytoplasm contained various nutrient components. However, as it is, it is difficult to dissolve in water, and even when added to water, it suspends and sediments. However, 167 g of hydrous crystalline glucose (Showa Sangyo product) is thoroughly mixed with 500 g of dried beer yeast B product in a dry powder state, and then sprayed from above onto 2 l of water to cover the surface of the water, and the remainder is sprinkled on the spatula. Lightly leveled. Water was added in a mist from above to uniformly wet the whole. Then, water is evenly sprayed on the moistened powder surface, the powder surface is covered with water,
An aqueous layer was formed at a height of 3 cm. The container used is a 10 l poly horseshoe with a lid. When the lid was covered and allowed to stand for 24 hours, the water layer on the surface was colored red. After transferring to a 50 liter plastic container and adding 30 liters of water and performing aeration and stirring for about 6 hours, a pale yellowish white, slightly brownish solution was obtained. Even after one night, almost no muddy deposit was observed at the bottom of the tank, and the added brewer's yeast was completely dissolved.

(Comparative Example 1) When glucose is not blended with brewer's yeast, the same method as in Example 1 is used, but after one night, almost all of the added brewer's yeast is deposited on the bottom. . Table 2 shows the differences between the examples and the comparative examples in terms of the dissolution rate of the crude protein contained in the brewer's yeast. The effect of glucose is clearly understood from this table.

[0011]

[Table 2]

[0012]

Example 2 The raw yeast collected at the bottom of the tank during the main fermentation period of the beer production process was taken out and dried (trade name: dried beer yeast A product) in a powder form with a water content of 7.5% (45 mesh pass). As in the case of the product B used in Example 1, it was egg-shaped, the outside was surrounded by the cell wall, and the inside of the cell wall was wrapped in the cell membrane and contained various nutrients. This one (dried beer yeast A
334 g of the hydrous crystalline glucose used in Example 1 was added to 1000 g of the product, and the outer wall and cell membrane were removed in the same manner as in Example 1, and then added to 360 L of catering center wastewater. Then, 30 g of Bacillus subtilis (trade name: BNClean BN1001) was inoculated as an original seed, and aeration culture was performed at 24 ° C. for 72 hours.
9% melted, so that the BOD of the culture broth is 2850 mg
/ L, and the nutrients in the culture solution were as shown in Table 3. As a result, the number of BN bacteria, which was 2 × 10 ⁶ cells / g at the beginning of the culture, was increased to 3 × 10 ⁸ cells / g by 100 times or more.

[0013]

[Table 3]

[0014] The growth of the bacteria was also observed depending on the pH of the culture solution. That is, at the beginning of the culture, the pH of the culture solution is 6.
It was 8. It increased with the progress of the culture, and reached 7.6 on the third day of the culture, whereby the bacterial concentration increased 100-fold. And on the 5th day of culture, it goes up to 8.2,
The bacterium was too self-digesting.

(Comparative Example 2) When the operation of removing the outer wall and cell membrane using glucose was not performed, the same operation as in Example 2 was carried out, and Bacillus subtilis (trade name: BNCLEAN) was added to a 360-liter catering center wastewater. BN1001),
Inoculated with 30 g as the original seed, with a bacterial concentration of 2 × 10 ⁶ cells / g
Then, aeration culture was performed at 24 ° C. for 72 hours, but the initial pH value of 6.8 was kept at the culture solution 6.9 for 72 hours.
The initial pH is the same, and the bacterial concentration is proportional to 2.6 ×
At 10 ⁶ cells / g, there was no increase from the initial bacterial concentration. Even after culturing for 120 hours until the 5th day,
The pH did not rise and the bacteria did not grow by the time autolysis occurred.

[0016]

Example 3 In Example 2, a reaction vessel having a capacity of 3000 l
300 l of culture solution with a bacterial concentration of 3 × 10 ⁸ cells / g cultured for a day
And add 2700 l of wastewater from a catering center containing 320 mg / l of oil in normal hexane extraction value,
Aeration was performed while circulating through the reaction vessel at a flow rate of 1 / m for 12 hours. After that, at a flow rate of 10 l / m, a normal hexane extraction value of 320 mg / l oil-containing wastewater from a catering center was continuously introduced at a reaction tank residence time of 5 hours, and a normal hexene extraction value of the effluent of the reaction tank outlet was measured. Then, 21mg / l,
That is, the oil content of the wastewater, which was initially 320 mg / l,
It was reduced to 21 mg / l. The oil removal rate was 93%. The hexane extraction value of the influent water is 120-380 mg /
but the flow rate was 10 l / m, and 3
The hexane extraction value of the effluent was always 30 mg / l or less, even if the water was passed continuously for days. After three days,
The flow of raw water was stopped, 300 l of the culture obtained in Example 2 was added again, and the mixture was circulated in the reaction tank at a flow rate of 20 l / m for 12 hours. Prior to circulation, 300 l of a brewer's yeast solution containing no 30 g of the original seed prepared by the method of Example 2 was added in order to activate bacteria remaining in the reaction tank. After performing a batch operation of circulation in the tank for 12 hours, the operation was switched to a continuous water flow operation, and water was passed at 10 l / m. 3 water flow
Continued for days. During that time, the oil content of the influent (normal hexane extraction value) fluctuated from 90 mg / l to 420 mg / l, but the normal hexane extraction value of the effluent water was always 30 mg / l.
At l or less, the oil removal rate could be stably maintained at 87% or more.

[0017]

EFFECT OF THE INVENTION Beer yeast contains a large amount of protein and magnesium which are required by microorganisms in terms of growth structure (body tissue). It also includes growth factors (gross factors). Vitamins (especially group B), amino acids (especially essential amino acids)
And the like are mentioned as growth factors of microorganisms. Conventionally, the removal of those substances was difficult, costly, complicated, and required equipment, but in the present invention, it is easy and troublesome, and equipment such as heating and power is not required. Can be accomplished. Precious and trace amount of vitamin B
_12. Biotin can be extracted without compromising quality. Conventionally, brewer's yeast derived from production is large in amount and sometimes difficult to dispose of, but the present invention can be processed in large amounts without incurring costs.
The soluble yeast obtained according to the present invention can be used for a variety of purposes, but the effect of growth and activation in culturing microorganisms is particularly large. The cultivation can be easily controlled by the BOD value and the pH value, and it is possible to grow, for example, Bacillus secreting lipolytic enzymes 100 times in three days. Increase the concentration of bacteria in the reaction tank for wastewater treatment using microorganisms,
Because it can be activated, the reaction tank can be small,
The required area can also be saved. Further, the generation of sludge due to the wastewater treatment is small, and the microorganisms can be fixed in the reaction tank, and the wastewater can be treated with non-sludge.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁶ Identification symbol FI // (C12N 1/20 C12R 1: 125) (C12N 9/20 C12R 1: 125)

Claims (2)

[Claims] 1. A method in which a yeast recovered in a beer production process is treated with glucose to remove the outer wall and cell membrane of the yeast, and to utilize various components contained in the yeast. 2. A method for culturing microorganisms using the yeast collected in the beer production process and treating wastewater using the cultured microorganisms.