JPH1046142A - Antioxidant - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an antioxidant which has a very potent antioxidant effect, is nonproblematic in toxicity, and has possible uses in many fields including foods cosmetics, etc., by using an extract of Saxifraga stolonifera of the saxifrage family as the active ingredient. SOLUTION: This antioxidant contains as the active ingredient an extract of Saxifraga stolonifera, obtained by subjecting a mixture of the whole body (including leaves, stems, fruits, etc.) of this plant with an extractant such as water or ethanol to cold immersion or heating under reflux, filtering the product, and concentrating the filtrate. It is suitable that the antioxidant contains 0.001-20.0wt.% (in terms of a dried product), based on the entire antioxidant, above extract. As compared with conventional antioxidants, it can exhibit a marked antioxidant effect and a potent antioxidant effect as compared with an antioxidant obtained from Hydrangea hortensis Sm. var. oamacha Makino of the same family. It can be stably added to a system containing a substance which has a poor oxidation stability and is difficultly stabilized by the addition and has possible uses in many fields including cosmetics, foods, etc.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a novel antioxidant having an excellent antioxidant action.

[0002]

BACKGROUND OF THE INVENTION Hitherto, dibutylhydroxytoluene (BHT), butylhydroxyanisole (BHA), tocopherol and the like have been used as antioxidants for foods and cosmetics containing oils and fats. However, synthetic antioxidants such as BHT and BHA have safety problems, and the amount added is limited. Therefore, natural antioxidants have been desired. The present invention has been made in view of such conventional circumstances, has a high effect as an antioxidant, has no toxicity problem, and can be used as food, cosmetics, pharmaceutical raw materials and the like. It is intended to provide an antioxidant having a high content.

[0003]

Means for Solving the Problems In order to achieve the above object, the present inventors have intensively studied the antioxidant activity of natural products, and as a result, it was found that Saxifraga stolonifer (scientific name: Saxifraga stolonifer) of the family Saxifragaceae.
a) The plant extract was found to be extremely effective as an antioxidant.

[0004] That is, the present invention is an antioxidant characterized by containing a plant extract of Saxifraga stolonifera of the family Saxifragaceae as an active ingredient.

Hereinafter, the configuration of the present invention will be described in detail. Saxifragaceae of the family Saxifragaceae used in the present invention (scientific name: Sa
All of the xifraga stolonifera) plant extracts have been known to have a testosterone-5α-reductase inhibitory effect, a whitening effect, and an anti-inflammatory effect (Japanese Patent Application Laid-open No. Hei 5 (1993) -107).
-17365, JP-A-5-58870, JP-A-5-139951), and cosmetics. However, it is not yet known that these components have an antioxidant action, and they have been found for the first time by the present inventors.

[0006] Saxifraga (scientific name: Sa) used in the present invention
The xifraga stolonifera) plant is a perennial plant that is distributed abundantly on humid rocks especially in the mountains of Japan (excluding Hokkaido) and China. The extract used in the present invention is obtained by subjecting the whole plant, such as the leaves and stems or fruits of the above plants, to saxifrage seeds to cold soaking or heating to reflux with an extraction solvent, followed by filtration and concentration.
The extraction solvent used in the present invention may be any solvent as long as it is a solvent usually used for extraction, particularly water, or alcohols such as 1,3-butanediol, ethanol and methanol, hydrous alcohols, acetone and ethyl acetate. Organic solvents can be used alone or in combination.

The amount of the Saxifraga extract in the present invention is 0.001 to 20.0% as a dry matter in the total amount of the external preparation.
% By weight is appropriate. If it is less than 0.001% by weight, the antioxidant power is insufficient, and if it exceeds 20.0% by weight, it is unnecessary in terms of antioxidant power. It is not practical because it gets worse.

[0008] Cosmetics are preferred as the object to be blended as an antioxidant, and when added to various cosmetics requiring an antioxidant effect, for example, creams, lotions, emulsions, hair oils, shampoos, rinses, soaps, etc., have excellent antioxidant power. Demonstrate. Other additives to be added to such cosmetics include, for example, oils, surfactants, thickeners, neutralizers, preservatives, powder components, pigments, sequestering agents, fragrances, and ultraviolet absorption. Agents, medicinal agents, etc., and are used in appropriate combinations as needed. As a matter of course, the antioxidant of the present invention can be used in combination with other antioxidants such as vitamin E, licorice extract, BHT, and BHA as long as the effects of the present invention are not impaired.

In particular, the use of the antioxidant of the present invention makes it possible to stably mix even a substance which has conventionally been considered difficult to mix due to poor oxidation stability. For example, turtle oil, mink oil, and the like, which are expected to have an emollient effect when applied to cosmetics, have poor oxidative stability, and their use is limited. By adding, the fats and oils can be prevented from being oxidized, and these fats and oils can be effectively incorporated into cosmetics. Recently, application of an antioxidant has been proposed for the purpose of preventing lipid peroxidation reaction in a biological system, but the antioxidant of the present invention can of course be used in a biological system.

[0010]

[Action] Saxifraga of the family Saxifragaceae (scientific name: Saxifraga
stolonifera) The strong antioxidant activity of the plant extract was confirmed by the following experiments by the present inventors.

(1) Hydroxy Radical Scavenging Activity Test A mixture of 50 μl of a 1 mM ferrous sulfate (FeSO ₄ ) solution and 75 μl of a sample (aqueous solution) shown in Table 1 was mixed with 5,5-dimethyl-1-pyrroline oxide (DMPO). 10% of
An aqueous solution (20 μl) was added, followed by 1 mM hydrogen peroxide (H ₂
O ₂ ) was added and stirred. Radical addition of hydroxyl radicals generated from the addition of H ₂ O ₂ after 75 seconds (· OH) and (DMPO-OH) was measured using an electron spin resonance apparatus (ESR). The hydroxyl radical scavenging activity was determined on the assumption that the hydroxyl radical scavenging activity of distilled water was 0%. Table 1 shows the results. In the table, valerian, gymnema and amacha are all known as natural antioxidants. Hydrangea (scientific name: Hydrangea serrat)
a) Comparative control as a plant.

[0012]

[Table 1] ヒ ド ロ キ シ Hydroxy radical scavenging activity───────── ──────────────────────── Control (water) 0% Valeriana (10mg / ml) 8% Gymnema (10mg / ml) 23% Amacha (10mg / ml) ml) 30% Sakinoshita (10mg / ml) 60% ─────────────────────────────────

(2) Superoxide scavenging activity test To 5 μl of the sample (aqueous solution) shown in Table 2, 50 μl of a coloring reagent and 50 μl of an enzyme solution were added, and the mixture was allowed to stand at 37 ° C. for 20 minutes. The absorbance at was measured. However, the coloring reagent is a mixture of 0.1 M phosphate buffer pH 8, 0.4 mM xanthine, and 0.24 mM nitro blue tetrazolium. The enzyme solution is 0.049 U / ml xanthine oxidase. The reaction stop solution is 69 mM sodium dodecyl sulfate. The superoxide erasing activity was determined by converting it to the erasing rate by standard SOD. Table 2 shows the results.

[0014]

[Table 2] ───────────────────────────────── Superoxide scavenging activity ───────── ──────────────────────── Control (water) 0 U / ml Valeriana (10 mg / ml) 0 U / ml Gymnema (10 mg / ml) 0 U / ml amateur (10mg / ml) 7U / ml saxinosita (10mg / ml) 22U / ml ──────────────────────────── ─────

[0015]

The present invention will be described in more detail with reference to the following examples, but the present invention is not limited to these examples.

Example 1 Emulsion Stearic acid 2.5% by weight Cetyl alcohol 1.5 Vaseline 5.0 Liquid paraffin 7.0 Turtle oil 3.0 Polyoxyethylene (10 mol) monooleate 2.0 Polyethylene glycol 1500 3.0 Triethanolamine 1.0 1,3-butylene glycol extract of Saxifraga 0.02 Fragrance Appropriate amount Preservatives Appropriate amount Purified water residue (Production method) Add polyoxyethylene glycol 1500 and triethanolamine to ion-exchanged water and heat. Dissolve and maintain at 70 ° C. (aqueous phase). The other components are mixed, heated and melted and kept at 70 ° C. (oil phase). Add oil phase to water phase, emulsify uniformly with homomixer, and after emulsification, stir well
C. to give an emulsion.

Example 2 Cream Beeswax 10.0% by weight Cetyl alcohol 5.0 Hydrogenated lanolin 5.0 Squalane 37.5 Mink oil 3.0 Glyceryl monostearate 2.0 Polyoxyethylene (20 mol) Sorbitan monolaurin Acid Ester 2.0 Propylene Glycol 5.0 Echinacea extract of Saxifraga 0.02 Perfume Appropriate Preservative Appropriate Purified Water Residue (Production method) Add propylene glycol to ion-exchanged water,
Heat and maintain at 70 ° C. (aqueous phase). The other components are mixed, heated and melted and kept at 70 ° C. (oil phase). The oil phase was added to the water phase to carry out preliminary emulsification, and after uniform emulsification with a homomixer, the mixture was cooled to 30 ° C. while stirring well to obtain a cream.

Example 3 Cream Cetostearyl alcohol 3.5% by weight Squalane 40.0 Beeswax 3.0 Reduced lanolin 5.0 Ethyl paraben 0.3 Polyoxyethylene (20 mol) Sorbitan monopalmitate 2.0 Stearic acid Monoglycoside 2.0 30% ethanol extract of Saxifraga 5.0 Perfume 0.03 1,3-butylene glycol 5.0 Glycerin 5.0 Sodium hyaluronate 0.05 Purified water Residue (Production method) Cetostearyl alcohol, squalane, Beeswax, reduced lanolin, ethylparaben, polyoxyethylene (20 mol) sorbitan monopalmitate, stearic acid monoglycoside, saxifrage extract and perfume were heated and dissolved, and the mixture kept at 75 ° C was heated to 75 ° C.
1,3-butylene glycol, glycerin heated to
Add to sodium hyaluronate and purified water with stirring. After homogenizing the mixture and making the emulsified particles smaller,
The mixture was rapidly cooled with stirring to obtain a cream.

Example 4 Latex Acetone Extract of Saxifraga 1.0 wt% 1.5 stearic acid 1.5 cetyl alcohol 0.5 beeswax 2.0 polyoxyethylene (10 mol) monooleate 1.0 stearic acid monoglycoside 1 0.0 Quinseed extract (5% aqueous solution) 20.0 Propylene glycol 5.0 Ethanol 3.0 Ethylparaben 0.3 Methylparaben 0.05 Perfume 0.05 Purified water Residue (Production method) Acetone extract of Saxifraga in ethanol, Add flavor and dissolve (alcohol solution). Propylene glycol is added to purified water, and the mixture is dissolved by heating and maintained at 70 ° C. (aqueous phase). Mix other ingredients except quince seed extract,
Heat and dissolve and maintain at 70 ° C (oil phase). The oil phase is added to the water phase, pre-emulsified, and homogenized uniformly with a homomixer. While stirring, the alcohol solution and the quince seed extract are added. Thereafter, the mixture was rapidly cooled to 30 ° C. with stirring to obtain an emulsion.

[0020]

As described above, according to the present invention,
A remarkable antioxidant effect was able to be exhibited as compared with the conventional antioxidant, and an extremely strong antioxidant effect was also shown as compared with the same safflower family. Further, if the antioxidant of the present invention is used, even a substance which has been considered difficult to compound due to poor oxidation stability can be stably compounded in the system, and can be used in a wide range of cosmetics and foods. Use is expected.

──────────────────────────────────────────────────続 き Continued on the front page (51) Int.Cl. ⁶ Identification code Agency reference number FI Technical display location A61K 35/78 ADN A61K 35/78 ADN

Claims (2)

[Claims] The present invention relates to a Saxifragaceae family (scientific name: Saxi
fraga stolonifera) An antioxidant comprising a plant extract as an active ingredient. 2. The compounding amount of the Saxifraga plant extract is 0.0
The antioxidant according to claim 1, wherein the amount is from 01 to 20.0% by weight.