JPH1090148A - Apparatus for automatically staining microscope sample - Google Patents

Abstract

PROBLEM TO BE SOLVED: To increase/decrease a count of rinsing containers and chemical solution containers in accordance with an executed staining method by making a nozzle freely attachable/detachable to any one of discharge ports by a cap nut and allowing the discharge port without the nozzle fitted to be freely shut by a plug. SOLUTION: When a different count of rinsing containers is required, the count can be optionally changed within a count of discharge ports 17 formed in a font face of a water feed manifold 15. That is, when the rinsing containers are to be set, base end parts of nozzles 12 are fitted by cap nuts 16 to the discharge ports 17 of the count required by the rinsing containers. The nozzles 12 are inserted to bottom parts of the rinsing containers through insertion holes. On the other hand, plugs 25 are fitted at connection parts 21 where the discharge ports 17 without the nozzles 12 are opened, thereby to shut the discharge ports 17. A chemical solution container can be set at a part without the nozzles 12 in front of the water feed manifold 15.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION The apparatus for automatically staining microscope specimens according to the present invention is used in hospital clinics and various laboratories to obtain tissue fragments or cells (in the present specification, these are collectively used as samples). The present invention relates to an improvement in an automatic staining apparatus for a microscope specimen used for performing a staining process in order to observe a microscope.

[0002]

2. Description of the Related Art In hospitals and clinics, it is widely practiced to observe a sample excised from an affected part with a microscope to diagnose a disease. In order to facilitate diagnosis of such diseases by microscopic observation, the sample attached to the slide glass is stained, and various automatic staining devices that automatically perform this staining work are provided. Have been.

FIG. 8 shows an example of such an automatic dyeing apparatus described in Japanese Utility Model Laid-Open No. 5-66543. On the upper surface of the substrate provided in the upper part of the cabinet 1 in the horizontal direction, a large number of liquid storage containers 2 and 2 each having an upper opening and storing various chemicals or water for washing necessary for the dyeing treatment.
Are placed in an aligned state. Above the liquid storage containers 2 and 2, a movable beam 3 that can move horizontally in the horizontal direction (a direction in FIG. 8) of the substrate and a movable along the movable beam 3 to move the substrate forward and backward. There is provided a three-dimensional driving device including a movable column 4 that can move horizontally in the direction (the direction b in FIG. 8) and a hanging arm 5 that can move up and down along the movable column 4. A dyeing basket (omitted in FIG. 8) capable of accommodating a plurality of slide glasses to which a sample to be a microscope specimen is attached can be detachably attached to the suspension arm 5. Then, by lowering the hanging arm 5 in a state where the dyeing basket is hung on the hanging arm 5, the plurality of slide glasses stored in the dyeing basket are placed in the respective liquid storage containers 2, 2. It can be immersed in the stored chemical solution or cleaning water.

When a sample is stained by using the automatic staining apparatus as described above, first, a staining basket containing one or a plurality of slide glasses to which a sample to be stained is attached is attached to the suspension arm 5. Combine. As is well known, each slide glass is oriented vertically in a state of being stored in the dyeing basket. After the dyeing basket has been engaged with the suspension arm 5, the three-dimensional drive device is then driven. Then, the moving beam 3 and the moving column 4 and the suspension arm 5 constituting the three-dimensional driving device move based on a signal from a controller (not shown), and move the dyeing basket in a predetermined order for a predetermined time. Liquid storage containers 2, 2
Immerse in the chemical solution or washing water inside. The sample is stained by repeating such operations a predetermined number of times. In the example shown in FIG. 8, a chemical solution for replacement is stored in the cabinet 1 in order to automatically replace a frequently used chemical solution among the chemical solutions stored in the liquid storage containers 2 and 2. The structure which accommodated the tanks 6 and 6 shown in FIG. However, such tanks 6, 6 are not always necessary. In the case of such an automatic staining apparatus for microscope specimens that does not require the cabinet 1 for housing the tanks 6, 6, the following Japanese Patent Publication No.
As in the automatic staining apparatus for microscope specimens described in Japanese Patent Application Laid-Open No. 100522, the height can be configured to be set on a desk.

[0005] Furthermore, a multiple staining process, in which a plurality of stained baskets are processed in parallel using the above-described automatic staining apparatus for microscope specimens to stain more samples efficiently, is also disclosed in, for example, Japanese Patent Publication No. As described in Japanese Patent Publication No. 100522,
Conventionally known. That is, the dyeing basket containing the slide glass to which the sample is attached is immersed in the chemical solution for a predetermined time. During this immersion, the hanging arm 5 disengages from the dyeing basket, and another dyeing is performed. The other dyeing basket is put into a predetermined storage container 2 by engaging with the basket. Then, similarly, the engagement with the suspension arm 5 is released, and the other dyeing basket is immersed in the chemical solution for a predetermined time. These operations are performed in parallel on a plurality of dyeing baskets, and if there is a dyeing basket which has been immersed for a predetermined time, the hanging arm 5 engages with the dyeing basket, and is transferred to the next liquid storage container 2. Carry. By repeating such operations, a plurality of stained baskets can be processed in parallel, so that more samples can be stained in a shorter time than in the normal processing described above. Such multiple staining processing can be performed by storing the processing procedure in the controller in advance.

[0006]

In the case of the conventionally known automatic staining apparatus for microscope specimens constructed and operated as described above, a washing container for storing washing water among the storage containers 2 and 2 is used. The number of storage containers 2 and 2 used was fixed. That is, it is necessary to dispose a nozzle for ejecting the washing water at the bottom of the liquid storage containers 2 and 2 used as the washing container. In the case of the conventional structure, this nozzle is a fixed type.
For this reason, only the liquid storage containers 2 and 2 functioning as a washing container can be installed in the portion where the nozzle is provided, and the liquid storage containers 2 and 2 functioning as the washing container are installed in portions other than the portion where the nozzle is provided. could not.

As described above, since the number of the storage containers 2 and 2 used as the washing containers is fixed, even if the required number of washing containers changes due to a change in the dyeing method to be performed or the like.
I could not respond to this. That is, when the number of required washing containers changes due to a change in the dyeing method to be performed or the like, the number of the storage containers 2 and 2 used as the washing containers is changed, and the storage containers used as the chemical solution containers instead. 2,
It is preferable to change the number of 2 in order to carry out a smooth staining treatment by effectively using the liquid storage containers 2 and 2. However, in the case of the conventional structure, such a demand could not be met. The automatic staining apparatus for microscope specimens of the present invention has been invented in view of such circumstances.

[0008]

According to the present invention, there is provided an automatic microscope for staining a microscope specimen, which comprises a plurality of chemicals mounted on an upper surface of a substrate and storing therein a chemical for dyeing processing, similarly to the aforementioned conventional apparatus. A container, a washing container placed on the upper surface of the substrate and capable of storing therein washing water for washing therein, and a side wall of the washing container being penetrated in a watertight manner and inserted into the inner bottom of the washing container. A nozzle for jetting the washing water into the washing container, a water supply manifold connected to the base end of the nozzle, a dyeing basket capable of storing a plurality of slide glasses to which a sample to be a microscope specimen is attached, and And a driving device having a suspension arm that is capable of engaging and disengaging the dyeing basket in a vertical and horizontal direction. Then, the sample is immersed in the liquid in each of the storage containers by injecting the dyeing basket into the liquid storage container by the driving device at predetermined time intervals in a predetermined order, thereby dyeing the sample.

[0009] In particular, in the automatic microscope specimen staining apparatus of the present invention, a plurality of cylindrical discharge ports each having a male screw portion formed on the outer peripheral surface thereof are provided on the front surface of the water supply manifold. The washing containers are provided at the same pitch in the direction. The nozzle is detachably attached to any of the discharge ports with a cap nut, and the discharge port to which the nozzle is not attached is freely pluggable with a cap nut-shaped plug whose bottom is closed.

[0010]

In the automatic staining apparatus for microscope specimens of the present invention configured as described above, the number of washing containers can be arbitrarily changed within the range of the number of discharge ports provided on the front surface of the water supply manifold. it can. That is, when the washing container is provided, the base end portion of the nozzle is attached to the number of discharge ports that require the washing container with the cap nut. Then, this nozzle is inserted into the bottom of the washing container. On the other hand, a plug is attached to the discharge port to which the nozzle is not mounted, and the discharge port is closed.
Therefore, a chemical solution container can be installed in a portion where the nozzle is not mounted on the front side of the water supply manifold.

[0011]

1 to 7 show an embodiment of the present invention. The cabinet 7 is formed in a rectangular parallelepiped shape in which only the front surface is opened by covering the upper surface, the left and right side surfaces, and the rear surface (rear surface) of the frame with a panel and closing the lower surface with a lower plate 8. An upper plate 9 is fixed to an intermediate portion in the vertical direction of the cabinet 7 in parallel with the lower plate 8. On the upper surfaces of the lower plate 8 and the upper plate 9, a plurality of washing containers 10 and chemical solution containers 1 are respectively provided.
1 and 11 are placed.

On the inside of the lower part of the washing container 10,
A nozzle 12 for supplying cleaning water is inserted. For this purpose, an insertion hole 13 is provided in a portion of the wall surface of the washing container 10 which is aligned with the nozzle 12 as shown in FIG. 3, so that the nozzle 12 can be inserted into the inner bottom of the washing container 10. . When the washing container 10 is placed on the lower plate 8, the nozzle 12 is inserted into the lower inside of the washing container 10 through the insertion hole 13. The watertightness between the outer peripheral surface of the base end portion of the nozzle 12 and the inner peripheral edge of the insertion hole 13 in the state of being inserted in this manner is based on the elasticity of rubber, elastomer, or the like locked on the inner peripheral edge of the insertion hole 13. It is held by a seal ring 44 made of a material.

The nozzle 12 has a tubular shape with a closed top end and an open base end.
As shown in FIGS. 3 to 5, a large number of nozzle holes 14 are formed in the length direction of the nozzle 12. In a state where the dyeing basket 29 (see FIG. 2 described later) has entered the washing container 10, water is spouted from these nozzle holes 14, 14 toward the lower inner side surface of the washing container 10. A water flow is induced in the washing container 10. On the other hand, in each of the chemical solution containers 11, 11, a chemical solution necessary for performing a staining process on a sample attached to a slide glass housed in the staining basket 29 is stored.

In the case of the apparatus for automatically staining a microscope specimen according to the present invention, the nozzle 12 is disposed horizontally at the rear end of the upper surface of the lower plate 8, and is provided with a water supply manifold 15 which is connected to a water supply facility such as a water supply. On the front surface, a mechanism including a cap nut 16 and shown in detail in FIGS. That is, a plurality of (five in the illustrated example) connection portions 21 are provided on the front surface of the water supply manifold 15, and a discharge port is provided at the center of the distal end surfaces 22 of the connection portions 21, 21. 17 and 17 are opened. In addition, a water receiving port 28 for connecting a downstream end of a hose or the like leading to a water supply facility is provided on a lower surface of an intermediate portion of the water supply manifold 15. The base end of the nozzle 12 can be freely connected to each of the discharge ports 17. For this reason, the annular flange plate 1 is provided on the outer peripheral surface of the portion near the base end of the nozzle 12.
8 is externally fitted and fixed. Positioning pins 19 are planted on one surface of the flange plate 18 facing the discharge port 17, and positioning holes are provided at portions of the end surfaces 22, 22 where the positioning pins 19 can be aligned. 20, 20 are formed. These positioning pins 19 and positioning holes 20
The nozzles 12 engage with each other in a state where the base ends of the nozzles 12 are inserted into the discharge ports 17. And these positioning pins 19
The nozzle holes 14, 14 face sideways with the and the positioning holes 20 engaged with each other.

The base end of the nozzle 12 as described above is connected to the discharge port 1
7, the base end of the nozzle 12 is connected to the opening of the discharge port 17 with a seal plate 23 (FIG. 3) made of an elastic material externally fitted to the base end of the nozzle 12. Then, the positioning pin 19 and the positioning hole 20 are engaged.
Then, from the tip side of the nozzle 12, the nozzle 12
The cap nut 16 is externally fitted to the
It is screwed into a male screw part 24 formed on the outer peripheral surface of the connection part 21. As a result, the flange plate 18 and the seal plate 23 are
Nipped between the cap nut 16 and the tip end face 22,
The nozzle 12 can be connected to the discharge port 17 while maintaining watertightness. In addition, the water which squirted from the nozzle 12 into the washing container 10 and overflowed from the washing container 10 is
It flows out to the upper surface of the lower plate 8 and is discharged to a drainage facility through a drainage pipe (not shown).

On the other hand, the discharge ports 17, 17 to which the nozzle 12 is not mounted are closed by cap nut-shaped plugs 25, 25 whose bottoms are closed, and the chemical solution container is provided in front of the discharge ports 17, 17. 11 can be placed. That is, the cylindrical portions 26 forming the plugs 25, 25 are formed on the external thread portions 24, 24 on the outer peripheral surface of the connection portions 21, 21 in which the discharge ports 17, 17, on which the nozzle 12 is not mounted, are opened at the respective end surfaces. A female screw formed on the inner peripheral surface of the screw 26 is screwed together to close the discharge ports 17 and 17. Note that a seal plate is sandwiched between the inner surfaces of the closing plates 27, 27 constituting the plugs 25, 25 and the end surfaces of the connecting portions 21, 21 and closed by the plugs 25, 25. Discharge ports 17, 17
So that water does not leak from

As shown in FIG. 2, a dyeing basket 29 used in the present embodiment has a storage part 30 capable of storing a slide glass to which a sample to be dyed is adhered, and a base mounted on an upper part of the storage part 30. And a handle 31 whose end is pivotally supported. As is well known, the storage section 30 is capable of storing a plurality of slide glasses in a state where each slide glass stands in a vertical direction and a gap is interposed between adjacent slide glasses. The storage section 30 has a frame-like structure capable of supporting the slide glass, and is made to enter a liquid storage container storing liquid,
The stored slide glass can be immersed in this liquid.
Further, the handle portion 31 is provided between the swing arms 32, 32 pivotally supporting the base at the upper end of the outer surface of the storage portion 30, and extends between the swing arms 32, 32. Locking arm 33. Both ends of the locking arm 33 protrude from both ends of the storage section 30 in the length direction.
Locking projections 34 are formed on the lower surfaces of both ends of the locking arm 33, respectively.

When the slide glass is taken in and out of the storage section 30 of the dyeing basket 29 as described above, the swing arm 3
By swinging the bases 2 and 32 around their base ends, the locking arm 33 is retracted to the side from the upper end opening of the storage unit 30. On the other hand, when the slide glass on which the sample is stuck is stored in the storage section 30 and the sample is stained, as shown in FIG. Is located above the upper end opening.

On the other hand, a driving device 35 for moving the dyeing basket 29 in the cabinet 7 in the vertical and horizontal directions is provided in the cabinet 7. In the case of the present example, the driving device 35 is provided at the back end of the cabinet 7.
The cabinet 7 includes a movable column 37 that can move horizontally along a guide rail 36 disposed in the left-right direction of the cabinet 7, and a suspension arm 38 that can move up and down along the movable column 37. A mechanism for horizontally moving these moving columns 37 and the suspension arm 3
The mechanism for raising and lowering 8 can employ various well-known mechanisms. For example, a linear motor or an intermediate portion of a cable stretched between a drive pulley and a driven pulley is
7 or a cable drive mechanism coupled to the suspension arm 38 can be employed. In particular, the cable drive mechanism can be preferably used because the electric motor can be installed in a portion that is not exposed to the chemical vapor generated from the chemical liquid container 11. In any case, the driving device 35 moves the dyeing basket 29 by moving one or both of the moving pillar 37 and the suspension arm 38 for a predetermined time in a predetermined order for the lower plate 8 and the upper plate 8. The chemical solution containers 11 and 11 placed on the upper surface of the plate 9 and the washing container 10 are made to enter. Then, the sample adhered to the slide glass housed in the dyeing basket 29 is immersed in the chemical solution or the washing water stored in each of the containers 11, 10, and the sample is stained.

At the front and rear ends of the hanging arm 38, hooks 39, 39 are provided, which can lock both ends of the locking arm 33 of the dyeing basket 29, respectively. Such a hanging arm 38
When the dyeing basket 29 is lifted, the hooks 39 and 39 are moved to both ends of the locking arm 33 while the hanging arm 38 is moved to the lower side of the locking arm 33. The portion is made to enter a portion closer to the outside than the locking projections 34, 34. Next, the dyeing basket 29 can be lifted by raising the hanging arm 38. The operation for removing the dyeing basket 29 from the suspension arm 38 is performed in reverse.

As described above, the dyeing basket 29 is placed on the upper surface of the lower plate 8 so that the dye basket 29 enters the chemical solution containers 11, 11 and the washing container 10 placed on the upper surfaces of the lower plate 8 and the upper plate 9. A lower moving space 40 is provided between the upper end portions of the placed washing container 10 and chemical solution containers 11, 11 and the lower surface of the upper plate 9. Height H ₄₀ of the lower movement space 40, the Tsushitaude 38 hung above Senshokukago 29, this Senshokukago 29
Is large enough to move in the left-right direction without causing the lower end of the container to collide with the upper end of each of the containers 10 and 11.
Further, the chemical solution containers 11, 11 placed on the upper surface of the upper plate 9
An upper moving space 42 is provided between the cabinet 7 and the lower surface of the top plate 41 that partitions the upper surface of the cabinet 7. Height H ₄₂ of the upper moving space 42, the Tsushitaude 38 hung above Senshokukago 29, left and right without impinging a lower end portion of the Senshokukago 29 to the upper end of each container 10, 11 It is large enough to move in any direction. Further, a rectangular passage 43 is provided at the center of the upper plate 9 in the left-right direction, through which the hanging arm 38 hanging the dyeing basket 29 can pass along the dyeing basket 29 in the vertical direction. I have.

To stain a sample attached to a plurality of slide glasses stored in the staining basket 29 by using the microscope specimen automatic staining apparatus of the present invention configured as described above, first, use the slide glass. Is engaged with the suspension arm 38. This engagement work is performed, for example, in a state where the dyeing basket 29 is inserted into any of the washing containers 10.
This is performed by moving the hanging arm 38 above the dyeing basket 29. Next, the moving column 37 and the suspension arm 38 constituting the driving device are appropriately driven to drive the dyeing basket 29.
The drug solution container 11 for a predetermined time according to a predetermined order,
11 is immersed in the chemical solution or the washing water in the washing container 10.
The sample is stained by repeating such operations a predetermined number of times.

That is, the suspension arm 38 is attached to the lower moving space 4.
The dyeing basket 29 is placed on the upper surface of the lower plate 8 by washing or lifting the dyeing basket 29 by moving up or down above the predetermined washing container 10 or the chemical solution container 11 while moving in the left or right direction through the zero or upper moving space 42. It is immersed in the chemicals in the containers 11, 11 or the chemical containers 11, 11 placed on the upper surface of the upper plate 9. As the dyeing process performed in this manner proceeds,
From the chemical solution in the chemical solution container 11 placed on the upper surface of the upper plate 9 to the cleaning water in the washing container 10 or the chemical solution in the chemical solution container 11 placed on the upper surface of the lower plate 8 or the lower plate It is necessary to transfer from the washing water in the washing container 10 placed on the upper surface of the upper plate 8 or the chemical solution in the chemical solution container 11 to the chemical solution in the chemical solution container 11 placed on the upper surface of the upper plate 9 respectively. At this time, the hanging arm 38 keeps the dyeing basket 29 suspended,
It passes between the lower moving space 40 and the upper moving space 42 through the passage 43 provided at the center in the left-right direction of the upper plate 9.

When a plurality of dyeing baskets 29 are used to simultaneously proceed with the same or different kinds of dyeing methods, any of the dyeing baskets 29 is washed with any of the washing containers 10 or the chemical solution containers 1.
1 and the hanging arm 38 from the dyeing basket 29.
Is removed, and the other dyeing basket 29 is moved. On this occasion,
Prior to removing the hanging arm 38 from the dyeing basket 29, the hanging arm 38 is finely moved up and down, and the dyeing basket 29 is shaken in washing water or a chemical solution to form a slide glass housed in the dyeing basket 29. Allow the adhered sample to mix with the wash water or chemical.

By changing the staining method performed by the automatic staining apparatus for microscope specimens configured and operating as described above, or by changing the number of staining methods performed simultaneously.
When the number of required washing containers 10 changes, the discharge ports 17, 17 provided on the front of the water supply manifold 15 are provided.
The number of the washing containers 10 can be changed arbitrarily within the range of the number (the number is within five in the illustrated example, but the number can be increased). That is, when the washing container 10 is provided, as described above, the base end of the nozzle 12 is attached to the required number of discharge ports 17 of the washing container 10 by the cap nut 16. Then, the nozzle 12 is inserted into the bottom of the washing container 10 through the insertion hole 13. On the other hand, plugs 25, 25 are attached to the connection portions 21, 21 where the discharge ports 17 to which the nozzles 12 are not mounted are opened, and the discharge ports 17 are closed. Therefore, the chemical solution containers 11 and 11 can be installed in a portion where the nozzle 12 is not mounted on the front side of the water supply manifold 15.

In the illustrated example, the upper plate 9 is fixed to a frame constituting the cabinet 7, and the lower moving space 4 is fixed.
0 and the height dimensions H ₄₀ and H of both the upper moving space 42.
_42, the Tsushitaude 38 hung Senshokukago 29 and the lower end of the Senshokukago 29 as the size of only movable in the horizontal direction without colliding to the upper end of each container 10, 11 . On the other hand, the upper plate 9 can be supported on the frame so as to be able to move up and down in a horizontal state. Then, the upper plate 9 is lowered in a state where the suspension arm 38, on which the dyeing basket 29 is suspended, is moved above the upper plate 9 through the passage 43, and the height dimension of the upper moving space 42 is secured (this state). Therefore, the height of the lower moving space 40 can be reduced.), The height of the automatic staining device for microscope specimens can be reduced.

Further, the liquid storage containers such as the water washing container 10 and the chemical liquid container 11 can be arranged in three or more upper and lower stages. That is, at least one intermediate plate (not shown) having a passage 43 similar to that of the upper plate 9 is provided between the upper plate 9 and the lower plate 8,
A plurality of liquid medicine containers 11 are also placed on the upper surface of the intermediate plate.
In this case, the lower moving space 40 is provided between the upper ends of the washing container 10 and the chemical liquid containers 11, 11 placed on the upper surface of the lower plate 8 and the lower surface of the intermediate plate. Further, the upper end of the chemical solution container 11 placed on the upper surface of the intermediate plate and the upper plate 9
Of the hanging arm 3 on which the dyeing basket 29 is suspended
8 is provided with an intermediate moving space that can move in the left-right direction.

Also, with the above structure, the intermediate plate is supported on the frame so as to be able to move up and down in a horizontal state, and the hanging arm 38 on which the dyeing basket 29 is suspended moves above the intermediate plate through the passage 43. If the intermediate plate is lowered to secure the height of the intermediate moving space, the height of the automatic staining device for microscope specimens can be reduced. Furthermore, if the upper plate 9 can be moved up and down with such a structure, the height of the automatic staining device for microscope specimens can be further reduced.

In any of the above structures, the upper plate 9
Alternatively, the passage 43 provided in the intermediate plate may be formed at the end of the upper plate 9 and the intermediate plate, not at the center in the left-right direction. In particular, if the passage 43 is provided above the washing container 10 placed on the upper surface of the lower plate 8, when the hanging arm 38 suspending the dyeing basket 29 moves vertically through the passage 43, Even if the chemical solution attached to the dyeing basket 29 and the slide glass housed in the dyeing basket 29 drips, the dripped chemical solution only enters the lower washing container 10 but enters the other chemical solution containers 11. Without deteriorating other chemicals. Since the washing water in the washing container 10 is constantly replaced by the ejection of the new washing water from the nozzle 12, dripping of a small amount of the chemical solution hardly causes a problem. Note that an empty container may be provided below the passage 43 to receive the dripped chemical.

A plurality of sets of moving columns 37 and hanging arms 38 are provided in the automatic dyeing apparatus.
8 may be moved in the right half and the left half including the center in the left-right direction of each moving space. With this configuration, simultaneous processing of a plurality of dyed baskets can be performed smoothly without difficulty.

[0031]

The automatic staining apparatus for microscope specimens of the present invention
Although it is configured and operates as described above, the number of washing containers and chemical containers can be appropriately increased or decreased according to the dyeing method to be performed, so that a smooth dyeing process can be performed.

[Brief description of the drawings]

FIG. 1 is a schematic perspective view of an entire automatic staining apparatus, showing an example of an embodiment of the present invention.

FIG. 2 is a schematic perspective view of the dyeing basket.

FIG. 3 is an enlarged sectional view taken on line AA of FIG. 1;

FIG. 4 is an exploded perspective view of a portion where a nozzle or a plug is mounted on a water supply manifold.

FIG. 5 is a partially cut side view showing a nozzle.

FIG. 6 is a partial front view of a water supply manifold.

FIG. 7 is a sectional view taken along line BB of FIG. 6;

FIG. 8 is a perspective view showing an example of a conventional device.

[Explanation of symbols]

 DESCRIPTION OF SYMBOLS 1 Cabinet 2 Liquid storage container 3 Moving beam 4 Moving column 5 Hanging arm 6 Tank 7 Cabinet 8 Lower plate 9 Upper plate 10 Rinsing container 11 Chemical liquid container 12 Nozzle 13 Insertion hole 14 Nozzle hole 15 Water supply manifold 16 Bag nut 17 Discharge port 18 Flange plate 19 Positioning pin 20 Positioning hole 21 Connection part 22 Tip surface 23 Seal plate 24 Male screw part 25 Plug 26 Cylindrical part 27 Closing plate part 28 Water receiving port 29 Stain basket 30 Storage part 31 Handle part 32 Swing arm part 33 Locking arm Portion 34 Locking projection 35 Drive device 36 Guide rail 37 Moving column 38 Hanging arm 39 Hook 40 Lower moving space 41 Top plate 42 Upper moving space 43 Passage 44 Seal ring

Claims (1)

[Claims] 1. A plurality of chemical solution containers mounted on an upper surface of a substrate and storing therein a chemical solution for dyeing processing, and a cleaning water mounted on the upper surface of the substrate and capable of storing cleaning water therein for cleaning. A washing container, and a nozzle for ejecting washing water into the washing container by inserting one side wall of the washing container in a watertight manner and inserting the washing water into the washing container, and a base end portion of the nozzle. A water supply manifold, a dyeing basket capable of storing a plurality of slide glasses to which a sample to be a microscope specimen is attached, and a driving device having a hanging arm capable of freely disengaging and disengaging the dyeing basket in the vertical and horizontal directions. A microscope for immersing the sample in the liquid in each of the storage containers by moving the dyeing basket into the liquid storage container for a predetermined time in a predetermined order by the driving device, and dyeing the sample. In automatic staining equipment for specimens On the front surface of the water supply manifold, a plurality of cylindrical discharge ports each having an external thread formed on the outer peripheral surface thereof are provided at an equal pitch with the flush containers over the direction in which the flush containers are arranged. The nozzle can be attached to or detached from any of the outlets with a cap nut, and the outlet without a nozzle can be closed with a cap-shaped plug with a closed bottom. Dyeing equipment.