JPH111409A - Antimicrobial agent and method for producing the same - Google Patents

Abstract

(57) [Summary] [PROBLEMS] To provide an antibacterial agent which can contribute to the effective use of plum trees and is excellent in safety. SOLUTION: The pruned branch of a pruned plum tree is processed into flakes and steamed at 130 ° C. using steam. afterwards,
The extract is immersed in pure water and cooled, and the extract of the peduncle is extracted into pure water. Then, the elongate branches after the extraction are removed by filtration with a filter, and the filtrate is recovered as an extract.
This extract has excellent antibacterial properties and has no safety problems, and can be used as an antibacterial agent. This extract is also characterized by having a favorable aroma of plum. And since this antibacterial agent is water-soluble, it can be used for food preservatives, water-based paints, etc. In addition, it can be used for cleaning agents for removing slime from kitchens, antibacterial members, etc. ,
Its applicability is wide.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

The present invention relates to an antibacterial agent and a method for producing the same, and more particularly, to an antibacterial agent containing an extract from a branch of a plum tree or the like and a method for producing the same.

[0002]

2. Description of the Related Art Conventionally, a large number of plum trees have been cultivated to obtain edible plums. In the cultivation of plum trees, prunus shoots are pruned every fall, which is a huge amount of thousands to tens of thousands of tons per year. At present, the pruned pruned branches are not effectively used and are incinerated as waste. Most of the seeds and husks after collecting the plum extract and plum meat are also discarded. This is not desirable from the viewpoint of effective use of resources and environmental conservation. The problem of the effective use of the plum tree is not limited to the above-mentioned buds and seed shells, but should be solved, for example, in the trunk, branches, leaves, roots, etc. of the plum tree.

On the other hand, in recent years, with the improvement of living environment and changes in the concept of personal cleanliness, various antibacterial agents and products provided with antibacterial properties have been developed. In addition, it is necessary to sufficiently consider the safety of the human body for these antibacterial agents and antibacterial products.

[0004]

SUMMARY OF THE INVENTION Accordingly, an object of the present invention is to provide an antibacterial agent which can contribute to the effective use of a ume tree and is excellent in safety and a method for producing the same.

[0005]

In order to achieve the above object, the antibacterial agent of the present invention comprises a plum tree trunk, a plum tree branch, a plum tree leaf, a plum tree root, a plum meat, a plum seed shell and a plum kernel. The extract from at least one selected from the group consisting of:

That is, the present inventor has conducted a series of studies on the effective use of ume trees as a main subject, and as a result, has found that an extract of water, etc., of an elongate branch of a ume tree has an antibacterial effect.
The present invention has been reached. The present inventor has conducted similar studies on other trees such as citrus trees, but extracts of ume tree elongate branches and the like have extremely high antibacterial activity compared to other tree extracts. It can be put to practical use. In addition, the extract of the plum tree has no problem in safety to the human body, and since it has a scent of plum, the antibacterial agent of the present invention using the same is excellent in safety and has a preferable scent. . Further, the antibacterial agent of the present invention also has the property of being easily dissolved in water, and therefore has a wide range of uses, and can be used as, for example, a food preservative or added to a water-based paint, as described later.

[0007] The antibacterial agent of the present invention may be composed of only the extract (100%). In the present invention, “antibacterial” includes both “bacteriostatic” and “sterilization”.

In the present invention, the extract preferably contains catechin. Catechin is a substance having an antibacterial action. In the extract according to the present invention, the substance having an antibacterial effect is not limited to the catechin. In the present invention, the form of the antibacterial agent is preferably a powder or liquid.

Next, there are the following four production methods for producing the antibacterial agent of the present invention.

[0010] First, the first production method of the present invention comprises at least one selected from the group consisting of a plum tree trunk, a plum tree branch, a plum tree leaf, a plum tree root, a plum meat, a plum seed shell and a plum kernel. Is flaked, steamed with heated steam, then immersed in water and cooled, and the water is recovered as an extract.

[0011] The second production method of the present invention is characterized in that at least one selected from the group consisting of a plum tree trunk, a plum tree branch, a plum tree leaf, a plum tree root, a plum meat, a plum seed shell and a plum kernel. Is flaked, boiled with water and cooled, and the cooled water is recovered as an extract.

[0012] The third production method of the present invention is characterized in that at least one selected from the group consisting of a plum tree trunk, a plum tree branch, a plum tree leaf, a plum tree root, a plum meat, a plum seed shell and a plum kernel. Is flaked, immersed in an organic solvent, and the immersed organic solvent is recovered as an extract.

[0013] Further, a fourth production method of the present invention is characterized in that at least one selected from the group consisting of a plum tree trunk, a plum tree branch, a plum tree leaf, a plum tree root, a plum meat, a plum seed shell and a plum kernel. In a flake form, which is subjected to dry distillation, and the distillate is recovered as an extract. The carbonization includes carbonization. That is, the carbonization of wood or the like is performed by blocking air and heating. In this carbonization, the distillate is recovered as an extract.

Next, the antibacterial member of the present invention contains the antibacterial agent of the present invention. Further, it is preferable that the member is at least one member of a group consisting of fiber, cloth, board, paper, resin, and wall.

Next, the cleaning agent of the present invention contains the antibacterial agent of the present invention. This is because the antibacterial agent of the present invention has an action of removing slime generated in toilets, kitchens, bathrooms, and the like in addition to the antibacterial action.

Next, the antibacterial paint of the present invention contains the above antibacterial agent of the present invention. Since the extract according to the present invention has high solubility in water, for example, the antibacterial property can be imparted by blending the antibacterial agent of the present invention into an aqueous paint.

Next, the food preservative of the present invention contains the antibacterial agent of the present invention. As described above, the antibacterial agent of the present invention contains an extract such as a prunus branch of a plum tree,
Because there is no problem in safety for the human body, it can be added to food.

[0018]

BEST MODE FOR CARRYING OUT THE INVENTION The antibacterial agent of the present invention can be produced, for example, by the first production method as follows.

First, the pruned branches of a plum tree are processed into flakes. The size of the flakes is usually the size of sawdust. The stamens used are pruned. Processing into a flake shape can be performed using, for example, a wood cutter.

Next, the flakes are steamed with heated steam, and the temperature is usually about 100 to 200 ° C, preferably about 130 ° C. The heating time is usually 1 minute to 3 hours.

After the heating, it is immersed in water and cooled. During this cooling, antibacterial components and the like are extracted from the elongate branches and the like. The cooling temperature is usually about 0-30 ° C, preferably 0-20 ° C. The water used for cooling is not particularly limited, but is preferably water that can be used for beverages such as tap water, and particularly preferably pure water such as distilled water.

Then, the flakes are filtered by a filter or the like, and the filtrate is recovered as an extract. This extract may be used as it is as an antibacterial agent, or other components may be added to make it an antibacterial agent. The concentration of the active ingredient in the extract is usually 0.01 to 99.99% by weight, preferably 1 to 30% by weight, as a dry weight ratio. When the concentration of the active ingredient in the extract is lower than the predetermined concentration, the extract may be concentrated by boiling down, and when it exceeds the predetermined concentration, the extract may be diluted with pure water or the like.

The extract may be used as it is as a liquid antibacterial agent, or may be processed into a powder by spray drying, freeze drying, or the like. For this processing, a commonly used spray dryer or freeze dryer may be used.

Next, an example of the production of an antibacterial agent according to the second production method will be described below.

First, ume tree elongate branches are flaked by the above-mentioned method. Then, it is boiled with hot water.
The condition of the boiling is usually from 80 to 100 ° C for 1 minute to 3 hours, preferably from 95 to 100 ° C for 30 minutes to 1 hour. The hot water used for boiling is particularly preferably pure water, as described above. At the time of boiling, antibacterial components and the like are extracted from the stamens and the like. Then, after boiling, cool. Examples of the cooling method include, in addition to natural cooling, a method in which the outer wall of a pot used for boiling is brought into contact with cold water. If a boiling pot with a jacket for temperature control is used for boiling, the boiling and cooling steps can be performed smoothly. Then, the extracted branch is separated by a filter or the like, and the extract is collected. The subsequent steps, etc.
This is the same as the first manufacturing method described above.

Next, a production example of an antibacterial agent according to the third production method will be described below.

First, ume tree elongate branches are flaked by the above-mentioned method. Then, this is immersed in an organic solvent. The type of the organic solvent is not particularly limited, and includes, for example, methanol, ethanol, hexane, acetone, ethyl acetate, glycerin, propylene glycol, etc. Among them, ethanol is preferred from the viewpoint of safety and availability. The organic solvent used for the extraction is not limited to one type, and may be used in combination of two or more types, or may be used by mixing with water. For example, in the case of a mixture of alcohol and water, the alcohol concentration is usually 20 to 99.
It is 95% by weight, preferably 70 to 99.95% by weight. The immersion time is usually 1 minute to 3 hours.
The immersion temperature is not particularly limited, and may be, for example, room temperature. During this immersion, antibacterial components and the like are extracted from the elongate branches and the like. Then, the extracted branch is separated by a filter or the like, and the extract is collected. The subsequent steps and the like are the same as in the above-described first manufacturing method.

Next, a production example of an antibacterial agent according to the fourth production method will be described below.

First, ume tree elongate branches are flaked by the above-mentioned method. Then, this is subjected to heat treatment (dry distillation) while shutting off the air. The heating temperature at this time is usually 90
To 300 ° C., and the heating time is usually 1 to 3 hours. For this dry distillation, for example, a retort pot, a dry distillation pot, or the like can be used. The liquid distilled by the dry distillation is separated from the above-mentioned twigs and the like as an extract and collected. The extract obtained by carbonization has a high concentration of the active ingredient.
The subsequent steps and the like are the same as in the above-described first manufacturing method.

Next, the use of this antibacterial agent will be described.

First, the liquid antibacterial agent can be used, for example, as a spray-type antibacterial agent. The spray type antibacterial agent, for example, by spraying on the wall of a house, furniture, daily necessities, meat, fish field, foods such as vegetables, etc., can sterilize these things, and can also impart antibacterial properties, Further, a preferable scent of plum can be imparted. In addition, it can be added as a food preservative at the time of producing food, and in this case, in addition to the preservative action, a favorable aroma of plum can be imparted. In addition, an antibacterial water-based paint can be obtained by adding it to the water-based paint. This antibacterial water-based paint,
There is no danger of decay even when stored for a long period of time. In addition, antibacterial properties and plum aroma can be imparted to those coated with this paint.

Next, the powdered antibacterial agent of the present invention can be used as a cleaning agent for removing slime generated in water places such as kitchens, bathrooms and toilets. If this cleaning agent is used, slime can be removed and bacteria can be removed, and the kitchen and the like become sanitary. A surfactant, a cleaning aid, and the like may be added to this cleaning agent, as in a general cleaning agent. Further, this powdered antibacterial agent can be used when producing an antibacterial member. For example, when manufacturing antibacterial synthetic fibers,
What is necessary is just to add the said powdery antimicrobial agent to the raw material resin.

[0033]

EXAMPLE Next, an example will be described.

(Example 1) A pruning tree of a plum tree was cut using a wood cutter to obtain flakes of a growing tree. And
The flakes were steamed with steam at 130 ° C. for 3 hours, and then immersed in cold water (pure water) at about 20 ° C. for cooling. Then, the flakes were removed using a filter, and the filtrate was collected as an extract.

The thus obtained extract was subjected to an antibacterial test by the following colony counting method. The test results are also shown below.

(Test strain) E. coli: Escherichia coli (E. coli) IF
O 3301 Staphylococcus aureus: Staphylococcus aureus (S. aureus) IFO 12732 MRSA: Staphylococcus aureus (S. au)
reus) IID 1677 Pseudomonas aeruginosa: Pseudomonas aeruginosa (P. aeru
ginosa) IFO 13275

(Medium for measuring the number of bacteria) SCDLP agar medium (manufactured by Nippon Pharmaceutical Co., Ltd.)

(Preparation of Bacterial Solution) (1) Escherichia coli, Staphylococcus aureus and MRSA in a normal agar slant medium (manufactured by Eiken Chemical Co., Ltd.) at 35 ° C.
The cells of the test strain cultured for 6 to 20 hours are sterilized by 1/10
The suspension was suspended in a 0-concentration ordinary broth and prepared so that the number of bacteria per ml was about 10 ⁶ . (2) Pseudomonas aeruginosa on a normal agar slant medium (manufactured by Eiken Chemical Co., Ltd.) at 35 ° C.
The cells of the test strain cultured for 6 to 20 hours are sterilized by 1/10
The suspension was suspended in a normal broth having a concentration of 00 and prepared so that the number of bacteria per ml was about 10 ⁶ .

(Test operation) The above extract was applied to the surface of a plastic plate (length 50 mm x width 50 mm) and dried.
This was used as a sample. The sample was inoculated with 0.5 ml of a bacterial solution, and adhered to a polyethylene film, and then stored at 35 ° C. for 6 hours, and the surviving bacteria were washed out with 10 ml of the SCDLP medium. The number of bacteria per 1 ml of the wash solution was measured by an agar plate culture method (35 ° C., 2 days) using a medium for measuring the number of bacteria, and converted into the number of viable bacteria per sample. As a control, a petri dish was inoculated with the same amount of bacteria as that inoculated to the sample, and the same operation as described above was performed.
The number of bacteria at the start of storage was based on a control. Also,
These operations were performed twice for each sample or control.

(Escherichia coli) Number of control 1 colonies: 5.3 × 10 ⁵ immediately after storage, 6.2 × 10 ⁴ after 6 hours storage 7.1 × 10 ⁵ immediately after storage, 7.0 × 10 ⁴ samples after 6 hours storage Number of 1 colony (after 6 hours of storage): 9.0 × 10 ³ 6.7 × 10 ⁴ Sample Number of 2 colonies (after 6 hours of storage): <10 <10

(Staphylococcus aureus) Number of colonies per control: 5.4 × 10 ⁵ immediately after storage, 1.4 × 10 ⁵ after 6 hours storage 5.3 × 10 ⁵ immediately after storage, 3.1 × 10 after 6 hours storage ⁵ samples 1 colony count (after 6 hours of storage): 1.1 × 10 ⁵ 9.0 × 10 ⁴ samples 2 colonies count (after 6 hours of storage): 5.6 × 10 ⁴ 4.5 × 10 ⁴

(MRSA) Control 1 colony count: 3.3 × 10 ⁵ immediately after storage, 1.2 × 10 ⁴ after 6 hours storage 5.7 × 10 ⁵ immediately after storage, 4.7 × 10 ⁴ samples after 6 hours storage Number of 1 colony (after 6 hours of storage): 1.2 × 10 ⁵ 9.6 × 10 ⁴ Sample 2 Number of colonies (after 6 hours of storage): 8.9 × 10 ³ 8.8 × 10 ³

(Pseudomonas aeruginosa) Control 1 colony number: 3.2 × 10 ⁵ immediately after storage, 1.2 × 10 ⁴ after 6 hours storage 3.1 × 10 ⁵ immediately after storage, 7.6 × 10 after 6 hours storage Number of ⁵ samples per colony (after 6 hours of storage): 1.2 × 10 ⁵ 1.5 × 10 ⁵ Number of samples 2 colonies (after 6 hours of storage): 3.4 × 10 ³ 9.7 × 10 ²

From the above results, it can be seen that the extract of the elongate branch of the plum tree has an excellent antibacterial activity.

[0045]

As described above, the antibacterial agent of the present invention comprises at least one selected from the group consisting of a plum tree trunk, a plum tree branch, a plum tree leaf, a plum tree root, a plum meat, a plum seed shell and a plum tree. Contains extracts from one. This extract has an excellent antibacterial action and has no safety problems. Further, the antibacterial agent of the present invention has a preferable aroma of plum. Therefore, use of the antibacterial agent of the present invention can contribute to making daily life hygienic and comfortable. Further, since the antibacterial agent of the present invention is water-soluble, it can be applied to food preservatives, antibacterial waterborne paints, and the like. Further, the antibacterial agent of the present invention can be used to impart antibacterial properties to members such as cloths and plates. Further, the antibacterial agent of the present invention uses one that has been discarded in the cultivation of plum trees, such as a stamen tree. Therefore, implementation of the present invention can contribute to effective use of resources and environmental conservation.

──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. ⁶ Identification code FI C09D 7/12 C09D 7/12 Z C11D 3/382 C11D 3/382 3/48 3/48

Claims (14)

[Claims] 1. An antibacterial agent comprising an extract from at least one selected from the group consisting of a plum tree trunk, a plum tree branch, a plum tree leaf, a plum tree root, a plum meat, a plum seed shell and a plum kernel. 2. The antibacterial agent according to claim 1, wherein the extract contains catechin. 3. The antibacterial agent is in a powder form.
Or the antibacterial agent according to 2. 4. The antibacterial agent according to claim 1, wherein the antibacterial agent is in a liquid form. 5. A flake form of at least one selected from the group consisting of a plum tree trunk, a plum tree branch, a plum tree leaf, a plum tree root, a plum meat, a plum seed shell and a plum tree, A method for producing an antibacterial agent in which steam is then immersed in water and cooled, and the water is recovered as an extract. 6. A flake of at least one selected from the group consisting of a plum tree trunk, a plum tree branch, a plum tree leaf, a plum tree root, a plum meat, a plum seed shell and a plum tree, and boiled with water. And then cooling and recovering the cooled water as an extract. 7. A flake form of at least one selected from the group consisting of a plum tree trunk, a plum tree branch, a plum tree leaf, a plum tree root, a plum meat, a plum seed shell and a plum tree, and converting it into an organic solvent. A method for producing an antibacterial agent that is immersed and recovers the immersed organic solvent as an extract. 8. A flake of at least one selected from the group consisting of a plum tree trunk, a plum tree branch, a plum tree leaf, a plum tree root, a plum meat, a plum seed shell and a plum tree, and carbonized,
A method for producing an antibacterial agent that collects the distillate as an extract. 9. An antibacterial member containing the antibacterial agent according to any one of claims 1 to 4. 10. The member is at least one member of the group consisting of fiber, cloth, board, paper, resin, and wall.
The antibacterial member according to the above. A cleaning agent containing the antibacterial agent according to any one of claims 1 to 4. 12. The cleaning agent according to claim 11, which has at least one use for kitchens, toilets and bathrooms. 13. A food preservative containing the antibacterial agent according to any one of claims 1 to 4. 14. An antibacterial paint containing the antibacterial agent according to claim 1. Description: