JPH1180202A - Fucoidan of different acetic acid content from natural and cultured okinawa mozuku and its manufacture - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a fucoidan of different acetic acid content from natural and cultured Okinawa mozuku as a base material and its manufacture. SOLUTION: A fucoidan substituting a lot of acetic acid can be manufactured by extracting the fucoidan with water or an acid from Okinawa mozuku (natural and cultured). But, it was found that if the Okinawa mozuku is treated with an alkali, a fucoidan contg. a little acetic acid group is obtd. (L-fucose: D-xylose: D-glucuronic acid: acetic acid: sulfuric acid = 3.5-5.0: 0.1-0.3: 0.5-1.5: 0.0-1.5: 0.4-1.5). This fucoidan has the same physical and chemical properties with the one extracted with water or an acid and can be applicable in an extensive field.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION The present invention relates to the supply of low cholesterol, anti-gastric ulcer, anti-AIDS virus, antibacterial agent, moisturizer, thickener, and L-fucose from natural and cultured Okinawa mozuku. The present invention relates to the simple production of fucoidan with different degrees of substitution of acetate groups, which can be used in pharmaceuticals, functional foods, cosmetics, biotechnology and other industries.

[0002]

BACKGROUND OF THE INVENTION A patent for the production and production of acetylfucoidan from cultured Okinawa mozuku was filed (Japanese Patent Application No. 8-359852; Japanese Patent Application No. 9-285948). However, it was found that the acetate group to be substituted by fucoidan was easily dissociated by an alkali. Therefore, in the present invention, the state of the acetate group was examined under various alkali conditions in addition to water and acid.

[0003]

The applicant has revealed that large quantities of fucoidan are present in Okinawa mozuku (approximately 2.0
%; Per wet algal body). The northern limit of growth of Okinawa mozuku is Amami Oshima in Kagoshima Prefecture and the southern limit is Ishigaki Island in Okinawa Prefecture. Therefore, this algae is a promising biological resource in Okinawa Prefecture. Earlier, fucoidan was produced from Okinawa mozuku and its chemical composition was examined. As a result, it was invented that fucoidan was largely substituted by an acetate group. However, since this acetate group was found to be easily dissociated under alkaline conditions, the purpose of the present invention was to clarify the relationship between the degree of substitution of the acetate group and the alkali concentration.

[0004]

The inventors of the present invention have focused on the above-mentioned problems and prepared an oak mozuku whose cell wall was destroyed using a mixer or a homogenizer into an alkali (0.01-0.50 mol) solution to extract fucoidan. After neutralization with an acid (room temperature, 2 hours), fucoidan was produced by spray drying, alcohol, or lyophilization. Table 1 shows 0.0
The chemical analysis values of fucoidan when treated with 12 mol of sodium hydroxide are shown. The yield was calculated for the wet alga body. Total sugar and uronic acid contents were determined by phenol sulfate and carbazole sulfate methods. Furthermore, the sulfuric acid content was determined by the barium precipitation method. L-fucose and D-xylose were determined by liquid chromatography. The spectrum of ¹ HNMR (500 MHz: manufactured by JEOL) of fucoidan treated under these conditions is shown in FIG. From the area ratio of the signal of L-fucose and the methyl proton signal of acetic acid, the composition ratio of both is about 9.
0: 1.0. The constituent ratio of L-fucose and acetic acid of fucoidan extracted with water or acid is 5.0: 1.
Since the ratio was 0 or 4.8: 1.0, the amount of acetic acid was reduced to about 1/2 under the above alkali conditions. When fucoidan was extracted from Okinawa mozuku at different alkali concentrations (0.01-0.50 mol), the content of acetate groups fluctuated significantly. That is, when Okinawa mozuku was treated with a highly alkaline solution, acetate groups were significantly reduced (for example,
0.2 mol sodium hydroxide, room temperature, stirring for 2 hours, 0%). Fig. 1 ¹ H-NMR spectrum of fucoidan extracted from Okinawa mozuku with weak alkali

[0005] Table 2 shows the calculated molecular ratios of fucoidan obtained by treatment with various concentrations of alkaline solutions in addition to fucoidan extracted with water.

As described above, when the natural and cultured Okinawa mosques were extracted with water or acid, and also with various concentrations of alkaline solutions, the amount of acetate groups fluctuated significantly. However, these fucoidans only vary significantly in the amount of acetic acid, do not change their properties as fucoidans, and can be supplied in large quantities to many fields (medicine, food, cosmetics, and other industries).

──────────────────────────────────────────────────の Continued on the front page (51) Int.Cl. ⁶ Identification code FI A61K 31/725 ADN A61K 31/725 ADN ADZ ADZ 35/80 ADU 35/80 ADUZ

Claims (2)

[Claims] The present invention relates to natural and cultured Okinawan oats (Clad).
osiphonokamuranus TOKIDA)
From the alga bodies and spores (discoids) of L. fucose, D-xylose, D-glucuronic acid, acetic acid, and sulfuric acid, having a molecular composition ratio of 3.5 to 5.0:
0.1-0.3: 0.5-1.5: 0.0: 1.5:
Fucoidan consisting of 0.5-1.5. 2. After aquaculture or natural Okinawa mozuku is treated with a mixer or homogenizer, water, acid or alkali is added to stir and extract fucoidan. In the latter case, after neutralization, diatomaceous earth filtration or continuous centrifugal separator is used. A method for producing fucoidan having a different degree of substitution of acetic acid groups using a spray dryer, alcohol or freeze dryer.