JPS5526870A - Method of measuring activity of gamma-glutamyl transpeptidase - Google Patents
Method of measuring activity of gamma-glutamyl transpeptidaseInfo
- Publication number
- JPS5526870A JPS5526870A JP10015678A JP10015678A JPS5526870A JP S5526870 A JPS5526870 A JP S5526870A JP 10015678 A JP10015678 A JP 10015678A JP 10015678 A JP10015678 A JP 10015678A JP S5526870 A JPS5526870 A JP S5526870A
- Authority
- JP
- Japan
- Prior art keywords
- glutamyl transpeptidase
- gtp
- gamma
- formula
- lower alkyl
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 101710107035 Gamma-glutamyltranspeptidase Proteins 0.000 title abstract 2
- 101710173228 Glutathione hydrolase proenzyme Proteins 0.000 title abstract 2
- 230000000694 effects Effects 0.000 title abstract 2
- 102000006640 gamma-Glutamyltransferase Human genes 0.000 title abstract 2
- 101000856500 Bacillus subtilis subsp. natto Glutathione hydrolase proenzyme Proteins 0.000 abstract 2
- 125000000217 alkyl group Chemical group 0.000 abstract 2
- 125000001140 1,4-phenylene group Chemical group [H]C1=C([H])C([*:2])=C([H])C([H])=C1[*:1] 0.000 abstract 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 abstract 1
- 238000004040 coloring Methods 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 230000007613 environmental effect Effects 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
- 125000000472 sulfonyl group Chemical group *S(*)(=O)=O 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
PURPOSE: A specific substance is used to measure the activity of γ-GTP in living samples accurately and rapidly without particular consideration on environmental safety, thus permitting the high sensibility of color development and instantaneous coloring reaction.
CONSTITUTION: A γ-glutamyl-p-aminoanilide derivative of formula I (R1, R2 are lower alkyl; R3 is H, lower alkyl, carboxyl, sulfonyl group) is used as the substrate to form the p-phenylene derivative of formula II by the action of γ- glutamyl transpeptidase (γ-GTP) in liveing samples. The product is oxidized or oxidatively polymerized in the coexistence of a coupler to form colored compounds, which are colorimetrically determined.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10015678A JPS5526870A (en) | 1978-08-17 | 1978-08-17 | Method of measuring activity of gamma-glutamyl transpeptidase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP10015678A JPS5526870A (en) | 1978-08-17 | 1978-08-17 | Method of measuring activity of gamma-glutamyl transpeptidase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5526870A true JPS5526870A (en) | 1980-02-26 |
Family
ID=14266450
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP10015678A Pending JPS5526870A (en) | 1978-08-17 | 1978-08-17 | Method of measuring activity of gamma-glutamyl transpeptidase |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5526870A (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS56158745A (en) * | 1980-05-13 | 1981-12-07 | Dai Ichi Pure Chem Co Ltd | L-gamma-glutamyl-3-carboxy-4-aminoanilide derivative and its preparation |
| JPS56164796A (en) * | 1980-05-20 | 1981-12-17 | Dai Ichi Pure Chem Co Ltd | Measurement of activity of gamma-glutamyltranspeptidase |
| JPS592764A (en) * | 1982-06-26 | 1984-01-09 | 中松 義郎 | Energy transmitting golf club |
| JPS59130199A (en) * | 1982-09-17 | 1984-07-26 | ベ−リンガ−・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング | Method and reagent for measuring -glutanul transferase |
| JPS59501535A (en) * | 1982-08-30 | 1984-08-30 | ベツクマン・インストルメンツ・インコ−ポレ−テツド | A method for determining the activity of γ-glutamyl transferase and a kit containing a novel substrate solution for the method |
| JPS59177462U (en) * | 1983-05-16 | 1984-11-27 | 勝俣 信久 | Golf club |
| JPS61136767U (en) * | 1985-02-12 | 1986-08-25 |
-
1978
- 1978-08-17 JP JP10015678A patent/JPS5526870A/en active Pending
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS56158745A (en) * | 1980-05-13 | 1981-12-07 | Dai Ichi Pure Chem Co Ltd | L-gamma-glutamyl-3-carboxy-4-aminoanilide derivative and its preparation |
| JPS56164796A (en) * | 1980-05-20 | 1981-12-17 | Dai Ichi Pure Chem Co Ltd | Measurement of activity of gamma-glutamyltranspeptidase |
| JPS592764A (en) * | 1982-06-26 | 1984-01-09 | 中松 義郎 | Energy transmitting golf club |
| JPS59501535A (en) * | 1982-08-30 | 1984-08-30 | ベツクマン・インストルメンツ・インコ−ポレ−テツド | A method for determining the activity of γ-glutamyl transferase and a kit containing a novel substrate solution for the method |
| JPS59130199A (en) * | 1982-09-17 | 1984-07-26 | ベ−リンガ−・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング | Method and reagent for measuring -glutanul transferase |
| JPS59177462U (en) * | 1983-05-16 | 1984-11-27 | 勝俣 信久 | Golf club |
| JPS61136767U (en) * | 1985-02-12 | 1986-08-25 |
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