JPS57166999A - Determination of cholinesterase activity - Google Patents

Determination of cholinesterase activity

Info

Publication number
JPS57166999A
JPS57166999A JP5170781A JP5170781A JPS57166999A JP S57166999 A JPS57166999 A JP S57166999A JP 5170781 A JP5170781 A JP 5170781A JP 5170781 A JP5170781 A JP 5170781A JP S57166999 A JPS57166999 A JP S57166999A
Authority
JP
Japan
Prior art keywords
nadph
hydroxybenzoylcholine
hydroxybenzoic acid
cholinesterase
activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP5170781A
Other languages
Japanese (ja)
Other versions
JPS603838B2 (en
Inventor
Yoshihiro Ashihara
Yasushi Kasahara
Masami Sugiyama
Takahiro Harada
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujirebio Inc
Original Assignee
Fujirebio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Fujirebio Inc filed Critical Fujirebio Inc
Priority to JP5170781A priority Critical patent/JPS603838B2/en
Priority to EP82300965A priority patent/EP0060059B1/en
Priority to DE8282300965T priority patent/DE3265517D1/en
Priority to ES510091A priority patent/ES510091A0/en
Publication of JPS57166999A publication Critical patent/JPS57166999A/en
Publication of JPS603838B2 publication Critical patent/JPS603838B2/en
Expired legal-status Critical Current

Links

Landscapes

  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PURPOSE: To determine the activity of the titled substance, by adding a solution containing hydroxybenzoylcholine, m-hydroxybenzoic acid hydroxylase and NADPN to the specimen, and determining the light absorbance of the consumed NADPH by colorimetry.
CONSTITUTION: The hydroxybenzoylcholine of formula is used as the synthetic substrate, and a solution containing hydroxybenzoylcholine, m-hydroxybenzoic acid hydroxylase and NADPH is added to a liquid containing cholinesterase. The m-hydroxybenzoic acid produced from the hydroxybenzoylcholine by the action of the cholinesterase is converted to dihydroxybenzoic acid by the action of the m-hydroxybenzoic acid hydroxylase in the presence of NADPH. The amount of the consumed NADPH is determined as the decrease of the light absorbance of NADPH by colorimetry, and the cholinesterase activity is calculated from the value.
COPYRIGHT: (C)1982,JPO&Japio
JP5170781A 1981-03-04 1981-04-08 Method for measuring cholinesterase activity Expired JPS603838B2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP5170781A JPS603838B2 (en) 1981-04-08 1981-04-08 Method for measuring cholinesterase activity
EP82300965A EP0060059B1 (en) 1981-03-04 1982-02-25 Method for determining the activity of cholinesterase and diagnostic solution for use therein
DE8282300965T DE3265517D1 (en) 1981-03-04 1982-02-25 Method for determining the activity of cholinesterase and diagnostic solution for use therein
ES510091A ES510091A0 (en) 1981-03-04 1982-03-03 "A METHOD TO DETERMINE CHOLINESTERASE ACTIVITY".

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP5170781A JPS603838B2 (en) 1981-04-08 1981-04-08 Method for measuring cholinesterase activity

Publications (2)

Publication Number Publication Date
JPS57166999A true JPS57166999A (en) 1982-10-14
JPS603838B2 JPS603838B2 (en) 1985-01-30

Family

ID=12894362

Family Applications (1)

Application Number Title Priority Date Filing Date
JP5170781A Expired JPS603838B2 (en) 1981-03-04 1981-04-08 Method for measuring cholinesterase activity

Country Status (1)

Country Link
JP (1) JPS603838B2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60238000A (en) * 1984-05-10 1985-11-26 Nitto Boseki Co Ltd Novel method of cholinesterase activity measurement

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS60238000A (en) * 1984-05-10 1985-11-26 Nitto Boseki Co Ltd Novel method of cholinesterase activity measurement

Also Published As

Publication number Publication date
JPS603838B2 (en) 1985-01-30

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