JPS5554894A - Novel formaldehyde-dehydrogenase and its preparation - Google Patents
Novel formaldehyde-dehydrogenase and its preparationInfo
- Publication number
- JPS5554894A JPS5554894A JP12656878A JP12656878A JPS5554894A JP S5554894 A JPS5554894 A JP S5554894A JP 12656878 A JP12656878 A JP 12656878A JP 12656878 A JP12656878 A JP 12656878A JP S5554894 A JPS5554894 A JP S5554894A
- Authority
- JP
- Japan
- Prior art keywords
- formaldehyde
- enzyme
- dehydrogenase
- preparation
- pseudomonas
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 102100039702 Alcohol dehydrogenase class-3 Human genes 0.000 title 1
- 108010051015 glutathione-independent formaldehyde dehydrogenase Proteins 0.000 title 1
- 102000004190 Enzymes Human genes 0.000 abstract 3
- 108090000790 Enzymes Proteins 0.000 abstract 3
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 abstract 3
- BAVYZALUXZFZLV-UHFFFAOYSA-N Methylamine Chemical compound NC BAVYZALUXZFZLV-UHFFFAOYSA-N 0.000 abstract 2
- 241000589516 Pseudomonas Species 0.000 abstract 2
- DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 abstract 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 abstract 2
- FSYKKLYZXJSNPZ-UHFFFAOYSA-N sarcosine Chemical compound C[NH2+]CC([O-])=O FSYKKLYZXJSNPZ-UHFFFAOYSA-N 0.000 abstract 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 abstract 1
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 abstract 1
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 abstract 1
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 abstract 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 abstract 1
- 108010077895 Sarcosine Proteins 0.000 abstract 1
- LEHOTFFKMJEONL-UHFFFAOYSA-N Uric Acid Chemical compound N1C(=O)NC(=O)C2=C1NC(=O)N2 LEHOTFFKMJEONL-UHFFFAOYSA-N 0.000 abstract 1
- TVWHNULVHGKJHS-UHFFFAOYSA-N Uric acid Natural products N1C(=O)NC(=O)C2NC(=O)NC21 TVWHNULVHGKJHS-UHFFFAOYSA-N 0.000 abstract 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 abstract 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 abstract 1
- 235000011130 ammonium sulphate Nutrition 0.000 abstract 1
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 229960003237 betaine Drugs 0.000 abstract 1
- 239000008280 blood Substances 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 abstract 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 1
- 229940109239 creatinine Drugs 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 235000019253 formic acid Nutrition 0.000 abstract 1
- 238000004255 ion exchange chromatography Methods 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 230000002503 metabolic effect Effects 0.000 abstract 1
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 229950006238 nadide Drugs 0.000 abstract 1
- 230000003647 oxidation Effects 0.000 abstract 1
- 238000007254 oxidation reaction Methods 0.000 abstract 1
- 239000002243 precursor Substances 0.000 abstract 1
- 238000005185 salting out Methods 0.000 abstract 1
- 229940043230 sarcosine Drugs 0.000 abstract 1
- 229940116269 uric acid Drugs 0.000 abstract 1
Landscapes
- Enzymes And Modification Thereof (AREA)
Abstract
NEW MATERIAL:The novel title enzyme produced by the culturing of bacterial belonging to Pseudomonas genus, and capable of irreversibly producing formic acid by the oxidation of formaldehyde through nicotinamide-adenine dinucletide. Molecular weight, 15,000±1,000; isoelectric point, 5.2±0.1; optimum pH, 7W8.
USE: Reagent for the determination of uric acid in blood.
PROCESS: Bacteria belonging to Pseudomonas genus and capable of producing the above enzyme, e.g. Pseudomonas aeruginosa IFO3445, are inoculated in a medium containing 0.05W1wt% of metabolic precursors such as sarcosine, betaine, methylamine, creatinine, formaldehyde, etc., and cultured at 15W40°C and pH6W8 for 1W2 days. The title enzyme is extracted from the medium and purified by salting- out with ammonium sulfate, ion exchange chromatography, etc.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12656878A JPS5554894A (en) | 1978-10-13 | 1978-10-13 | Novel formaldehyde-dehydrogenase and its preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP12656878A JPS5554894A (en) | 1978-10-13 | 1978-10-13 | Novel formaldehyde-dehydrogenase and its preparation |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP6740579A Division JPS5552944A (en) | 1979-05-30 | 1979-05-30 | Novel quantitizing method of formaldehyde with formaldehyde dehydrogenase |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5554894A true JPS5554894A (en) | 1980-04-22 |
| JPS6155946B2 JPS6155946B2 (en) | 1986-11-29 |
Family
ID=14938376
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP12656878A Granted JPS5554894A (en) | 1978-10-13 | 1978-10-13 | Novel formaldehyde-dehydrogenase and its preparation |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5554894A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60186297A (en) * | 1984-03-06 | 1985-09-21 | Oriental Yeast Co Ltd | Removal of nad+ activation inhibitor |
-
1978
- 1978-10-13 JP JP12656878A patent/JPS5554894A/en active Granted
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60186297A (en) * | 1984-03-06 | 1985-09-21 | Oriental Yeast Co Ltd | Removal of nad+ activation inhibitor |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS6155946B2 (en) | 1986-11-29 |
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