JPS5559A - Preparation of immobilized bio-active substance - Google Patents
Preparation of immobilized bio-active substanceInfo
- Publication number
- JPS5559A JPS5559A JP7177078A JP7177078A JPS5559A JP S5559 A JPS5559 A JP S5559A JP 7177078 A JP7177078 A JP 7177078A JP 7177078 A JP7177078 A JP 7177078A JP S5559 A JPS5559 A JP S5559A
- Authority
- JP
- Japan
- Prior art keywords
- collagen
- carrier
- bioactive substance
- group
- spacer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000000975 bioactive effect Effects 0.000 title abstract 5
- 239000013543 active substance Substances 0.000 title 1
- 102000008186 Collagen Human genes 0.000 abstract 7
- 108010035532 Collagen Proteins 0.000 abstract 7
- 229920001436 collagen Polymers 0.000 abstract 7
- 239000000126 substance Substances 0.000 abstract 4
- 125000006850 spacer group Chemical group 0.000 abstract 3
- 125000000524 functional group Chemical group 0.000 abstract 2
- 125000005647 linker group Chemical group 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- IVRMZWNICZWHMI-UHFFFAOYSA-N azide group Chemical group [N-]=[N+]=[N-] IVRMZWNICZWHMI-UHFFFAOYSA-N 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 238000005886 esterification reaction Methods 0.000 abstract 1
- 239000000835 fiber Substances 0.000 abstract 1
- 150000004820 halides Chemical class 0.000 abstract 1
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Peptides Or Proteins (AREA)
Abstract
PURPOSE: To prepare an immobilized bioactive substance having the specific biological characteristics of collagen and the activity of the bioactive substance, by bonding collagen carrier with the bioactive substance through a compound (spacer) having a proper length.
CONSTITUTION: Bonding groups (e.g. azide group) are introduced into a coolagen carrier such as a molded soluble collagen, insolubilized collagen fiber, etc. e.g. by reacting with a cyan halide. The collagen carrier is pref. subjected to a proper pretreatment, e.g. methyl-esterification, to activate the functional groups of the carrier. A spacer having a functional group such as NH2 group is introduced to the collagen carrier having the bonding group, and the bioactive substance (e.g. enzyme, antibody) is chemically bonded and immobilized to the collagen carrier through the spacer.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7177078A JPS5559A (en) | 1978-06-14 | 1978-06-14 | Preparation of immobilized bio-active substance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP7177078A JPS5559A (en) | 1978-06-14 | 1978-06-14 | Preparation of immobilized bio-active substance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPS5559A true JPS5559A (en) | 1980-01-05 |
Family
ID=13470112
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP7177078A Pending JPS5559A (en) | 1978-06-14 | 1978-06-14 | Preparation of immobilized bio-active substance |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS5559A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58192829A (en) * | 1982-05-07 | 1983-11-10 | Seikagaku Kogyo Co Ltd | Immobilized physiologically active substance and its preparation |
| JPS61207340A (en) * | 1985-03-13 | 1986-09-13 | Koken:Kk | Treatment of collagen substrate |
| JPS63304000A (en) * | 1987-06-05 | 1988-12-12 | Ube Ind Ltd | Immobilization method for biological substances |
| JPH0222300A (en) * | 1987-02-02 | 1990-01-25 | Swiss Serum & Vaccine Inst Bern | Immunogen conjugate, its production and generation of immune |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS52139781A (en) * | 1976-05-15 | 1977-11-21 | Nippi Inc | Production of fixed biologically active substance |
| JPS5341489A (en) * | 1976-08-12 | 1978-04-14 | Boehringer Sohn Ingelheim | Production of enzyme preparation |
-
1978
- 1978-06-14 JP JP7177078A patent/JPS5559A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS52139781A (en) * | 1976-05-15 | 1977-11-21 | Nippi Inc | Production of fixed biologically active substance |
| JPS5341489A (en) * | 1976-08-12 | 1978-04-14 | Boehringer Sohn Ingelheim | Production of enzyme preparation |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS58192829A (en) * | 1982-05-07 | 1983-11-10 | Seikagaku Kogyo Co Ltd | Immobilized physiologically active substance and its preparation |
| JPS61207340A (en) * | 1985-03-13 | 1986-09-13 | Koken:Kk | Treatment of collagen substrate |
| JPH0222300A (en) * | 1987-02-02 | 1990-01-25 | Swiss Serum & Vaccine Inst Bern | Immunogen conjugate, its production and generation of immune |
| JPS63304000A (en) * | 1987-06-05 | 1988-12-12 | Ube Ind Ltd | Immobilization method for biological substances |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JPS54157816A (en) | Fixing of bioactive substance to solid surface | |
| FR2452485B1 (en) | NOVEL 4-AMINO-3-QUINOLEINECARBOXYLIC ACIDS AND ESTERS, ESPECIALLY USEFUL AS ANTISECRETORY AND ANTI-ULCER AGENTS | |
| DE3789839D1 (en) | Production of enzymes with changed activity. | |
| JPS57130922A (en) | Preparation of anti-human protein antibody | |
| FR2534272B1 (en) | PROCESS FOR PRODUCING AN IMMOBILIZED ENZYME PREPARATION USING A CROSSLINKING AGENT, PREPARATION OF IMMOBILIZED ENZYME THUS OBTAINED, AND USE THEREOF | |
| ES453362A1 (en) | Carrier-bound biologically active substances and process for their manufacture | |
| IT7927440A0 (en) | IMMOBILIZED ENZYME, PROCEDURES FOR THE PREPARATION OF SAID ENZYME AS WELL AS PROCEDURE FOR CARRYING OUT AN ENZYME REACTION. | |
| JPS5559A (en) | Preparation of immobilized bio-active substance | |
| AU7912887A (en) | Monoclonal antibodies to fibrin | |
| AU525518B2 (en) | Immobilized glucose isomerase | |
| JPS5598912A (en) | Glucose-isomerizing fiber and its production | |
| AU7649187A (en) | Preparation of monoclonal antibodies | |
| EP0203095A4 (en) | Process for the preparation of polyamide supports capable of reacting with antibodies and enzymes and the supports thus produced. | |
| DE3869465D1 (en) | PRODUCTION OF 4-PENTENOATE BY ISOMERISATION. | |
| Watabe et al. | Studies on enzymatic hydrolysis of aziridines. I. The conversion of 2 beta, 3beta-imino-5 alpha-cholestane into 2 beta-amino-3-alpha-hydroxy-5 alpha-cholestane by liver microsomes | |
| JPS6420087A (en) | Immobilization of enzyme or bacterium cell using chitosan-aldehyde gel as carrier | |
| JPS5257390A (en) | Immobilized enzyme and method of preparing the same | |
| JPS53124682A (en) | Preparation of complex of enzyme and bioactive substance and its use | |
| JPS5785320A (en) | Immobilized immuno-active substance and its preparation | |
| JPS5437883A (en) | Preparation of immobilized enzyme | |
| JPS5257392A (en) | Immobilized enzyme using fibroin as carrier and method of preparing th e same | |
| FI903504A0 (en) | FOERFARANDE FOER BESTAEMNING AV ANTIGENER OCH / ELLER ANTIKROPPAR I MAENNISKANS KROPPSVAETSKOR SAMT PROVSET FOER GENOMFOERANDE AV FOERFARANDET. | |
| JPS6430650A (en) | Carrier for immobilizing biological active substance | |
| JPS5294488A (en) | Preparing of nucleic acid-enzyme complex | |
| JPS5795904A (en) | Cockroach attractant |