JPS6420087A - Immobilization of enzyme or bacterium cell using chitosan-aldehyde gel as carrier - Google Patents
Immobilization of enzyme or bacterium cell using chitosan-aldehyde gel as carrierInfo
- Publication number
- JPS6420087A JPS6420087A JP17574787A JP17574787A JPS6420087A JP S6420087 A JPS6420087 A JP S6420087A JP 17574787 A JP17574787 A JP 17574787A JP 17574787 A JP17574787 A JP 17574787A JP S6420087 A JPS6420087 A JP S6420087A
- Authority
- JP
- Japan
- Prior art keywords
- chitosan
- enzyme
- aldehyde
- bacterium cell
- gel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000894006 Bacteria Species 0.000 title abstract 6
- 102000004190 Enzymes Human genes 0.000 title abstract 6
- 108090000790 Enzymes Proteins 0.000 title abstract 6
- 229920001661 Chitosan Polymers 0.000 abstract 3
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 abstract 2
- 150000001299 aldehydes Chemical class 0.000 abstract 2
- 239000011347 resin Substances 0.000 abstract 2
- 229920005989 resin Polymers 0.000 abstract 2
- 239000002262 Schiff base Substances 0.000 abstract 1
- 150000004753 Schiff bases Chemical class 0.000 abstract 1
- 230000004931 aggregating effect Effects 0.000 abstract 1
- 125000003277 amino group Chemical group 0.000 abstract 1
- 238000004132 cross linking Methods 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Landscapes
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
PURPOSE:To immobilize an enzyme or a bacterium cell in high productivity, by adding chitosan-aldehyde gel to the enzyme or bacterium cell, then crosslinking the gel with glutaraldehyde making into a resin in a gelatinous state and aggregating. CONSTITUTION:1pt.wt. chitosan is reacted with 1-50pts.wt. of an aldehyde to form Schiff base from amino group of part of chitosan and the aldehyde. Consequently, chitosan-aldehyde gel having a structure shown by the formula [R is H or -(CH2)nCH3; n is 0-2] and a water content corresponding to 10-1,000 times the empty weight of chitosan is produced. Then the chitosan- aldehyde gel is added to an enzyme or bacterium cell, the enzyme or bacterium cell is taken in the surface or the interior of the chitosan-aldehyde gel, which is crosslinked with glutaraldehyde, made into a resin in a gelatinous state and aggregated to immobilize the enzyme or bacterium cell.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17574787A JPS6420087A (en) | 1987-07-13 | 1987-07-13 | Immobilization of enzyme or bacterium cell using chitosan-aldehyde gel as carrier |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17574787A JPS6420087A (en) | 1987-07-13 | 1987-07-13 | Immobilization of enzyme or bacterium cell using chitosan-aldehyde gel as carrier |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS6420087A true JPS6420087A (en) | 1989-01-24 |
| JPH0327198B2 JPH0327198B2 (en) | 1991-04-15 |
Family
ID=16001545
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP17574787A Granted JPS6420087A (en) | 1987-07-13 | 1987-07-13 | Immobilization of enzyme or bacterium cell using chitosan-aldehyde gel as carrier |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS6420087A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5489401A (en) * | 1991-11-20 | 1996-02-06 | Ramot University Authority For Applied Research & Industrial Development Ltd. | Method for entrapment of active materials in chitosan |
| WO2007045139A1 (en) * | 2005-10-19 | 2007-04-26 | Institute Of Oceanology Chinese Academy Of Sciences | Agricultural bactericides and the use thereof |
| CN120959329A (en) * | 2025-09-05 | 2025-11-18 | 贝嘉美(天津)生物技术研发股份有限公司 | A compound feed additive and its preparation method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5745555A (en) * | 1980-09-01 | 1982-03-15 | Hitachi Metals Ltd | Electric charge type magnetic toner |
| JPS5849234A (en) * | 1981-09-18 | 1983-03-23 | Bridgestone Corp | Molder for tire |
| JPS5849234U (en) * | 1981-09-30 | 1983-04-02 | 株式会社日立製作所 | Multi-slit spectrophotometer |
| JPS59213390A (en) * | 1983-05-19 | 1984-12-03 | Asahi Denka Kogyo Kk | Immobilized enzyme and its preparation |
-
1987
- 1987-07-13 JP JP17574787A patent/JPS6420087A/en active Granted
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5745555A (en) * | 1980-09-01 | 1982-03-15 | Hitachi Metals Ltd | Electric charge type magnetic toner |
| JPS5849234A (en) * | 1981-09-18 | 1983-03-23 | Bridgestone Corp | Molder for tire |
| JPS5849234U (en) * | 1981-09-30 | 1983-04-02 | 株式会社日立製作所 | Multi-slit spectrophotometer |
| JPS59213390A (en) * | 1983-05-19 | 1984-12-03 | Asahi Denka Kogyo Kk | Immobilized enzyme and its preparation |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5489401A (en) * | 1991-11-20 | 1996-02-06 | Ramot University Authority For Applied Research & Industrial Development Ltd. | Method for entrapment of active materials in chitosan |
| WO2007045139A1 (en) * | 2005-10-19 | 2007-04-26 | Institute Of Oceanology Chinese Academy Of Sciences | Agricultural bactericides and the use thereof |
| CN1312991C (en) * | 2005-10-19 | 2007-05-02 | 中国科学院海洋研究所 | Agricultural bactericidal agent |
| CN120959329A (en) * | 2025-09-05 | 2025-11-18 | 贝嘉美(天津)生物技术研发股份有限公司 | A compound feed additive and its preparation method |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0327198B2 (en) | 1991-04-15 |
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