JPS5564800A - Method and reagent for determination of acyl coenzyme a - Google Patents
Method and reagent for determination of acyl coenzyme aInfo
- Publication number
- JPS5564800A JPS5564800A JP13701178A JP13701178A JPS5564800A JP S5564800 A JPS5564800 A JP S5564800A JP 13701178 A JP13701178 A JP 13701178A JP 13701178 A JP13701178 A JP 13701178A JP S5564800 A JPS5564800 A JP S5564800A
- Authority
- JP
- Japan
- Prior art keywords
- acyl coenzyme
- reagent
- oxidase
- measurement
- coenzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- -1 acyl coenzyme a Chemical compound 0.000 title abstract 4
- 239000003153 chemical reaction reagent Substances 0.000 title abstract 4
- 229940093530 coenzyme a Drugs 0.000 title abstract 4
- 238000000034 method Methods 0.000 title abstract 2
- RGJOEKWQDUBAIZ-UHFFFAOYSA-N coenzime A Natural products OC1C(OP(O)(O)=O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-UHFFFAOYSA-N 0.000 abstract 3
- 239000005516 coenzyme A Substances 0.000 abstract 3
- KDTSHFARGAKYJN-UHFFFAOYSA-N dephosphocoenzyme A Natural products OC1C(O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 KDTSHFARGAKYJN-UHFFFAOYSA-N 0.000 abstract 3
- 102000004539 Acyl-CoA Oxidase Human genes 0.000 abstract 2
- 108020001558 Acyl-CoA oxidase Proteins 0.000 abstract 2
- 239000007788 liquid Substances 0.000 abstract 2
- 238000005259 measurement Methods 0.000 abstract 2
- 102000016938 Catalase Human genes 0.000 abstract 1
- 108010053835 Catalase Proteins 0.000 abstract 1
- 102000003992 Peroxidases Human genes 0.000 abstract 1
- JBFYJLIMSJROLD-UHFFFAOYSA-N [4,4-bis(dimethylamino)cyclohexa-1,5-dien-1-yl]-phenylmethanol Chemical compound C1=CC(N(C)C)(N(C)C)CC=C1C(O)C1=CC=CC=C1 JBFYJLIMSJROLD-UHFFFAOYSA-N 0.000 abstract 1
- 239000008280 blood Substances 0.000 abstract 1
- 210000004369 blood Anatomy 0.000 abstract 1
- 238000011088 calibration curve Methods 0.000 abstract 1
- 235000021588 free fatty acids Nutrition 0.000 abstract 1
- PGLTVOMIXTUURA-UHFFFAOYSA-N iodoacetamide Chemical compound NC(=O)CI PGLTVOMIXTUURA-UHFFFAOYSA-N 0.000 abstract 1
- 125000005439 maleimidyl group Chemical class C1(C=CC(N1*)=O)=O 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 230000003287 optical effect Effects 0.000 abstract 1
- 230000001590 oxidative effect Effects 0.000 abstract 1
- 108040007629 peroxidase activity proteins Proteins 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
PURPOSE: To determine acyl coenzyme A useful for the clinical measurement of free fatty acids in blood, easily, accurately and rapidly, by measuring the H2O2 produced by the oxidative activity of acyl coenzyme A-oxidase.
CONSTITUTION: A liquid specimen containing acyl coenzyme A is adjusted to pH 7.0W8.5, and added with an acyl coenzyme A-oxidase, an SH reagent, a peroxidase or catalase, and a color developing reagent, separately or at a time. The mixture is heated at 37°C for 5W60min in an open atmosphere, and the optical density is measured using a light corresponding to the color of the liquid, e.g. a light having a wave length of 400W600nm. The amount of the acyl coenzyme A is determined from the calibration curve prepared by the process similar to the above. The addition of the SH reagent (a maleimide derivative, iodoacetamide, 4,4-bisdimethylamino-benzhydrol, etc.) improves the sensitivity of the measurement.
COPYRIGHT: (C)1980,JPO&Japio
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13701178A JPS5564800A (en) | 1978-11-06 | 1978-11-06 | Method and reagent for determination of acyl coenzyme a |
| DE19792944498 DE2944498C2 (en) | 1978-11-06 | 1979-11-03 | Method for the quantitative determination of acyl-coenzyme A and reagent therefor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP13701178A JPS5564800A (en) | 1978-11-06 | 1978-11-06 | Method and reagent for determination of acyl coenzyme a |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP4346381A Division JPS578797A (en) | 1981-03-24 | 1981-03-24 | Method and reagent for quantitative determination of free fatty acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS5564800A true JPS5564800A (en) | 1980-05-15 |
| JPS5729998B2 JPS5729998B2 (en) | 1982-06-25 |
Family
ID=15188720
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP13701178A Granted JPS5564800A (en) | 1978-11-06 | 1978-11-06 | Method and reagent for determination of acyl coenzyme a |
Country Status (2)
| Country | Link |
|---|---|
| JP (1) | JPS5564800A (en) |
| DE (1) | DE2944498C2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5743699A (en) * | 1980-08-25 | 1982-03-11 | Yatoron:Kk | Measuring method of free fatty acid |
| US6686172B2 (en) | 2000-06-30 | 2004-02-03 | Azwell, Inc. | Method of measuring total homocysteine |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5685300A (en) * | 1979-12-12 | 1981-07-11 | Amano Pharmaceut Co Ltd | Determination of free fatty acid |
| JPS56158097A (en) * | 1980-05-06 | 1981-12-05 | Toyobo Co Ltd | Quantitative determination of free fatty acid |
| JPS60217900A (en) * | 1984-04-13 | 1985-10-31 | Kyowa Medetsukusu Kk | Method for determination of mercapto group-containing compound |
| JP4257730B2 (en) * | 2003-08-25 | 2009-04-22 | キッコーマン株式会社 | Acyl CoA oxidase, its gene, recombinant DNA, and method for producing acyl CoA oxidase |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5217085A (en) * | 1975-07-30 | 1977-02-08 | Ono Pharmaceut Co Ltd | Method of quantitative determination of free fatty acids in serum usin g fatty acid activating enzymes |
-
1978
- 1978-11-06 JP JP13701178A patent/JPS5564800A/en active Granted
-
1979
- 1979-11-03 DE DE19792944498 patent/DE2944498C2/en not_active Expired
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5743699A (en) * | 1980-08-25 | 1982-03-11 | Yatoron:Kk | Measuring method of free fatty acid |
| US6686172B2 (en) | 2000-06-30 | 2004-02-03 | Azwell, Inc. | Method of measuring total homocysteine |
Also Published As
| Publication number | Publication date |
|---|---|
| JPS5729998B2 (en) | 1982-06-25 |
| DE2944498C2 (en) | 1985-08-29 |
| DE2944498A1 (en) | 1980-05-14 |
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